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1.
Oncogene ; 32(39): 4675-82, 2013 Sep 26.
Artículo en Inglés | MEDLINE | ID: mdl-23085758

RESUMEN

Biallelic protein-truncating mutations in the adenomatous polyposis coli (APC) gene are prevalent in sporadic colorectal cancer (CRC). Mutations may not be fully inactivating, instead producing WNT/ß-catenin signalling levels 'just-right' for tumourigenesis. However, the spectrum of optimal APC genotypes accounting for both hits, and the influence of clinicopathological features on genotype selection remain undefined. We analysed 630 sporadic CRCs for APC mutations and loss of heterozygosity (LOH) using sequencing and single-nucleotide polymorphism microarrays, respectively. Truncating APC mutations and/or LOH were detected in 75% of CRCs. Most truncating mutations occurred within a mutation cluster region (MCR; codons 1282-1581) leaving 1-3 intact 20 amino-acid repeats (20AARs) and abolishing all Ser-Ala-Met-Pro (SAMP) repeats. Cancers commonly had one MCR mutation plus either LOH or another mutation 5' to the MCR. LOH was associated with mutations leaving 1 intact 20AAR. MCR mutations leaving 1 vs 2-3 intact 20AARs were associated with 5' mutations disrupting or leaving intact the armadillo-repeat domain, respectively. Cancers with three hits had an over-representation of mutations upstream of codon 184, in the alternatively spliced region of exon 9, and 3' to the MCR. Microsatellite unstable cancers showed hyper-mutation at MCR mono- and di-nucleotide repeats, leaving 2-3 intact 20AARs. Proximal and distal cancers exhibited different preferred APC genotypes, leaving a total of 2 or 3 and 0 to 2 intact 20AARs, respectively. In conclusion, APC genotypes in sporadic CRCs demonstrate 'fine-tuned' interdependence of hits by type and location, consistent with selection for particular residual levels of WNT/ß-catenin signalling, with different 'optimal' thresholds for proximal and distal cancers.


Asunto(s)
Poliposis Adenomatosa del Colon/genética , Transformación Celular Neoplásica/genética , Neoplasias Colorrectales/genética , Genes APC , Vía de Señalización Wnt , Adulto , Anciano , Anciano de 80 o más Años , Codón/genética , Neoplasias del Colon/genética , Neoplasias del Colon/patología , Neoplasias Colorrectales/patología , Femenino , Genotipo , Humanos , Pérdida de Heterocigocidad , Masculino , Inestabilidad de Microsatélites , Persona de Mediana Edad , Mutación , Especificidad de Órganos , Neoplasias del Recto/genética , Neoplasias del Recto/patología , Eliminación de Secuencia , Neoplasias del Colon Sigmoide/genética , Neoplasias del Colon Sigmoide/patología , Vía de Señalización Wnt/genética
2.
Oncogene ; 27(44): 5808-20, 2008 Oct 02.
Artículo en Inglés | MEDLINE | ID: mdl-18591934

RESUMEN

The adenomatous polyposis coli (APC) tumour suppressor is a multifunctional protein involved in the regulation of Wnt signalling and cytoskeletal dynamics. Little is known about how APC controls these disparate functions. In this study, we have used APC- and axin-fluorescent fusion proteins to examine the interactions between these proteins and show that the functionally distinct populations of APC are also spatially separate. Axin-RFP forms cytoplasmic punctate structures, similar to endogenous axin puncta. Axin-RFP recruits beta-catenin destruction complex proteins, including APC, beta-catenin, glycogen synthase kinase-3-beta (GSK3-beta) and casein kinase-1-alpha (CK1-alpha). Recruitment into axin-RFP puncta sequesters APC from clusters at cell extensions and this prevents its microtubule-associated functions. The interaction between APC-GFP and axin-RFP within the cytoplasmic puncta is direct and dramatically alters the dynamic properties of APC-GFP. However, recruitment of APC to axin puncta is not absolutely required for beta-catenin degradation. Instead, formation of axin puncta, mediated by the DIX domain, is required for beta-catenin degradation. An axinDeltaDIX mutant did not form puncta, but still mediated recruitment of destruction complex proteins and phosphorylation of beta-catenin. We conclude that there are distinct pools of APC and that the formation of axin puncta, rather than the axin/APC complex, is essential for beta-catenin destruction.


Asunto(s)
Proteína de la Poliposis Adenomatosa del Colon/metabolismo , Proteínas Represoras/metabolismo , beta Catenina/metabolismo , Proteína de la Poliposis Adenomatosa del Colon/genética , Animales , Proteína Axina , Línea Celular , Citoplasma/metabolismo , Citoplasma/ultraestructura , Perros , Transferencia Resonante de Energía de Fluorescencia , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Ratones , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Proteínas Represoras/genética
3.
Biochem Pharmacol ; 71(10): 1422-34, 2006 May 14.
Artículo en Inglés | MEDLINE | ID: mdl-16522318

RESUMEN

The tyrphostin 4-(3-chloroanilino)-6,7-dimethoxyquinazoline (AG1478) is a potent and specific inhibitor of EGFR tyrosine kinase whose favourable preclinical profile supports progression towards clinical trials. Microphysiometric evaluation revealed a short (<24 min) effective inhibition of cellular receptor response to EGF challenge in BaF/ERX cells indicating a need to maintain sustained levels of inhibitor. Initial pharmacokinetic evaluation in mice of novel AG1478 formulations in a beta-cyclodextrin (Captisol) showed monoexponential elimination from plasma (half-life 30 min) following subcutaneous administration. A two-fold dose escalation gave a 2.4-fold increase in the total AUC. Bolus i.v. and 6 h continuous infusion were investigated in rats to mimic a more clinically relevant administration regimen. Drug elimination following bolus i.v. administration was biphasic (terminal elimination half-life 30-48 min). The linear relationship between dose and AUC(0-->infinity) (r2=0.979) enabled the prediction of infusion rates and doses for sustained delivery using continuous 6 h infusions, where steady state was reached in 120 min. Plasma levels of AG1478>10 microM were achieved over the duration of the infusion. At the lowest dose, plasma drug levels after the cessation of infusion declined with a half-life of approximately 43 min. EGFR activity, measured both by autophosphorylation and downstream signalling, was inhibited in a dose-dependent manner by injection of AG1478 in mice bearing xenografts of the human glioblastoma cell line U87MG.delta2-7, which expresses a constitutively active variant of the EGF receptor. Taken together, these experiments provide essential data to assess the anti-tumour efficacy of AG1478 and will assist in the rational design of dose regimens for clinical studies.


Asunto(s)
Inhibidores Enzimáticos/farmacocinética , Receptores ErbB/antagonistas & inhibidores , Proteínas Tirosina Fosfatasas/antagonistas & inhibidores , Tirfostinos/farmacocinética , Animales , Área Bajo la Curva , Línea Celular Tumoral , Relación Dosis-Respuesta a Droga , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/farmacología , Glioblastoma/tratamiento farmacológico , Glioblastoma/metabolismo , Glioblastoma/patología , Humanos , Inyecciones Intravenosas , Inyecciones Subcutáneas , Ratones , Estructura Molecular , Quinazolinas , Ratas , Timidina/metabolismo , Tirfostinos/química , Tirfostinos/farmacología , Ensayos Antitumor por Modelo de Xenoinjerto
4.
J Proteome Res ; 4(5): 1646-56, 2005.
Artículo en Inglés | MEDLINE | ID: mdl-16212417

RESUMEN

A biosensor-based micro-affinity purification method to recover protein binding partners and their complexes for down stream proteomics analysis has been developed using the BIAcore 3000 fitted with a prototype Surface Prep Unit (SPU). The recombinant GST-intracellular domain of E-cadherin or the recombinant GST-beta-catenin binding domain of Adenomatous Polyposis Coli (APC) were immobilized onto the SPU and used to affinity purify binding partners from chromatographically enriched SW480 colon cancer cell lysates. A GST- immobilized surface was used as a control. Samples recovered from the SPU were subjected to SDS-PAGE with sensitive Coomassie staining followed by automated in-gel digestion and LC-MS/MS. The results obtained using the SPU were compared with similar experiments performed using Sepharose beads.


Asunto(s)
Técnicas Biosensibles , Proteómica/métodos , Western Blotting , Cadherinas/química , Línea Celular Tumoral , Cromatografía , Cromatografía por Intercambio Iónico , Cromatografía Liquida , Electroforesis en Gel de Poliacrilamida , Glutatión Transferasa/metabolismo , Humanos , Espectrometría de Masas , Péptidos/química , Fosforilación , Unión Proteica , Estructura Terciaria de Proteína , Proteínas/química , Proteínas Recombinantes/química , Sefarosa/farmacología , Factores de Tiempo , beta Catenina/química
5.
Br J Cancer ; 92(6): 1069-77, 2005 Mar 28.
Artículo en Inglés | MEDLINE | ID: mdl-15770208

RESUMEN

We report the generation of a chimeric monoclonal antibody (ch806) with specificity for an epitope on the epidermal growth factor receptor (EGFR) that is different from that targeted by all other anti-EGFR therapies. Ch806 antibody is reactive to both de2-7 and overexpressed wild-type (wt) EGFR but not native EGFR expressed in normal tissues at physiological levels. Ch806 was stably expressed in CHO (DHFR -/-) cells and purified for subsequent characterisation and validated for use in preliminary immunotherapy investigations. Ch806 retained the antigen binding specificity and affinity of the murine parental antibody. Furthermore, ch806 displayed enhanced antibody-dependent cellular cytotoxicity against target cells expressing the 806 antigen in the presence of human effector cells. Ch806 was successfully radiolabelled with both iodine-125 and indium-111 without loss of antigen binding affinity or specificity. The radioimmunoconjugates were stable in the presence of human serum at 37 degrees C for up to 9 days and displayed a terminal half-life (T(1/2beta)) of approximately 78 h in nude mice. Biodistribution studies undertaken in BALB/c nude mice bearing de2-7 EGFR-expressing or amplified EGFR-expressing xenografts revealed that (125)I-labelled ch806 failed to display any significant tumour retention. However, specific and prolonged tumour localisation of (111)In-labelled ch806 was demonstrated with uptake of 31%ID g(-1) and a tumour to blood ratio of 5 : 1 observed at 7 days postinjection. In vivo therapy studies with ch806 demonstrated significant antitumour effects on established de2-7 EGFR xenografts in BALB/c nude mice compared to control, and both murine 806 and the anti-EGFR 528 antibodies. These results support a potential therapeutic role of ch806 in the treatment of suitable EGFR-expressing tumours, and warrants further investigation of the potential of ch806 as a therapeutic agent.


Asunto(s)
Anticuerpos Monoclonales/uso terapéutico , Receptores ErbB/inmunología , Neoplasias Experimentales/terapia , Proteínas Recombinantes de Fusión/uso terapéutico , Animales , Anticuerpos Monoclonales/farmacocinética , Células CHO , Cricetinae , Femenino , Humanos , Inmunoterapia , Ratones , Ratones Endogámicos BALB C , Trasplante de Neoplasias , Ingeniería de Proteínas , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Recombinantes de Fusión/farmacocinética , Distribución Tisular , Trasplante Heterólogo
6.
Growth Factors ; 20(2): 71-80, 2002 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-12148565

RESUMEN

Epidermal Growth Factor (EGF) is a small growth factor containing 53 amino acid residues capable of stimulating the proliferation of both mesenchymal and epithelial cells. Comparison of the amino acid sequences of EGF from several species, and related proteins that can bind to the EGF receptor (e.g. TGFalpha, VGF, heparin-binding EGF, and betacellulin), suggests that Leu47, which is highly conserved, is important for biological function. Additionally, we have shown previously, using a combination of trypsin and carboxypeptidase Y digestion of native murine EGF, that removal of Leu47 results in more than 100-fold decrease in both receptor binding and mitogenic activity. We now describe a micromethod for the rapid generation of C-terminally modified EGFs to investigate further the role of C-terminal residues in determining functional activity. These analogues have been generated by digesting native murine EGF with trypsin, purifying the biologically inactive, but structurally intact, EGF1-45 core by micropreparative RP-HPLC, and then reversing the action of trypsin to couple synthetic peptides (e.g. DL, DI, DF, EL, DLLW) onto the C-terminus of the EGF1-45 core. This enzymic semisynthesis method allows multiple derivatives to be generated rapidly from microgram quantities of EGF1-45 in sufficient quantities for sensitive biological and physicochemical analysis. We have validated the method by regenerating EGF1-47 from EGF1-45 with equivalent mitogenic and receptor binding activity to EGF1-47 generated from wild type EGF by digestion with trypsin and carboxypeptidase Y. We have also investigated the effect of substituting alternative normal or nonphysiological amino acids (e.g. allo-Ile) for Asp46, Leu47 or Arg48. Even small changes in these C-terminal residues reduce the mitogenic potency of the analogue.


Asunto(s)
Factor de Crecimiento Epidérmico/análogos & derivados , Factor de Crecimiento Epidérmico/síntesis química , Células 3T3 , Secuencia de Aminoácidos , Aminoácidos/química , Animales , Carboxipeptidasas/farmacología , Catepsina A , Cromatografía Líquida de Alta Presión , Relación Dosis-Respuesta a Droga , Humanos , Cinética , Leucina/química , Ligandos , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Péptidos/química , Unión Proteica , Estructura Terciaria de Proteína , Homología de Secuencia de Aminoácido , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Relación Estructura-Actividad , Factores de Tiempo , Tripsina/farmacología
7.
Mol Cell Biol Res Commun ; 4(6): 321-30, 2001 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-11703090

RESUMEN

Members of transforming growth factor beta (TGF-beta) family are potent regulators of multiple cellular functions, including cell proliferation, differentiation, migration, organization, and death. Yet the signaling pathways underpinning a wide array of biological activities of TGF-beta appear to be deceptively simple. At every step from TGF-beta secretion to activation of its target genes, the activity of TGF-beta is regulated tightly, both positively and negatively. Biologically active TGF-beta is cleaved from a precursor protein (latent form) and multiple process factors control the levels of active TGF-beta. The efficient secretion, correct folding and deposition to the extracellular matrices require the cosecretion of latent TGF-beta binding proteins (LTBPs). Once activated, TGF-beta ligand signals through a heteromeric receptor complex of two distinct type I and type II serine/threonine kinase receptors TbetaRI and TbetaRII. Many factors appear to influence the formation of the active ligand-receptor complex. The relative orientation of TbetaRI and TbetaRII in the ligand-receptor complex is critical for activation: through TbetaRI, the activated ligand-receptor complex directly binds and phosphorylates downstream intracellular substrates, called Smads. Inhibitory Smads, Smad6 and 7, can antagonize this process. The phosphorylation of Smads leads to the formation of complexes which translocate to the nucleus. Other signaling systems can modulate the activity of the Smads: e.g., ras activity can prevent Smad complexes from entering the nucleus and specific ubiquitin ligases can target Smad for degradation. In the nucleus, the Smad complexes associate with other transcription activators or suppressors to regulate gene expression, either positively or negatively. The combined effects of the positive and/or negative TGF-beta controlled gene expression together with the endogenous protein set of the target cell are responsible for the multiplicity of biological functions.


Asunto(s)
Transducción de Señal , Factor de Crecimiento Transformador beta/metabolismo , Factor de Crecimiento Transformador beta/fisiología , Transporte Activo de Núcleo Celular , Animales , Proteínas de Unión al ADN/metabolismo , Regulación de la Expresión Génica , Humanos , Ligandos , Modelos Biológicos , Unión Proteica , Proteína smad6 , Proteína smad7 , Transactivadores/metabolismo , Transcripción Genética
8.
J Clin Oncol ; 19(19): 3976-87, 2001 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-11579119

RESUMEN

PURPOSE: KM871 is a chimeric monoclonal antibody against the ganglioside antigen GD3, which is highly expressed on melanoma cells. We conducted an open-label, dose escalation phase I trial of KM871 in patients with metastatic melanoma. PATIENTS AND METHODS: Seventeen patients were entered onto one of five dose levels (1, 5, 10, 20, and 40 mg/m2). Patients received three infusions of KM871 at 2-week intervals, with the first infusion of KM871 trace-labeled with indium-111 (111In) to enable assessment of biodistribution in vivo. Biopsies of metastatic melanoma sites were performed on days 7 to 10. RESULTS: Fifteen of 17 patients completed a cycle of three infusions of KM871. No dose-limiting toxicity was observed during the trial; the maximum-tolerated dose was therefore not reached. Three patients (at the 1-, 5-, and 40-mg/m2 dose levels) developed pain and/or erythema at tumor sites consistent with an inflammatory response. No normal tissue uptake of 111In-KM871 was observed, and tumor uptake of 111In-KM871 was observed in all lesions greater than 1.5 cm (tumor biopsy 111KM871 uptake results: range, 0.001% to 0.026% injected dose/g). The ratio of maximum tumor to normal tissue was 15:1. Pharmacokinetic analysis revealed a 111In-KM871 terminal half-life of 7.68 +/- 2.94 days. One patient had a clinical partial response that lasted 11 months. There was no serologic evidence of human antichimeric antibody in any patient, including one patient who received 16 infusions over a 12-month period. CONCLUSION: This study is the first to demonstrate the biodistribution and specific targeting of an anti-GD3 antibody to metastatic melanoma in patients. The long half-life and lack of immunogenicity of KM871 makes this antibody an attractive potential therapy for patients with metastatic melanoma.


Asunto(s)
Anticuerpos Monoclonales/inmunología , Anticuerpos Monoclonales/farmacocinética , Complejo CD3/inmunología , Melanoma/inmunología , Melanoma/metabolismo , Adulto , Anciano , Anticuerpos Monoclonales/efectos adversos , Anticuerpos Monoclonales/uso terapéutico , Especificidad de Anticuerpos , Biopsia , Femenino , Humanos , Inmunoconjugados/efectos adversos , Inmunoconjugados/inmunología , Inmunoconjugados/farmacocinética , Radioisótopos de Indio , Masculino , Melanoma/diagnóstico por imagen , Melanoma/terapia , Persona de Mediana Edad , Cintigrafía , Proteínas Recombinantes de Fusión/efectos adversos , Proteínas Recombinantes de Fusión/inmunología , Proteínas Recombinantes de Fusión/farmacocinética , Proteínas Recombinantes de Fusión/uso terapéutico , Distribución Tisular
9.
J Neurosurg ; 95(3): 472-9, 2001 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-11565870

RESUMEN

OBJECT: Activation of signaling by the epidermal growth factor receptor (EGFR) through gene amplification or rearrangement is common in human malignancy, especially in a large fraction of de novo glioblastomas multiforme (GBMs). The most common mutant EGFR, (AEGFR, also known as de2-7 EGFR and EGFRvIII) lacks a portion of the extracellular domain, enhances tumorigenicity in vivo, and causes resistance to the chemotherapeutic drug cisplatin (CDDP). This resistance is due to the suppression of CDDP-induced apoptosis by the constitutively active tyrosine kinase activity of the receptor. The authors have investigated whether inhibition of AEGFR signaling by the tyrosine kinase inhibitor, tyrphostin AG1478, could sensitize tumor xenografts to CDDP and, thereby, enhance its therapeutic efficacy in animals. METHODS: Nude mice were inoculated either subcutaneously or intracerebrally with human GBM cells expressing AEGFR and were then systemically treated with CDDP and/or AG1478. Tumor volumes were monitored and tumor sections were analyzed by using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assays or MIB-1 staining. Expression of AEGFR, but not wild-type EGFR, conferred CDDP resistance to the cells in vivo. Inhibition of receptor signaling by the EGFR-specific tyrosine kinase inhibitor, AG1478. sensitized the xenografts to the cytotoxic effects of CDDP. This combined CDDP/AG1478 treatment significantly suppressed growth of subcutaneous xenografts in nude mice in a synergistic manner (p < 0.01 compared with vehicle control) without causing generalized toxicity, whereas treatments with CDDP or AG1478 alone were ineffective. The synergistic growth suppression by the CDDP/AG1478 combination was not observed in xenografts overexpressing wild-type EGFR or kinase-deficient AEGFR. The combined CDDP/ AG1478 treatment induced tumor growth suppression, which correlated with increased apoptosis and reduced proliferation. This treatment also extended the life span of mice bearing intracerebral xenografts (p < 0.01 compared with controls). CONCLUSIONS: The results of this study may provide the basis for the development of a novel and safe therapeutic strategy for the very aggressive AEGFR-expressing GBM.


Asunto(s)
Neoplasias Encefálicas/genética , Supervivencia Celular/genética , Cisplatino/farmacología , Inhibidores Enzimáticos/farmacología , Receptores ErbB/genética , Glioblastoma/genética , Mutación/genética , Proteínas Tirosina Fosfatasas/antagonistas & inhibidores , Tirfostinos/farmacología , Animales , Neoplasias Encefálicas/patología , Supervivencia Celular/efectos de los fármacos , Receptores ErbB/efectos de los fármacos , Femenino , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Glioblastoma/patología , Humanos , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Trasplante de Neoplasias , Quinazolinas , Trasplante Heterólogo , Células Tumorales Cultivadas
10.
J Pept Res ; 58(3): 204-12, 2001 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-11576326

RESUMEN

Large numbers of colon tumors stem from mutations in the gene coding for the production of the adenomatous polyposis coli (APC) tumor suppressor protein. This protein contains a coiled-coil N-terminal domain that is known to be responsible for homodimerization. Previous work by others has led to the design of a specific 54-residue anti-APC peptide (anti-APCp1) that dimerizes preferentially with this domain. We have undertaken the chemical synthesis of a modified form of this peptide (anti-APCp2) that bears a biotin moiety at its N-terminus for use in subsequent ligand-binding analysis studies. The peptide was subjected to comprehensive chemical characterization to confirm its purity. Secondary structural analysis by circular dichroism spectroscopy and Fourier transform infrared spectroscopy indicated that the peptide could assume a wide range of potential conformations, depending upon the precise microenvironment. Significantly, a stable alpha-helical structure was generated when the solvent conditions supported intramolecular salt-bridge formation along the helix barrel. The biotinylated anti-APCp2 was immobilized onto a streptavidin sensor surface, in a specific orientation leaving all amino acids available to form a coiled structure. In one experiment, injection of colonic cell lysate extracts (LIM1215) onto a size-exclusion column resulted in the isolation of a high molecular mass protein peak (> 600 kDa) that reacted specifically with the immobilized anti-APCp2 on the biosensor surface. In another experiment, a high molecular mass protein (M(r) > 250 kDa on SDS-PAGE) could be specifically immunoprecipitated from this peak using either the anti-APCp2 peptide or an anti-APC polyclonal antibody. This demonstrates the specific interaction between the anti-APCp2 peptide and native APC and highlights the potential use of the former peptide in a multidimensional micropreparative chromatographic/biosensor/proteomic protocol for the purification of APC alone and APC complexed with different biopolymers in various cell lines, and stages of tumor development.


Asunto(s)
Proteína de la Poliposis Adenomatosa del Colon/aislamiento & purificación , Poliposis Adenomatosa del Colon/metabolismo , Técnicas Biosensibles/métodos , Neoplasias del Colon/química , Sondas Moleculares/química , Péptidos/química , Proteína de la Poliposis Adenomatosa del Colon/química , Secuencia de Aminoácidos , Cromatografía Líquida de Alta Presión , Dicroismo Circular , Neoplasias del Colon/terapia , Humanos , Péptidos/análisis , Pruebas de Precipitina/métodos , Estructura Secundaria de Proteína , Soluciones/química , Espectroscopía Infrarroja por Transformada de Fourier , Células Tumorales Cultivadas , Agua/química
11.
Biochemistry ; 40(30): 8930-9, 2001 Jul 31.
Artículo en Inglés | MEDLINE | ID: mdl-11467954

RESUMEN

Murine and human epidermal growth factor receptors (EGFRs) bind human EGF (hEGF), mouse EGF (mEGF), and human transforming growth factor alpha (hTGF-alpha) with high affinity despite the significant differences in the amino acid sequences of the ligands and the receptors. In contrast, the chicken EGFR can discriminate between mEGF (and hEGF) and hTGF-alpha and binds the EGFs with approximately 100-fold lower affinity. The regions responsible for this poor binding are known to be Arg(45) in hEGF and the L2 domain in the chicken EGFR. In this study we have produced a truncated form of the hEGFR ectodomain comprising residues 1-501 (sEGFR501), which, unlike the full-length hEGFR ectodomain (residues 1-621, sEGFR621), binds hEGF and hTGF-alpha with high affinity (K(D) = 13-21 and 35-40 nM, respectively). sEGFR501 was a competitive inhibitor of EGF-stimulated mitogenesis, being almost 10-fold more effective than the full-length EGFR ectodomain and three times more potent than the neutralizing anti-EGFR monoclonal antibody Mab528. Analytical ultracentrifugation showed that the primary EGF binding sites on sEGFR501 were saturated at an equimolar ratio of ligand and receptor, leading to the formation of a 2:2 EGF:sEGFR501 dimer complex. We have used sEGFR501 to generate three mutants with single position substitutions at Glu(367), Gly(441), or Glu(472) to Lys, the residue found in the corresponding positions in the chicken EGFR. All three mutants bound hTGF-alpha and were recognized by Mab528. However, mutant Gly(441)Lys showed markedly reduced binding to hEGF, implicating Gly(441), in the L2 domain, as part of the binding site that recognizes Arg(45) of hEGF.


Asunto(s)
Factor de Crecimiento Epidérmico/metabolismo , Receptores ErbB/genética , Receptores ErbB/metabolismo , Fragmentos de Péptidos/genética , Fragmentos de Péptidos/metabolismo , Eliminación de Secuencia , Animales , Unión Competitiva/genética , Técnicas Biosensibles , Células CHO , Línea Celular , Pollos , Cricetinae , Dimerización , Factor de Crecimiento Epidérmico/antagonistas & inhibidores , Receptores ErbB/biosíntesis , Receptores ErbB/aislamiento & purificación , Inhibidores de Crecimiento/genética , Inhibidores de Crecimiento/metabolismo , Humanos , Ligandos , Ratones , Mutagénesis Sitio-Dirigida , Fragmentos de Péptidos/biosíntesis , Fragmentos de Péptidos/aislamiento & purificación , Plásmidos/biosíntesis , Plásmidos/metabolismo , Unión Proteica/genética , Estructura Terciaria de Proteína/genética , Transfección , Factor de Crecimiento Transformador alfa/metabolismo
12.
J Pept Res ; 58(1): 67-78, 2001 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-11454171

RESUMEN

Acetyl thioalanine N-methyl (Ac-Alat-NHMe) and thioacetyl alanine N-methyl (Act-Ala-NHMe) were synthesized, crystallized and their X-ray diffraction structures determined for the first time. Both molecules adopted beta-sheet conformations and showed similar hydrogen bonding patterns with one molecular surface forming two oxo hydrogen bonds and the other forming two thio hydrogen bonds. The crystal structure data for the two thioamides provided a validation of the thioamide parameters for the newly derived CFF91 force field because the observed crystal (phi, psi) angles were situated in the global minimum regions of the theoretical (phi, psi) map predicted using the parameters. In addition, the parameters were further validated because conformational energy minimization of the crystal structure produced low deviations in unit cell dimensions, bond lengths, bond angles and torsional angles, and a 120-ps molecular dynamics simulation also gave a low deviation for the most probable N-H...S=C bond distance.


Asunto(s)
Alanina/química , Alanina/análogos & derivados , Bioquímica/métodos , Cristalografía por Rayos X , Enlace de Hidrógeno , Modelos Moleculares , Compuestos de Azufre/química , Tioamidas/química
13.
Cancer Res ; 61(14): 5355-61, 2001 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-11454674

RESUMEN

The monoclonal antibody (mAb) 806 was raised against the delta2-7 epidermal growth factor receptor (de2-7 EGFR or EGFRvIII), a truncated version of the EGFR commonly expressed in glioma. Unexpectedly, mAb 806 also bound the EGFR expressed by cells exhibiting amplification of the EGFR gene but not to cells or normal tissue expressing the wild-type receptor in the absence of gene amplification. The unique specificity of mAb 806 offers an advantage over current EGFR antibodies, which all display significant binding to the liver and skin in humans. Therefore, we examined the antitumor activity of mAb 806 against human tumor xenografts grown in nude mice. The growth of U87 MG xenografts, a glioma cell line that endogenously expresses approximately 10(5) EGFRs in the absence of gene amplification, was not inhibited by mAb 806. In contrast, mAb 806 significantly inhibited the growth of U87 MG xenografts transfected with the de2-7 EGFR in a dose-dependent manner using both preventative and established tumor models. Significantly, U87 MG cells transfected with the wild-type EGFR, which increased expression to approximately 10(6) EGFRs/cell and mimics the situation of gene amplification, were also inhibited by mAb 806 when grown as xenografts in nude mice. Xenografts treated with mAb 806 all displayed large areas of necrosis that were absent in control tumors. This reduced xenograft viability was not mediated by receptor down-regulation or clonal selection because levels of antigen expression were similar in control and treated groups. The antitumor effect of mAb 806 was not restricted to U87 MG cells because the antibody inhibited the growth of new and established A431 xenografts, a cell line expressing >10(6) EGFRs/cell. This study demonstrates that mAb 806 possesses significant antitumor activity.


Asunto(s)
Anticuerpos Monoclonales/farmacología , Antineoplásicos/farmacología , Receptores ErbB/genética , Neoplasias Experimentales/tratamiento farmacológico , Animales , Anticuerpos Monoclonales/metabolismo , División Celular/efectos de los fármacos , División Celular/genética , Receptores ErbB/inmunología , Femenino , Regulación Neoplásica de la Expresión Génica , Genotipo , Humanos , Inmunohistoquímica , Ratones , Mutación , Neoplasias Experimentales/genética , Neoplasias Experimentales/patología , Pruebas de Precipitina , Unión Proteica , Trasplante Heterólogo , Resultado del Tratamiento , Células Tumorales Cultivadas/metabolismo , Ensayos Antitumor por Modelo de Xenoinjerto
14.
Cancer Res ; 61(11): 4474-82, 2001 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-11389078

RESUMEN

The chimeric monoclonal antibody KM871, directed against the G(D3) antigen, is under evaluation for its potential to target melanoma. To facilitate the in vivo evaluation of biodistribution properties and measurement of pharmacokinetics, KM871 was radiolabeled with (125)I via tyrosine residues and with (111)In via the bifunctional metal ion chelator C-functionalized trans-cyclohexyl diethylenetriaminepentaacetic acid (CHX-A"-DTPA) to lysine residues. Using antigen-positive SK-MEL-28 melanoma cells, immunoreactivities of 42 and 40% cell binding were obtained, respectively, for the two radioconjugates. Binding was enhanced in the presence of added unlabeled antibody. A humanized A33 antibody was similarly labeled with the two isotopes and used as a control. To determine and compare in vivo biodistribution characteristics of KM871 radiolabeled with (111)In or (125)I, mixtures of the radioconjugates were injected i.v. into BALB/c nude mice bearing G(D3)-positive-SK-MEL-28 melanoma xenografts. Gamma camera images were acquired; groups of five mice were sacrificed at various time intervals, and tumors, blood, and tissues were analyzed. (111)In-labeled CHX-A"-DTPA-KM871 showed a maximum tumor uptake of 41.9 +/- 7.0% injected dose/g at 72 h with prolonged retention over a 15-day period. The tumor:blood ratio was 3:1 by 72 h, and higher ratios were observed at later time points. No abnormal accumulation of (111)In-labeled conjugate was found in normal tissues. In contrast, there was little accumulation of (125)I-labeled KM871 in the same tumors. The specificity of antibody localization was confirmed by the low tumor uptake values for radiolabeled control antibody. Gamma camera imaging demonstrated excellent uptake of (111)In-labeled CHX-A"-DTPA-KM871 in the xenografts. Chromatographic analyses of xenograft cytosolic extracts demonstrated tumor internalization and catabolism of radiolabeled KM871 with the formation of small molecular weight metabolites. Laser scanning confocal microscopy demonstrated that the majority of intracellular KM871 is localized to lysosomes. Despite the catabolism of the radioconjugate, a dose-dependent increase in KM871 tumor localization was shown through immunohistochemical examination of xenograft biopsies. This study demonstrates for the first time the in vivo localization of a radiolabeled anti-G(D3) monoclonal antibody to G(D3)-expressing xenografts using gamma camera scanning techniques and tumor cell internalization of KM871 tagged with a green fluorescent dye, Alexa Fluor 488, through confocal microscopy. KM871 has potential for targeting tumors in patients with melanoma.


Asunto(s)
Anticuerpos Monoclonales/farmacocinética , Gangliósidos/inmunología , Inmunoconjugados/farmacocinética , Melanoma/diagnóstico por imagen , Melanoma/metabolismo , Ácido Pentético/análogos & derivados , Radiofármacos/farmacocinética , Animales , Anticuerpos Monoclonales/inmunología , Quelantes/química , Quelantes/farmacocinética , Femenino , Cámaras gamma , Gangliósidos/biosíntesis , Humanos , Inmunohistoquímica , Radioisótopos de Indio/química , Radioisótopos de Yodo/química , Isotiocianatos/química , Isotiocianatos/farmacocinética , Marcaje Isotópico , Melanoma/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Ácido Pentético/química , Ácido Pentético/farmacocinética , Cintigrafía , Proteínas Recombinantes de Fusión/inmunología , Proteínas Recombinantes de Fusión/farmacocinética , Distribución Tisular , Células Tumorales Cultivadas , Ensayos Antitumor por Modelo de Xenoinjerto
15.
Exp Cell Res ; 266(1): 1-10, 2001 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-11339819

RESUMEN

Signals from the epidermal growth factor (EGF) receptor and integrin-dependent adhesion to laminin contribute to the progression and metastasis of colonic tumors. However, little is know about the mechanisms by which these signals cooperate. Recently, we have reported that the colon cancer cell line LIM1215 secretes and adhere to autocrine laminin-10 via multiple integrin receptors and that EGF stimulates spreading of these cells on the same substrate. In this report, we investigate the effect of EGF and laminin-10 on colon cancer cell migration in vitro. EGF stimulates migration of LIM1215 cells in a wound healing assay. The response to EGF is inhibited by anti-EGF receptor antibody 528, the EGF receptor kinase inhibitor AG-1478, or the MAP kinase kinase inhibitor PD98059 but not the PI3-K inhibitor wortmannin. Using Transwell migration chambers, we demonstrate that laminin-10 but not collagen-I, collagen-IV, or a commercial preparation of human placental laminin is a potent motility factor for LIM1215 cells. The migration response to laminin-10 is increased upon stimulation of the cells with EGF and correlates with the up-regulation of alpha(6)beta(4) integrin expression as measured by analysis of Triton X-100-soluble cellular extracts. The results from integrin inhibition experiments indicate that basal migration on laminin-10 is mediated by alpha(3)beta(1) but not alpha(2)beta(1) nor alpha(6)beta(4) integrins. Alpha(3) blocking antibodies also inhibited EGF-stimulated chemokinetic migration of LIM1215 cells on laminin-10. However, in contrast to unstimulated cells, alpha(6) or beta(4) integrin-blocking antibodies inhibited the migration of EGF-stimulated cells by up to 50%. Taken together, these results support the cooperative role of EGF receptor and laminin-10 on colon cancer cell motility and suggest a critical role for both the alpha(3)beta(1) and the alpha(6)beta(4) integrins in this process.


Asunto(s)
Antígenos de Superficie/metabolismo , Carcinoma/metabolismo , Movimiento Celular/fisiología , Neoplasias del Colon/metabolismo , Factor de Crecimiento Epidérmico/metabolismo , Integrinas/metabolismo , Laminina/metabolismo , Laminina/farmacología , Metástasis de la Neoplasia/fisiopatología , Células Tumorales Cultivadas/metabolismo , Actinas/efectos de los fármacos , Actinas/metabolismo , Antígenos de Superficie/efectos de los fármacos , Bioensayo , Carcinoma/fisiopatología , Movimiento Celular/efectos de los fármacos , Neoplasias del Colon/fisiopatología , Citoesqueleto/efectos de los fármacos , Citoesqueleto/metabolismo , Factor de Crecimiento Epidérmico/farmacología , Humanos , Integrina alfa3beta1 , Integrina alfa6beta4 , Integrinas/efectos de los fármacos , Factores de Tiempo , Células Tumorales Cultivadas/efectos de los fármacos
16.
Protein Eng ; 14(1): 39-45, 2001 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-11287677

RESUMEN

Drugs that inhibit important protein-protein interactions are hard to find either by screening or rational design, at least so far. Most drugs on the market that target proteins today are therefore aimed at well-defined binding pockets in proteins. While computer-aided design is widely used to facilitate the drug discovery process for binding pockets, its application to the design of inhibitors that target the protein surface initially seems to be limited because of the increased complexity of the task. Previously, we had started to develop a computational combinatorial design approach based on the well-known 'multiple copy simultaneous search' (MCSS) procedure to tackle this problem. In order to identify sequence patterns of potential inhibitor peptides, a three-step procedure is employed: first, using MCSS, the locations of specific functional groups on the protein surface are identified; second, after constructing the peptide main chain based on the location of favorite locations of N-methylacetamide groups, functional groups corresponding to amino acid side chains are selected and connected to the main chain C(alpha) atoms; finally, the peptides generated in the second step are aligned and probabilities of amino acids at each position are calculated from the alignment scheme. Sequence patterns of potential inhibitors are determined based on the propensities of amino acids at each C(alpha) position. Here we report the optimization of inhibitor peptides using the sequence patterns determined by our method. Several short peptides derived from our prediction inhibit the Ras--Raf association in vitro in ELISA competition assays, radioassays and biosensor-based assays, demonstrating the feasibility of our approach. Consequently, our method provides an important step towards the development of novel anti-Ras agents and the structure-based design of inhibitors of protein--protein interactions.


Asunto(s)
Técnicas Químicas Combinatorias , Péptidos/química , Proteínas Proto-Oncogénicas c-raf/antagonistas & inhibidores , Proteínas ras/antagonistas & inhibidores , Algoritmos , Secuencia de Aminoácidos , Técnicas Biosensibles , Diseño Asistido por Computadora , Ensayo de Inmunoadsorción Enzimática , Humanos , Modelos Moleculares , Biblioteca de Péptidos , Péptidos/farmacología , Inhibidores de Proteasas/síntesis química , Unión Proteica , Estructura Secundaria de Proteína , Proteínas Proto-Oncogénicas c-raf/metabolismo , Ensayo de Unión Radioligante , Alineación de Secuencia , Proteínas ras/metabolismo
17.
J Obstet Gynecol Neonatal Nurs ; 30(2): 139-47, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11308103

RESUMEN

From statutory rape, pregnancy options, mandatory reporting, and emancipation, a wide range of medicolegal issues face teenagers. These issues become even more complex when the teenager is pregnant. Nurses caring for pregnant and parenting teenagers are in a position to offer advocacy and support in family planning, prenatal, obstetric, and pediatrics settings. A comprehensive understanding of common medicolegal issues facing teenagers will help to ensure appropriate patient advocacy.


Asunto(s)
Enfermería Maternoinfantil/legislación & jurisprudencia , Enfermería Maternoinfantil/métodos , Defensa del Paciente/legislación & jurisprudencia , Embarazo en Adolescencia , Violación/legislación & jurisprudencia , Maltrato Conyugal/legislación & jurisprudencia , Adolescente , Servicios de Planificación Familiar/organización & administración , Femenino , Humanos , Relaciones Enfermero-Paciente , Pennsylvania , Embarazo , Embarazo en Adolescencia/psicología , Embarazo en Adolescencia/estadística & datos numéricos , Violación/prevención & control , Violación/psicología , Violación/estadística & datos numéricos , Apoyo Social , Maltrato Conyugal/prevención & control , Maltrato Conyugal/psicología , Maltrato Conyugal/estadística & datos numéricos , Estados Unidos
18.
J Chromatogr B Biomed Sci Appl ; 754(1): 193-9, 2001 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-11318415

RESUMEN

The tyrphostin 4-(3-chloroanilino)-6,7-dimethoxyquinazoline (AG1478) is undergoing evaluation as a potential new anticancer agent. We have developed a specific and sensitive reversed-phase HPLC assay for AG1478 in mouse plasma. The method involves a rapid and simple extraction process followed by separation on a Symmetry C8 stationary phase with a gradient of acetonitrile in ammonium acetate buffer. A linear response was achieved over the concentration range of 0.2-100 microM using multilevel calibration with internal standard method of calculation. Inter- and intra-assay accuracy and precision were better than +/-10%. The limit of quantitation was 0.2 microM. We have used this method to study the preclinical pharmacokinetics of this new agent in mice.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Receptores ErbB/antagonistas & inhibidores , Tirfostinos/sangre , Animales , Inhibidores Enzimáticos/sangre , Estabilidad de Enzimas , Congelación , Ratones , Quinazolinas , Estándares de Referencia , Sensibilidad y Especificidad , Tirfostinos/farmacocinética
19.
J Mol Graph Model ; 20(3): 245-56, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11766049

RESUMEN

When the oxygen atom in a peptide bond is replaced by a sulfur atom, the restriction in the available conformational space and the ability of thioamides to confer resistance to enzymatic degradation renders thioamides as potentially useful building blocks for drug design and protein engineering. The solvation free energy differences between conformers of the same dipeptide can be high. Yet, previous conformational studies, basing on the (phi, psi) conformational energy maps of thio-substituted dipeptides, neglected both explicit water interactions and free energy considerations. In this paper, the (phi, psi) conformational free energy maps are obtained by single umbrella sampling in an explicit water environment for both alanine dipeptide and the corresponding thioamide derivatives. The phi and psi angles for the minima in the relative energy maps calculated with dielectric of 80 are similar to the corresponding phi and psi angles in the relative free energy maps for both Ac-Ala-NHMe (Ac: acetyl; Ala: alanine) and Act-Alat-NHMe (Act: thio-acetyl; Alat: thio-alanine). However, some large differences between the relative energy and relative free energy of major minima indicate that the consideration of free energy is important in determination of the relative occupancy of particular minima. Free energy maps for both Ac-Ala-NHMe and Act-Alat-NHMe show that thio-substitution favors conformations where phi < 0 because of the deeper beta and alphaR minima. The changes in the position and relative stability of minima were explained in terms of the destabilization of the regions near phi = -120, 0 and 120, psi = 60, -60, 180, which correspond to the increased steric hindrance due to the bulkier sulfur atom.


Asunto(s)
Alanina/química , Antibacterianos/química , Dipéptidos/química , Péptidos , Péptidos Catiónicos Antimicrobianos , Simulación por Computador , Transferencia de Energía , Cómputos Matemáticos , Conformación Proteica
20.
Issues Ment Health Nurs ; 22(5): 549-69, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11881515

RESUMEN

In the emerging health care environment, families and communities are facing a broad range of conditions that require more integrated views of wellness and illness, mind and body, and the biomedical and the behavioral. This also calls for an enhanced care delivery system. Three interfacing areas of practice have particular significance for the psychiatric-mental health nursing workforce: enhanced primary care, public health or population-focused health interventions, and managed care. This paper addresses the broad range of knowledge, skills, and competencies that are required for the future of advanced practice psychiatric-mental health nursing and describes interactive learning experiences and new pedagogies to deliver them.


Asunto(s)
Educación en Enfermería/tendencias , Programas Controlados de Atención en Salud/tendencias , Enfermeras Clínicas/educación , Enfermería Psiquiátrica/educación , Curriculum/tendencias , Predicción , Humanos , Estados Unidos
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