Activation of salt-inducible kinase 2 promotes the viability of peritoneal mesothelial cells exposed to stress of peritoneal dialysis.

Maintaining mesothelial cell viability is critical to long-term successful peritoneal dialysis (PD) treatment. To clarify the viability mechanism of peritoneal mesothelial cells under PD solutions exposure, we examined the mechanisms of cellular response to this stress conditions. Here we report that the proteasome activity is inhibited when treated with PD solutions. Proteasome inhibition-mediated activation of salt-inducible kinase 2 (SIK2), an endoplasmic reticulum-resident protein, is important for mesothelial cell viability. SIK2 is mobilized to promote autophagy and protect the cells from apoptosis under PD solution or MG132 treatment. Immunofluorescence staining showed that SIK2 is colocalized with LC3B in the autophagosomes of mesothelial cells treated with PD solution or derived from patients undergoing PD treatment. SIK2 activation is likely via a two-step mechanism, upstream kinases relieving the autoinhibitory conformation of SIK2 molecule followed by autophosphorylation of Thr175 and activation of kinase activity. These results suggest that activation of SIK2 is required for the cell viability when proteasome activity is inhibited by PD solutions. Maintaining or boosting the activity of SIK2 may promote peritoneal mesothelial cell viability and evolve as a potential therapeutic target for maintaining or restoring peritoneal membrane integrity in PD therapy.

Residual stresses of sputtering titanium thin films at various substrate temperatures.

This work seeks to characterize the residual stresses of titanium thin films as they are affected by various substrate temperatures during the sputtering process. The titanium thin films are deposited on silicon wafers by a RF magnetron sputter while different substrate temperatures are considered. The residual stresses are measured by both X-ray diffraction and a substrate curvature method, and consistent results are obtained by both methods. The results show that the residual stress decreases as the substrate temperature increases, in which the stress changes from tensile to compressive when the substrate temperature increases from 25 to 50 degrees C. Furthermore, the elastic modulus and hardness of the titanium thin films are tested with a nanoindenter using a standard Berkovich probe. Correlations between the residual stresses and mechanical properties measured by nanoindentation are also discussed.

An efficient fault-tolerant out-patient order entry system based on special distributed client/server architecture.

An automatic order entry system is very important for processing out-patient information. This system not only helps physicians to enter their orders directly, but can also reduce order communication error and thus improve medical quality. Therefore, many hospitals have high aspirations to generate and implement direct order entry systems, but they are also concerned about the setbacks of system failure. In this paper, we present an effective and efficient fault-tolerant order entry system based on special distribution client/server architecture that satisfies the requirements of out-patient order entry very well. From the experimental results carried out on a prototype, we found that this system can improve the system response time of order entry and can also generate an operational method having a user friendly interface. The physicians can enter their orders easily, accurately, directly, flexibly and at a faster rate by making choices from standardized and personalized menus in this system.

An efficient fault-tolerant order entry management information system based on special distributed client/server architecture.

An automatic order entry system is very important for the processing of out-patient information, not only helping doctors to enter their orders directly but also reducing errors of communication. Many hospitals are anxious to set up a direct order entry system but are concerned about possible system failures. In this paper we report on an effective and efficient fault-tolerant order entry management system which satisfies the requirements for out-patient order entry. From the results of experiments on a prototype we found that the system was user friendly and reduced the time taken. Doctors are able to enter their orders more easily, accurately and quickly by selecting from the standardized and personalized menus to be found in the system.