RESUMEN
One new chromone 3,3-dimethylallylspatheliachromene methyl ether (1), as well as five known chromones, 6-(3-methylbut-2-enyl) allopteroxylin methyl ether (2), 6-(3-methylbut-2-enyl) allopteroxylin (3), 3,3-dimethylallylspatheliachromene (4), 5-O-methylcneorumchromone K (5) and spatheliabischromene (6), two alkaloids, 8-methoxy-N-methylflindersine (7) and 8-methoxyflindersine (8), and two limonoids, limonin diosphenol (9) and rutaevin (10), were isolated from Dictyoloma vandellianum A. Juss (Rutaceae). Cytotoxic activities towards tumor cell lines B16-F10, HepG2, K562 and HL60 and non-tumor cells PBMC were evaluated for compounds 1 - 6. Compound 1 was the most active showing IC50 values ranging from 6.26 to 14.82 µg/ml in B16-F10 and K562 cell lines, respectively, and presented IC50 value of 11.65 µg/ml in PBMC cell line.
Asunto(s)
Cromonas/química , Rutaceae/química , Alcaloides/química , Alcaloides/aislamiento & purificación , Alcaloides/toxicidad , Animales , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Cromonas/aislamiento & purificación , Cromonas/toxicidad , Células HL-60 , Células Hep G2 , Humanos , Células K562 , Leucocitos Mononucleares/citología , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/metabolismo , Limoninas/química , Limoninas/aislamiento & purificación , Limoninas/toxicidad , Espectroscopía de Resonancia Magnética , Ratones , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Rutaceae/metabolismoRESUMEN
Phytochemical investigation of the bark of Guatteria friesiana afforded 12 new aporphines (1-12), along with nine known alkaloids (13-21). The structures of the new alkaloids were determined on the basis of spectroscopic data interpretation. The cytotoxic activity of the isolated compounds against a small panel of tumor cell lines was assessed using the Alamar blue assay.