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The high oil and salt content of kitchen waste (KW) inhibit bioconversion and humus production. To efficiently degrade oily kitchen waste (OKW), a halotolerant bacterial strain, Serratia marcescens subsp. SLS which could transform various animal fats and vegetable oils, was isolated from KW compost. Its identification, phylogenetic analysis, lipase activity assays, and oil degradation in liquid medium were assessed, and then it was employed to carry out a simulated OKW composting experiment. In liquid medium, the 24 h degradation rate of mixed oils (soybean oil: peanut oil: olive oil: lard = 1:1:1:1, v/v/v/v) was up to 87.37% at 30 °C, pH 7.0, 280 rpm, 2% oil concentration and 3% NaCl concentration. The ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS) method demonstrated that the mechanism of SLS strain metabolizing long-chain triglycerides (TAGs) (C53-C60), especially the biodegradation of TAG (C18:3/C18:3/C18:3) by the strain can reach more than 90%. Degradation of 5, 10, 15% concentrations of total mixed oil were also calculated to be 64.57, 71.25, 67.99% respectively after a simulated composting duration of 15 days. The results suggest that the isolated strain of S. marcescens subsp. SLS is suitable for OKW bioremediation in high NaCl concentration within a reasonably short period of time. The findings introduced a salt-tolerant and oil-degrading bacteria, providing insights into the mechanism of oil biodegradation and offering new avenues of study for OKW compost and oily wastewater treatment.
Asunto(s)
Serratia marcescens , Cloruro de Sodio , Serratia marcescens/metabolismo , Biodegradación Ambiental , Cromatografía Liquida , Filogenia , Cloruro de Sodio/metabolismo , Espectrometría de Masas en Tándem , Aceites de PlantasRESUMEN
QNZ is a quinazoline-type NF-κB inhibitor and is one of the hot anti-inflammatory drug candidates in recent years. With its development and application, QNZ will inevitably enter the aquatic environment posing a threat to aquatic organisms. To investigate the potential toxicity of QNZ in the early life stages of the organism, this study exposed embryos of large-scale loach (Paramisgurnus dabryanus) to 0, 20, 40, 60, and 80 nM of QNZ. The hatching of embryos was significantly inhibited and hatching time was delayed. We explored the mechanism of hatching delay and failure. The results suggested that QNZ exposure reduced the number of hatching gland cells (HGCs) and hatching enzyme activity. Also, the frequency of spontaneous movements was inhibited by interfering with the expression of genes related to the cholinergic system and skeletal muscle development. Further, QNZ exposure induces a series of morphological changes (spine deformation, pericardial edema, tail deformation, and yolk sac edema) in embryos and newly-hatched larvae, and finally increased the deformity rate and mortality rate of newly-hatched larvae. The information presented in this study will provide a scientific basis for further studies into the potential toxicity of QNZ on aquatic organisms.
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Cipriniformes , Animales , Saco Vitelino , Larva , Metaloendopeptidasas , Embrión no MamíferoRESUMEN
Pollution of microplastics (MPs) has become a global environmental issue due to the difficulty in its degradation and may cause unexpected ecological effects. Nevertheless, little is known about the potential effects of MPs on reproduction toxicity in aquatic species. In this study, adult loach (Paramisgurnus dabryanus, F0 generation) were exposed to two concentrations (1 and 10 mg/L) of polyethylene MPs (PE-MPs) for 15 or 30 days, and the toxic effects in parental loach and the offspring (F1 generation) were examined. Our results showed that PE-MPs exposure could change the indicators content of antioxidant system in the brain, liver, and gonad. PE-MPs can accumulate in the gonads, disrupt the transcription of HPG-axis related genes, alter sex hormone levels, increase cell apoptosis and gonadal pathological lesions, lead to the damage of biological characteristics of semen, and affect the reproduction in F0 generation. PE-MPs remaining in the parental gonads can be transferred to the F1 generation embryos and accumulated on the embryonic chorionic membrane, increasing mortality and malformation rates, accelerating hatching time, and decreasing hatching rate and body length. These results suggest that PE-MPs leads to a potential adverse influence on reproduction and serious impacts on population sustainability. This work provides a new perspective into the effects of MPs on reproductive damage and cross-generational effects in teleost fish, which have implications in fields of freshwater ecology and environmental toxicology.
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Microplásticos , Contaminantes Químicos del Agua , Animales , Microplásticos/toxicidad , Polietileno/toxicidad , Plásticos/toxicidad , Contaminantes Químicos del Agua/análisis , ReproducciónRESUMEN
Eco-friendly ethanol (EtOH)-water (H2O) mixture has demonstrated huge potential in the textile industry. However, the uncontrolled discharge of dye-contaminated EtOH-H2O mixture to the ecosystem has numerous adverse effects. Herein, a sustainable approach utilizing the agricultural waste biomass-Juncus effusus (JE) to synthesize magnetic cellulose JE powders (M-JEPs) has been proposed for purification of dye-contaminated EtOH-H2O mixture. Batch experiments and physical-chemical analyses were performed to explore the adsorption performance and mechanism. The as-prepared cellulose M-JEPs exhibited ultrafast adsorption performance, which can reach the adsorption equilibrium within 10 min. The adsorption isotherms and kinetics demonstrated that the adsorption fitted well with the Langmuir isotherm and pseudo-second-order kinetic models, exhibiting the maximum adsorption capacity towards C.I. Reactive Red 195 and C.I. Reactive Blue 222 of 58.21 mg/g and 86.06 mg/g at the temperature of 303K. These findings indicate the feasibility of using cellulose M-JEPs for rapid purification of the dye-contaminated EtOH-H2O mixture.
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Efforts to impart responsiveness to environmental stimuli in artificial hydrogel fibers are crucial to intelligent, shape-memory electronics and weavable soft robots. However, owing to the vulnerable mechanical property, poor processability, and the dearth of scalable assembly protocols, such functional hydrogel fibers are still far from practical usage. Herein, we demonstrate an approach toward the continuous fabrication of an electro-responsive hydrogel fiber by using the self-lubricated spinning (SLS) strategy. The polyelectrolyte inside the hydrogel fiber endows it with a fast electro-response property. After solvent exchange with triethylene glycol (TEG), the maximum tensile strength of the hydrogel fiber increases from 114 kPa to 5.6 MPa, far superior to those hydrogel fiber-based actuators reported previously. Consequently, the flexible and mechanical stable hydrogel fiber is knitted into various complex geometries on demand such as a crochet flower, triple knot, thread tube, pentagram, and hollow cage. Additionally, the electrochemical-responsive ionic hydrogel fiber is capable of acting as soft robots underwater to mimic biological motions, such as Mobula-like flapping, jellyfish-mimicking grabbing, sea worm-mimicking multi-degree of freedom movements, and human finger-like smart gesturing. This work not only demonstrates an example for the large-scale production of previous infeasible hydrogel fibers, but also provides a solution for the rational design and fabrication of hydrogel woven intelligent devices.
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Non-destructive rail testing and evaluation based on guided waves need accurate information about the mode propagation characteristics, which can be obtained numerically with the exact material properties of the rails. However, for rails in service, it is difficult to accurately obtain their material properties due to temperature fluctuation, material degradation and rail profile changes caused by wear and grinding. In this study, an inverse method is proposed to identify the material elastic constants of in-service rails by minimizing the discrepancy between the phase velocities predicted by a semi-analytical finite element model and those measured using array transducers attached to the rail. By selecting guided wave modes that are sensitive to moduli but not to rail profile changes, the proposed method can make stable estimations for worn rails. Numerical experiments using a three-dimensional finite element model in ABAQUS/Explicit demonstrate that reconstruction accuracies of 0.36% for Young's modulus and 0.87% for shear modulus can be achieved.
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Metal-organic frameworks (MOFs) with long-term stability and reversible high water uptake properties can be ideal candidates for water harvesting and indoor humidity control. Now, a mesoporous and highly stable MOF, BIT-66 is presented that has indoor humidity control capability and a photocatalytic bacteriostatic effect. BIT-66 (V3 (O)3 (H2 O)(BTB)2 ), possesses prominent moisture tunability in the range of 45-60 % RH and a water uptake and working capacity of 71 and 55â wt %, respectively, showing good recyclability and excellent performance in water adsorption-desorption cycles. Importantly, this MOF demonstrates a unique photocatalytic bacteriostatic behavior under visible light, which can effectively ameliorate the bacteria and/or mold breeding problem in water adsorbing materials.
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Antibacterianos/farmacología , Escherichia coli/efectos de los fármacos , Estructuras Metalorgánicas/farmacología , Vanadio/farmacología , Antibacterianos/química , Catálisis , Teoría Funcional de la Densidad , Humedad , Hidrólisis , Luz , Estructuras Metalorgánicas/química , Pruebas de Sensibilidad Microbiana , Tamaño de la Partícula , Procesos Fotoquímicos , Porosidad , Propiedades de Superficie , Vanadio/química , Agua/químicaRESUMEN
As a new type of jamming, the interrupted sampling repeater jamming (ISRJ) derived from the digital radio frequency memory (DRFM) technology, can generate coherent multiple false targets after pulse compression. At present, the traditional interference suppression method and its improved methods have insufficient characteristics and poor detection performance under the condition of low signal-to-noise ratio (SNR). Aiming at addressing this defect, this paper proposes an interference suppression method for ISRJ based on singular spectrum entropy function (SSEF) from the aspects of singular value decomposition (SVD) and information entropy theories. In this method, firstly, considering the local fine characteristics and extraction efficiency, an adaptive multi-scale segmentation (AMS) method is proposed. The purpose of this processing is to extend the salient characteristics while to smooth the similar ones. In AMS, the segmentation criterion based on average energy of segments and the constraint of minimum segmentation is also proposed, then the improved delay embedded matrix is established from the improved trajectory matrix by AMS and delay embedded mapping. Secondly, the singular spectrum of the improved delay embedded matrix is extracted by SVD. Thirdly, because the recognition algorithms based on singular spectrum analysis (SSA), classical SSE and other characteristics fail at low SNR, this paper proposes a characteristic named as SSEF retrieved from the Shannon entropy model. The following proposed entropy-based threshold detection is carried out on the echo signal to realize the band-pass filtering and interference suppression. Finally, experiment results show that in comparison with other interference suppression approaches, SSEF can increase the probability of target detection and the peak-to-side-lobe ratio (PSR) after pulse compression, which validates its stability to noise and jamming especially in the condition of low SNRs.