RESUMEN
Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMérieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level: MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.
Asunto(s)
Técnicas de Tipificación Bacteriana/métodos , Análisis de Secuencia de ADN/métodos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Infecciones Estreptocócicas/diagnóstico , Infecciones Estreptocócicas/microbiología , Streptococcus/clasificación , Streptococcus/aislamiento & purificación , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Intergénico/química , ADN Intergénico/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Humanos , ARN Ribosómico 16S/genética , Streptococcus/química , Streptococcus/genéticaRESUMEN
Point prevalence of Cryptosporidium and Giardia carriage among 230 asymptomatic preschool children attending day-care facilities was 1.3% (95% CI: 0.3%-3.8%) for each parasite, with no dual infections. Cryptosporidium oocysts were detected by immunomagnetic separation and immunofluorescence microscopy and genotyped: one isolate was Cryptosporidium hominis, the other 2 being skunk and cervine genotypes, rarely found in symptomatic human infection.