[Source and Cause Analysis of High Concentration of Inorganic Aerosol During Two Typical Pollution Processes in Winter over Tianjin].

Inorganic aerosol is the main component of haze days in winter over Tianjin. In this study, two typical high concentrations of secondary inorganic aerosol (SIA) processes, defined as CASE1 and CASE2, were selected during polluted days in January 2020 over Tianjin, and the effects of meteorological factors, regional transport, and chemical processes were comprehensively investigated combined with observations and numerical models (WRF-NAQPMS). The average SIA concentrations in CASE1 and CASE2 were 76.8 µg·m-3 and 66.0 µg·m-3, respectively, and the nitrate concentration was higher than that of sulfate and ammonium, which were typical nitrate-dominated pollution processes. Meteorological conditions played a role in inorganic aerosol formation. The temperature of approximately -6-0℃ and 2-4℃ and the relative humidity of 50%-60% and 80%-100% would be suitable conditions for the high SIA concentration (>80 µg·m-3) in CASE1, whereas the temperature of approximately 2-4℃ and the relative humidity of 60%-70% would be suitable in CASE2. The average contribution rates of external sources to SIA in the CASE1 and CASE2 processes were 62.3% and 22.1%, which were regional transport-dominant processes and local emission-dominant processes, respectively. The contribution of the local emission of CASE1 to nitrate and sulfate was 16.2 µg·m-3 and 8.2 µg·m-3, respectively, higher than that of external sources (31.7 µg·m-3 and 8.8 µg·m-3). the local contribution of CASE2 to nitrate and sulfate was 29.3 µg·m-3 and 25.1 µg·m-3, respectively, whereas the contribution from external sources was 8.1 µg·m-3 and 9.4 µg·m-3, respectively. The quantitative result indicated that local formation and regional transport resulted in higher nitrate concentration than sulfate in CASE1, in contrast to only local sources in CASE2. The gas phase reaction was the main source of inorganic aerosol formation, contributing 48.9% and 57.8% in CASE1 and CASE2, respectively, whereas the heterogeneous reactions were also important processes, with contribution rates of 48.1% and 42.2% to SIA. The effect of aqueous phase reaction was negligible.

The dynamic features and microbial mechanism of nitrogen transformation for hydrothermal aqueous phase as fertilizer in dryland soil.

Hydrothermal aqueous phase (HAP) contains abundant organics and nutrients, which have potential to partially replace chemical fertilizers for enhancing plant growth and soil quality. However, the underlying reasons for low available nitrogen (N) and high N loss in dryland soil remain unclear. A cultivation experiment was conducted using HAP or urea to supply 160 mg N kg-1 in dryland soil. The dynamic changes of soil organic matters (SOMs), pH, N forms, and N cycling genes were investigated. Results showed that SOMs from HAP stimulated urease activity and ureC, which enhanced ammonification in turn. The high-molecular-weight SOMs relatively increased during 5-30 d and then biodegraded during 30-90 d, which SUV254 changed from 0.51 to 1.47 to 0.29 L-1 m-1. This affected ureC that changed from 5.58 to 5.34 to 5.75 lg copies g-1. Relative to urea, addition HAP enhanced ON mineralization by 8.40 times during 30-90 d due to higher ureC. It decreased NO3-N by 65.35%-77.32% but increased AOB and AOA by 0.25 and 0.90 lg copies g-1 at 5 d and 90 d, respectively. It little affected nirK and increased nosZ by 0.41 lg copies g-1 at 90 d. It increased N loss by 4.59 times. The soil pH for HAP was higher than that for urea after 11 d. The comprehensive effects of high SOMs and pH, including ammonification enhancement and nitrification activity inhibition, were the primary causes of high N loss. The core idea for developing high-efficiency HAP fertilizer is to moderately inhibit ammonification and promote nitrification.

The expression of Nrf2 and TLRs in ear effusion in children with different types of otitis media and their relationship with inflammatory factors.

OBJECTIVE: This study aimed to analyze the differences in the expression of Toll-like receptors (TLRs) and nuclear factor erythroid 2-related factor 2 (Nrf2) in ear effusion in children with different types of otitis media (OM), to elaborate the relationship between the expression of TLRs and Nrf2 in ear effusion and the pathogenesis of OM, and to explore the relationship between the two indicators and pro-inflammatory cytokines in children with OM, thereby laying a scientific foundation for revealing the underlying molecular mechanisms of the progression of different types of OM. METHODS: A total of 73 children with OM who were treated in our hospital from March 2019 to July 2021 were selected as the study subjects. By using the cross-sectional investigation method, participants were divided into three groups according to the different pathological types, including the secretory OM group (30 cases), the chronic suppurative OM group (27 cases), and the cystic lesional OM group (16 cases). The levels of Nrf2, TLR2, TLR4 and proinflammatory cytokines [interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), transforming growth factor-ß 1(TGF-ß1), procalcitonin (PCT) and interleukin-1ß (IL-1ß)] were detected in ear effusion of children with different types of OM. Linear regression was used to analyze the correlation between the Nrf2, TLR2 and TLR4 expression levels and pro-inflammatory cytokines in ear effusion. RESULTS: The expression levels of TNF-α and PCT in the ear effusion of the children under 3 years old were significantly higher than that of the children between 3 and 5 years old and that of the children between 6 and 8 years old (all P < 0.001). The mRNA levels of Nrf2, TLR2 and TLR4 in the ear effusion of the children from the chronic suppurative OM group were higher than these from the secretory OM group (P < 0.001, P = 0.008 and P = 0.021). The mRNA levels of Nrf2, TLR2, and TLR4 in the ear effusion of the children from the cystic lesional OM group were higher than those from the chronic suppurative OM group (P < 0.001, P = 0.029 and P = 0.018). A prominent increase in the concentrations of IFN-γ, TNF-α, TGF-ß1, PCT and IL-1ß was found in the ear effusion of children from the chronic suppurative OM group compared to these from the secretory OM group (P = 0.021, P = 0.044, P = 0.048, P = 0.004 and P = 0.001). The concentrations of IFN-γ, TNF-α, TGF-ß1, PCT and IL-1ß in the ear effusion of the children from the cystic lesional OM group were markedly increased as compared with these from the chronic suppurative OM group (P < 0.001, P = 0.004, P = 0.003, P < 0.001 and P < 0.001). Nrf2, TLR2 and TLR4 were taken as independent variables, and inflammatory indexes, including IFN-γ, TNF-α, TGF-ß1, PCT and IL-1ß were used as dependent variables for the linear regression analysis. The results showed that Nrf2, TLR2 and TLR4 were positively correlated with the secretion levels of pro-inflammatory cytokines after adjusting for age, sex, course and the OM classification (all P < 0.05). CONCLUSION: The expressions of Nrf2, TLR2 and TLR4 in the ear effusion of children with different types of OM gradually increased with the severity of the disease, these were significantly positively correlated with the pro-inflammatory cytokines of the children. Nrf2/TLR signaling pathway maintained chronic inflammation in OM, induced damage of middle ear tissue, and promoted the transition from acute OM to chronic OM.

Nrf2 orchestrates transition from acute to chronic otitis media through inflammatory macrophages.

Introduction: Acute and chronic otitis media (AOM and COM) are common middle ear infections that can lead to hearing loss and other complications. Recent research has shown that both macrophages and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway are involved in the immune response to and the resolution of otitis media. However, the specific effects of Nrf2 on macrophages in the transition of AOM to COM are not well understood, and a practical approach to prevent this transition by targeting Nrf2/macrophages has not been established. Methods: In an AOM mouse model using lipopolysaccharide (LPS) injection into the middle ear, middle ear effusion (OME)-macrophages were isolated and analyzed for Nrf2 expression. M2-like polarization of macrophages was induced by Nrf2 activation and its effects on inflammatory resolution were studied by examining inflammatory neutrophils and macrophages, proinflammatory cytokines, and oxidative levels. The survival of human middle ear epithelial cells (HMMECs) co-cultured with Nrf2-modified macrophages was also evaluated. Furthermore, restoration of Nrf2 in macrophages with adeno-associated virus (AAV) vectors was performed to determine the effect on the transition of AOM to COM in experimental mice. Results: Reduced Nrf2 in OME-macrophages during the recovery phase was associated with uncured AOM or its development into COM, demonstrated by persistent increases in inflammatory neutrophils and macrophages, proinflammatory cytokines, and oxidative levels. Nrf2 activation induced M2-like polarization of macrophages, which improved the survival of co-cultured HMMECs treated with LPS in vitro. Restoration of Nrf2 in OME-derived low-Nrf2-expressing macrophages with AAV vectors significantly inhibited the transition of AOM to COM in experimental mice. Discussion: Nrf2 in macrophages plays a critical role in the immune response to and resolution of otitis media Restoration of Nrf2 expression in OME-macrophages could be a promising therapeutic approach to prevent the development of COM in AOM patients.

[Spatio-temporal Distribution Characteristics of Secondary Aerosol in Beijing-Tianjin-Hebei Urban Agglomeration in Winter].

The secondary component is an important factor causing PM2.5 pollution in the Beijing-Tianjin-Hebei urban agglomeration in winter. In this study, the CO tracer method was used to estimate the secondary PM2.5 concentration of the Beijing-Tianjin-Hebei urban agglomeration in the winter of 2017-2021. The temporal and spatial distribution characteristics were analyzed, and the influencing factors of regional secondary PM2.5 were discussed. The results showed that the decreasing trend of PM2.5 concentration in the Beijing-Tianjin-Hebei Region in the winter of 2017-2021 was obvious, and the cities with the largest decline were located in the central and southern part of Hebei Province, mainly contributed by primary PM2.5. There was a good correlation between secondary PM2.5 and PM2.5 in all cities of the Beijing-Tianjin-Hebei urban agglomeration, and the proportion of secondary PM2.5 in Beijing and Tianjin was significantly higher than that in other cities. With the aggravation of pollution degree, the mass concentration of primary PM2.5 and secondary PM2.5 increased in varying degrees, and the proportion of secondary PM2.5 increased significantly. Compared with the direct measurement results, the estimated value obtained by this method was lower as a whole. The selection of appropriate primary aerosol reference value was the key to improving this method and estimating the secondary PM2.5 concentration.

Impacts of meteorology and precursor emission change on O3 variation in Tianjin, China from 2015 to 2021.

Deterioration of surface ozone (O3) pollution in Northern China over the past few years received much attention. For many cities, it is still under debate whether the trend of surface O3 variation is driven by meteorology or the change in precursors emissions. In this work, a time series decomposition method (Seasonal-Trend decomposition procedure based on Loess (STL)) and random forest (RF) algorithm were utilized to quantify the meteorological impacts on the recorded O3 trend and identify the key meteorological factors affecting O3 pollution in Tianjin, the biggest coastal port city in Northern China. After "removing" the meteorological fluctuations from the observed O3 time series, we found that variation of O3 in Tianjin was largely driven by the changes in precursors emissions. The meteorology was unfavorable for O3 pollution in period of 2015-2016, and turned out to be favorable during 2017-2021. Specifically, meteorology contributed 9.3 µg/m3 O3 (13%) in 2019, together with the increase in precursors emissions, making 2019 to be the worst year of O3 pollution since 2015. Since then, the favorable effects of meteorology on O3 pollution tended to be weaker. Temperature was the most important factor affecting O3 level, followed by air humidity in O3 pollution season. In the midday of summer days, O3 pollution frequently exceeded the standard level (>160 µg/m3) at a combined condition with relative humidity in 40%-50% and temperature > 31°C. Both the temperature and the dryness of the atmosphere need to be subtly considered for summer O3 forecasting.

Hsa_Circ_0000826 inhibits the proliferation of colorectal cancer by targeting AUF1.

Many circular RNAs (circRNAs) are reported to be abnormally expressed during the progression of various tumors, and these circRNAs can be used as anti-tumor targets. Therefore, it is important to identify circRNAs that can be used effectively for the clinical diagnosis and treatment of colorectal cancer (CRC). Here, we report that hsa_Circ_0000826 (Circ_0000826), a circRNA with significantly reduced expression level in CRC tissues, is associated with a poor prognosis in patients. The silencing of Circ_0000826 promotes the proliferation of CRC cells. Conversely, the overexpression of Circ_0000826 restricted CRC cell proliferation both in vitro and in vivo. Furthermore, Circ_0000826 could target AU-rich element RNA-binding protein 1 (AUF1). AUF1, known as heterogeneous nuclear ribonucleoprotein D (hnRNP D), could bind to the c-MYC 3'-UTR and promote c-MYC expression. When Circ_0000826 binds to AUF1, it competitively inhibits the binding of AUF1 to the c-MYC 3'-UTR, which inhibits the c-MYC expression and cell proliferation. These results provide novel insights into the functional mechanism of Circ_0000826 action in CRC progression and indicate its potential use as a therapeutic target in CRC.

Hearing characteristics and cochlear implant effects in children with Waardenburg syndrome: a case series.

Background: Waardenburg syndrome (WS) has high clinical and genetic heterogeneity. We aimed to investigate the clinical characteristics of children with WS, and to analyze the effect of cochlear implantation in children with WS who had severe sensorineural hearing loss. Methods: The clinical characteristics of children with WS diagnosed and treated in the past 5 years in the Department of Otolaryngology, Shanghai Children's Hospital were retrospectively analyzed. The 5 WS cases, including 2 males and 3 females, had bilateral severe sensorineural hearing loss. Cochlear implantation was performed between 8 and 21 months old. Audiology tests were conducted, including otoacoustic emissions (OAEs), auditory brainstem response (ABR), and multiple auditory steady-state evoked responses (ASSR). Preoperative computerized tomography (CT) and magnetic resonance imaging (MRI) were performed to evaluate the development of the inner ear and brain. All WS cases were evaluated for hearing and speech abilities before cochlear implantation and at 1 month, 6 months, 12 months, and 24 months after implantation. Results: Among the 5 cases, 3 were WS1, 1 was WS2, and 1 was WS4. All 5 cases received cochlear implantation, and postoperative CT showed that the implant position was good. The infant toddler meaningful auditory integration scale (IT-MAIS) and meaningful use of speech scale (MUSS) scores of all cases increased with hearing age, and IT-MAIS scores were lower than those of normal hearing children of the same age. Conclusions: Children with WS usually have hearing loss. In WS cases with severe sensorineural hearing loss, early cochlear implantation can achieve better hearing and speech development.

Effects of Acute Hypoxic Stress on Physiological and Hepatic Metabolic Responses of Triploid Rainbow Trout (Oncorhynchus mykiss).

This experiment simulated the hypoxic environment caused by actual production operations in fish farming (i.e., catching, gathering, transferring, and weighting) to study the effects of acute hypoxic conditions on the physiological and metabolic responses of triploid rainbow trout (O. mykiss). Two groups of fish weighting 590 g were sampled in the normoxia group (dissolved oxygen above 7 mg/L) and hypoxia group (dissolved oxygen ranged from 2 to 5 mg/L for 10 min). The results showed that 1) regarding stress response, hypoxia increased plasma levels of cortisol, heat shock protein 70 (HSP-70), lysozyme, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatine phosphokinase (CPK); induced the expression of hepatic genes encoding nuclear factor erythroid 2 related factor 2 (Nrf2), interferon γ (IFN-γ) and interleukin-1ß (IL-1ß). 2) Regarding metabolism response, hypoxia increased plasma levels of globulin (GLOB), glucose (GLU), triglyceride (TG) and lactate dehydrogenase (LDH); upregulated the hepatic gene expression of phosphoenolpyruvate carboxykinase, (PEPCK), pyruvate dehydrogenase kinase (PDK1), acetyl-CoA carboxylase (ACC) and acetyl-CoA oxidase (ACO); downregulated the hepatic gene expression of carnitine palmitoyl transferase 1 (CPT1); and unchanged the expression of hepatic genes in glycolysis and autophagy. 3) In response to hypoxia-inducible factors (HIFs), the hepatic HIF-2α gene was activated in the hypoxia group, but HIF-1α gene expression remained unchanged. Thus, during acute hypoxic stress, triploid rainbow trout were in a defensive state, with an enhanced immune response and altered antioxidant status. Additionally, the hepatic mitochondrial oxidation of glucose- and lipid-derived carbon in trout was suppressed, and hepatic gluconeogenesis and lipid synthesis were activated, which might be regulated by the HIF-2α pathway.

Transcriptome Analysis Reveals Crosstalk between the Abscisic Acid and Jasmonic Acid Signaling Pathways in Rice-Mediated Defense against Nilaparvata lugens.

The brown planthopper (BPH) impacts both rice yield and quality. The exogenous application of abscisic acid (ABA) and jasmonic acid (JA) has been previously shown to induce rice resistance to BPH; however, the regulation of rice-mediated defense by these plant growth regulators is unclear. We applied exogenous JA and ABA to rice and analyzed molecular responses to BPH infestation. Nine RNA libraries were sequenced, and 6218 differentially expressed genes (DEGs) were generated and annotated. After ABA + BPH and JA + BPH treatments, 3491 and 2727 DEGs, respectively, were identified when compared with the control (BPH alone). GO enrichment and KEGG pathway analysis showed that the expression of several JA pathway genes (OsAOS2, encoding allene oxide synthase; OsOPR, 12-oxo-phytodienoic acid reductase; and OsACOX, acy1-CoA oxidase) were significantly up-regulated after ABA + BPH treatment. Furthermore, exogenous JA increased the expression of genes involved in ABA synthesis. Meanwhile, the expression levels of genes encoding WRKY transcription factors, myelocytomatosis protein 2 (MYC2) and basic leucine zippers (bZIPs) were up-regulated significantly, indicating that ABA and JA might function together to increase the expression of transcription factors during the rice defense response. The DEGs identified in this study provide vital insights into the synergism between ABA and JA and further contribute to the mechanistic basis of rice resistance to BPH.

[Exploring Formation of Ozone in Typical Cities in Beijing-Tianjin-Hebei Region Using Process Analysis].

As the problem of O3 pollution in the Beijing-Tianjin-Hebei region becomes increasingly prominent, it is of great significance to explore and analyze the ozone variation characteristics and causes of the pollution process in the Beijing-Tianjin-Hebei region for regional air pollution prevention and control. The observations in this study showed that high O3 concentration in spring and summer of the Beijing-Tianjin-Hebei region was higher in the south and lower in the north. The high O3 concentration in Beijing, Tianjin, and Shijiazhuang was often accompanied by the influence of southern wind. Based on WRF-Chem model simulation and process analysis technology, the variation characteristics and causes of O3 in The Beijing-Tianjin-Hebei region in 2019 were deeply analyzed. The diurnal variations in chemical processes, vertical mixing, and transportation in typical cities showed distinct seasonal variations. In summer afternoons, chemical processes were the main source of O3 concentration increase in each city. Vertical mixing resulted in an increase in O3 concentration in Tianjin and Shijiazhuang but a decrease in Beijing. Tianjin and Shijiazhuang had a net output, whereas Beijing had a net inflow. In the polluted O3 process, the chemical process dominated the afternoon O3 concentration increasing in Beijing and Shijiazhuang, whereas vertical mixing dominated in Tianjin. In addition, there was a net input of O3 in Beijing and Shijiazhuang and a net output of O3 in Tianjin. In the clean O3 process, vertical mixing dominated the increase in O3 concentration in Beijing and Shijiazhuang in the afternoon, whereas in Tianjin it was chemical processes. At the same time, the net output of O3 existed in all three cities.

Physiological and Population Responses of Nilaparvata lugens after Feeding on Drought-Stressed Rice.

Drought stress greatly impacts insect development and population growth. Some studies have demonstrated increased reproductive capacity in drought-stressed insects; however, physiological changes in the brown planthopper (BPH), Nilaparvata lugens (Stål), during periods of drought are unclear. In this study, BPH fed on drought- stressed rice had lower population numbers than BPH feeding on non-stressed rice. Water content, osmotic pressure of hemolymph and total amino acid content of BPH were significantly lower when BPH fed on drought-stressed rice compared to the non-stressed control; however, glucose content and glutathione S-transferase (GST) activity were significantly higher in BPH fed on drought-stressed rice. The expression of Vitellogenin and Exuperantia in BPH fed on drought-stressed rice was higher than that in BPH feeding on non-stressed control plants. The size of myofibrils and the abundance of mitochondria in BPH flight muscles were significantly lower in BPH fed on drought-stressed rice compared to non-stressed plants. These results indicate that water management impacts the physiology of BPH, which may be useful in understanding the relationship between drought stress and this damaging herbivore.

[Analysis of Change and Driving Factors of PM2.5 Mass Concentration in Tianjin from 2000 to 2020].

Based on real-time tracking data, PM2.5 mass concentration, and meteorological observations of the Tianjin Meteorological Bureau and the Ecological Environment Bureau, combined with the fine particle meteorological condition diffusion index constructed using the environmental model, the change and driving factors of the PM2.5 mass concentration in Tianjin from 2000 to 2020 were studied to analyze the impact of meteorology on the atmospheric environment. The study showed that change in PM2.5 mass concentration in Tianjin took place in three stages from 2000 to 2020; the first stage showed a continuous increase from 2000 to 2007. The rapid increase in emissions in this stage was the dominant factor, and its effect was four times that of the annual fluctuation in meteorological conditions. The second stage was from 2007 to 2013, in which the PM2.5 mass concentration fluctuated, with two peak years (2007 and 2013). The emissions were stable in this stage. The annual fluctuation of meteorological conditions had an important influence on the annual fluctuation in PM2.5 mass concentration. The third stage was from 2013 to 2020; the PM2.5 mass concentration decreased rapidly, and the decline in emissions was decisive, which reduced the PM2.5 mass concentration by 40% to 50%. The improvement in the meteorological diffusion conditions also provided a positive contribution, which reduced the PM2.5 mass concentration by approximately 10%. Based on the analysis of the data over the past 20 years, the annual variation in atmospheric diffusion conditions caused by the annual variation in meteorological conditions was periodic, with trough values from 2003 to 2004 and 2013 to 2015 and peaks from 2008 to 2010 and 2018 to 2020; the distance between peaks and valleys was approximately 11 years. It was estimated that the next atmospheric diffusion condition valley stage will occur circa 2025. The average intensity of the annual fluctuation in atmospheric diffusion conditions caused by the annual variation in meteorological conditions was 4%, which can explain 25%-50% of the annual variation in PM2.5 mass concentration over the past 20 years, with a difference between peaks and valleys of 16%. The periodic fluctuations in meteorological diffusion conditions have an important impact on the future PM2.5 target setting and corresponding measures design.

Cloning and sequence analysis of a serine protease gene from Rhizoctonia solani Kühn AG5.

The present study aimed to identify the subtilisin-like proteases (SLPs) of Rhizoctonia solani Kühn potentially involved in the virulence of this phytopathogenic fungus, which has 14 anastomosis groups (AGs) responsible for many crop diseases. Through mycelial microscope observation and strain identification of pathogenic fungus MS-3, it was determined to be R. solani AG-5. Both 5' and 3' rapid amplification of cDNA ends were used to clone the serine protease gene RsSLP from R. solani AG-5. The full-length obtained for RsSLP was 1714 bp with an open reading frame of 1587 bp, encoding a protein of 528 amino acids with a molecular mass of 55.8 kDa. This protein contained a predicted signal peptide for secretion but lacked a transmembrane domain or membrane anchor site. Bioinformatics analysis identified this protein as a serine protease with the Peptidase_S8 and Inhibitor_I9 characteristic domains of SLPs. Phylogenetic analysis suggested that frequent gene duplications of the SLPs occurred in R. solani (RsSLP), and RsSLP shares characteristic sequence features with virulence factors of other phytopathogenic fungi. Because the secretory serine protease RsSLP from R. solani AG5 is similar to the virulence factors of other phytopathogenic fungi, its identification will be helpful in studies considering the roles of these proteases in pathogen virulence.

[Cochlear implant and surgical intervention for CHARGE syndrome with laryngeal airway lesions].

Objective:To evaluate the clinical efficacy of surgical intervention for laryngeal airway lesions with concurrent cochlear implantation in CHARGE syndrome concomitant laryngeal airway lesions, and provide clinical data for cochlear implantation in children with CHARGE syndrome concomitant laryngeal airway lesions. Methods:The medical records of five cases diagnosed with CHARGE syndrome were retrospectively reviewed, two of them treated with surgical intervention for laryngeal airway lesions and concurrent cochlear implantation. One child treated with balloon dilatation of laryngeal stenosis and Cochlear implant, and another case received with modified supraglottoplasty for laryngeal malacia and Cochlear implant. Results:Two cases of CHARGE syndrome concomitant laryngeal airway disease, who underwent Cochlear implant and concurrent surgical intervention, recovered well after treatment. The remining three cases treated with Cochlear implant, who previously received deformity-correction surgery. All of the five cases presented with CHD7 mutation. Conclusion:Cochlear implant concurrent with surgical intervention of laryngeal airway lesions for the treatment of CHARGE syndrome concomitant laryngeal airway disease was safe and efficient, which could be a treatment option for children in this situation.

Data Mining-Based Stability and Prescription Analysis of Neonatal Parenteral Nutrition Solution.

In order to evaluate the stability of neonatal parenteral nutrition solution, in this paper, the prescription of neonatal parenteral nutrition solution was investigated and analyzed. The formula of neonatal parenteral nutrition solution used, particularly the one utilized in this study, is commonly used in clinical practice. All the neonatal parenteral nutrition solution required for the test was prepared on the purification workbench in a sterile environment. The time points of stability of parenteral nutrient solution were 0, 12, and 24 hours, respectively, and three parallel samples were taken at each time point. Likewise, to investigate the stability of two kinds of fat milk injection in parenteral nutrition solution of neonates and provide a reference for subsequent experiments and to investigate the influence of electrolyte, amino acid, temperature, pH value, mixing sequence, and the final concentration of glucose on the stability of neonatal parenteral nutrition solution, the stability indexes of neonatal parenteral nutrition liquid mainly include appearance, pH, insoluble particles, fat milk particle size, and particle size distribution. Neonatal parenteral nutrition solution prescriptions from the First Affiliated Hospital of Jinan University, specifically from January to June 2019, were collected and statistically processed. The experimental data were processed by SPSS 19.0 software and data mining technology. The results were expressed as mean ± standard deviation and statistically processed by ANOVA. P < 0.05 was considered statistically significant. The results showed that the stability of neonatal parenteral nutrient solution was influenced by many factors. The formula of neonatal parenteral nutrition solution is generally reasonable, but there are unreasonable phenomena which are needed to be improved further if feasible.

The role of melatonin and Tryptophan-5-hydroxylase-1 in different abiotic stressors in Apis cerana cerana.

Tryptophan-5-hydroxylase-1 (T5H-1) is the rate-limiting enzyme in the biosynthesis of serotonin, which is involved in the biosynthesis of melatonin (Mel). Mel, a biological hormone, plays crucial roles in stressors tolerance, such as cold, hot, Ultraviolet (UV) and pesticide tolerance. However, the direct correlation between T5H-1 and Mel and the underlying mechanism in organisms remains elusive. Mel-mediated cold tolerance was studied extensively in plants and somewhat in insects, including bees. The present study isolated the Mel synthesis gene T5H-1 from Apis cerana cerana for the first time. qRT-PCR analysis indicated that AccT5H-1 played vital roles during some adverse conditions, including 4 °C, 8 °C, 10 °C, 45 °C, UV, cyhalothrin, abamectin, paraquat and bifenthrin exposure. Knockdown of AccT5H-1 using RNA interference (RNAi) technology upregulated most antioxidant genes. Additionally, an enzyme activity assay revealed higher contents of Malondialdehyde (MDA) and Hydrogen peroxide (H2O2), lower content of Vitamin C (VC), and higher activities of Glutathione S-transferase (GST), Superoxide dismutase (SOD), Catalase (CAT) and Peroxidase (POD) in the AccT5H-1 silenced group than the control group. These results suggest that AccT5H-1 is involved in the response to different oxidative stressors in A. cerana cerana. The survival rate of A. cerana cerana exposed to low temperature treatment revealed that the optimal concentration of Mel in the diet was 10 µg/mL. We also found that the antioxidant enzyme (GST, SOD, POD and CAT) concentrations at 10 µg/mL Mel increased to different degrees, and the content of oxidizing substances (MDA and H2O2) decreased, the content of VC increased, and the content of substances that promote cold resistance (glycerol and glycogen) increased. Mel increased the resistance of A. cerana cerana exposed to low temperatures. The expression of AccT5H-1 decreased after the feeding of exogenous Mel to bees. These results provide a reference for other insect studies on Mel and T5H-1.

Maspin inhibits MCF-7 cell invasion and proliferation by downregulating miR-21 and increasing the expression of its target genes.

Maspin has been identified as a tumor suppressor gene in breast cancer, but the underlying regulatory mechanisms remain unclear. In the present study, maspin pcDNA was transfected into MCF-7 cells. microRNA (miR) microarray and reverse transcription-quantitative polymerase chain reaction was used for analysis; the results demonstrated that maspin may inhibit miR-10b, miR-21 and miR-451 expression in MCF-7 cells. In addition, maspin increased the expression of certain miR-21 target genes (phosphatase and tensin homolog, programmed cell death 4 and B-cell lymphoma-2), miR-10b target gene (Homeobox D10; HOXD10) and miR-451 target gene (multidrug resistance protein 1). Furthermore, the results of the present study revealed that decreased expression of miR-21 suppressed the invasion and proliferation of MCF-7 cells. Therefore, in the present study, it was hypothesized that as a tumor-suppressor gene, the potential molecular mechanism of maspin include down-regulating the expression of miR-21 and increasing the expression of specific miR-21 target genes.

Association of long-term sleep habits and hypertension: a cross-sectional study in Chinese adults.

Poor sleep has been associated with risk of hypertension, but previous studies were limited by treating one or two sleep factors as predictor. Our previous study has developed a sleep factor questionnaire (SFQ) to comprehensively assess wide range of sleep characteristics including sleep duration, sleep quality, light at night (LAN) exposure, night/shift work, daytime napping, and frequency of nighttime waking, insomnia, and snoring. In this cross-sectional study we used the SFQ to evaluated the associations between these sleep domains and hypertension. Comparing with the subjects who slept 7-8 h, subjects with sleep duration 6-7 h (OR = 1.42, 95% CI: 1.01, 2.00), 8-9 h (OR = 1.60, 95% CI: 1.07, 2.40), and over 9 h (OR = 2.39, 95% CI: 1.60, 3.58) had an increased prevalence of hypertension. The associations were significant among individuals under 45 years sleeping 6-7 h and those over 45 years sleeping over 8 h. The OR of prevalent hypertension was 0.80 (95% CI: 0.62, 0.97) for habitual daytime napping compared with never napping, and the association was also significant among individuals over 45 years old. Moreover, among subjects with sleep duration 7-8 h, habitual daytime napping may decrease prevalent hypertension (OR = 0.635, 95% CI: 0.437, 0.924). In conclusion, our data suggested a positive association between both short (6-7 h) and long sleep duration and hypertension, and a preventive pattern for habitual daytime napping among over 45 years old and hypertension. Moreover, interaction analysis indicated that habitual midday nap may decrease prevalent hypertension among subjects with sleep duration 7-8 h.

Combination of attenuated Salmonella carrying PD-1 siRNA with nifuroxazide for colon cancer therapy.

Colon cancer is a member of malignant tumors in the digestive system. Traditional treatment strategies are ineffective and improving the treatment of colon cancer is an urgent need. Targeting programmed cell death-1 (PD-1) by monoclonal antibodies has shown some therapeutic effectiveness and has advantages. Additionally, the Stat3 inhibitor nifuroxazide was employed to promote the antitumor activity. Here, we hypothesized that combining nifuroxazide with PD-1 small interfering RNA carried by attenuated Salmonella would exert a synergistic antitumor effect on colon cancer. Indeed, treatment with this combination effectively inhibited the development of colon cancer in mice and improved the survival rate. These two novel anticancer agents worked synergistically to elicit potent antitumor immunity and achieve improved therapeutic efficacy. The underlying mechanisms are mainly involved with immune regulation and cell apoptosis. This study provides a previous framework for combining this Stat3 inhibitor with RNAi designed to block immune checkpoint signaling for cancer therapy.