Immunome differences between porcine ileal and jejunal Peyer's patches revealed by global transcriptome sequencing of gut-associated lymphoid tissues.

The epithelium of the intestinal mucosa and the gut-associated lymphoid tissues (GALT) constitute an essential physical and immunological barrier against pathogens. In order to study the specificities of the GALT transcriptome in pigs, we compared the transcriptome profiles of jejunal and ileal Peyer's patches (PPs), mesenteric lymph nodes (MLNs) and peripheral blood (PB) of four male piglets by RNA-Seq. We identified 1,103 differentially expressed (DE) genes between ileal PPs (IPPs) and jejunal PPs (JPPs), and six times more DE genes between PPs and MLNs. The master regulator genes FOXP3, GATA3, STAT4, TBX21 and RORC were less expressed in IPPs compared to JPPs, whereas the transcription factor BCL6 was found more expressed in IPPs. In comparison between IPPs and JPPs, our analyses revealed predominant differential expression related to the differentiation of T cells into Th1, Th2, Th17 and iTreg in JPPs. Our results were consistent with previous reports regarding a higher T/B cells ratio in JPPs compared to IPPs. We found antisense transcription for respectively 24%, 22% and 14% of the transcripts detected in MLNs, PPs and PB, and significant positive correlations between PB and GALT transcriptomes. Allele-specific expression analyses revealed both shared and tissue-specific cis-genetic control of gene expression.

Quantitative effects of an intronic retroviral insertion on the transcription of the tyrosinase gene in recessive white chickens.

Recently, we reported the complete association of a retroviral insertion in intron 4 of the tyrosinase gene and the recessive white mutation (c) in chickens. The mutant allele carrying the retroviral insertion produced, in skin samples of 10-week-old chickens, aberrant tyrosinase transcripts that did not contain exon 5. In the present study, we performed serial molecular and statistical analyses on embryos and 10-week-old chickens to characterize the quantitative effect of the retroviral insertion on the expression pattern of tyrosinase in different tissues (skin and retina). By using quantitative real-time RT-PCR, we observed that the expression level of tyrosinase was significantly lower in recessive white chickens than in wild-type coloured chickens, but that this pattern was age- and tissue-dependent. The differential expression in skin was not significant in embryos, whereas it was highly significant in 10-week-old chickens. Furthermore, there was no difference in the expression of tyrosinase in the retinal pigment epithelium of animals with different genotypes; this corresponds to phenotypic data, which show pigmented eyes in both genotypes. These findings show that the retroviral insertion disturbs tyrosinase expression in the recessive white mutant chickens, and suggests that the regulation of tyrosinase expression in chickens differs between embryos and growing animals, as well as between skin and retina.

Genetic similarity and relationships of DNA fingerprints with performance and with heterosis in Japanese quail lines from two origins and under reciprocal recurrent or within-line selection for early egg production.

DNA fingerprints of Japanese quail male and female pure line breeders were obtained with probes 33.6, 33.15, and R18.1 and they yielded a total of 59 scoreable bands. Bandsharing (0

Molecular characterization of endogenous viral genes of the avian leukosis virus family in an experimental population of brown-egg layers.

Retroviral DNA sequences similar to the exogenous avian leukosis virus can be found in the genome of many chicken breeds and have been identified as the ALVE family of endogenous viral (ev) genes. Most of them have been described by a restriction fragment length polymorphism (RFLP) procedure with two restriction enzymes and a full length viral probe. In order to facilitate the comparison of ALVE genes between strains, the nomenclature workshop held at the XXIV International Society for Animal Genetics Congress recommended that four enzymes and several viral subprobes be used to characterize each locus. This approach has been followed in the present study of a Rhode Island Red experimental population. A previous study had identified ev genes with the SacI and BamHI enzymes and the Rous-associated virus-2 probe (RAV-2). Chickens carrying only one ALVE locus at a time have been produced to facilitate the analysis. Additional enzymes (EcoRI, HindIII, and KpnI), the full probe RAV-2 and three viral subprobes for the gag, pol, and LTR regions have been used. In addition, a PCR diagnostic test has been used to search for homologies with the ALVE1 (= ev1), ALVE6 (= ev6) and evA loci. Currently, 12 loci have been identified precisely: three were identical to ALVE loci described previously, either in White Leghorns, ALVE6 and ALVE18 (= ev18) or in broilers (evB8). In addition, the evB8 locus was found to be identical to the evA locus previously described in brown-egg layers. Nine loci appeared specific to this Rhode Island Red population. Four of these specific loci were complete and one of them could be considered of characteristic of this population, because of its very high frequency. The remaining five specific loci showed small deletions, either in the pol region for one of them or in the env region for three of them or at the 3' long terminal repeat for one of them. Altogether, 5 out of 12 loci were structurally complete, which could suggest that deleted proviruses may have been preferentially retained.

The wheat mitochondrial rps13 gene: RNA editing and co-transcription with the atp6 gene.

Northern analyses and reverse transcription-polymerase chain-reaction (RT-PCR) experiments, followed by PCR amplification product sequencing, were performed on total mitochondrial (mt) RNAs from wheat seedlings and tissue cultures. It was shown that the rps13 gene, which encodes ribosomal protein S13, and the atp6 gene, which encodes subunit 6 of the ATP synthase complex, were co-transcribed. However, rps13 transcripts were virtually undetectable in seedlings under conditions where atp6 transcripts appeared abundant. In addition, markedly higher steady state transcript levels were observed in tissue culture. Expression of the mitochondrial rps13 gene was confirmed by showing that its transcripts were edited. Slight differences between editing patterns of tissue-culture and whole-plant transcripts were found. Taken together, these results suggest that in vitro culture could disturb the post-transcriptional regulation of gene expression.