Phytophotodermatitis related to carrot extract-containing sunscreen.

Phytophotodermatitis is a clinical diagnosis from phototoxicity of the skin induced by contact with plants or their extracts. Phytophotodermatitis maypresent with burning, erythema, patches, plaques, vesicles, bullae, or hyperpigmented patches in welldemarcated and unusual shapes. Inquiring about occupation, hobbies, and plant or plant extract contact is essential to establishing the diagnosis. Herein we present a case of phytophotodermatitisafter use of carrot extract-containing sunscreen presenting as a hyperpigmented patch in a geometric distribution with accentuation of pigment within the dynamic rhytides.

Pain-like behaviour and spinal changes in the monosodium iodoacetate model of osteoarthritis in C57Bl/6 mice.

BACKGROUND: Osteoarthritis (OA) is a highly prevalent, age-related pain condition that poses a significant clinical problem. Here, in the monosodium iodoacetate (MIA) model of OA, we have characterized pain behaviours and associated changes at the first pain synapse in the dorsal horn of the spinal cord. METHODS: Mice received intra-articular injections of 0.5, 0.75 and 1 mg MIA and mechanical paw withdrawal threshold was monitored for up to 4 weeks. An intrathecal injection of peptide antagonist calcitonin gene-related peptide (CGRP8-37 ) was given 3 weeks post MIA and paw withdrawal thresholds were measured after 1 and 3 h. Immunohistochemical analysis of the lumbar dorsal horn was carried out and activity-evoked CGRP release was measured from isolated lumbar dorsal horn slices - with dorsal roots attached. RESULTS: By 2 weeks after intra-articular MIA injection, mechanical hypersensitivity was established in the ipsilateral hindpaw. There was no evidence of sensory neuron damage in lumbar dorsal root ganglia 7 days after 1 mg MIA. However, both dorsal horn neuron activation and microglial response (Fos and Iba-1 immunostaining) but not reactive astrocytes (glial fibrillary acidic protein) were observed. Evoked CGRP release was greater from dorsal horn slices of MIA-treated mice compared with control. Furthermore, intrathecal administration of peptide antagonist CGRP8-37 acutely attenuated established MIA-induced mechanical hypersensitivity. CONCLUSIONS: Intra-articular MIA is associated with referred mechanical hypersensitivity and increased release of CGRP from primary afferent fibres in the dorsal horn where second-order neuron activation is associated with a microglial response. Antagonism of CGRP receptor activation provides a therapeutic avenue for the treatment of pain in OA.

Determination of Co(II) by chemiluminescence after in situ electrochemical pre-separation on a flow-through mercury film electrode.

A novel method of electrochemical pre-separation of Co(II) before detection by chemiluminescence is reported together with the associated instrumentation. The Co(II) ions were selectively pre-separated on a mercury film electrode (MFE) by on-line reduction, then the accumulated metal was oxidised and selectively stripped back into the flowing solution as Co(II). These secondary ions were quantified as a result of their catalytic activity on the chemiluminescent reaction between luminol and hydrogen peroxide that was also induced on-line. The whole sequence was carried out in an automated flow-through system, in which the electrochemical pre-separation of metals was performed in either continuous flow or flow injection analysis (FIA) regimes. The scope of the method, both in terms of selectivity and sensitivity, has been demonstrated and the quantitative determination of Co(II) by the proposed method has been investigated. For a period of continuous flow pre-separation of 4 min, the calibration curve for Co(II) was linear up to a concentration of 100 micrograms l-1, the relative standard deviation was 4% at the 20 micrograms l-1 level and the limit of detection was 0.5 microgram l-1 (at the 3 sigma level). The method was applied to the determination of the cobalt content in a high purity iron sample.

Staff perceptions of sadness among people with mental handicaps.

This study investigated the use of an informant behaviour checklist to examine carestaff perceptions of sadness among a hospital population of people with mental handicaps. Using Cohen's kappa statistic there were only three checklist items which had acceptable levels of inter-rater agreement (physical aggression, crying and verbal abuse) with no significant variation in the incidence of those behaviours according to the severity of mental handicap. The findings suggest that future research might attend to concepts of sadness among carestaff and their ability to identify sad emotional states in a reliable manner.

Responses to controlling gastrointestinal parasitism in dairy cattle.

Sixty-eight Holstein heifers, paired on milk production potential and season of birth, were randomly assigned within pairs to a control or treatment group to evaluate the effect of raising replacement heifers on a prophylactic anthelmintic regimen. The anthelmintic, thiabendazole (6.6 g/100 kg body weight), was administered orally as a paste at 2.5, 3.5, 6, 9, and 14 mo of age and at parturition. The control group received no thiabendazole. Fecal samples were collected from both groups at the time of thiabendazole treatment. Days to first service were 457.1 and 457.8 for the control and treatment groups. There were no differences in services per conception or days to first calving. Nematode eggs per 5 g of feces were similar at 2.5 mo (.6 and .8), 3.5 mo (38.6 and 22.4), 6 mo (40.2 and 43.1), 9 mo (39.0 and 22.4), 14 mo (55.2 and 22.4), or parturition (16.5 and .4). There were no differences in body weight changes over the first 18 mo of age. Subsequent first lactations were similar (9422 and 8710 kg 305-d mature equivalent, for twice daily milking) as was actual milk production for the first 120 d of lactation. Under these environmental conditions and apparently low parasitism in this herd, thiabendazole did not substantially influence nematode egg count and had no effects on body weight gains or milk production.

Whole rolled sunflower seeds with or without additional limestone in lactating dairy cattle rations.

Thirty lactating Holstein cows were in a continuous trial from 21 to 120 days postpartum to evaluate diets containing whole, rolled sunflower seeds with or without additional limestone. Cows were fed individually total mixed rations of (dry matter) 47% corn silage, 9% alfalfa hay, and 44% concentrate. Concentrates were corn and soybean meal (control); corn, soybean meal, and 22% sunflower seeds; or corn, soybean meal, and sunflower seeds plus 3.5% additional limestone. Milk yield (32.2, 32.0, and 32.8 kg/day) was similar among rations. Yield of 4% fat-corrected milk was lower for cows fed sunflower seeds without additional limestone (30.2, 28.1, and 30.2 kg/day) because of lower milk fat percentages (3.57, 3.19, and 3.51). Milk protein percentage tended to be lower for cows fed sunflower seeds with additional limestone (3.01, 2.97, and 2.90). Milk, flavor score was acceptable but tended to be lower for milk from cows fed sunflower seeds with additional limestone (8.4, 8.5, and 7.9). Milk fat from cows fed sunflower seed rations contained less carbon-14:0, 16:0, and 16:1 fatty acids but more carbon-18:0. Dry matter intakes were 21.0, 18.4, and 20.0 kg/day. Dry matter digestibilities, body weight changes, and ruminal volatile fatty acid concentrations were similar among treatments. Total cholesterol in blood serum was elevated in cows fed sunflower seed rations. Insoluble salts of fatty acids were increased in ruminal fluid dry matter from cows fed sunflower seeds but were not increased further by additional limestone. Concentrations of nonesterified carbon-18:1 fatty acids in ruminal fluid dry matter were lower for cows fed sunflower seeds with additional limestone.

Raising dairy replacement heifers: a review of NC-119 cooperative research.

Scientists at 16 experiment stations have been involved in cooperative research to study methods of increasing efficiency and for improving management of dairy replacements. This research is part of the Regional Research Project NC-119 "Improving Large Dairy Herd Management Practices." Research objectives have concerned means of increasing efficiency of producing replacements for large dairy herds. Through cooperative research, important answers were found to questions involving nutrition, housing, and management for replacement animals. Between 1977 and 1982, over 50 articles have been published in journals and trade magazines on replacement animal rearing from research of the NC-119 project. These results should have wide impact and use on livestock management.

Effect of methionine hydroxy analog supplementation on dairy cattle hoof growth and composition.

Fifty lactating Holstein cows were assigned randomly to one of two treatments, control and control plus approximately 30 g methionine hydroxy analog, and confined on concrete for 11 mo. The control diet consisted of sorghum silage and concentrate fed as a blended ration. Sulfur contents of dry matter were .12% and .16% for control and methionine hydroxy analog rations. Hoof growth and hardness were measured on front and rear right abaxial claws in the dorsal and lateral regions. Hoof growth rates were measured for four periods; summer-fall, fall-winter, winter-spring, and spring-summer, each 70 to 90 days. Hooves of cows fed methionine hydroxy analog grew faster than those of control cows during spring-summer in all regions. Variations of growth rates of hooves were seasonal and tended to follow variations in daily photoperiod. Wear rates were not affected significantly by treatment. Hooves of cows fed methionine hydroxy analog were softer in the top dorsal region at the end of winter-spring and in the dorsal toe region at the end of spring-summer. All other locations were not affected significantly by treatment. The toe region was harder than the top of the hoof. Cows fed methionine hydroxy analog had less cysteine and proline in hoof than control cows and greater percentages of methionine lysine, tyrosine, and glutamic acid. These results suggest that a decrease of disulfide bonding occurred in the hoof tissue of cows fed methionine hydroxy analog. Cows fed methionine hydroxy analog produced more actual milk, milk fat, and 4% fat-corrected milk during 180 days than did control cows.