RESUMEN
Dongan black chicken (Gallus gallus domesticus, DBC) is one of the famous native breed of Hunan province in China. The complete mitochondrial genome sequence plays an important role in the accurate determination of phylogenetic relationships among metazoans. It is the first time that the complete mitochondrial genome sequence of the DBC was reported. The total length of the mtDNA is 16,785 bp, It contains the typical structure, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 1 non-coding control region (D-loop region). The overall composition of the mtDNA was estimated to be 30.27% for A, 23.78% for T, 32.46% for C, and 13.49% for G. Phylogenetic analyses using N-J computational algorithms showed that the analyzed 21 Galliformes species are divided into four major clades: Phasianidae, Numidiidae, Odontophoridae, and Megapodiidae. In addition, our work confirmed that DBC and Taoyuan chicken (TYC) have a close genetic relationship with fellow tribal members Xuefeng black-boned chicken (XBC) and Huang Lang chicken (HLC). Meanwhile, we also found that DBC and TYC have highly similar genetic relationship. This work will provide an important data set for the study of genetic mechanism of chicken in Hunan province.
RESUMEN
In this study, we cloned and sequenced the complete mitochondrial DNAs of Chinese goose, Anser cygnoides populations from three different areas of Hunan province in China. The Anser cygnoides breed Wugangtong white goose (WGTW) sample and Wugangtong grey goose sample (WGTG) were taken from the Wugang county of Shaoyang city, the Anser cygnoides breed Xupu goose (XP) sample was taken from the Xupu county of Huaihua city, and the Anser cygnoides breed Yanling white goose (YLW) sample was taken from the Yanling county of Zhuzhou city. The organization of the four Anser cygnoides breeds mitochondrial genomes was similar. Phylogenetic analyses using N-J computational algorithms showed that the analyzed species are divided into four major clades: Anatinae, Anserinae, Dendrocygninae and Anseranatidae. It was noted that Wugangtong white goose, Yanling white goose and Xupu goose have highly similar phylogenetic relationship.
Asunto(s)
ADN Mitocondrial , Gansos/clasificación , Gansos/genética , Genoma Mitocondrial , Filogenia , Animales , Composición de Base , China , Genes Mitocondriales , Tamaño del Genoma , Sistemas de Lectura Abierta , Análisis de Secuencia de ADN , Secuenciación Completa del GenomaRESUMEN
Wugangtong white goose is one of the famous native breed in Hunan province of China. In this study, the complete mitochondrial genome sequence of the Wugangtong white goose was reported in Hunan Province first, which was determined through PCR-based method. The total length of the mitogenome is 16,741 bp. It contains the typical structure, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes and 1 non-coding control region (D-loop region) as that of most other vertebrates. The overall composition of the mitogenome was estimated to be 30.22% for A, 22.69% for T, 32.02% for C and 15.06% for G. All the protein initiation codons are ATG, except for COX1, COX2 and ND5 are GTG, ND6 is CTA. The complete mitochondrial genome sequence of the Wugangtong white goose will provide an important data set for the study in genetic mechanism of Wugangtong goose in Hunan province.
Asunto(s)
Gansos/genética , Genoma Mitocondrial/genética , Animales , China , Codón Iniciador/genética , ADN Mitocondrial/química , ADN Mitocondrial/genética , Mitocondrias/genética , Sistemas de Lectura Abierta , ARN Ribosómico/genética , ARN de Transferencia/genética , Análisis de Secuencia de ADNRESUMEN
In this study, the complete mitochondrial genome sequence of the Yanling white goose was reported in Human Province first, which was determined through PCR-based method. The total length of the mitogenome is 16,742 bp. It contains the typical structure, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes and 1 non-coding control region (D-loop region) as that of most other vertebrates. The overall composition of the mitogenome was estimated to be 30.22% for A, 22.70% for T, 32.02% for C and 15.07% for G. All the protein initiation codons are ATG, except for COX1, COX2 and ND5 are GTG, ND6 is CTA. The complete mitochondrial genome sequence of the Yanling white goose in Human Province provides an important data set for the study in genetic mechanism of poultry.
Asunto(s)
Gansos/genética , Genoma Mitocondrial/genética , Animales , Organismos Acuáticos/genética , Composición de Base/genética , Codón Iniciador/genética , ADN Mitocondrial/genética , Genes Mitocondriales/genética , Tamaño del Genoma/genética , Sistemas de Lectura Abierta/genética , ARN Ribosómico/genética , ARN de Transferencia/genética , Análisis de Secuencia de ADN/métodosRESUMEN
Xupu goose is one of the famous native breed in China. In this work we reported the complete mitochondrial genome sequence of the Xupu goose in Hunan Province for the first time. The total length of the mitogenome is 16,742 bp, with the base composition of 30.21% for A, 22.70% for T, 32.02% for C, 15.08% for G, in the order C > A > T > G feature occurring in the Xupu goose. And it is made up of two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and a non-coding control region (D-loop region). The characteristics of the mitochondrial genome were analyzed and discussed in detail. The complete mitochondrial genome sequence of Xupu goose will be useful for the phylogenetics of poultry, and be available as basic data for the genetics and breeding.
Asunto(s)
Gansos/genética , Genoma Mitocondrial/genética , Animales , Organismos Acuáticos/genética , China , Genes Mitocondriales/genética , Sistemas de Lectura Abierta/genética , ARN Ribosómico/genética , ARN de Transferencia/genética , Análisis de Secuencia de ADN/métodosRESUMEN
In this study, the complete mitochondrial genome sequence of the Linwu duck was first reported in Human Province, which was determined through PCR-based method. Linwu duck is one of the famous native breed in China. The total length of the mitogenome is 16,604 bp, with the base composition of 29.20% for A, 22.21% for T, 32.82% for C, 15.78% for G, in the order C > A > T > G feature occurring in the Linwu duck. It is made up of 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and one non-coding control region (D-loop region). The complete mitochondrial genome sequence of the Linwu duck in Human Province provides an important data for further study about genetic mechanism and breeding.
Asunto(s)
Patos/genética , Genoma Mitocondrial/genética , Animales , Composición de Base/genética , Cruzamiento/métodos , China , Sistemas de Lectura Abierta/genética , ARN Ribosómico/genética , ARN de Transferencia/genéticaRESUMEN
Youxian duck is one of the famous native breed in China. In this work we reported the complete mitochondrial genome sequence of the Youxian duck in Human Province for the first time. The total length of the mitogenome is 16,606 bp, with the base composition of 29.21% for A, 22.18% for T, 32.83% for C, 15.78% for G, in the order C > A > T > G feature occurring in the Youxian duck. It is made up of two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and one non-coding control region (D-loop region). The complete mitochondrial genome sequence of Youxian duck will be useful for the phylogenetics of poultry, and be available as basic data for the genetics and breeding.
Asunto(s)
Patos/genética , Genoma Mitocondrial/genética , Animales , Composición de Base/genética , Cruzamiento/métodos , China , Sistemas de Lectura Abierta/genética , ARN Ribosómico/genética , ARN de Transferencia/genética , Análisis de Secuencia de ADN/métodosRESUMEN
Mitochondrial DNA plays an important role in living organisms. In this study, the complete mitochondrial genome sequence of the Wugangtong grey goose was reported in Human Province first, which was determined through PCR-based method. The total length of the mitogenome is 16,740 bp. It contains the typical structure, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes and 1 non-coding control region (D-loop region) as that of most other vertebrates. The overall composition of the mitogenome was estimated to be 30.22% for A, 22.67% for T, 32.05% for C and 15.07% for G. All the protein initiation codons are ATG, except for COX1, COX2 and ND5 are GTG, ND6 is CTA. The complete mitochondrial genome sequence of the Wugangtong grey goose will provides an important data set for the study in genetic mechanism of goose in Hunan province.