1.
J Bacteriol
; 185(23): 7001-7, 2003 Dec.
Artículo
en Inglés
| MEDLINE
| ID: mdl-14617666
RESUMEN
Most LuxR homologues function as activators of transcription during the process of quorum sensing, but a few, including EsaR and ExpR(Ecc), negatively impact gene expression. The LuxR-activated luxI promoter and LuxR binding site, the lux box, were used in artificial contexts to assess the potential for transcriptional activation and DNA binding by EsaR and ExpR(Ecc). Although the acyl-homoserine lactone responsiveness of both proteins is the opposite of that shown by most LuxR family members, EsaR and ExpR(Ecc) have preserved the ability to interact with RNA polymerase and activate transcription despite their low affinity for the lux box DNA.