Therapeutic activity of sarpogrelate and dopamine D2 receptor agonists on cardiovascular and renal systems in rats with alloxan-induced diabetes.

BACKGROUND: Dopamine D2 receptor agonists, bromocriptine and cabergoline, are notable medications in the treatment of Parkinsonism, hyperprolactinemia, and hyperglycemia. An affiliation was found between the initiation of myocardial injury ailment and long term treatment with dopamine D2 agonist drugs identified with the partial activation of 5-hydroxytryptamine receptor 2 A (5-HT2A). The investigation aimed to examine the activity of sarpogrelate (a 5-HT2A receptor blocker) in reducing myocardial injury prompted by extended haul utilisation of D2 receptor agonists in rats with alloxan-induced diabetes. METHODS: Both bromocriptine and cabergoline were managed independently and combined with sarpogrelate for about a month in diabetic nephropathy rats. Both tail-cuff blood pressure and the BGL were recorded weekly. For all animals, the kidney hypertrophy index, serum creatinine, blood urea nitrogen, alanine transaminase, and aspartate transaminase levels were measured after one month of treatment. The severity of the cardiac injury was assessed by the estimation of lactate dehydrogenase-1 (LDH-1), cardiac troponin I, and tumor necrosis factor alpha 1 (TNF1). The triphenyltetrazolium chloride (TTC) staining method was used to determine the experimental myocardial infarction (MI) size. RESULTS: Bromocriptine and cabergoline created a significant reduction in BGL, BP, and kidney hypertrophy index in diabetic nephropathy rats. Administration of bromocriptine and cabergoline, alone, or in combination with sarpogrelate fundamentally diminished the blood concentrations of alkaline phosphatase (ALP), Aspartate aminotransferase (AST), urea, and creatinine. Bromocriptine and cabergoline alone showed a noteworthy increase in the LDH-1, Troponin I, and TNF1 levels in the serum (p < 0.05). Paradoxically, utilising bromocriptine or cabergoline with sarpogrelate treatment altogether decreased the levels of the myocardial biomarkers in the serum. A mix of bromocriptine or cabergoline with sarpogrelate diminished the level of the myocardial infarct size in the heart assessed through the TTC staining method. CONCLUSIONS: The examination demonstrated that the combined use of sarpogrelate with bromocriptine or cabergoline decreased the potential adverse effects of these two drugs on the myocardial tissues.

Genotypic and Environmental Variations in Kernel Color Indices in the Main Almond (Prunus dulcis (Mill.) D.A. Webb) Cultivars Grown in North-Eastern Morocco.

Color indices are important quality traits that define the consumer visual acceptance and agroindustrial preferences. Little is known regarding these properties in the commercial almond cultivars grown in Morocco. This work aimed at investigating kernel color indices in five cultivars, namely, "Fournat de Brézenaud," "Tuono," "Ferragnès," "Ferraduel," and "Marcona." Color indices consisted in the following: brightness (L ∗), redness index (a ∗), yellowness index (b ∗), chroma (C ∗), hue (H ∗), and metric saturation (S ∗). Measurements were performed over three consecutive growing seasons (2016-2018) across five different sites from northern (Aknoul, Bni Hadifa, and Tahar Souk) and eastern (Rislane and Sidi Bouhria) Morocco. All factors (cultivar, growing season, and site) affected significantly studied color properties; however, genotype was the main variability source. Wide variabilities were found among cultivars. "Marcona" showed the highest L ∗, while "Ferragnès" and "Ferraduel" displayed greater scores of a ∗, b ∗, C ∗, and S ∗. Sidi Bouhria presented the lowest L ∗ but higher a ∗, H ∗, and S ∗. Moreover, Bni Hadifa displayed higher L ∗, b ∗, and C ∗. 2016 (drier growing season) had the highest values of most indices. Principal component analyses (PCA) discriminate all factors through the first three components: PC1 (61%, genetic component) and PC2 (30%) and PC3 (7%) which were of environmental nature since they separate sites and growing seasons, respectively. Despite environmental effects, we suggested a possible discrimination among the studied cultivars based on their kernel color indices. Drought conditions during fruit development seemed to improve kernel quality via synthesis of pigments resulting in higher a ∗ and b ∗.

Physicochemical and microbiological characterization of olive mill wastewater (OMW) from different regions of northern Morocco.

This work examined the influence of crop season, extraction system and production site on the composition of fresh olive mill wastewater collected from northern Morocco. ANOVA analysis showed that most of the traits were largely affected by the extraction system (≥60% of total variance). Comparison among extraction systems showed significant differences for all traits, except for chlorides. Three phase centrifugation system (C3) displayed the highest value of total phenols, while all the remaining traits were higher in two phase centrifugation system (C2). Concerning crop seasons, we noticed a general decrease in values of total solids, chemical oxygen demand, chlorides, C/N ratio and total aerobic mesophilic flora, from 2014 to 2016 season; in contrast, an increase in suspended solids, total kjeldahl nitrogen and proteins was detected. OMW with highest total phenols content and then more acidic were collected during 2015 season. Between production sites, Bni Frassen produced OMW with the highest values for salt and total phenols, but the lowest ones were obtained for microbiological traits. OMW from Taza were rich in total kjeldahl nitrogen and proteins. An increase in organic load indicators was observed in Bouchfaa. Principal component analysis (PCA) explained about 82% of total variability: 58%, 16% and 8% for principal component 1 (PC1), PC2 and PC3, respectively. Extraction system fitted the variability of PC1. PC2 was mainly explained by production sites, while PC3 separated crop seasons. Correlation studies showed significant relationships especially among the traits expressing organic load, while microbiological counts were largely associated to total phenols and pH.