Transgenic black soldier flies for production of carotenoids.

The black soldier fly (BSF), Hermetia illucens, has gained traction recently as a means to achieve closed-loop production cycles. BSF can subsist off mammalian waste products and their consumption of such waste in turn generates compost that can be used in agricultural operations. Their environmental impact is minimal and BSF larvae are edible, with a nutritional profile high in protein and other essential vitamins. Therefore, it is conceivable to use BSF as a mechanism for both reducing organic waste and maintaining a low-impact food source for animal livestock or humans. The main drawback to BSF as a potential human food source is they are deficient in fat-soluble vitamins such as Vitamins A, D, and E. While loading BSF with essential vitamins may be achieved via diet-based interventions, this undercuts the goal of a closed-loop as specialized diets would require additional supply chains. An alternative is to genetically engineer BSF that can synthesize these essential vitamins. Here we describe a BSF line that has been engineered with the two main carotenoid biosynthetic genes, CarRA and CarB for production of provitamin carotenoids within the Vitamin A family. Our data describe the manipulation of the BSF genome to insert transgenes for expression of functional protein products.

Utilizing Cyclic Voltammetry to Understand the Energy Storage Mechanisms for Copper Oxide and its Graphene Oxide Hybrids as Lithium-Ion Battery Anodes.

Invited for this month's cover is the group of Robert A. W. Dryfe at the University of Manchester in collaboration with William Blythe Ltd. (Lancashire). The image shows bees building a graphene-containing battery that powers an external circuit, depicted by a purple curve. This curve represents a cyclic voltammogram, specifically of copper oxide during a charge/discharge cycle in a Li-ion half-cell, as presented in the article. The use of bees has both scientific and geographic significance. For the former, bees are assisting in building the honeycomb-like hexagonal graphene lattice, just as they do in nature. Geographically, bees are symbolic to the locations of both collaborators on this project, the University of Manchester and William Blythe (Lancashire). The Full Paper itself is available at 10.1002/cssc.201902784.

Utilizing Cyclic Voltammetry to Understand the Energy Storage Mechanisms for Copper Oxide and its Graphene Oxide Hybrids as Lithium-Ion Battery Anodes.

Graphene-based materials have been extensively researched as a means improve the electrochemical performance of transition metal oxides in Li-ion battery applications, however an understanding of the effect of the different synthesis routes, and the factors underlying the oft-stated better performance of the hybrid materials (compared to the pure metal oxides) is not always demonstrated. For the first time, we report a range of synthetic routes to produce graphene oxide (GO)-coated CuO, micro-particle/GO "bundles" as well as nano-particulates decorated on GO sheets to enable a comparison with CuO and its carbon-coated analogue, as confirmed using scanning electron microscopy (SEM) imaging and Raman spectroscopy. Cyclic voltammetry was utilized to probe the lithiation/delithiation mechanism of CuO by scanning at successively decreasing vertex potentials, uncovering the importance of a full reduction to Cu metal on the reduction step. The GO hybrid materials clearly show enhanced specific capacities and cycling stabilities comparative to the CuO, with the most promising material achieving a capacity of 746 mAh g-1 and capacity retention of 92 % after 30 cycles, which is the highest stable capacity quoted in literature for CuO. The simple cyclic voltammetry technique used in this work could be implemented to help further understand any conversion-type anode materials, in turn accelerating the research and industrial development of conversion anodes.

Identifying Chicago's High Users of Police-Involved Emergency Services.

OBJECTIVES: To identify individuals at risk for behavioral health (BH)-involved encounters with police in Chicago, Illinois. METHODS: We linked Chicago Police Department (CPD) arrest and Fire Department (CFD) BH-involved ambulance event data. We identified at-risk individuals who accumulated at least 1 BH-involved ambulance and at least 1 arrest event between May 2016 and April 2017. We identified a high-use subgroup displaying most intensive services use. We identified high-use locations with highest volume of ambulance events with only CFD data. RESULTS: Of 83 392 individuals and 116 105 events in the linked emergency events data, 1842 at-risk individuals accounted for 2.2% of individuals, 5.6% of all events, and 16% of BH-involved CFD events with police involvement. A total of 330 high-use individuals accounted for 0.4% of individuals, 2% of events, and 4.7% of CFD events with police involvement. Top-100 high-use locations accounted for 9% of CFD events, and individuals of high-use location events are largely distinct from high-use individuals. CONCLUSIONS: Integrated police and ambulance data hold promise to identify individuals at risk for BH-involved encounters with police and to support proactive interventions to prevent or improve response at these encounters.

The Neuronal Gene Arc Encodes a Repurposed Retrotransposon Gag Protein that Mediates Intercellular RNA Transfer.

The neuronal gene Arc is essential for long-lasting information storage in the mammalian brain, mediates various forms of synaptic plasticity, and has been implicated in neurodevelopmental disorders. However, little is known about Arc's molecular function and evolutionary origins. Here, we show that Arc self-assembles into virus-like capsids that encapsulate RNA. Endogenous Arc protein is released from neurons in extracellular vesicles that mediate the transfer of Arc mRNA into new target cells, where it can undergo activity-dependent translation. Purified Arc capsids are endocytosed and are able to transfer Arc mRNA into the cytoplasm of neurons. These results show that Arc exhibits similar molecular properties to retroviral Gag proteins. Evolutionary analysis indicates that Arc is derived from a vertebrate lineage of Ty3/gypsy retrotransposons, which are also ancestors to retroviruses. These findings suggest that Gag retroelements have been repurposed during evolution to mediate intercellular communication in the nervous system.

Arc: building a bridge from viruses to memory.

Arc (activity-regulated cytoskeleton-associated protein) is a neuron-specific immediate early gene that is required for enduring forms of synaptic plasticity and memory in the mammalian brain. Arc expression is highly dynamic, and tightly regulated by neuronal activity and experience. Local translation of Arc protein at synapses is critical for synaptic plasticity, which is mediated by Arc-dependent trafficking of AMPA (α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid)-type glutamate receptors. To date, few structural or biophysical properties of Arc protein have been investigated. Recent studies, including that of Myrum et al. published in the 468:1 issue of the Biochemical Journal, now shed light on some intriguing biophysical properties of Arc. These findings show that Arc contains large N- and C-terminal domains around a flexible linker region and that purified Arc protein is capable of self-oligomerization. Intriguingly, these domains show homology with the viral capsid protein found in the gag polypeptide of most retroviruses. These studies provide insight into how Arc may regulate multiple critical cell biological processes in neurons and reveals unanticipated biology that resembles viral trafficking in cells.

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

Amyotrophic Lateral Sclerosis (ALS) is a late-onset, progressive neurodegenerative disease affecting motor neurons in the brain stem and spinal cord leading to loss of voluntary muscular function and ultimately, death due to respiratory failure. A subset of ALS cases are familial and associated with mutations in superoxide dismutase 1 (SOD1) that destabilize the protein and predispose it to aggregation. In spite of the fact that sporadic and familial forms of ALS share many common patho-physiological features, the mechanistic relationship between SOD1-associated and sporadic forms of the disease if any, is not well understood. To better understand any molecular connections, a cell-based protein folding assay was employed to screen a whole genome RNAi library for genes that regulate levels of soluble SOD1. Statistically significant hits that modulate SOD1 levels, when analyzed by pathway analysis revealed a highly ranked network containing TAR DNA binging protein (TDP-43), a major component of aggregates characteristic of sporadic ALS. Biochemical experiments confirmed the action of TDP-43 on SOD1. These results highlight an unexpected relationship between TDP-43 and SOD1 which may have implications in disease pathogenesis.

Alcohol use by undergraduate students on their 21st birthday: predictors of actual consumption, anticipated consumption, and normative beliefs.

Recent research has identified celebration of a 21st birthday as an environmental event during which many college students engage in risky levels of alcohol consumption. The current study examined the relationship between personality and different aspects of alcohol use during 21st birthday celebrations: actual amount consumed for those who had turned 21, anticipated amount consumed for those under the age of 21, and normative beliefs regarding the amount other students consume on their 21st birthdays. Sensation seeking and impulsivity both displayed significant bivariate relationships with all three aspects of 21st birthday drinking. Personality traits did not contribute unique variance to actual 21st birthday drinking after the effects of typical alcohol consumption were accounted for in the models. Impulsivity contributed unique variance to models accounting for anticipated drinking and normative beliefs. Additional research is necessary to better understand the role personality variables play on alcohol consumption during 21st birthday celebrations.

Influence of prenatal stress on behavioral, endocrine, and cytokine responses to adulthood bacterial endotoxin exposure.

Prior research suggests that prenatal stress, among other effects, can lead to hyper-reactivity of the offspring's hypothalamic-pituitary-adrenal (HPA) axis and alterations in immune function. These stress-induced changes have been linked to a greater propensity to develop depression or anxiety disorders. Furthermore, prenatally stressed offspring may be more susceptible to certain diseases. The immune alterations induced by prenatal stress exposure may disrupt the normal communication between the immune system, endocrine system, and central nervous system, potentially making prenatally stressed individuals more vulnerable to the negative aspects of immune activation, including cytokine-induced cognitive deficits and anxiety. The present study investigated whether prenatal stress would exaggerate these detrimental effects of peripheral immune activation. We hypothesized that prenatally stressed subjects would be hypersensitive to endotoxin administration and would therefore show exaggerated learning deficits, increased anxiety-like behavior, and increased peripheral and central interleukin-1beta (IL-1beta) levels. The observed results only partially supported our hypotheses, as prenatally stressed subjects showed evidence, albeit modest, of increased anxiety-like behavior following endotoxin administration relative to non-stressed controls. While prenatal stress exposure or lipopolysaccharide (LPS) administration independently impaired learning, the data failed to support the hypothesis that prenatally stressed subjects would show exaggerated cognitive deficits, engendered via enhanced peripheral and central IL-1beta levels, following immune activation. Collectively, the data suggest that although prenatal stress exposure led to increases in anxiety-like behavior following endotoxin exposure, it did not appear to increase susceptibility to LPS-induced cognitive decline or elevations in proinflammatory cytokine production.