In vitro and in vivo evaluation of the efficacies of commercial probiotics and disinfectant against acute hepatopancreatic necrosis disease and luminescent vibriosis in Litopenaeus vannamei.

The bioactivities of two commercially available probiotics and one chemical disinfectant were tested against strains of Vibrio parahaemolyticus (VPAHPND) and V. harveyi. This study aimed to determine shrimp pathogenic Vibrios' in vitro and in vivo sensitivities to commercial probiotics and a chemical disinfectant. The probiotics and disinfectant were tested first in vitro, followed by the in vivo trials. Results showed that upon administration of probiotics either through diet or adding into the tank water, the survivability of shrimp was increased during challenge with VPAHPND and V. harveyi. Also, the disinfectant was tested against the same pathogens and showed positive bactericidal effects at 2500 ppm and 5000 ppm. The present findings suggest that adding probiotics to the rearing water or the shrimp feeds effectively prevents infection by lowering the load of pathogenic bacteria. In comparison, the effectiveness of the disinfectant (PUR) depends on its appropriate concentration and timing of application. It is not only limited to rearing water but is also applicable for decontaminating pond liners, tanks, and other paraphernalia.

Exogenous sulphide donors modify the gene expression patterns of Atlantic salmon nasal leukocytes.

Hydrogen sulphide (H2S) is a known mediator of immunity, but the regulatory function of its exogenous form is not well understood in fish particularly in the mucosa. Here we report transcriptomic changes in the nasal leukocytes of Atlantic salmon (Salmo salar) following exposure to two forms of H2S donors - the salt sodium hydrosulfide (NaHS) and the organic analogue morpholin-4-ium 4-methoxyphenyl (morpholino) phosphinodithioate (GYY4137). Nasal leukocytes were exposed to three concentrations (1, 10 and 100 µM) of either of the two H2S forms for 24 h before the cells were checked for viability and collected for microarray analysis. Though cellular viability was minimally affected by the exposure to two H2S donors, GYY4137-exposed cells exhibited reduced viability compared with the NaHS group at the highest dose. The H2S-induced transcriptomic changes in the nasal leukocytes were concentration-dependent regardless of the sulphide forms. However, a larger number of differentially expressed genes (DEGs) were identified in the NaHS-exposed versus GYY4137-exposed groups across concentrations. In all comparisons, at least 53% of the DEGs identified were significantly upregulated. Gene ontology (GO) terms enriched in the lists of upregulated DEGs at higher concentrations included ferric iron binding. A comparison of the two H2S forms showed a clear grouping of different GO terms relative to concentrations. Pathway enrichment analysis revealed a significant influence in VEGF ligand-receptor interactions, oxidative stress, innate and adaptive immunity, and interleukin signalling especially at higher concentrations. Congruence analysis demonstrated that there were 16 GO terms overlapping; of these, 12 were upregulated by both sulphide donors including several involving iron binding and transport. The study offers the first molecular insights into how fish nasal leukocytes respond to exogenous H2S, and the results will be vital in resolving the regulatory function of H2S on mucosal immunity in fish.

Draft Genome Sequence of Vibrio parahaemolyticus Strain PH1339, Which Causes Acute Hepatopancreatic Necrosis Disease in Shrimp in the Philippines.

We report the first draft genome sequence of an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strain isolated from a Penaeus vannamei sample from the Philippines. The strain carries the genes encoding the Pir-like toxin pair PirAvp and PirBvp.

Draft Genome Sequence of Multidrug-Resistant Vibrio parahaemolyticus Strain PH698, Infecting Penaeid Shrimp in the Philippines.

The emergence of multidrug-resistant bacterial strains in diverse settings has been reported globally. In the Philippine shrimp aquaculture industry, antibiotics are used for the treatment of bacterial diseases during the production cycle. We report the draft genome of Vibrio parahaemolyticus PH698, a multidrug-resistant strain isolated from a Philippine shrimp farm.

Acute hepatopancreatic necrosis disease (AHPND) outbreaks in Penaeus vannamei and P. monodon cultured in the Philippines.

Acute hepatopancreatic necrosis disease (AHPND) has recently emerged as a serious disease of cultured shrimp. It has also been described as early mortality syndrome (EMS) due to mass mortalities occurring within 20 to 30 d after stocking of ponds with postlarvae. Here, Penaeus vannamei and Penaeus monodon from shrimp farms in the Philippines were examined for the toxin-producing strain of Vibrio parahaemolyticus due to AHPND-like symptoms occurring in marketable size shrimp. In the P. vannamei, histology revealed typical AHPND pathology, such as sloughing of undifferentiated cells in the hepatopancreatic tubule epithelium. Analysis using the IQ2000 AHPND/EMS Toxin 1 PCR test generated 218 bp and 432 bp amplicons confirmative of the toxin-producing strain of V. parahaemolyticus among shrimp sampled from 8 of 9 ponds. In the P. monodon, histology revealed massive sloughing of undifferentiated cells of the hepatopancreatic tubule epithelium in the absence of basophilic bacterial cells. PCR testing generated the 2 amplicons confirmatory for AHPND among shrimp sampled from 5 of 7 ponds. This study confirms the presence of AHPND in P. vannamei and P. monodon farmed in the Philippines and suggests that the disease can also impact late-stage juvenile shrimp.