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1.
Sci Rep ; 14(1): 20894, 2024 09 08.
Artículo en Inglés | MEDLINE | ID: mdl-39245771

RESUMEN

This study proposes an innovative approach to combat the escalating threat of antibiotic resistance in bacteria by introducing a novel ZnO-propolis nanocomposite (ZnO-P NCs). The overuse of antibiotics, particularly during events like the COVID-19 pandemic, has intensified bacterial resistance, necessitating innovative solutions. The study employs a cost-effective and controllable biosynthesis method to produce ZnO nanoparticles (ZnO-NPs), with propolis extract crucially contributing to the reduction and stabilization of Zn2+ ions. A biodegradable nano-propolis matrix is then created by incorporating ZnO-NPs, forming the ZnO-P NCs. Structural stability is confirmed through FT-IR and Zeta potential analysis, while nanoscale properties are validated via TEM, SEM, and XRD analyses. The antimicrobial efficacy of various substances, including propolis, nano propolis, ethanolic propolis extract, ZnO-NPs, and ZnO-P NCs, is assessed against Gram-negative and Gram-positive bacteria, alongside a comparison with 28 antibiotics. Among the bacteria tested, Pseudomonas aeruginosa PAO1 ATCC15692 was more sensitive (40 mm) to the biosynthesized nanocomposite ZnO-P NCs than to ZnO-NPs (38 mm) and nanopropolis (32 mm), while Escherichia coli was resistant to nanopropolis (0 mm) than to ZnO-NPs (31 mm), and ZnO-P NCs (34 mm). The study reveals a synergy effect when combining propolis with green-synthesized ZnO-NPs in the form of ZnO-P NCs, significantly improving their efficiency against all tested bacteria, including antibiotic-resistant strains like E. coli. The nanocomposite outperforms other materials and antibiotics, demonstrating remarkable antibacterial effectiveness. SEM imaging confirms the disruption of bacterial cell membranes by ZnO-NPs and ZnO-P NCs. The study emphasizes the potential applications of ZnO-NPs integrated into biodegradable materials and underscores the significance of the zinc oxide-propolis nanocomposite in countering antimicrobial resistance. Overall, this research offers a comprehensive solution to combat multidrug-resistant bacteria, opening avenues for novel approaches in infection control.


Asunto(s)
Antibacterianos , Pruebas de Sensibilidad Microbiana , Nanocompuestos , Própolis , Óxido de Zinc , Óxido de Zinc/química , Óxido de Zinc/farmacología , Própolis/química , Própolis/farmacología , Antibacterianos/farmacología , Antibacterianos/química , Nanocompuestos/química , Pseudomonas aeruginosa/efectos de los fármacos , Farmacorresistencia Bacteriana/efectos de los fármacos , Humanos , Espectroscopía Infrarroja por Transformada de Fourier , Nanopartículas del Metal/química
2.
Artículo en Inglés | MEDLINE | ID: mdl-38980482

RESUMEN

Water softening is a treatment process required to remove calcium (Ca(II)) and magnesium (Mg(II)) cations from water streams. Nanocomposites can provide solutions for such multiple challenges and have high performance and low application costs. In this work, a multimetallic cobalt, nickel, and copper 2-aminoterephthalic acid metal-organic framework ((Co/Ni/Cu-NH2BDC) MOF) was synthesized by a simple solvothermal technique. This MOF was supported on an Egyptian natural zeolite ore and was used for the adsorption of Ca(II) ions for water-softening applications. The adsorbent was characterized using Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), N2 adsorption-desorption isotherms, and zeta potential measurements. The adsorption isotherm data for the prepared adsorbent toward Ca(II) were best fit using the Redlich-Peterson model and showed a maximum adsorption capacity of 88.1 mg/g. The adsorption kinetics revealed an equilibrium time of 10 min, which was best fit using the Avrami model. The intermolecular interactions of Ca(II) ions with zeolite and MOF were investigated by Monte Carlo simulations, molecular dynamics simulations, and FTIR and XRD analyses. The adsorption sites in the zeolite structure were oxygen atoms, while those in the MOF structure were amine nitrogen atoms. The Ca(II) ions are coordinated with the solvent molecules in both structures. Finally, the in vitro cytotoxicity of this nanocomposite was assessed, revealing viability levels of 74.57 ± 2.1% and 21 ± 2.79% for Vero and African green monkey kidney and human liver (HepG2) cells, respectively. Cytotoxicity assays help assess the environmental impact of these materials, ensuring that they do not harm aquatic organisms or disrupt ecosystems. Thus, this study demonstrated the valorization of MOF/zeolite as a valuable and industry-ready adsorbent that can appropriate Ca(II) contaminants from aqueous streams.

3.
RSC Adv ; 14(21): 14815-14834, 2024 May 02.
Artículo en Inglés | MEDLINE | ID: mdl-38716105

RESUMEN

Layered double hydroxides (LDH) are promising 2D nanomaterials being investigated for several engineering and biomedical applications. In this work, quinary Zr Al Fe Co Ni LDH and its Al Fe Co Ni LDH quaternary and Fe Co Ni LDH tertiary roots were prepared and characterized. All samples showed an aggregated, layered morphology with zero surface charge and approximately 300 nm of hydrodynamic size. BET surface area of Al Fe Co Ni LDH showed a remarkable value of 143.25 m2 g-1 as opposed to 26.2 m2 g-1 and 45.4 m2 g-1 for Fe Co Ni LDH and Zr Al Fe Co Ni LDH, respectively. The antimicrobial activity of the prepared samples was assessed against the many pathogenic bacteria; Bacillus (B.) subtilis, Escherichia (E.) coli, Haemophilus (H.) influenza, Listeria (L.) monocytogenes, Staphylococcus (S.) aureus, and Streptococcus (St.) pneumonia, and six fungal species. Furthermore, anti-biofilm activity, growth curve assay, and effect of UV illumination were examined against various pathogenic microbes. Zr Al Fe Co Ni displayed remarkable antibacterial activity, as indicated by the lowest values of the minimum inhibitory concentrations (MIC) of 4-166.7 µg mL-1. Results for fungal strains varied in terms of their susceptibilities for the different samples tested. Zn Al Fe Co Ni was able to inhibit the biofilm formation of S. aureus (96.09%), E. coli (98.32%), and Candida (C.) albicans (95.93%). This study shown that certain LDH categories, particularly Zr Al Fe Co Ni, may be promising antibacterial agents against variety of pathogenic microorganisms that cause serious infections.

4.
J Contam Hydrol ; 264: 104364, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38749070

RESUMEN

The increase in antibiotic residues poses a serious threat to ecological and aquatic environments, necessitating the development of cost-effective, convenient, and recyclable adsorbents. In our study, we used cellulose-based layered double hydroxide (LDH) as an efficient adsorbent and nanocarrier for both sulfamethoxazole (SMX) and cefixime (CFX) residues due to their biodegradability and biocompatibility. Chemical processes are measured according to green chemistry metrics to identify which features adhere to the principles. A GREEnness Assessment (ESA), Analytical GREEnness Preparation (AGREEprep), and Analytical Eco-Scale Assessments (ESA) were used to assess the suitability of the proposed analytical method. We extensively analyzed the synthesized CoFe LDH/cellulose before and after the adsorption processes using XRD, FTIR, and SEM. We investigated the factors affecting the adsorption process, such as pH, adsorbent dose, concentrations of SMX and CFX and time. We studied six nonlinear adsorption isotherm models at pH 5 using CoFe LDH, which showed maximum adsorption capacities (qmax) of 272.13 mg/g for SMX and 208.00 mg/g for CFX. Kinetic studies were also conducted. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was performed on Vero cells in direct contact with LDH nanocomposites to evaluate the cytotoxicity and side effects of cellulose-based CoFe LDH. The cellulose-based CoFe LDH nanocomposite demonstrated excellent cytocompatibility and less cytotoxic effects on the tested cell line. These results validate the potential use of these unique LDH-based cellulose cytocompatible biomaterials for water treatment applications. The cost of the prepared adsorbents was investigated.


Asunto(s)
Cefixima , Celulosa , Sulfametoxazol , Contaminantes Químicos del Agua , Celulosa/química , Sulfametoxazol/química , Sulfametoxazol/toxicidad , Adsorción , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidad , Animales , Cefixima/química , Antibacterianos/química , Antibacterianos/toxicidad , Células Vero , Hidróxidos/química , Chlorocebus aethiops , Nanocompuestos/química , Nanocompuestos/toxicidad , Tecnología Química Verde/métodos
5.
Artif Cells Nanomed Biotechnol ; 52(1): 131-144, 2024 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-38423087

RESUMEN

Most fungal bone and joint infections (arthritis) are caused by Mucormycosis (Mucor indicus). These infections may be difficult to treat and may lead to chronic bone disorders and disabilities, thus the use of new antifungal materials in bone disorders is vital, particularly in immunocompromised individuals, such as those who have contracted coronavirus disease 2019 (COVID-19). Herein, we reported for the first time the preparation of nitrogen-doped carbon quantum dots (N/CQDs) and a nitrogen-doped mesoporous carbon (N/MC) using a quick micro-wave preparation and hydrothermal approach. The structure and morphology were analysed using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and surface area analyser. Minimum inhibitory concentration (MIC), disc diffusion tests, minimum fungicidal concentration (MFC) and antifungal inhibitory percentages were measured to investigate the antifungal activity of N/CQDs and N/MC nanostructures. In addition to the in vivo antifungal activity in rats as determined by wound induction and infection, pathogen count and histological studies were also performed. According to in vitro and in vivo testing, both N/CQDs with small size and N/MC with porous structure had a significant antifungal impact on a variety of bone-infecting bacteria, including Mucor infection. In conclusion, the present investigation demonstrates that functional N/CQDs and N/MC are effective antifungal agents against a range of microbial pathogenic bone disorders in immunocompromised individuals, with stronger and superior fungicidal activity for N/CQDs than N/MC in vitro and in vivo studies.


Asunto(s)
Mucormicosis , Puntos Cuánticos , Ratas , Animales , Puntos Cuánticos/química , Antifúngicos/farmacología , Carbono/farmacología , Carbono/química , Nitrógeno/química
6.
Sci Rep ; 14(1): 3990, 2024 02 17.
Artículo en Inglés | MEDLINE | ID: mdl-38368467

RESUMEN

Fluoxetine (FLX) is one of the most persistent pharmaceuticals found in wastewater due to increased use of antidepressant drugs in recent decades. In this study, a nanocomposite of ternary ZnCoAl layered double hydroxide supported on activated carbon (LAC) was used as an adsorbent for FLX in wastewater effluents. The nanocomposite was characterized using Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), and surface area analysis (BET). The adsorption investigations showed that the maximum removal capacity was achieved at pH 10, with a 0.1 g/L adsorbent dose, 50 mL volume of solution, and at a temperature of 25 °C. The FLX adsorption process followed the Langmuir-Freundlich model with a maximum adsorption capacity of 450.92 mg/g at FLX concentration of 50 µg/mL. Density functional theory (DFT) computations were used to study the adsorption mechanism of FLX and its protonated species. The safety and toxicity of the nanocomposite formed from the adsorption of FLX onto LAC (FLX-LAC) was investigated in male albino rats. Acute toxicity was evaluated using probit analysis after 2, 6, and 24 h to determine LD50 and LD100 values in a rat model. The FLX-LAC (20 mg/kg) significantly increased and lengthened the sleep time of the rats, which is important, especially with commonly used antidepressants, compared to the pure standard FLX (7 mg/kg), regular thiopental sodium medicine (30 mg/kg), and LAC alone (9 mg/kg). This study demonstrated the safety and longer sleeping duration in insomniac patients after single-dose therapy with FLX-LAC. Selective serotonin reuptake inhibitors (SSRIs) like FLX were found to have decreased side effects and were considered the first-line mood disorder therapies.


Asunto(s)
Nanocompuestos , Contaminantes Químicos del Agua , Humanos , Masculino , Animales , Ratas , Fluoxetina , Aguas Residuales , Hidróxidos/química , Antidepresivos , Nanocompuestos/química , Adsorción , Cinética , Contaminantes Químicos del Agua/toxicidad , Contaminantes Químicos del Agua/química , Espectroscopía Infrarroja por Transformada de Fourier , Concentración de Iones de Hidrógeno
7.
Artif Cells Nanomed Biotechnol ; 51(1): 590-603, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-37902268

RESUMEN

Nanotechnology holds substantial promise in the innovative therapies for rheumatoid arthritis (RA). The current study was designed to synthesize and characterize a new graphene titanate nanocomposite (GTNc) and explore its anti-arthritic, anti-inflammatory, and antioxidant potencies against Complete Freund's adjuvant (CFA)-induced arthritis in rats, as well as investigate the underlying molecular mechanisms. Our characterization methods included XRD, FT-IR, SEM, EDX, zeta potential, practical size, and XRF to characterize the novel GTNc. Our findings revealed that arthritic rats treated with GTNc exhibited lower levels of RF, CRP, IL-1ß, TNF-α, IL-17, and ADAMTS-5, and higher levels of IL-4 and TIMP-3. In arthritic rats, GTNc reduced LPO levels while increasing GSH content and GST antioxidant activity. Additionally, GTNc decreased the expression of the TGF-ß mRNA gene in arthritic rats. Histopathological investigation showed that GTNc reduced inflammatory cell infiltration, cartilage degradation, and bone destruction in joint injuries caused by CFA in the arthritic rats. Collectively, the anti-arthritic, anti-inflammatory, and antioxidant properties of GTNc appear promising for future arthritis treatments and bone disability research.


Asunto(s)
Artritis Experimental , Grafito , Ratas , Animales , Grafito/farmacología , Antioxidantes/uso terapéutico , Espectroscopía Infrarroja por Transformada de Fourier , Artritis Experimental/inducido químicamente , Artritis Experimental/tratamiento farmacológico , Artritis Experimental/metabolismo , Adyuvante de Freund/efectos adversos , Antiinflamatorios/farmacología
8.
Micromachines (Basel) ; 13(12)2022 Dec 02.
Artículo en Inglés | MEDLINE | ID: mdl-36557433

RESUMEN

Graphitic carbon nitride (G-C3N4) and NiS-NiO/G-C3N4 nanocomposite have been synthesized via combustion and hydrothermal techniques, respectively. The chemical and morphological properties of these materials were confirmed using different analytical methods. SEM confirms the formation of G-C3N4 sheets containing additional petal-like shapes of NiS-NiO nanoparticles. The electrochemical testing of NiS-NiO/G-C3N4 symmetric supercapacitors is carried out from 0.6 M HCl electrolyte. Such testing includes charge/discharge, cyclic voltammetry, impedance, and supercapacitor stability. The charge/discharge time reaches 790 s at 0.3 A/g, while the cyclic voltammetry curve forms under a high surface area. The produced specific capacitance (CS) and energy density values are 766 F/g and 23.55 W.h.kg-1, correspondingly.

9.
Micromachines (Basel) ; 13(12)2022 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-36557533

RESUMEN

Graphitic carbon nitride (G-C3N4) was synthesized through the direct combustion of urea in the air. The CoS-Co2O3/G-C3N4 composite was synthesized via the hydrothermal method of G-C3N4 using cobalt salts. The morphological and chemical structures were determined through XRD, XPS, SEM, and TEM. XRD and XPS analyses confirmed the chemical structure, function groups, and elements percentage of the prepared nanocomposite. SEM measurements illustrated the formation of G-C3N4 sheets, as well as the flower shape of the CoS-Co2O3/G-C3N4 composite, evidenced through the formation of nano appendages over G-C3N4 sheets. TEM confirmed the 2D nanosheets of G-C3N4 with an average width and length of 80 nm and 170 nm, respectively. Two symmetric electrodes for the supercapacitor from the CoS-Co2O3/G-C3N4 composite. Electrochemical measurements were carried out to determine the charge/discharge, cyclic voltammetry, stability, and impedance of the prepared supercapacitor. The measurements were carried out under acid (0.5 M HCL) and basic (6.0 M NaOH) mediums. The charge and discharge lifetime values in the acid and base medium were 85 and 456 s, respectively. The cyclic voltammetry behavior was rectangular in a base medium for the pseudocapacitance feature. The supercapacitor had 100% stability retention up to 600 cycles; then, the stability decreased to 98.5% after 1000 cycles. The supercapacitor displayed a specific capacitance (CS) of 361 and 92 F/g, and an energy density equal to 28.7 and 30.2 W h kg-1 in the basic and acidic mediums, respectively. Our findings demonstrate the capabilities of supercapacitors to become an alternative solution to batteries, owing to their easy and low-cost manufacturing technique.

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