In vitro differentiation induction of embryonal carcinoma stem cells into insulin-producing cells by Cichorium intybus L. leaf extract.

ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal herb Cichorium intybus L. (chicory) has been used traditionally for the treatment of various diseases, including diabetes. One of the promising therapeutic options to treat diabetes is replacing the degenerative pancreatic ß cells by stem cell-derived IPCs (insulin-producing cells). AIM OF THE STUDY: By the combination of cell therapy as a modern approach and traditional medicine, the current study was designed to evaluate the effects of chicory leaf extract (LE) on the differentiation potential of P19 EC cells (an embryonal carcinoma stem cell line) into IPCs. MATERIALS AND METHODS: The plant (voucher no. 4567) were collected and deposited in the herbarium of Shahrekord University. In vitro experiments were designed to compare the effects of various concentrations of LE on the differentiation potential of P19 EC cells. RESULTS: The differentiated cells showed morphological characteristics of pancreatic ß cells. They could also synthesized and secreted insulin when exposed to glucose. Moreover, the cells expressed specific proteins and genes of mature pancreatic ß cells. CONCLUSIONS: In conclusion, LE as a natural herbal extract was efficiently able to induce the differentiation of P19 EC cells into the clusters similar to pancreatic islets with the molecular, cellular and functional characteristics of mature ß cells.

Understanding transport mechanisms in ionic liquid/carbonate solvent electrolyte blends.

To unravel mechanistic details of the ion transport in liquid electrolytes, blends of the ionic liquid (IL) 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide (Pyr14TFSI), ethylene carbonate (EC) and dimethyl carbonate (DMC) with the conducting salts lithium hexafluorophosphate (LiPF6) and lithium bis(trifluoromethylsulfonyl)imide (LiTFSI) were investigated as a function of the IL concentration. Electrochemical impedance, Pulsed Field Gradient Nuclear Magnetic Resonance (PFG NMR) and Raman spectroscopy supported by Molecular Dynamics (MD) simulations allowed the structural and dynamic correlations of the ion motions to be probed. Remarkably, we identified that though the individual correlations among different ion types exhibit a clear concentration dependence, their net effect is nearly constant throughout the entire concentration range, resulting in approximately equal transport and transference numbers, despite a monitored cross-over from carbonate-based lithium coordination to a TFSI-based ion coordination. In addition, though dynamical ion correlation could be found, the absolute values of the ionic conductivity are essentially determined by the overall viscosity of the electrolyte. The IL/carbonate blends with a Pyr14TFSI fraction of ∼10 wt% are found to be promising electrolyte solvents, with ionic conductivities and lithium ion transference numbers comparable to those of standard carbonate-based electrolytes while the thermal and electrochemical stabilities are considerably improved. In contrast, the choice of the conducting salt only marginally affects the transport properties.

Data-entropy analysis of renal transplantation data.

INTRODUCTION: The terms entropy and robustness are currently used by biomedical investigators to predict the risk of change in a system. The former is the mathematical identification of uncertainty about a system, while the latter is the likelihood of system stability. We conducted an entropy-based analysis of our renal transplantation data set. MATERIALS AND METHODS: The input variables in our model included donors and recipients, past medical history, and other clinical data. The output variables were 6- month, 1-year, and 2- year patient and graft survivals. Data-entropy analysis was performed with Ontonix s.r.l. software (www.ontonix.com). RESULTS: The total input and output entropy was 13.14 and 1.54, respectively. The mean input and output robustness was 39.14% and 29.54%. The robustness amplification index was 0.75. The minimum entropy of the input variables was reported for a history of myocardial infarction (0.07), vascular disease (0.1), bladder residual (0.13), or urologic surgery (0.15). The minimum entropy of the output variables was 0.20 for 6-month patient survival; 0.22 for 1-year patient survival; 0.25 for 6-month graft survival; 0.27 for 1-year graft survival; 0.28 for 2-year patient survival; and 0.32 for 2-year graft survival. CONCLUSION: Data-entropy analysis demonstrated a high stability of our transplantation data set. Nevertheless, long-term outcomes, especially those of graft survival, were slightly more unpredictable.

Medical comorbidities after renal transplantation.

BACKGROUND: Despite the amount of evidence regarding the negative impact of medical comorbidities after transplantation, little attention has been directly paid to the pattern of somatic comorbidities in renal transplant recipients. The aim of this study was to assess the prevalence of medical comorbidities after kidney transplantation. METHODS: In a cross-sectional study during 2006, we evaluated 119 kidney transplant recipients for somatic comorbidities by using the Ifudu comorbidity index, which evaluated the presence of 14 chronic illnesses among patients undergoing maintenance hemodialysis. Correlations of the Ifudu score with demographic and clinical data were also studied. RESULTS: Eighty-three (90.4%) subjects had at least one medical comorbidity. The mean comorbidity score was 5.17 +/- 4.50. The most frequent comorbidities were nonischemic heart diseases including hypertension (n=75; 63%), visual disturbances (n=42; 35.2%), low back pain and spine and joint disorders (n=30; 25.21%), and musculoskeletal disorders (n=28; 23.5%). A higher comorbidity score was significantly correlated with lower economic status (P<.05), but not with age, gender, marital status, educational level, cause, or duration of end-stage renal disease. CONCLUSION: The prevalence of medical comorbidities among kidney transplant recipients seems to be high, with the highest prevalence due to nonischemic heart diseases, visual disturbances, and musculoskeletal disorders. This highlighted the necessity of providing posttransplant care by a multidisciplinary team of specialists.

Anxiety and depression are correlated with higher morbidity after kidney transplantation.

BACKGROUND: Psychiatric comorbidities have been reported to be associated with low quality of life, but less attention has been paid to their impact on other morbidity measures. The aim of this study was to investigate the correlation of anxiety and depression with marital relation, sexual function, and sleep quality in kidney transplant recipients. METHODS: In a cross-sectional study between 2005 and 2006, 88 kidney transplant recipients were divided into four groups according to their scores of anxiety and depression using Hospital Anxiety Depression Scale (HADS): group I(anx) (anxiety score <11; n=64); group II(anx) (anxiety score >or= 11; n=24); group I(dep) (depression score <11; n=68); and group II(dep) (depression score >or= 11; n=20). Morbidity measures including quality of life (Short Form-36), marital adjustment (Revised Dyadic Adjustment Scale), sexual relationship (Relationship and Sexuality Scale), and quality of sleep (Pittsburgh Sleep Quality Index) were separately compared between groups of anxious versus nonanxious and depressed versus nondepressed. RESULTS: Group I(anx), compared with group II(anx), displayed a better state of mental health (48.80 +/- 7.14 vs. 44.45 +/- 7.80; P=.01), general health (49.36 +/- 12.77 vs. 42.91 +/- 16.67; P=.05), marital adjustment (55.13 +/- 8.01 vs. 48.35 +/- 16.62; P=.04), and lower sleep disturbance (1.36 +/- 0.62 vs. 1.66 +/- 0.63; P=.05). Group I(dep), compared with group II(dep), showed lower fatigue score (39.79 +/- 8.30 vs. 46.84 +/- 8.85; P=.002) and better sexual relationships (15.28 +/- 5.50 vs. 19.00 +/- 5.92; P=.03). CONCLUSIONS: Screening for anxiety and depression in kidney transplant recipients is essential. Appropriate treatment of these prevalent psychiatric comorbidities may improve various aspects of patient well-being, including quality of life, sleep, marital relations, and sexual relationship.