Development of colonic stent simulator using three-dimensional printing technique: a simulator development study in Korea.

Background/Aims: Colonic stenting plays a vital role in the management of acute malignant colonic obstruction. The increasing use of self-expandable metal stents (SEMS) and the diverse challenges posed by colonic obstruction at various locations underscore the importance of effective training for colonic stent placement. Methods: All the components of the simulator were manufactured using silicone molding techniques in conjunction with three-dimensional (3D) printing. 3D images sourced from computed tomography scans and colonoscopy images were converted into a stereolithography format. Acrylonitrile butadiene styrene copolymers have been used in fused deposition modeling to produce moldings. Results: The simulator replicated the large intestine from the rectum to the cecum, mimicking the texture and shape of the human colon. It enables training for colonoscopy insertion, cecum intubation, loop reduction, and stenting within stenotic areas. Interchangeable stenotic modules for four sites (rectum, sigmoid colon, descending colon, and ascending colon) were easily assembled for training. These modules integrate tumor contours and blood vessel structures with a translucent center, allowing real-time visualization during stenting. Successful and repeatable demonstrations of stent insertion and expansion using the reusable SEMS were consistently achieved. Conclusions: This innovative simulator offers a secure colonic stenting practice across various locations, potentially enhancing clinical outcomes by improving operator proficiency during actual procedures.

Sensorimotor foundations of speech perception in infancy.

The perceptual system for speech is highly organized from early infancy. This organization bootstraps young human learners' ability to acquire their native speech and language from speech input. Here, we review behavioral and neuroimaging evidence that perceptual systems beyond the auditory modality are also specialized for speech in infancy, and that motor and sensorimotor systems can influence speech perception even in infants too young to produce speech-like vocalizations. These investigations complement existing literature on infant vocal development and on the interplay between speech perception and production systems in adults. We conclude that a multimodal speech and language network is present before speech-like vocalizations emerge.

Optimizing steady-state responses to index statistical learning: Response to Benjamin and colleagues.

Neural entrainment refers to the tendency of neural activity to align with an ongoing rhythmic stimulus. Measures of neural entrainment have been increasingly leveraged as a tool to understand how the brain tracks different types of regularities in sensory input. However, the methods used to quantify neural entrainment are varied, with numerous analytic decision points whose consequences have not been well-characterized. In a valuable contribution to this field, Benjamin, Dehaene-Lambertz and Flo (submitted) systematically compare various methodological approaches for studying neural entrainment. They demonstrate that the use of overlapping epochs, in which sliding time windows are extracted and analyzed, results in an artifactual inflation of entrainment estimates at the frequency of overlap. Here, in response to this updated best practice recommendation, we reanalyzed three previously published datasets that had been previously analyzed with overlapping epochs. Although our main results and conclusions are unaltered from those originally reported, we agree with Benjamin and colleagues that overlapping epochs should generally be avoided in classic analyses of steady-state experiments, which aim to quantify overall peaks in phase or power across an entire experimental duration. However, we present a case that overlapping epochs may be beneficial in fine-grained analyses of neural entrainment over time. The use of overlapping epochs in such analyses could improve temporal resolution without complicating interpretability of the results in cases where the question of interest relates to relative changes in neural entrainment over time within a given frequency.

Neural indicators of articulator-specific sensorimotor influences on infant speech perception.

While there is increasing acceptance that even young infants detect correspondences between heard and seen speech, the common view is that oral-motor movements related to speech production cannot influence speech perception until infants begin to babble or speak. We investigated the extent of multimodal speech influences on auditory speech perception in prebabbling infants who have limited speech-like oral-motor repertoires. We used event-related potentials (ERPs) to examine how sensorimotor influences to the infant's own articulatory movements impact auditory speech perception in 3-mo-old infants. In experiment 1, there were ERP discriminative responses to phonetic category changes across two phonetic contrasts (bilabial-dental /ba/-/ɗa/; dental-retroflex /ɗa/-/ɖa/) in a mismatch paradigm, indicating that infants auditorily discriminated both contrasts. In experiment 2, inhibiting infants' own tongue-tip movements had a disruptive influence on the early ERP discriminative response to the /ɗa/-/ɖa/ contrast only. The same articulatory inhibition had contrasting effects on the perception of the /ba/-/ɗa/ contrast, which requires different articulators (the lips vs. the tongue) during production, and the /ɗa/-/ɖa/ contrast, whereby both phones require tongue-tip movement as a place of articulation. This articulatory distinction between the two contrasts plausibly accounts for the distinct influence of tongue-tip suppression on the neural responses to phonetic category change perception in definitively prebabbling, 3-mo-old, infants. The results showing a specificity in the relation between oral-motor inhibition and phonetic speech discrimination suggest a surprisingly early mapping between auditory and motor speech representation already in prebabbling infants.

Preverbal Infants Discover Statistical Word Patterns at Similar Rates as Adults: Evidence From Neural Entrainment.

The discovery of words in continuous speech is one of the first challenges faced by infants during language acquisition. This process is partially facilitated by statistical learning, the ability to discover and encode relevant patterns in the environment. Here, we used an electroencephalogram (EEG) index of neural entrainment to track 6-month-olds' (N = 25) segmentation of words from continuous speech. Infants' neural entrainment to embedded words increased logarithmically over the learning period, consistent with a perceptual shift from isolated syllables to wordlike units. Moreover, infants' neural entrainment during learning predicted postlearning behavioral measures of word discrimination (n = 18). Finally, the logarithmic increase in entrainment to words was comparable in infants and adults, suggesting that infants and adults follow similar learning trajectories when tracking probability information among speech sounds. Statistical-learning effects in infants and adults may reflect overlapping neural mechanisms, which emerge early in life and are maintained throughout the life span.

Analysis and Differential Expression of Primo Genes Using RNA-Seq and qRT-PCR Experiments.

Although the existence of the primo vasculature system has been shown in many species, including mice, rats, rabbits and humans, the biological role of this system, including expression of genes and proteins, has not yet been investigated. Especially the transcriptional action by mRNA, which is required for biological action, needs to be studied in primo vasculature biology. Differentially expressed genes in both isolated primo vessels and lymphatic vessels of rabbits were analyzed by RNA sequencing experiments. Primer efficiency and RNA purity of the primo vessels under lipopolysaccharides were confirmed prior to performing real-time qRT-PCR analysis following RNA extraction. We demonstrated that FLT4 was enriched in primo vessels and that several genes, including HSPH1 and EPHB2, were highly expressed in primo vessels compared with lymphatic vessels. Our data show that almost all genes, except HSPA4, were increased or sustained in isolated primo vessels compared with lymphatic vessels (FLT4 2.58 fold, HSPH1 1.83 fold, EPHB2 1.52 fold; whereas HSPA4 decreased 0.50 fold), suggesting primo vessels as a central regulator in diverse physiology. This implies that FLT4, HSPH1, and EPHB2 in high amounts may be involved in the functional activity of primo vessels. Our experimental data show that several genes are highly enriched in primo vessels in the lymphatic vessels of the rabbit.

Sensorimotor influences on speech perception in pre-babbling infants: Replication and extension of Bruderer et al. (2015).

The relationship between speech perception and production is central to understanding language processing, yet remains under debate, particularly in early development. Recent research suggests that in infants aged 6 months, when the native phonological system is still being established, sensorimotor information from the articulators influences speech perception: The placement of a teething toy restricting tongue-tip movements interfered with infants' discrimination of a non-native contrast, /Da/-/da/, that involves tongue-tip movement. This effect was selective: A different teething toy that prevented lip closure but not tongue-tip movement did not disrupt discrimination. We conducted two sets of studies to replicate and extend these findings. Experiments 1 and 2 replicated the study by Bruderer et al. (Proceedings of the National Academy of Sciences of the United States of America, 112 (44), 13531-13536, 2015), but with synthesized auditory stimuli. Infants discriminated the non-native contrast (dental /da/ - retroflex /Da/) (Experiment 1), but showed no evidence of discrimination when the tongue-tip movement was prevented with a teething toy (Experiment 2). Experiments 3 and 4 extended this work to a native phonetic contrast (bilabial /ba/ - dental /da/). Infants discriminated the distinction with no teething toy present (Experiment 3), but when they were given a teething toy that interfered only with lip closure, a movement involved in the production of /ba/, discrimination was disrupted (Experiment 4). Importantly, this was the same teething toy that did not interfere with discrimination of /da/-/Da/ in Bruderer et al. (2015). These findings reveal specificity in the relation between sensorimotor and perceptual processes in pre-babbling infants, and show generalizability to a second phonetic contrast.

Differential Gene Expression by RNA-Seq Analysis of the Primo Vessel in the Rabbit Lymph.

For the connectome of primo vascular system, some long-type primo vessels dyed with Alcian blue injected into inguinal nodes, abdominal node, and axially nodes were visualized, which passed over around the vena cava of the rabbit. The Alcian blue dye revealed primo vessels and colored blue in the rabbit lymph vessels. The length of long-type primo vessels was 18 cm on average, of which diameters were about 20-30 µm, and the lymph vessels had diameters of 100-150 µm. Three different tissues of pure primo vessel, mixed primo + lymph vessel, and only lymph vessel were made to undergo RNA-Seq analysis by next-generation sequencing. We also analyzed differentially expressed genes (DEGs) from the RNA-Seq data, in which 30 genes of the primo vessels, primo + lymph vessels, and lymph vessels were selected for primo marker candidates. From the plot of DEG analysis, 10 genes had remarkably different expression pattern on the Group 1 (primo vessel) vs Group 3 (lymph vessel). With Fragments Per Kilobase of exon per Million the cutoff p-value for each gene was < 0.05. Fragments Per Kilobase of exon per Million of the 10 genes such as IGHM, HLA-DRA, HIST1H41, LPL, CD36, SRGN, DGAT2, SNCG, CD48, and GPD1 for primo vessels compared with those of lymph vessels increased twice or thrice. These results suggest that the selected genes could be used for the specific marker to construct primo connectome of circuit system in the rabbit.

Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation.

It is known that the primo vascular system (PVS) includes the primo nodes and vessels. However, the relevant genes in the PVS system for both pathologic and physiologic condition are poorly understood. Here, we first examined the gene expression in primo vessels (PVs) floating in lymphatic endothelium by isolation of PVS and lymphatic vessels (LVs) containing PVS. To investigate therapeutic effects, both PVs and LVs containing PVS were isolated after lipopolysaccharide injection and acupuncture electric stimulation at two acupoints Joksamni (ST36) and Hapgok (LI04) following lipopolysaccharide injection. We used reverse transcriptase-polymerase chain reaction to examine expression of lymphatic endothelial cell markers and inflammatory related genes. We found that lymphatic endothelial cell markers such as fms-related tyrosine kinase 4 (Flt4), lymphatic vessel endothelial receptor (Lyve-1), prospero homeobox protein 1 (Prox-1), and podoplanin (Pdpn) were highly expressed in PV compared to that of lymphatic endothelium, suggesting pivotal roles of PV in LV under inflammation. Furthermore, lymphatic-related genes including metal-response element-binding transcription factor 2 (Mtf2), hypoxia inducible factor (Hif1a), angiotensin II type 1 receptor (Agtr1), and angiotensin II type 2 receptor (Agtr2) were also overall increased in PV, and remarkably increased and these genes except peroxisome proliferator-activated receptor gamma (Pparg) after acupuncture electric stimulation in two acupoints implying central role of PV by gene activation.

tACS-mediated modulation of the auditory steady-state response as seen with MEG.

BACKGROUND: Previous studies have shown that transcranial electrical stimulation can be successfully applied during simultaneous MEG measurements. In particular, using beamforming they have established that changes of stimulus induced as well as evoked activity can be inspected during transcranial alternating current stimulation (tACS). OBJECTIVE/HYPOTHESIS: We studied tACS-mediated changes of the auditory steady-state response (ASSR), hypothesizing that-due to the putatively inhibitory role of alpha oscillations-these evoked responses would be diminished. METHODS: We compared ASSRs in conditions with and without 12-Hz and 6.5-Hz sinusoidal 1.5 mA tACS, applied bilaterally over temporal areas. Source-level activity was estimated using a linearly constrained minimum variance beamformer and compared across tACS conditions using paired t-tests following a condition-internal normalization procedure. CONCLUSIONS: By separating the electrical and auditory stimulation to non-overlapping parts of the frequency spectrum, we were able to compare auditory-evoked steady-state activity across tACS conditions. We observed a significant decrease in normalized ASSR power in the 12-Hz tACS condition, illustrating that tACS could induce immediate changes in auditory evoked activity. This study sets a methodology to further interrogate the causal roles of oscillatory dynamics in auditory cortices, as well as suggests perspectives for employing tACS in clinical contexts.

Does early motor development contribute to speech perception?

At the end of the target article, Keven & Akins (K&A) put forward a challenge to the developmental psychology community to consider the development of complex psychological processes - in particular, intermodal infant perception - across different levels of analysis. We take up that challenge and consider the possibility that early emerging stereotypies might help explain the foundations of the link between speech perception and speech production.

Generation of finite power Airy beams via initial field modulation.

We investigate the finite power Airy beams generated by finite extent input beams such as a Gaussian beam, a uniform beam of finite extent, and an inverse Gaussian beam. Each has different propagation behavior: A finite Airy beam generated by a uniform input beam keeps its Airy profile much longer than the conventional finite Airy beam. Also, an inverse Gaussian beam generates a finite Airy beam with a good bent focusing in free space. In this paper, the analysis and experimental results of finite Airy beams are presented.

Tunable subwavelength hot spot of dipole nanostructure based on VO2 phase transition.

We propose a novel approach to generate and tune a hot spot in a dipole nanostructure of vanadium dioxide (VO2) laid on a gold (Au) substrate. By inducing a phase transition of the VO2, the spatial and spectral distributions of the hot spot generated in the feed gap of the dipole can be tuned. Our numerical simulation based on a finite-element method shows a strong intensity enhancement difference and tunability near the wavelength of 678 nm, where the hot spot shows 172-fold intensity enhancement when VO2 is in the semiconductor phase. The physical mechanisms of forming the hot spots at the two-different phases are discussed. Based on our analysis, the effects of geometric parameters in our dipole structure are investigated with an aim of enhancing the intensity and the tunability. We hope that the proposed nanostructure opens up a practical approach for the tunable near-field nano-photonic devices.

Dual Airy beam.

We derive a general form of Airy wave function which satisfies paraxial equation of diffraction. Based on this, we propose a new form of Airy beam, which is composed of two symmetrical Airy beams which accelerate mutually in the opposite directions. This 'dual' Airy beam shows several distinguishing features: it has a symmetric transverse intensity pattern and improved self-regeneration property. In addition, we can easily control the propagation direction. We also propose 'quad' Airy beam, which forms a rectangular shaped optical array of narrow beams that travel along a straight line. We can control its propagation direction without changing transverse intensity patterns. These kinds of superposed optical beams are expected to be useful for various applications with their unique properties.

Optical beam focusing with a metal slit array arranged along a semicircular surface and its optimization with a genetic algorithm.

A metal slit array arranged along a semicircular surface achieving subwavelength optical beam focusing along the lateral direction is proposed. Taking into consideration surface plasmon polaritons that pass through a metal slit array, we design the array with a curvature. By use of a genetic algorithm, the size of the metal slit and the corresponding curvature are to be determined. Based on our metal slit array configuration, the full width at half-maximum can be achieved on a subwavelength scale.