A Novel Poly(3-hexylthiophene) Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas.

Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research, its application is limited by substantial interference in complex brain environments while ensuring biosafety requirements. In this study, we introduced poly(3-hexylthiophene) (P3HT) and nitrogen-doped multiwalled carbon nanotubes (N-MWCNTs) to construct a composite membrane-modified carbon fiber microelectrode (CFME/P3HT-N-MWCNTs) for ascorbic acid (AA) detection. The microelectrode presented good linearity, selectivity, stability, antifouling, and biocompatibility and exhibited great performance for application in neuroelectrochemical sensing. Subsequently, we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells, ex vivo brain slices, and in vivo living rat brains and determined that glutamate can induce cell edema and AA release. We also found that glutamate activated the N-methyl-d-aspartic acid receptor, which enhanced Na+ and Cl- inflow to induce osmotic stress, resulting in cytotoxic edema and ultimately AA release. This study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the mechanism. Our work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals, understand the molecular basis of nervous system diseases, and discover certain biomarkers of brain diseases.

Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients.

Programmed cell death ligand 1 protein-positive (PD-L1+) exosomes have been found to be a potential biomarker for the diagnosis of non-small cell lung cancer (NSCLC). However, the development of highly sensitive detection technique for PD-L1+ exosomes is still a challenge in clinical applications. Herein, a sandwich electrochemical aptasensor based on ternary metal-metalloid palladium-copper-boron alloy microporous nanospheres (PdCuB MNs) and Au@CuCl2 nanowires (NWs) was designed for the detection of PD-L1+ exosomes. The excellent peroxidase-like catalytic activity of PdCuB MNs and the high conductivity of Au@CuCl2 NWs endow the fabricated aptasensor with intense electrochemical signal, thus enabling the detection of low abundance exosomes. The analytical results revealed that the aptasensor maintained favorable linearity over a wide concentration range of 6 orders of magnitude and reached a low detection limit of 36 particles/mL. The aptasensor is successfully applied to the analysis of complex serum samples and achieves the accurate identification of clinical NSCLC patients. Overall, the developed electrochemical aptasensor provides a powerful tool for early diagnosis of NSCLC.