Influence of genetic polymorphisms on oral health-related quality of life after root canal treatment.

To evaluate the impact of genetic polymorphisms in interleukins (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), nitric oxide (NOS2 rs2779249, rs2897518) and suppressor of cytokine signaling (SOCS1 rs243327, rs33977706) on oral health-related quality of life (OHRQoL) of patients under-going root canal treatment (RCT). METHODS: The sample consisted of 108 participants, presenting single-rooted teeth with asymptomatic periapical periodontitis. The impact of the OHRQoL was recorded using the Oral Health Impact Profile (OHIP-14) before, seven, and 30 days after RCT. Saliva samples were collected as a source of genomic DNA. Genetic polymorphisms were genotyped by Real-Time PCR using the Taqman method. Univariate and Multivariate analyses were used (p<0.05). RESULTS: A significant difference was observed for the polymorphism rs2297518 in the NOS2 gene in functional limitation in the codominant (p=0.037) and recessive (p=0.001) models; in the physical pain (p<0.001 in both models); in psychological discomfort (p<0.001 in both models); in physical disability (p<0.001 in both models) and in psychological disability (p<0.001 in both models). Polymorphisms in the SOCS1 gene, in the recessive model, rs33977706 (p=0.045) and rs243327 (p=0.019), influenced the OHRQoL in the psychological discomfort domain. CONCLUSIONS: Polymorphisms in NOS2 and SOCS1 genes influenced the OHRQoL of patients undergoing RCT.

Influence of genetic polymorphisms on oral health-related quality of life after root canal treatment

Abstract To evaluate the impact of genetic polymorphisms in interleukins (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), nitric oxide (NOS2 rs2779249, rs2897518) and suppressor of cytokine signaling (SOCS1 rs243327, rs33977706) on oral health-related quality of life (OHRQoL) of patients under-going root canal treatment (RCT). Methods: The sample consisted of 108 participants, presenting single-rooted teeth with asymptomatic periapical periodontitis. The impact of the OHRQoL was recorded using the Oral Health Impact Profile (OHIP-14) before, seven, and 30 days after RCT. Saliva samples were collected as a source of genomic DNA. Genetic polymorphisms were genotyped by Real-Time PCR using the Taqman method. Univariate and Multivariate analyses were used (p<0.05). Results: A significant difference was observed for the polymorphism rs2297518 in the NOS2 gene in functional limitation in the codominant (p=0.037) and recessive (p=0.001) models; in the physical pain (p<0.001 in both models); in psychological discomfort (p<0.001 in both models); in physical disability (p<0.001 in both models) and in psychological disability (p<0.001 in both models). Polymorphisms in the SOCS1 gene, in the recessive model, rs33977706 (p=0.045) and rs243327 (p=0.019), influenced the OHRQoL in the psychological discomfort domain. Conclusions: Polymorphisms in NOS2 and SOCS1 genes influenced the OHRQoL of patients undergoing RCT.

Resumo Avaliar o impacto de polimorfismos genéticos em interleucinas (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), óxido nítrico (NOS2 rs2779249, rs2897518) e supressor da sinalização de citocinas (SOCS1 rs243327, rs33977706) na qualidade de vida relacionada à saúde bucal (QVRSB) de pacientes submetidos a tratamento endodôntico (TE). Métodos: A amostra foi composta por 108 participantes, que apresentavam dentes unirradiculares com lesão periapical assintomática. O impacto da QVRSB foi registrado usando o Oral Health Impact Profile (OHIP-14) antes, sete e 30 dias após o TE. Amostras de saliva foram coletadas como fonte de DNA genômico. Os polimorfismos genéticos foram genotipados por PCR em tempo real usando o método Taqman. Análises univariadas e multivariadas foram utilizadas (p<0,05). Resultados: Observou-se diferença significativa para o polimorfismo rs2297518 no gene NOS2 na limitação funcional nos modelos codominante (p=0,037) e recessivo (p=0,001); na dor física (p<0,001 em ambos os modelos); no desconforto psicológico (p<0,001 em ambos os modelos); na deficiência física (p<0,001 em ambos os modelos) e na deficiência psicológica (p<0,001 em ambos os modelos). Polimorfismos no gene SOCS1, no modelo recessivo, rs33977706 (p=0,045) e rs243327 (p=0,019), influenciaram a QVRSB no domínio desconforto psicológico. Conclusões: Polimorfismos nos genes NOS2 e SOCS1 influenciaram a QVRSB de pacientes submetidos a TE.

Genetic polymorphisms in TNF-α as a potential biomarker for oral health-related quality of life in children.

This cross-sectional study aimed to assess if genetic polymorphisms in TNF- α are associated with a negative impact on Oral Health-Related Quality of Life (OHRQoL) in children with dental caries. A total of 307 pairs of parents/caregivers and children aged two to five years were selected. The children were clinically evaluated and classified according to caries experience and severity of active caries. The Brazilian Portuguese version of the Early Childhood Oral Health Impact Scale (ECOHIS) was used to assess OHRQoL. Genotyping analysis of genetic polymorphisms in TNF- α (rs1799724, rs1799964, and rs1800629) was performed using real-time polymerase chain reaction. In the recessive model, children with the CC genotype of TNF-α (rs1799964) had a significantly high chance of poor OHRQoL in the symptom domain (pain), in both the caries experience (p = 0.045) and the high-severity active caries phenotypes (p = 0.033) (Mann-Whitney U test). It was concluded that genetic polymorphisms in TNF-α are associated with OHRQoL related to the symptom domain (pain), suggesting that TNF-α could be used as a potential biomarker for OHRQoL. Understanding the genetic aspects associated with OHRQoL will allow the early identification of patients with OHRQoL disparities and provide personalized healthcare.

Evidence of Association between MTRR and TNF-α Gene Polymorphisms and Oral Health-Related Quality of Life in Children with Anterior Open Bite.

Genetic polymorphisms could explain the inter-individual differences in the oral health-related quality of life (OHRQoL) of children with anterior open bite (AOB). OBJECTIVE: To assess the impact of AOB on OHRQoL in children and to evaluate whether MTR (rs1805087), MTRR (rs1801394), TGFß1 (rs1800469) and TNF-α (rs1799964, rs1799724 and rs1800629) genes are potential biomarkers for OHRQoL in children with AOB. STUDY DESIGN: A cross-sectional study was performed with 173 children aged between 2-6 years. The Brazilian version of Early Childhood Oral Health Impact Scale (ECOHIS) was applied. Genetic polymorphisms were analyzed using real-time PCR. Mann-Whitney U-test and Chi-square were used. RESULTS: The overall mean ECOHIS scores were 5.49 (SD= 5.72) and 3.45 (SD = 4.49) (p < 0.01) in the AOB and control groups, respectively. Children with the CC genotype of TNF-α (rs1799724) had a significantly higher psychological QoL level. The MTRR AA genotype group showed a lower QoL level in the child subscale (p = 0.006), function (p = 0.017), and psychological (p = 0.006) domains. There was no significant difference between OHRQoL and the genetic polymorphisms in MTR and TGFß1. CONCLUSIONS: Genetic polymorphisms in TNF-α and MTRR are associated with the impact on the OHRQoL in children with AOB.

Genetic polymorphisms in TNF-α as a potential biomarker for oral health-related quality of life in children

Abstract: This cross-sectional study aimed to assess if genetic polymorphisms in TNF- α are associated with a negative impact on Oral Health-Related Quality of Life (OHRQoL) in children with dental caries. A total of 307 pairs of parents/caregivers and children aged two to five years were selected. The children were clinically evaluated and classified according to caries experience and severity of active caries. The Brazilian Portuguese version of the Early Childhood Oral Health Impact Scale (ECOHIS) was used to assess OHRQoL. Genotyping analysis of genetic polymorphisms in TNF- α (rs1799724, rs1799964, and rs1800629) was performed using real-time polymerase chain reaction. In the recessive model, children with the CC genotype of TNF-α (rs1799964) had a significantly high chance of poor OHRQoL in the symptom domain (pain), in both the caries experience (p = 0.045) and the high-severity active caries phenotypes (p = 0.033) (Mann-Whitney U test). It was concluded that genetic polymorphisms in TNF-α are associated with OHRQoL related to the symptom domain (pain), suggesting that TNF-α could be used as a potential biomarker for OHRQoL. Understanding the genetic aspects associated with OHRQoL will allow the early identification of patients with OHRQoL disparities and provide personalized healthcare.

Interleukin 1 alpha genetic polymorphisms as potential biomarkers for oral health-related quality of life in Para athletes.

AIMS: To assess the impact of dental caries on OHRQoL in Para athletes and to evaluate whether interleukin 1 alpha (IL1A) (rs17561, rs1304037), interleukin 10 (IL10) (rs1800871), and interleukin 1 receptor antagonist (IL1RN) (rs9005) genes are potential biomarkers for OHRQoL in Para athletes. MATERIALS AND METHODS: A cross-sectional study consisting of 264 Para athletes (athletics, 143; powerlifting, 61; and swimming, 60) aged between 14 and 79 years was conducted. The decayed-missing-filled teeth index was used for the clinical evaluation. The Brazilian version of the Oral Health Impact Profile (OHIP-14) was used to measure the OHRQoL. Genomic DNA was extracted from the saliva. Genetic polymorphisms were analyzed by real-time polymerase chain reaction. Descriptive and bivariate analyses were performed. RESULTS: The overall mean OHIP-14 score observed was 6.24 (standard deviation, 7.05) and 10.03 (standard deviation, 8.11) in Para athletes with no caries experience and with caries experience, respectively (p = .002). Para athletes with the A allele in the IL1A gene (rs17561), in a dominant model, had a significantly higher risk of poor psychological discomfort than those with the other allele (p = .03). CONCLUSION: Dental caries affected the OHRQoL in Para athletes. IL1A genetic polymorphisms were the potential biomarkers for OHRQoL in Para athletes.

Impact of traumatic dental injury treatment on the Oral Health-Related Quality of Life of children, adolescents, and their family: Systematic review and meta-analysis.

BACKGROUND/AIM: Measures of oral health-related quality of life (OHRQoL) are useful in clinical trials to evaluate treatment outcomes. To detect the treatment impact of traumatic dental injuries (TDI) on OHRQoL is extremely relevant. The aim of this study was to assess whether there is scientific evidence of the impact of TDI treatment on OHRQoL of children, adolescents, and their families. MATERIAL AND METHODS: A systematic review and meta-analysis were conducted selecting articles from PubMed, Web of Science, Scopus, Cochrane Library, and Virtual Health Library until May 22nd, 2020. The gray literature, clinical trial registers, and a manual search were performed. Two independent reviewers selected the studies, extracted the data, and assessed the ROBINS-I risk of bias. A meta-analysis was performed using RevMan 5.4 program. The certainty of the evidence was evaluated using the GRADE system. RESULTS: In the electronic search, 413 abstracts and one registered study were found. After removing the duplicates and eligibility application, six studies were included. Individually, the studies presented low to moderate risk of bias and they found that TDI treatment reduces the negative impact on OHRQoL. In the meta-analysis, TDI treatment improved OHRQoL for 8- to 10-year-old children (p = .03; CI 5.19 [0.62, 9.75]) (detected by CPQ 8-10 questionnaire). In the perception of parents, the children, and adolescents who had suffered TDI also had their OHRQoL improved (p = .04; CI 14.77 [0.95, 28.60]) (detected by PPQ and P-CPQ questionnaire) but both evaluations had very low certainty of evidence. CONCLUSION: Treatment of traumatic dental injuries reduces the impact on the OHRQoL of children and adolescents. However, more studies are necessary to detect the TDI treatment influence on OHRQoL of preschoolers and in the family as well to estimate the effects due to outcomes that had a very low certainty of evidence (#CRD42018091210).

Oral health outcomes: the association of clinical and socio-dental indicators to evaluate traumatic dental injury profile in low income Brazilian children.

OBJECTIVE: To use clinical and socio-dental indicators to evaluate traumatic dental injury (TDI) profile in Brazilian preschool children with low income. METHODS: A cross-sectional study was carried out with children of low-income families, from 2 to 6 year-old, who attended public preschools. A clinical indicator to evaluate TDI and a socio-dental indicator to detect the impact on Oral Health-Related Quality of Life (OHRQoL) for preschoolers (Early Childhood Impact Scale - ECOHIS) were used. Impact means were obtained, and the chi-square test and odds ratio were used in order to evaluate the variables (P<0.05). RESULTS: Of the 606 children evaluated, the results were obtained from a final sample of 446 children. The prevalence of TDI was 17%. Children from 37 to 60 months (P=0.04; OR=0.59 95% CI 0.34-1.01) were associated with TDI. The mean impact on OHRQoL in the group with or without TDI was low without a statistical difference (P=0.97). CONCLUSION: Based on these findings, the association of clinical and socio-dental indicators showed low prevalence of TDI and the impact on OHRQoL was indifferent between groups with or without TDI.

Impact of complicated and uncomplicated traumatic dental injuries on oral health-related quality of life of preschoolers and their family.

The severity of a traumatic dental injury (TDI) can influence the prognosis of deciduous teeth and the formation of permanent successors. Consequently, it can have a negative influence on the daily lives of children and their parents. The present study aimed to evaluate the impact of complicated and uncomplicated TDI on the oral health-related quality of life (OHRQoL) of preschoolers and their families. A cross-sectional study was carried out according to the Strengthening the Reporting of Observational Studies in Epidemiology guidelines. After sample calculation and application of the eligibility criteria, 76 children from public preschools (aged 2 to 5 years) with TDI were selected during a period of 5 months. TDI was diagnosed using the Dental Trauma Index and classified according to the severity as complicated or uncomplicated. The Brazilian version of the Early Childhood Oral Health Impact Scale (ECOHIS) was used to determine OHRQoL. The Poisson regression model and Poisson regression model with robust estimates were calculated according to p-values <0.05. The complicated group and the uncomplicated group presented low average impact according to ECOHIS scores and there was no significant difference in the total scale, subscale, and domains (P>0.05) according to the type of TDI. Based on these findings, it can be concluded that severity of TDI did not influence the OHRQoL of preschoolers and their families.

Childhood bruxism: Related factors and impact on oral health-related quality of life.

This study aimed to assess childhood bruxism relating associated factors and the bruxism's impact on oral health-related quality of life (OHRQoL). A case-control study was performed with 3- to 6-year-old children obtained from public preschools in Brazil. The case and control groups had 21 and 40 children, respectively. Associations between bruxism and respiratory problems (p = 0.04, OR: 0.33, CI: 0.09 to 1.14), dental wear (p < 0.01, OR: 0.01, CI: 0.00 to 0.05), malocclusion (p < 0.01, OR: 0.06, CI: 0.01 to 0.35), and dental caries (p = 0.02, OR: 0.22, CI: 0.04 to 1.04) were observed. The OHRQoL overall mean score and subscales were relatively low independent of the evaluated group (p > 0.05). The association between presence and absence of impact with bruxism or other variables showed no statistical relationship (p > 0.05). It could be concluded that childhood bruxism is related to respiratory problems, dental wear, dental caries, and malocclusion. Despite being a topic that demands special care in dentistry, bruxism does not significantly affect the OHRQoL.

Perfil Epidemiológico dos Traumatismos Dentários em Crianças e Adolescentes no Brasil / Epidemiological Profile of Dental Trauma in Children and Adolescents in Brazil

O traumatismo dentário - TD é considerado, pela Organização Mundial de Saúde ? OMS um problema de saúde pública que apresenta alta prevalência e gera impacto negativo na qualidade de vida relacionada à saúde bucal de crianças/adolescentes e familiares com sequelas biológicas, socioeconômicas, psicológicas e comportamentais. Assim, o presente estudo objetivou realizar uma revisão critica da literatura sobre os estudos que avaliam o perfil epidemiológico do TD em crianças e adolescentes, mapeando a distribuição desses estudos no Brasil. A busca na literatura foi feita utilizando os descritores: traumatismos dentários, criança, adolescente, e epidemiologia, nas Bases Medline, Lilacs, SciELO, BBO e no Google Acadêmico. Os mesmos termos em inglês foram utilizados no Pubmed. De 248 artigos encontrados inicialmente, foram removidas as duplicatas e aplicados os critérios de inclusão e exclusão, selecionando-se 53 artigos. Observou-se que há muitos estudos sobre a prevalência dos TD desenvolvidos no Brasil durante os últimos anos. No entanto, os estudos estão concentrados em algumas regiões específicas como Rio Grande do Sul, Santa Catarina, Minas Gerais e São Paulo. A prevalência de TD em dentição decídua foi de 9,4% e 62,1%, e permanente variou de 8,0% a 58,6%. Os fatores como gênero, sobressaliência acentuada e cobertura labial inadequada foram frequentemente associados ao TD em crianças e adolescentes. Baseado nos levantamentos epidemiológicos dos artigos selecionados neste trabalho, conclui-se que, devido à concentração das pesquisas em alguns centros, não há um perfil representativo da população brasileira e os dados compilados dos estudos devem ser comparados com cautela, devido à grande diferença metodológica existente nos estudos.

Traumatic dental injury (TDI) is considered by the World Health Organization as a public health problem with high prevalence, which generates negative impact on oral health related to quality of life of children / adolescents and their families, causing biological, socioeconomic, psychological and behavioral sequelae. The present study aimed to conduct a critical review of the literature on studies assessing the epidemiological profile of the TDI in children and adolescents, mapping the distribution of these studies in Brazil. The search was performed using the descriptors ?dental trauma, children, adolescents, epidemiology and Brazil?, in the Medline, Lilacs, SCiELO, BBO, Google Scholar, and Pubmed. Initially, 248 articles were found. After removing the duplicates and applying the inclusion and exclusion criteria, 53 articles were selected. It has been observed that there are many studies of prevalence of TDI developed in recent years in Brazil. However, these studies are concentrated in some specific areas such as Rio Grande do Sul, Santa Catarina, Minas Gerais, and São Paulo. The prevalence of TDI in the primary dentition was 9.4% and 62.1%, ranging from 8.0% to 58.6% in the permanent dentition. Factors such as gender, increased overjet and inadequate lip coverage were often associated with TDI in children and adolescents. Based on the epidemiological surveys of the articles selected in this study, we concluded that there is no representative profile of the Brazilian population due to the concentration of research in some centers, thus data should be compared with caution due to the large methodological difference between the studies.