Increased flexibility, pain reduction and unaltered levels of IL-10 and CD11b + lymphocytes in patients with systemic lupus erythematosus were associated with kinesiotherapy.

The effect of physical activity on the immune system is still poorly understood in cases of systemic lupus erythematosus (SLE). Therefore, our aim was to investigate differences in the serum levels of cytokines (IL-2, IL-5, IL-6, IL-8, IL-10 and TNF-α) and the numbers of CD11b + and CXCR2 + neutrophils and lymphocytes in women with SLE undergoing drug treatment, without ( n = 9) or with ( n = 5) 4 months of kinesiotherapy. Parameters related to functional capacity were also analyzed. In the case of the patients who were not submitted to kinesiotherapy, there were reductions in the levels of IL-5, IL-6 and IL-10, and an increase in the number of CD11b + leukocytes, in addition to an increase in abdominal circumference after the monitoring time. Patients submitted to kinesiotherapy did not present changes in serum cytokines or in the numbers of CD11b + and CXCR2 + neutrophils and lymphocytes, but there were increases of flexibility and strength, as well as a reduction in pain sensation after the monitoring time. In conclusion, kinesiotherapy was able to increase flexibility and reduce pain in SLE patients without influencing immune parameters.

Intraoral diode laser epiglottectomy for treatment of epiglottis chondrosarcoma in a dog.

Laryngeal tumours are rare in dogs. Surgery is the treatment of choice, but it is usually palliative in malignant conditions, due to advanced stage of the tumour at the time of diagnosis. In veterinary medicine, little information is available about the use of diode laser in laryngeal oncological surgery. In the case reported here, a dog with an epiglottic chondrosarcoma was successfully treated with diode laser epiglottectomy. The surgical technique and follow up are described.

Immunohistochemical detection of P-glycoprotein (clone C494) in canine mammary gland tumours.

Elevated levels of P-glycoprotein have been reported in multidrug-resistant tumours in both humans and dogs. In the present study, we investigated the expression of P-glycoprotein in 57 canine mammary gland tumours, 10 mammary gland hyperplasia and seven normal mammary glands by immunohistochemistry. Tissue sections were incubated with an anti-Pgp monoclonal antibody and visualized with En Vision-DAB polymer. Normal and hyperplastic mammary tissues were negative or showed slight cytoplasmic immunoreactivity. Neoplastic cells in benign mammary tumours showed diffuse cytoplasmic staining, in contrast to malignant tumours that showed mainly a membranous staining pattern for Pgp (C494). We observed statistically significant differences among all the different groups of tissues analysed except for benign tumours versus hyperplasia (P = 0.221). Receiver-operating characteristic analysis showed that the best cut-off point to differentiate the threshold to differentiate negative from positive tissue samples was 18.40% of immunostained cells. These results provide a first indication that routine evaluation of Pgp expression in canine mammary gland tumours, taking into consideration a cut-off point for positivity, may be useful for selecting cases for chemotherapy.

Alteracoes osseas associadas a traumas musculares periodicos / Changes in bone associated to periodic muscle trauma

O objetivo do prosente trabalho foi avaliar a resposta do tecido osseo a traumas musculares periodicos. O trama muscular foi produzido de forma nao-invasiva por um equipamento que libera carga sobre a superficie muscular. Os animais foram divididos aleatoriamente em 2 grupos experimentais: o primeiro foi subemtido a 4 traumas periodicos e avaliacao 7 dias apos (n=5) e o grupo controle nao foi traumatizado (n=5).O trauma foi aplicado na regiao anterior do ventre do musculo tibialis anterior (TA) direito. apos os traumas, alem das tibias direita e esquerda, os musculos TAs direito e esquerdo de ambos os grupos foram analizados. A pata contralateral dos animais do grupo traumatizado foi mantida intacta. apos o sacrificio dos animais, as tibias forma submetidas as seguintes analises. resistencia ossea, peso das cinzas, volume osseo e densidade mineral ossea. as tibias traumatizadas periodicamente apresenta maiores valores de forca maxima ate ate o ponto de fratura ossea quando comparadas as contralaterais (71,78+14,32 N vs. 49,19+17,51 N, respectivamente, p,< 0,03),indicando aumento da resistencia ossea. A analise dos parametro fisicos mostrou qu o peso das cinzas das tibias traumatizadas foi maior que o dascontralaterais intactas (0,313+0,01 vs. 0,276+0,03 g, respectivamente, p= 0,002),tambem quando comprados ao grupo controle (0,313+0,01 g vs. 0,24+0,02 g, respectivamente, p=0,003) No entanto, nao foiram observadas alteracoes nos valores do volume osseo e da densidade mineral ossea entre as tibias traumatizadas e controlaterais do grupo traumatizado periodicamente e entre as tibias de ambos os grupos experimentais. conclusoes. Ccontusoes muscular periodicas promovem alteracoes significativas no conteudo mineral osseo e na resistencia ossea, sem causar mudancas na densidade mineral ossea