RESUMEN
Elevated levels of reactive oxygen species (ROS) have demonstrated efficacy in eliminating tumor cells by modifying the tumor microenvironment and inducing the polarization of tumor-associated macrophages (TAMs). Nevertheless, the transient nature and limited diffusion distance inherent in ROS present significant challenges in cancer treatment. In response to these limitations, we have developed a nanoparticle (MnClPc-HSA@GOx) that not only inhibits tumor energy metabolism but also facilitates the transition of TAMs from the M2 type (anti-inflammatory type) to the M1 type (proinflammatory type). MnClPc-HSA@GOx comprises a manganese phthalocyanine complex (MnClPc) enveloped in human serum albumin (HSA), with glucose oxidase (GOx) loaded onto MnClPc@HSA nanoparticles. GOx was employed to catalyze the decomposition of glucose to produce H2O2 and gluconic acid. Additionally, in the presence of MnClPc, it catalyzes the conversion of H2O2 into â¢O2- and 1O2. Results indicate that the nanoparticle effectively impedes the glucose supply to tumor cells and suppresses their energy metabolism. Simultaneously, the ROS-mediated polarization of TAMs induces a shift from M2 to M1 macrophages, resulting in a potent inhibitory effect on tumors. This dual-action strategy holds promising clinical inhibition applications in the treatment of cancer.
Asunto(s)
Isoindoles , Nanopartículas , Neoplasias , Humanos , Manganeso/farmacología , Glucosa Oxidasa/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Peróxido de Hidrógeno/metabolismo , Neoplasias/metabolismo , Macrófagos , Oxígeno/metabolismo , Metabolismo Energético , Glucosa , Microambiente TumoralRESUMEN
Varicose veins are among the most common disorders of the vascular system; however, the pathogenesis of varicose veins remains unclear. The present study aimed to investigate the roles of microRNA (miR)199a5p in varicose veins and in the phenotypic transition of vascular smooth muscle cells (VSMCs). Bioinformatics analysis confirmed that miR199a5p had target sites on the forkhead box C2 (FOXC2) 3'untranslated region. Reverse transcriptionquantitative PCR (RTqPCR) and western blotting were used to detect the expression levels of miR199a5p and FOXC2 in varicose vein and normal great saphenous vein tissues. Cell Counting Kit8 and Transwell migration assays were performed to validate the effects of miR199a5p on VSMCs. Contractile markers, such as smooth muscle 22α, calponin, smooth muscle actin and myosin heavy chain 11 were used to detect phenotypic transition. RTqPCR revealed that miR199a5p was downregulated in varicose veins compared with expression in normal great saphenous veins, whereas FOXC2 was upregulated in varicose veins. In addition, biomarkers of the VSMC contractile phenotype were downregulated in varicose veins. Overexpression of miR199a5p by mimics suppressed VSMC proliferation and migration, whereas depletion of miR199a5p enhanced VSMC proliferation and migration. Notably, the effects caused by miR199a5p could be reversed by FOXC2 overexpression. Dual luciferase reporter analysis confirmed that FOXC2 was a target of miR199a5p. In conclusion, miR199a5p may be a novel regulator of phenotypic switching in VSMCs by targeting FOXC2 during varicose vein formation.