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1.
Psychol Res Behav Manag ; 16: 4353-4365, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37908679

RESUMEN

Objective: Learner dependence on short videos has many pitfalls for learning outcomes, but the negative effects of excessive short video use have been little discussed in the learning psychology literature. Therefore, this study investigated the effects of excessive short video use on anxiety, depression, prospective memory, and academically delayed gratification (ADOG) in relation to online gaming-related behaviours, and explored the possible mechanisms by which excessive online gaming and short video use may lead to decreased ADOG, to expand our understanding of excessive short video use. Methods: Based on the whole class random sampling method, a questionnaire survey was conducted among college students in Northern Anhui, China from May 7 to July 27, 2022. The questionnaires included the Generalized Anxiety Disorder Scale (GAD-7), Patient Health Questionnaire Scale (PHQ-9), Prospective and Retrospective Memory (PRM) Questionnaire, and ADOG Scale. Results: A total of 1016 participants completed the survey. The study found that of all the internet behaviors, 20.8% of the college students mainly played online games, 43.9% mainly played short videos, and 35.3% conducted other online behaviors. When compared with other internet behaviors, online gaming and short video behaviors can cause more serious anxiety/depression and worse PRM and ADOG scores. As time spent playing online games and short videos increased, anxiety and depression became worse, and the scores for PRM and ADOG also declined. Anxiety, depression, and PRM mediate the relationship between time spent on online gaming/short videos and ADOG. Conclusion: Excessive short videos behaviour may produce the same psychological problems and learning problems as online gaming disorder. Excessive short video and online gaming behaviors may affect ADOG performance through anxiety, depression, and prospective memory. These findings could be used as a basis for future studies on the improvement of ADOG.

3.
Anal Chem ; 91(21): 14125-14132, 2019 11 05.
Artículo en Inglés | MEDLINE | ID: mdl-31583883

RESUMEN

Herein, an ultrasensitive electrochemiluminescent (ECL) strategy was proposed based on a highly efficient dynamic DNA machine based on microRNA triggered free movement on the lipid bilayer interface. Typically, the lipid bilayer is constructed on the electrode surface modified with nafion@ECL luminophore and gold nanoparticles to immobilize the DNA walker labeled with cholesterol and hairpin nucleotides labeled with cholesterol and ferrocene (Fc), based on the cholesterol-lipid interaction. On this state, Fc was close to the ECL luminophore, performing a quenched ECL emission. In the presence of target microRNA 21, it could trigger the entropy beacon-based DNA amplification to convert microRNA to massive special DNA sequences, which could further hybridize with the blocking DNA on DNA walker to reactivate the DNA walker and thus trigger the DNA walker-based amplification to make Fc to be far from the ECL luminophore, performing a recovered ECL emission related with the concentration of microRNA 21. Compared with the conventional DNA walker immobilized on the interface via chemical bonds or physical adsorption, a higher reaction efficiency could be achieved due to the free movements of DNA walker and its substrates on the interface. As expected, satisfactory performances for the detection of microRNA 21 were achieved with a detection limit of 0.4 fM and quantitative estimation in cells. Furthermore, this dynamic DNA machine-based ECL strategy could be readily expanded for the detection of other biomarkers for clinical diagnosis.


Asunto(s)
Técnicas Biosensibles , ADN/química , Técnicas Electroquímicas , Membrana Dobles de Lípidos/química , Mediciones Luminiscentes , MicroARNs/química , Termodinámica , Electrodos , Células HeLa , Humanos , Células MCF-7 , Técnicas de Amplificación de Ácido Nucleico , Propiedades de Superficie , Células Tumorales Cultivadas
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