ABSCISIC ACID INSENSITIVE 3 promotes auxin signalling by regulating SHY2 expression to control primary root growth in response to dehydration stress.

Plants combat dehydration stress through different strategies including root architectural changes. Here we show that when exposed to varying levels of dehydration stress, primary root growth in Arabidopsis is modulated by regulating root meristem activity. Abscisic acid (ABA) in concert with auxin signalling adjust primary root growth according to stress levels. ABSCISIC ACID INSENSITIVE 3 (ABI3), an ABA-responsive transcription factor, stands at the intersection of ABA and auxin signalling and fine-tunes primary root growth in response to dehydration stress. Under low ABA or dehydration stress, induction of ABI3 expression promotes auxin signalling by decreasing expression of SHY2, a negative regulator of auxin response. This further enhances the expression of auxin transporter gene PIN1 and cell cycle gene CYCB1;1, resulting in an increase in primary root meristem size and root length. Higher levels of dehydration stress or ABA repress ABI3 expression and promote ABSCISIC ACID INSENSITIVE 5 (ABI5) expression. This elevates SHY2 expression, thereby impairing primary root meristem activity and retarding root growth. Notably, ABI5 can promote SHY2 expression only in the absence of ABI3. Such ABA concentration-dependent expression of ABI3 therefore functions as a regulatory sensor of dehydration stress levels and orchestrates primary root growth by coordinating its downstream regulation.

Competitive Charge Separation Pathways in a Flexible Molecular Folda-Dimer.

We report the photophysical properties of a molecular folda-dimer system PDI-AnEt2-PDI, where the electron-donating N,N-diethylaniline (AnEt2) moiety bridges two electron-accepting perylene diimide (PDI) chromophores. The conformationally flexible PDI-AnEt2-PDI adopts either an open (two PDIs far apart) or folded (two PDIs within π-stacking distance) conformation, depending on the solvent environment. We characterized the photoinduced charge separation dynamics of both open and folded forms in solvents of varying polarity. The open form undergoes charge separation to give PDI•--AnEt2•+-PDI (Bridge electron transfer) independent of solvent polarity. The folded form exhibits two charge separation photoproducts, yielding both PDI•--AnEt2•+-PDI and PDI•--AnEt2-PDI•+, the latter of which is formed via symmetry-breaking charge separation (SBCS) between the two π-stacked PDI chromophores. Our results further indicate that the conformational flexibility of the folda-dimer leads to unexpected excimer formation in some open form conditions. In contrast, no excimer formation is observed in the folded form, indicating that this geometry preferentially yields the SBCS instead. Our results provide insight into how conformationally flexible folda-dimer systems can be designed and built to tune competitive photophysical pathways.

Recent Advances in Organic Molecular-to-Supramolecular Self-Assembled Room-Temperature Phosphorescent Materials for Biomedical Applications.

This minireview focuses on recent advancements in organic molecular-to-supramolecular self-assembled room-temperature phosphorescent (RTP) materials and their prospective biomedical applications. RTP materials, having their unique capacity to emit long-lasting phosphorescence at ambient temperature, have piqued researchers' interest in various biological applications, including biosensing, bioimaging, drug delivery, and photodynamic therapy (PDT). These materials have several benefits, including high sensitivity, remarkable photostability, and low cytotoxicity. RTP materials' self-assembly into supramolecular structures improves their performance and broadens their uses. Researchers have built organic RTP systems with long-lasting phosphorescence by leveraging weak noncovalent interactions in aquatic conditions. These materials have demonstrated incredible promise as biosensors that enable sensitive analyte detection and as photosensitizers in PDT that target and sensitize specific cell types. The review also outlines future directions and challenges in developing and utilizing pure organic RTP materials for biological imaging purposes, providing valuable guidelines for their future design and application.

RAV1 mediates cytokinin signaling for regulating primary root growth in Arabidopsis.

Root growth dynamics is an outcome of complex hormonal crosstalk. The primary root meristem size, for example, is determined by antagonizing actions of cytokinin and auxin. Here we show that RAV1, a member of the AP2/ERF family of transcription factors, mediates cytokinin signaling in roots to regulate meristem size. The rav1 mutants have prominently longer primary roots, with a meristem that is significantly enlarged and contains higher cell numbers, compared with wild-type. The mutant phenotype could be restored on exogenous cytokinin application or by inhibiting auxin transport. At the transcript level, primary cytokinin-responsive genes like ARR1, ARR12 were significantly downregulated in the mutant root, indicating impaired cytokinin signaling. In concurrence, cytokinin induced regulation of SHY2, an Aux/IAA gene, and auxin efflux carrier PIN1 was hindered in rav1, leading to altered auxin transport and distribution. This effectively altered root meristem size in the mutant. Notably, CRF1, another member of the AP2/ERF family implicated in cytokinin signaling, is transcriptionally repressed by RAV1 to promote cytokinin response in roots. Further associating RAV1 with cytokinin signaling, our results demonstrate that cytokinin upregulates RAV1 expression through ARR1, during post-embryonic root development. Regulation of RAV1 expression is a part of secondary cytokinin response that eventually represses CRF1 to augment cytokinin signaling. To conclude, RAV1 functions in a branch pathway downstream to ARR1 that regulates CRF1 expression to enhance cytokinin action during primary root development in Arabidopsis.

Reversible Supramolecular Polymorphism in Solution and Solid Matrix by Manipulating Sidegroup Conformation.

Reversible switching between supramolecular polymorphs offers a great way to introduce stimuli-responsiveness. Supramolecular polymorphism is usually achieved through pathway complexity, or by exploiting solvent-solute interactions. But, steering a self-assembly along a specific pathway to form a kinetically-stable aggregate is not easy. Also, changing solvent to switch between polymorphs is impractical. We present a perylene bisimide molecule with a trans-azobenzene sidegroup that assembles into three supramolecular polymorphs with distinct colors, morphologies, packing and aggregation mechanism. Optical absorption and FTIR spectroscopy reveal the importance of hydrogen-bonding interaction between protic solvent and azo N that controls the planarity of the azobenzene group and influences molecular packing. This interaction can be further modulated using temperature, and solution pH to reversibly switch between the three polymorphs, in solution as well as in solid silica-gel matrix.