RESUMEN
Burkholderia pseudomallei is a causative agent of melioidosis. The present study investigated IL-1beta mRNA and protein expression by peripheral blood mononuclear cells and purified monocytes (n = 10) in response to infection with B. pseudomallei and B. thailandensis. Similarly increased IL-1beta mRNA and protein expression was found in both PBMC and purified monocytes stimulated with B. pseudomallei and B. thailandensis. Thus, this study suggests that IL-1beta response does not differ between infections with B. pseudomallei and its non-virulent counterpart and other mechanisms may be involved in their distinct virulence in causing the disease.
Asunto(s)
Burkholderia pseudomallei/inmunología , Burkholderia/inmunología , Interleucina-1beta/biosíntesis , Leucocitos Mononucleares/inmunología , Monocitos/inmunología , Burkholderia pseudomallei/patogenicidad , Células Cultivadas , Humanos , Leucocitos Mononucleares/metabolismo , Leucocitos Mononucleares/microbiología , Monocitos/metabolismo , Monocitos/microbiología , ARN Mensajero/metabolismoRESUMEN
Burkholderia pseudomallei is the causative agent of melioidosis. One of the main risk factors for B. pseudomallei infection in endemic areas is diabetes mellitus. The present study investigated IL-17 mRNA and protein expression by peripheral blood mononuclear cells in response to B. pseudomallei infection in 10 diabetic patients in comparison to 10 healthy blood donors. The IL-17 expression in diabetic patients was significantly lower (p < 0.05) than in the controls. However, IL-23 mRNA expression of the 2 groups was comparable. The present findings suggest that melioidosis affects T cell IL-17 production and that patients with diabetes mellitus have a defective IL-17 production in response to this type of infection.
Asunto(s)
Burkholderia pseudomallei/inmunología , Diabetes Mellitus Tipo 1/inmunología , Diabetes Mellitus Tipo 2/inmunología , Interleucina-17/sangre , Leucocitos Mononucleares/inmunología , Melioidosis/inmunología , Adulto , Humanos , Interleucina-17/genética , Interleucina-23/sangre , Leucocitos Mononucleares/metabolismo , Leucocitos Mononucleares/microbiología , Melioidosis/complicaciones , Melioidosis/metabolismo , Melioidosis/microbiología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Linfocitos T/inmunologíaRESUMEN
PROBLEM: As there has been a study in mice showing the expression of IL-17 by decidual cells and the status of IL-17 receptor expression in human pregnancy is not known, we hypothesized that IL-17 may regulate human trophoblast proliferation and invasion. METHOD OF STUDY: JEG-3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL-17 receptor protein and mRNA, respectively. The effects of IL-17 on JEG-3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively. RESULTS: IL-17 increased the invasive capacity of JEG-3 cells but had no effect on the proliferation and multinucleated formation of JEG-3 cells. CONCLUSION: In this JEG-3 cell model of human trophoblast, the IL-17R and IL-17 may have a regulatory role in trophoblast invasion.