Dataset of 3D computer models of Late Miocene Mount Messenger Formation outcrops in New Zealand, built with UAV drones.

The aim of constructing 3D computer models of outcrops of the Mount Messenger Formation using unmanned aerial vehicle (UAV) drone technology was to enable better visualization and potential for analysis of deep-water sedimentary systems in Taranaki Basin, New Zealand. The Late Miocene-aged strata crop out along the north Taranaki coast of western North Island, New Zealand. The Mount Messenger Formation sandstone and siltstone beds are outstanding examples of deep-water sedimentary strata. These strata can be observed in outcrop sections, as well as in offshore drillholes (wireline logs) and in seismic reflection data acquired immediately offshore of the north Taranaki coastal section. In previous research undertaken on the Mount Messenger Formation in North Taranaki Basin, geologists used photographs and coupled these with observations and descriptions of strata in the field. Modern UAV drone technology now enables 3D perspectives to be obtained of outcrop sections, which greatly improves geometrical analysis of the rocks. This type of analysis, coupled with mapping of seismic reflection data in the immediate offshore area has enabled us to better understand the nature of Mount Messenger Formation deep-water sedimentary strata and to interpret the associated paleogeography with implications for energy resource exploration and evaluation. Using UAV drone photogrammetry, we acquired ∼3000 images of the Mount Messenger Formation outcrop at four locations along the north Taranaki coast. Drone surveys were conducted using a real-time kinetic (RTK) global positioning system (GPS) for accurate geolocation. The surveys were conducted on a DJI Phantom 4 drone, with a focal length of 24 mm with a 20-megapixel resolution. Survey images overlapped by 80-90%. The drone work adhered to the rules and regulations of the Aviation Security Service and the University of Waikato, New Zealand. Images were captured using programmed flight paths where the drone faced the outcrops at distances ranging from ∼3-7 m. 3D computer models were constructed using Pix4Dmapper version 4.4.12 to generate dense 3D point clouds, digital surface models (DSMs), triangle meshes, and orthomosaic images of the outcrops (i.e., 3D models). Once the 3D computer models of the outcrops were constructed, they were exported out of Pix4Dmapper as ArcGIS Scene Layer Package format (.slpk) and loaded into ArcGIS Pro version 3.0.3 for further analysis. The 3D computer models comprise a rich and valuable scientific dataset that can enhance geological analysis of sedimentary strata beyond the capabilities of photographs and manual fieldwork. These models allow desktop analysis of the geology and "virtual fieldwork" by imaging areas that are commonly inaccessible on foot due to their high elevation above ground level, location in rugged and steep terrane, as well as periodic intertidal flooding. This electronic geological dataset is stored in commonly used spatial format and plain-text ASCII files, allowing the preservation of geological data in digital records, especially when the outcrops are prone to erosion and cover by vegetation. The drone model dataset can be reused by the scientific community for virtual geological fieldwork, as petroleum and water reservoir analogues, as well as for research on coastal, environmental and geotechnical topics.

Evaluation the oral hygiene conditions, oral Candida colonization and salivary Streptococcus mutans and Lactobacilli density in a group of ß-thalassemic children and adolescence.

BACKGROUND: In this study, the prevalence and distribution of dental caries and oral hygiene conditions in a group of patients with ß-TM are evaluated and the results compared to age-and gender-matched healthy patients. In addition, oral candida colonization and the density of Streptococcus mutans (S.mutans) and Lactobacilli in the total saliva are assessed. MATERIAL AND METHODS: This study involved 59 ß-TM patients between 6-16 years old (mean:11.59±3.22), who applied to the Department of Pedodontics, Faculty of Dentistry, Akdeniz University, with ongoing follow-up, treatment and regular blood transfusions. All enrolled patients were diagnosed with ß-TM by the Department of Pediatric Hematology and Oncology, Faculty of Medicine, Akdeniz University. As a control group, age-and gender-matched healthy 50 patients were included to the study. RESULTS: Plaque ( p=0.001), DMFT ( p=0.009) and DMFS ( p=0.039) indices were significantly higher in the ß-TM patients, whereas, the oral hygiene status was significantly lower ( p=0.004). Saliva buffering capacity average was insignificantly but slightly more in ß-TM patients( p=0.131). While S.mutans values were significantly higher in the ß-TM patients ( p=0.002), no significant difference was found in the Lactobacillus ( p=0.131) and Candida values ( p=0.33). CONCLUSIONS: DMFT, DMFS, Plaque and oral hygiene indices and S.mutans values were found significantly different in ß-TM patients than healthy, control group patients, in this study.

The association of vascular endothelial growth factor, metalloproteinases and their tissue inhibitors with cardiovascular risk factors in the metabolic syndrome.

OBJECTIVE: The present study was proposed to examine the matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) and vascular endothelial growth factor (VEGF) in patients with metabolic syndrome (MetS), and to compare these parameters with healthy controls. We also compared the possible association of the circulating levels of MMP-2, MMP-9, TIMP-1, TIMP-2, and VEGF with cardiovascular risk factors in patients with MetS. We also compared the possible association of the circulating levels of MMP-2, MMP-9, TIMP-1, TIMP-2, and VEGF with cardiovascular risk factors in patients with MetS. PATIENTS AND METHODS: A total of 45 patients with MetS and 17 healthy controls with a body mass index (BMI) less than 25 kg/m2 were enrolled in the study. Plasma MMP-2, MMP-9, TIMP-1, TIMP-2, and VEGF levels were determined using ELISA. RESULTS: TIMP-1,-2, MMP-2,-9 levels were significantly higher in patients with MetS compared with healthy controls (p < 0.001). Carotid intima-media thickness and serum VEGF levels were found to be significantly increased (p < 0.01, p < 0.05 respectively) in MetS compared with healthy controls. According to the ROC curves, TIMP-1 levels were both sensitive (93.3%) and specific (81.2%). CONCLUSIONS: We observed that the patients with MetS have increased circulating concentrations of MMP-9, MMP-2, and TIMP-1, TIMP-2 that are associated with increased concentrations of VEGF. These findings suggest that MMP-2 may have a role in the increased cardiovascular risk of MetS patients.

Effects of chito-oligosaccharides and L-carnitine supplementation in diets for Japanese quails on performance, carcass traits and some blood parameters / Efeitos da chito-oligossacarídeos e suplementação de L-carnitina em dietas para codornas Japonesas sobre o desempenho, características de carcaça e de alguns parâmetros sanguíneos

The aim of this study was to determine effects of dietary supplementation with chitosanoligosaccharides (COS) and L-carnitine, individually or dually, on growth performance, carcass traits and some blood serum parameters in quails. A total of 192, four days old, Japanese quail chicks were allotted four groups, each of which included four replicates (12 birds per replicate). The groups received the same basal diet supplemented with 0 (Control), 150mg/kg chitosanoligosaccharides (COS), 150mg/kg L-carnitine (Carnitine), and 150 mg/kg chitosanoligosaccharides+150 mg/kg L-carnitine (COS+Car.) during the starter (1 to 21 days) and a grower (22 to 42 days) period. The feeding trial shoved that COS, L-carnitine and COS+L-carnitine had no significant effect on live weight, live weight gain, feed consumption and feed conversion. Supplementation with COS+L-carnitine induced higher leg ratio from than that of the Control. There were no differences on serum albumin, total protein, glucose and total cholesterol concentrations. It is concluded that due to the obtained higher leg ratio from COS+Car. group, after analysis of the profit and loss, if is economically profitable, chitosanoligosaccharides+L-carnitine could be added quail diets.

O estudo objetivou determinar os efeitos da suplementação com chito-oligossacarídeos (COS) e L-carnitina, individualmente ou em conjunto, sobre o desempenho, características de carcaça e alguns parâmetros sanguíneos em codornas. Um total de 192 codornas japonesas, com quatro dias de vida foi separado em quatro grupos, cada grupo com quatro repetições (12 aves por repetição). Os grupos receberam a mesma dieta basal suplementada com 0 (Controle), 150mg/kg chito-oligossacarídeos (COS), 150mg/kg L-carnitina (Carnitina), e 150mg/kg chito-oligossacarídeos +150 mg/kg L-carnitina (COS+Car.) durante o período inicial (1 a 21 dias) e de crescimento (22 a 42 dias). A fase de alimentação mostrou que COS, L-carnitina e COS+L-carnitina não tiveram efeito significativo no peso vivo, ganho de peso vivo, consumo de alimento e conversão de alimento. A suplementação com COS+L-carnitina induziu proporção de perna maior que o Controle. Não houve diferenças na concentração de albumina sérica, proteína total, glicose e colesterol total. Conclui-se que devido à proporção maior de perna obtida para o grupo COS+Car., após análise de perda e ganho, se for economicamente viável chito-oligossacarídeos+L-carnitina pode ser adicionado à dieta de codornas.

Endothelial damage in white coat hypertension: role of lectin-like oxidized low-density lipoprotein-1.

The aims of this study included an examination of soluble lectin-like oxidized low-density lipoprotein (LDL) receptor-1 (sLOX-1) levels in hypertensive (HT) patients. Another aim examined sLOX-1 associations with oxidized LDL (oxLDL), nitric oxide synthase (eNOS) and nitric oxide (NOx). A final aim was to compare these parameters between HT patients, white-coat hypertensive (WCH) patients and healthy controls. The three groups, HT, WCH and controls, were comprised of 35 patients each. sLOX-1 and oxLDL levels were significantly increased in WCH and HT patients compared with controls. The eNOS activation was significantly lower in HT than in the control group. sLOX-1 and oxLDL levels were significantly negatively correlated with eNOS levels in the WCH and HT groups. Carotid intima-media thickness (CIMT) measurements were significantly higher in the WCH and HT groups compared with controls. There was a significant positive correlation between CIMT and sLOX-1 and oxLDL; however, there was a negative correlation with eNOS in WCH. Regression analysis revealed that sLOX-1 was the variable that had a significant effect on blood pressure (P<0.001, odds ratio (95% confidence interval=23.273 (5.843-92.688)). A possible endothelial impairment may act as a cardiovascular risk factor in WCH. Necessary measures should be considered in terms of atherosclerosis risk with HT, especially in early identification of endothelial damage by looking at sLOX-1 levels. We believe sLOX-1 levels are strong biomarkers for determining early endothelial damage in HT, and especially in WCH patients.

Comparison of maternal and umbilical cord blood soluble lectin-like oxidized low-density lipoprotein receptor 1 levels in early- and late-onset preeclampsia.

BACKGROUND: The main purpose of this study was to determine the maternal and umbilical cord blood oxidized LDL (oxLDL) and soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in early- and late-onset preeclampsia (PE). MATERIALS AND METHODS: A case-control study was conducted in pregnant women with early-onset (before 34 weeks' gestation n = 19) and late-onset (after 34 weeks' gestation n = 22) PE compared to healthy normotensive pregnant controls (n = 44). Groups were compared for the maternal and umbilical cord plasma oxLDL and serum sLOX-1 levels. RESULTS: The mean maternal and umbilical cord serum sLOX-1 and plasma oxLDL levels were significantly increased in early- and late-onset PE compared to controls (p < 0.001). When early- and late-onset PE women were compared with serum sLOX-1 levels, the increase was more pronounced in early PE (p < 0.001). However, same comparison is not statistically significant in cord blood for oxLDL where as it is significantly higher in maternal blood for oxLDL in early-onset PE group. Maternal and cord blood oxLDL and sLOX-1 levels are positively correlated with each other; however, they are negatively correlated with fetal weight and gestational age. CONCLUSIONS: According to our results, maternal and umbilical cord blood levels of oxLDL and sLOX-1 were higher in preeclamptic pregnant. Thus, for the first time it has been shown that oxLDL and sLOX-1 levels were higher in fetal circulation as well as plasma of preeclamptic pregnant. However, sLOX-1 levels seem to be more implying than oxLDL for the differentiation of early and late preeclampsia.

Relation of protein oxidation parameters and disease activity in patients with Behçet's disease.

BACKGROUND: Behçet's disease (BD) is a chronic inflammatory vasculitis characterized by endothelial dysfunction, elevated reactive oxygen species (ROS), and neutrophil hyperfunction production including acute attacks and remission periods. Ischemia modified albumin (IMA), advanced oxidation protein products (AOPP), prooxidants-antioxidants balance (PAB), and ferric reducing antioxidant power (FRAP) were evaluated in regard to their role in the pathogenesis of BD as well as their relation to clinical presentation, uveitis attacks and remission periods, and healthy volunteers. METHODS: The study included 28 BD cases and 27 healthy volunteers as the control group. Blood samples were taken twice from each patient; first during an attack and second about three months after an attack, during remission period. RESULTS: AOPP, IMA and PAB levels were significantly increased in active periods of patients with BD compared with healthy control and remission periods of patients with BD (p < 0.0001, p < 0.0001, p < 0.0001, respectively). FRABP levels were found to be lower in active periods of patients with than healthy controls and remission periods of patients with BD (p < 0.001, p < 0.05, respectively). The AOPP levels were negatively correlated with the levels of FRAB in patients (r = -0.468, p = 0.012; r = -0.394, p = 0.038, respectively). The PAB levels were positively correlated with the levels of CRP in patients (r = -0.606, p = 0.001). CONCLUSIONS: Our results show that these parameters play a major role in the inflammatory reactions observed in BD. Increased levels of IMA and PAB are likely to be a result of inflammation-induced oxidative stress and hence its potential significance as a new marker of oxidative stress in BD.

Maternal and cord blood apelin, resistin and visfatin levels in gestational diabetes mellitus.

AIM: The aim of this paper was to investigate maternal and cord blood apelin, resistin and visfatin concentrations in pregnant women with and without gestational diabetes mellitus (GDM). METHODS: This case-control study was conducted on 24 women with GDM and 21 women without GDM. Maternal plasma and cord blood apelin, resistin and visfatin levels were measured with ELISA. RESULTS: The cord blood apelin levels were significantly lower in women with GDM than control subjects (111.23±31.53 vs.. 257.48±133.97 pg/mL, P=0.002). However, the decrease of maternal apelin levels in GDM group was not statistically significant (140.76±48.38 vs. 163.53±91.12 pg/mL, P=0.602). Women with GDM had lower maternal and cord blood visfatin concentrations and higher resistin concentrations than control group. Maternal resistin concentrations were significantly correlated with HOMA-IR (r=0.745, P=0.005). The apelin and visfatin levels did not correlate with HbA1c, BMI, HOMA-IR, glucose and birth weight. CONCLUSION: GDM is associated with lower cord blood apelin levels than control subjects. GDM appears to influence fetoplacental apelin metabolism. Apelin may not be directly involved in the regulation of maternal insulin sensitivity. Our results indicate that there is an increase in resistin concentrations and a decrease in visfatin concentrations in maternal serum and cord blood serum with GDM.

The role of feed regulating peptides on weight loss in patients with pulmonary tuberculosis.

PURPOSE: Malnutrition is a prominent feature of tuberculosis (TB). The aim of our study was to explore the function of plasma regulatory proteins in pulmonary TB and to investigate the relationship between these parameters and loss of body weight. METHODS: Plasma levels of fasting insulin, leptin, ghrelin, adiponectin and orexin-A were measured in 23 pulmonary TB patients, 39 patients with pulmonary sarcoidosis, 22 patients with different diffuse interstitial lung diseases and 21 healthy patients serving as controls. RESULT: Plasma leptin (p<0.001) and orexin-A (p<0.01) levels were significantly decreased in TB patients compared with those of the other study subjects. TB patients also had higher levels of plasma ghrelin compared with those of the other study subjects, while sarcoidosis patients had higher plasma adiponectin levels than the other study subjects. Glucose levels were similar in all groups, yet, insulin and Homeostasis Model of Assessment-Insulin Resistance (HOMA-IR) levels were significantly higher in the TB group compared to the other study groups. There was no correlation between leptin, ghrelin, adiponectin and orexin-A and other parameters. CONCLUSIONS: These data suggest that leptin and orexin-A levels have effects on weight loss in patients with pulmonary tuberculosis. Particularly, leptin may play a role in the early immune response to pulmonary TB and prolonged inflammation may further suppress leptin production. Measurement of HOMA-IR can indeed be used as a marker for the risk of activated TB. Further clinical studies are needed to better understand the role of feed regulating proteins in pulmonary tuberculosis.

Evaluation of serum paraoxonase and arylesterase activities in subjects with asthma and chronic obstructive lung disease.

BACKGROUND: Asthma and chronic obstructive lung disease (COPD) are characterised by airway inflammation. Paraoxonasel (PON1) and arylesterase (AE) enzymes have the ability to protect HDL from oxidation and may have antiatherogenic, antioxidant, and antiinflammatory features. We carried out a study to assess if there is a difference between PON1 and AE activities and biochemical values between asthmatics and COPD patients and if there is a difference between comorbid or pure COPD patients. METHODS: 40 asthmatics, 20 pure COPD, 20 comorbid COPD patients, and 20 healthy controls were included. We excluded patients with hypertension, metabolic syndrome, diabetes mellitus, thyroid, renal, hepatic, rheumatic, cardiac, cerebrovascular, malignant, and infectious diseases to establish the asthma and pure COPD groups. Patients using drugs which could affect PON1 and AE were excluded in these groups. There were 11 hypertensive, 5 diabetic, and 4 cardiac patients in the comorbid COPD group. PON1 and AE activities were measured spectrophotometrically. RESULTS: Mean age was higher and male gender was more prevalant in COPD than other groups. Fasting blood glucose, LDL-cholesterol, triglyceride, leucocyte counts, erythrocyte sedimentation rate, and hs-CRP levels were higher in COPD patients. Although PON1 and AE were lower in patients than controls, no difference was found between the asthma and COPD groups, nor between pure and comorbid COPD patients. CONCLUSIONS: Although asthma and COPD are two different conditions PON1 and AE activities cannot be markers of differantial diagnosis as they overlap. Comorbid COPD patients may have similar enzyme levels because of the drugs such as statins and aspirin.

Evaluation of apoptosis in cerebrospinal fluid of patients with severe head injury.

OBJECTIVE: To determine whether sFas, caspase-3, proteins which propagate apoptosis, and bcl-2, a protein which inhibits apoptosis, would be increased in cerebrospinal fluid (CSF) in patients with severe traumatic brain injury (TBI) and to examine the correlation of sFas, caspase-3, and bcl-2 with each other and with clinical variables. METHODS: sFas, caspase-3, and bcl-2 were measured in CSF of 14 patients with severe TBI on days 1, 2, 3, 5, 7, and 10 post-trauma. The results were compared with CSF samples from control patients who had no brain and spinal pathology and had undergone spinal anesthesia for some other reason. Soluble Fas and bcl-2 were measured by ELISA while caspase-3 was measured enzymatically. RESULTS: No sFas, caspase-3, and bcl-2 activities were found in CSF of controls, but activities significantly increased in CSF of patients at all time points post-trauma (p < 0.01). Caspase-3 significantly correlated to intracranial pressure (p = 0.01) and cerebral perfusion pressure (p = 0.04). Soluble Fas and caspase-3 peaks coincided on day 5 post-trauma and there was significant association between sFas and caspase-3 increase (p = 0.01). CONCLUSION: This study indicates a prolonged activation of pro-apoptotic (sFas, caspase-3) and anti-apoptotic (bcl-2) proteins after severe TBI in humans. The degree of activation of particularly caspase-3 may be related to the severity of the injury. Parallel increases of these three molecules may indicate a pivotal role of apoptosis in the pathophysiology of post-traumatic brain oedema, secondary cell destruction and chronic cell loss following severe TBI and may open new targets for post-traumatic therapeutic interventions.