RESUMEN
In light of severe health risks of foodborne pathogenic bacterial diseases, the potential utility of point-of-care (POC) sensors is recognized for pathogens detection. In this regard, lateral flow assay (LFA) is a promising and user-friendly option for such application among various technological approaches. This article presents a comprehensive review of "lock-and-key" recognizer-encoded LFAs with respect to their working principles and detection performance against foodborne pathogenic bacteria. For this purpose, we describe various strategies for bacteria recognition including the antibody-based antigen-antibody interactions, nucleic acid aptamer-based recognition, and phage-mediated targeting of bacterial cells. In addition, we also outline the technological challenges along with the prospects for the future development of LFA in food analysis. The LFA devices built based upon many recognition strategies are found to have great potential for rapid, convenient, and effective POC detection of pathogens in complex food matrixes. Future developments in this field should emphasize the development of high-quality bio-probes, multiplex sensors, and intelligent portable readers.
Asunto(s)
Técnicas Biosensibles , Enfermedades Transmitidas por los Alimentos , Humanos , Técnicas Biosensibles/métodos , Enfermedades Transmitidas por los Alimentos/diagnóstico , Enfermedades Transmitidas por los Alimentos/microbiología , Bacterias , Bioensayo , Sistemas de Atención de PuntoRESUMEN
Portable devices for on-site foodborne pathogens detection are urgently desirable. Lateral flow immunoassay (LFIA) provides an efficient strategy for pathogens detection, however, antibody labeling independence and detection reliability, are still challenging. Here, we report the development of a label-free LFIA with dual-readout using glucan-functionalized two-dimensional (2D) transition metal dichalcogenides (TMDs) tungsten disulfide (WS2) as detection probes for sensitive detection of Salmonella enteritidis (S. enteritidis). In particular, glucan-functionalized WS2, synthesized via liquid exfoliation, are reliable detection antibody candidates which served as antibody mimics for bacteria capturing. This LFIA has not only eliminated the intricate antibody labeling process and screening of paired antibodies in conventional LFIAs, but also promised dual-readout (colorimetric/Raman) for flexible detection. Under optimized conditions, this LFIA achieves selective detection of S. enteritidis with a low visual detection limit of 103 CFU/mL and a broad linear range of 103-108 CFU/mL. Additionally, the LFIA could be successfully applied in drinking water and milk with recoveries of 85%-109%. This work is desirable to expand the application of 2D TMDs in biosensors and offers a brand-new alternative protocol of detection antibodies in foodborne pathogens detection.
Asunto(s)
Técnicas Biosensibles , Salmonella enteritidis , Reproducibilidad de los Resultados , Inmunoensayo/métodos , Anticuerpos , Límite de DetecciónRESUMEN
To explore the superiority of multifunctional nanocomposites and realize the joint-detection of foodborne pathogens, an immersible amplification dip-stick immunoassay (DSIA) was exploited for the sensitive detection of Salmonella typhimurium (S. typhi) and Escherichia coli O157:H7 (E. coli O157:H7). Saving for the basic colorimetric performance, the reporter molecule of CoFe2O4 (CFO) possesses multivalent elements (Co2+/3+, Fe2+/3+) as well as multifunction of superior catalase-like activity and magnetic properties. By dint of the catalytic activity of CFO, a directly immersible amplification can be simply achieved to endure the DSIA with an intensive signal and a dual-visible mode for the determination of S. typhi and E. coli O157:H7. In virtue of the magnetic separation and enrichment capability of the CFO, the DSIA can perform a matrix-interference-free detection and obtain a dynamic detection range of 102-108 CFU/mL and a low assay limit of 102 CFU/mL. Moreover, the DSIA has reasonable recovery rates for contamination monitoring of two target bacteria in milk and beef samples. Our research provides a persuasive supplement for the application of multifunctional nanocomposites in the ongoing dip-stick immunoassay and an alternative strategy for the efficient detection of foodborne pathogens.