RESUMEN
Oral squamous cell carcinoma (OSCC) is a worldwide public health problem, with high morbidity and mortality rates. The development of new drugs to treat OSCC is paramount. Piper plant species have shown many biological activities. In the present study, we show that dichloromethane partition of Piper cernuum (PCLd) is nontoxic in chronic treatment in mice, reduces the amount of atypia in tongues of chemically induced OSCC, and significantly increases animal survival. To identify the main active compounds, chromatographic purification of PCLd was performed, where fractions 09.07 and 14.05 were the most active and selective. These fractions promoted cell death by apoptosis characterized by phosphatidyl serine exposition, DNA fragmentation, and activation of effector caspase-3/7 and were nonhemolytic. LC-DAD-MS/MS analysis did not propose matching spectra for the 09.07 fraction, suggesting compounds not yet known. However, aporphine alkaloids were annotated in fraction 14.05, which are being described for the first time in P. cernuum and corroborate the observed cytotoxic activity. Putative molecular targets were determined for these alkaloids, in silico, where the androgen receptor (AR), CHK1, CK2, DYRK1A, EHMT2, LXRß, and VEGFR2 were the most relevant. The results obtained from P. cernuum fractions point to promising compounds as new preclinical anticancer candidates.
RESUMEN
Objetivo: relatar caso clínico de tumor odontogênico adenomatoide (TOA), enfatizando suas características e diagnósticos diferenciais. Relato de caso: paciente masculino, 16 anos, apresentando lesão radiolúcida, unilocular, associada ao dente 24 incluso. Sob as hipóteses de TOA, cisto dentígero, tumor odontogênico cístico calcificante e ameloblastoma unicístico, foi realizada biópsia excisional. Na microscopia, observou-se parênquima contendo estruturas tubulares e áreas semelhantes à roseta e cribriformes, estabelecendo o diagnóstico de TOA. Conclusão: o reconhecimento das características clínicas, radiográficas e histopatológicas do TOA é essencial para guiar o processo para se estabelecer o diagnóstico definitivo e, consequentemente, permitir o tratamento adequado do paciente.
Objective: to report a case of adenomatoid odontogenic tumor (AOT), highlighting its features and differential diagnosis. Case report: a 16-year-old male with a unilocular radiolucent lesion involving the unerupted 24th tooth. Considering AOT, dentigerous cyst, calcifying cystic odontogenic tumor, and unicystic ameloblastoma as the clinical diagnoses, an excisional biopsy was performed. Microscopic analysis revealed a parenchyma containing tubular structures and roset-like areas, establishing a diagnosis of AOT. Conclusion: the acknowledgement of clinical, radiographic, and microscopic features of AOT is essential to guide a definitive diagnosis and, as a consequence, to enable a suitable treatment of the patient.