Effects of lexical skills and orthographic neighborhood size in word memory.

Two experiments were designed to investigate the relationship between individual lexical skills in young adults and memory performance on words varying by their orthographic neighborhood size. In Experiment 1, a sample of 100 university students were administrated a set of spelling, reading, and vocabulary tests to assess their lexical skills. Then, they had to learn mixed lists of words from high and low neighborhood size and perform free recall and memory recognition tasks. Importantly, high lexical skills were found to enhance free recall and, to a lesser extent, recognition. In addition, a typical mirror effect of neighborhood size was found in recognition as words were better recognized and also produced less false alarms when they had a low neighborhood size. In Experiment 2, pure lists of words were designed and a new sample of 90 university students was assessed. We replicated the effect of lexical skills in free recall and the effect of neighborhood size for hits in recognition. Spelling skills were found to interact with neighborhood size in free recall in that low spelling skills were associated with a facilitatory effect of neighborhood size. In recognition, a relation between reading skills and neighborhood size was found such that the higher the reading skills, the higher was the inhibitory effect of neighborhood size. These results provide new evidence of an influence of lexical skills in word memory performance and underline the role of orthographic neighborhood size in episodic memory tasks.

DMRT1 is a testis-determining gene in rabbits and is also essential for female fertility.

DMRT1 is the testis-determining factor in several species of vertebrates, but its involvement in mammalian testes differentiation, where SRY is the testis-determining gene, remains ambiguous. So far, DMRT1 loss-of-function has been described in two mammalian species and induces different phenotypes: Disorders of Sex Development (46, XY DSD) in men and male infertility in mice. We thus abolished DMRT1 expression by CRISPR/Cas9 in a third species of mammal, the rabbit. First, we observed that gonads from XY DMRT1-/- rabbit fetuses differentiated like ovaries, highlighting that DMRT1 is involved in testis determination. In addition to SRY, DMRT1 is required in the supporting cells to increase the expression of the SOX9 gene, which heads the testicular genetic cascade. Second, we highlighted another function of DMRT1 in the germline since XX and XY DMRT1-/- ovaries did not undergo meiosis and folliculogenesis. XX DMRT1-/- adult females were sterile, showing that DMRT1 is also crucial for female fertility. To conclude, these phenotypes indicate an evolutionary continuum between non-mammalian vertebrates such as birds and non-rodent mammals. Furthermore, our data support the potential involvement of DMRT1 mutations in different human pathologies, such as 46, XY DSD as well as male and female infertility.

Animals that reproduce sexually have organs called gonads, the ovaries and testes, which produce eggs and sperm. These organs, which are different in males and females, originate from the same cells during the development of the embryo. As a general rule, the chromosomal sex of an embryo, which gets determined at fertilization, leads to the activation and repression of specific genes. This in turn, controls whether the cells that will form the gonads will differentiate to develop testes or ovaries. Disruption of the key genes involved in the differentiation of the gonads can lead to fertility problems, and in some cases, it can cause the gonads to develop in the 'opposite' direction, resulting in a sex reversal. Identifying these genes is therefore essential to know how to maintain or restore fertility. DMRT1 is a gene that drives the differentiation of gonadal cells into the testicular pathway in several species of animals with backbones, including species of fish, frogs and birds. However, its role in mammals ­ where testis differentiation is driven by a different gene called SRY ­ is not well understood. Indeed, when DMRT1 is disrupted in male humans it leads to disorders of sex development, while disrupting this gene in male mice causes infertility. To obtain more information about the roles of DMRT1 in mammalian species, Dujardin et al. disrupted the gene in a third species of mammal: the rabbit. Dujardin et al. observed that chromosomally-male rabbits lacking DMRT1 developed ovaries instead of testes, showing that in rabbits, both SRY and DMRT1 are both required to produce testes. Additionally, this effect is similar to what is seen in humans, suggesting that rabbits may be a better model for human gonadal differentiation than mice are. Additionally, Dujardin et al. were also able to show that in female rabbits, lack of DMRT1 led to infertility, an effect that had not been previously described in other species. The results of Dujardin et al. may lead to better models for gonadal development in humans, involving DMRT1 in the differentiation of testes. Interestingly, they also suggest the possibility that mutations in this gene may be responsible for some cases of infertility in women. Overall, these findings indicate that DMRT1 is a key fertility gene.

Vocabulary and reading comprehension: what are the links in 7- to 10 year-old children?

The aims of the present study are: (1) to examine the contribution that vocabulary makes to reading comprehension in the Simple View of Reading model in French-speaking children aged from 7 to 10 years based on the use of an index of efficiency (i.e., speed-accuracy index); and (2) to investigate the extent to which the contribution of vocabulary to reading comprehension might change according to children's school grade level. Measures of vocabulary depth, word reading (i.e., three levels of word representations, namely orthography, phonology, semantics), listening, and reading comprehension were collected using computer-based assessments in children from Grades 2 to 5 (N = 237). We examined the contribution of vocabulary in two contrasted groups: a younger group consisting of children from Grades 2-3 and an older group with children from Grades 4-5. A confirmatory factor analysis revealed that vocabulary is a factor separate from word reading, listening and reading comprehension. Moreover, the results from a structural equation modeling analysis showed that word reading and listening comprehension fully mediated the relation between vocabulary and reading comprehension. Consequently, vocabulary had an indirect effect via word reading on reading comprehension in both groups. Finally, word reading had a greater effect on reading comprehension than listening comprehension in both groups. The results suggest that word reading plays a central role in reading comprehension and is underpinned by the influence of vocabulary. We discuss the results in the light of the lexical quality hypotheses taken together with reading comprehension.

Absence of Testicular Estrogen Leads to Defects in Spermatogenesis and Increased Semen Abnormalities in Male Rabbits.

Estrogens are steroid hormones produced by the aromatization of androgens by the aromatase enzyme, encoded by the CYP19A1 gene. Although generally referred to as "female sex hormones", estrogen is also produced in the adult testes of many mammals, including humans. To better understand the function of estrogens in the male, we used the rabbit model which is an important biomedical model. First, the expression of CYP19A1 transcripts was localized mainly in meiotic germ cells. Thus, testicular estrogen appears to be produced inside the seminiferous tubules. Next, the cells expressing ESR1 and ESR2 were identified, showing that estrogens could exert their function on post-meiotic germ cells in the tubules and play a role during sperm maturation, since ESR1 and ESR2 were detected in the cauda epididymis. Then, CRISPR/Cas9 CYP19A1-/- genetically modified rabbits were analyzed. CYP19A1-/- males showed decreased fertility with lower sperm count associated with hypo-spermatogenesis and lower spermatid number. Germ/sperm cell DNA methylation was unchanged, while sperm parameters were affected as CYP19A1-/- males exhibited reduced sperm motility associated with increased flagellar defects. In conclusion, testicular estrogens could be involved in the spermatocyte-spermatid transition in the testis, and in the acquisition of sperm motility in the epididymis.

Norms of vocabulary, reading, and spelling tests in French university students.

The purpose of the present paper is to provide the norms of widely used tests of vocabulary, reading, and spelling skills for a French population of young adults. Data were collected from 18- to 26-year-old university students during individual sessions. We assessed 771 participants with the Mill Hill part B vocabulary test, 410 with the LexTale-Fr test, 1231 with the Alouette-R test, and 361 with the Pollueur word/pseudoword dictation and text dictation. Stepwise regression analyses showed the need to stratify the reference population according to the level of education and gender for some tests. The Alouette-R and Mill Hill scores increased with educational level. Moreover, for the tests whose performance differed according to gender, women generally performed better than men. The present normative data concerning vocabulary, reading, and spelling skills should provide useful tools for researchers and practitioners alike to rate young individuals within their reference population.

Effects of deletion neighbourhood frequency and individual differences in lexical decision, progressive demasking, and naming.

This study investigates whether deletion neighbourhood frequency influences the processing of French written words, and whether it might also be influenced by individual differences in skilled adult readers. For this purpose, words with at least 1 higher-frequency deletion neighbour (e.g., pliage [folding]/plage [beach]) and others with no higher-frequency neighbour (e.g., morose [gloomy]) were presented in lexical decision (Experiment 1), progressive demasking (Experiment 2), and naming (Experiment 3) tasks. For each experiment, the participants' lexical skills were assessed by spelling, reading, and vocabulary tests. In Experiments 1-3, participants responded more slowly to words with at least 1 higher-frequency deletion neighbour than they did to words with no such neighbour. We also found evidence that the inhibitory effect of deletion neighbourhood frequency was sensitive to lexical skills in the naming task. These findings are discussed in terms of lexical competition underlying visual word recognition according to individual differences. (PsycInfo Database Record (c) 2020 APA, all rights reserved).