Expression of LINC01606 in multiple myeloma and its effect on cell invasion and migration.

Given the increasing incidence of multiple myeloma (MM) in recent years, a full understanding of its pathogenesis to find effective molecular markers carries huge implications for future clinical diagnosis and treatment of MM. As the research advances, accumulating studies have pointed out that long non-coding RNAs (LncRNAs) may be the key to the future diagnosis and treatment of neoplastic diseases. OBJECTIVE: This study investigated the clinical implications of LncRNA LINC01606 in MM and its effects on the biological behavior of MM cells. METHODS: In this prospective study, 72 patients with MM (group A) admitted between July 2014 and July 2016 and 68 healthy subjects (group B) who concurrently underwent physical examination in our hospital were included. The expression of LINC01606 in peripheral blood of patients in the two groups was detected to analyze its diagnostic and prognostic value in MM. In addition, MM cells were purchased and transfected with plasmids for mimics, inhibitors and negative control of LINC01606 and miR-579-3p respectively to detect the changes in cell proliferation, invasion and migration. RESULTS: The expression of LINC01606 in group A was higher than that in group B (P<0.050). The sensitivity and specificity of peripheral blood LINC01606 in predicting MM were 85.29% and 72.39%, respectively (P<0.001). Prognostic follow-up analysis revealed higher LINC01606 levels in the dead than those in the survival. The predictive sensitivity of LINC01606 for the 3-year mortality of MM patients was 63.16%, and the specificity was 86.00% (P<0.001). Higher expression of LINC01606 indicated increased risk of 3-year mortality in patients with MM (P<0.001). Compared with LINC01606 overexpression and miR-579-3p inhibition, the proliferation, invasion and migration of cells decreased more significantly by LINC01606 inhibition and miR-579-3p overexpression (P<0.050). Dual luciferase reporter (DLR) assay confirmed the targeting relationship between LINC01606 and miR-579-3p. There was no significant difference in the activity of MM cells co-transfected with LINC01606-inhibitor and miR-579-3p-inhibitor plasmids compared with the blank group (P>0.050). CONCLUSIONS: LINC01606, with a high expression profile in MM, promotes the proliferation, migration and invasion of MM cells through targeted inhibition of miR-579-3p, which may be the key to future diagnosis and treatment of MM.

Comparison of ultrasound-guided puncture drainage and incision drainage for deep neck abscess.

BACKGROUND: Deep neck space abscess is a common disease in otolaryngology-head, and neck surgery emergencies that result in significant morbidity with potential mortality. Traditional incision drainage with antibiotics is widely accepted worldwide. Recent studies have shown that ultrasound-guided drainage is an effective strategy and is less invasive for patients. The present study aimed to explore the difference between puncture and drainage guided by B-ultrasound and traditional surgical incision in treating deep neck space abscess. METHODS: A total of 60 patients with deep neck abscess were enrolled in the present study; 43 were distributed to the B-ultrasound puncture drainage group and 17 to the incision drainage group. Clinical data were collected, and differences between the 2 treatment options were compared. RESULTS: There were no differences in patients' systemic illness, age, and clinical features (diameter of an abscess, amount of drainage in first 3 days, and body temperature). The cure rate of both groups was 100%; the number of hospitalization days of the B-ultrasound-guided puncture group (8 days) was significantly less than that of the incision drainage group (10.8 days). CONCLUSIONS: Puncture drainage of neck abscess guided by B-ultrasound is a safe and effective treatment method and can reduce the patient's hospital stay.

Low CD1c + myeloid dendritic cell counts correlated with a high risk of rapid disease progression during early HIV-1 infection.

BACKGROUND: During early HIV-1 infection (EHI), the interaction between the immune response and the virus determines disease progression. Although CD1c + myeloid dendritic cells (mDCs) can trigger the immune response, the relationship between CD1c + mDC alteration and disease progression has not yet been defined. METHODS: EHI changes in CD1c + mDC counts, surface marker (CD40, CD86, CD83) expression, and IL-12 secretion were assessed by flow cytometry in 29 patients. RESULTS: When compared with the normal controls, patients with EHI displayed significantly lower CD1c + mDC counts and IL-12 secretion and increased surface markers. CD1c + mDC counts were positively correlated with CD4+ T cell counts and inversely associated with viral loads. IL-12 secretion was only positively associated with CD4+ T cell counts. Rapid progressors had lower counts, CD86 expression, and IL-12 secretion of CD1c + mDCs comparing with typical progressors. Kaplan-Meier analysis and Cox regression models suggested patients with low CD1c + mDC counts (<10 cells/µL) had a 4-fold higher risk of rapid disease progression than those with high CD1c + mDC counts. However, no relationship was found between surface markers or IL-12 secretion and disease progression. CONCLUSIONS: During EHI, patients with low CD1c + mDC counts were more likely to experience rapid disease progression than those with high CD1c + mDC counts.

Rapid disease progression in HIV-1-infected men who have sex with men is negatively correlated with peripheral plasmacytoid dendritic cell counts at the early stage of primary infection.

To explore the relationship between absolute dendritic cell (DC) counts at the early stage of primary human immunodeficiency virus type 1 (HIV-1) infection (PHI) and subsequent disease progression, we performed a prospective study of 16 rapid progressors (RPs) and 12 typical progressors (TPs) from a PHI cohort of men who have sex with men. Significantly decreased plasmacytoid DC (pDC) and myeloid DC (mDC) counts in the blood of RPs were observed at study entry as compared with TPs and healthy HIV-1-negative subjects. Low baseline pDC counts were significantly associated with rapid disease progression after adjustment for baseline CD4(+) T cell counts, mDC counts, and HIV-1 load. Kaplan-Meier survival analysis showed that low pDC counts were strongly associated with rapid disease progression. Our findings demonstrated the predictive value of blood absolute pDC counts at baseline in PHI for HIV-1 disease progression. Further studies are required to confirm this notion.

[The long-term morphology of the nasal cavity after total laryngectomy].

OBJECTIVE: To investigate the long-term effect of nasal airflow deprivation on nasal dimensions after total laryngectomy. METHOD: Thirty-two patients with total laryngectomy were enrolled in the study. Acoustic rhinometry was used to measure the minimum cross-sectional area (MCSA) and the volume of the nasal cavity after laryngectomy, compared with the normal control group. In addition, patients underwent endoscopic nasal examinations and answered questionnaires postoperatively, symptoms between the different levels to compare the results of acoustic rhinometry. RESULT: At both within 1-year and more than 1-year follow-ups, the mean MCSAs and the mean nasal volumes were significantly expanded than the control values (P < 0.05). The nasal MCSA, nasal cavity volume was no significant difference between more than one year and less than 1 year group (P > 0.05). The endoscopic examinations revealed only a deterioration in the appearance of the nasal mucosa over the long term. Survey showed that the postoperative patients had varying degrees of nasal obstruction flu, nose dry, hyposmia, etc. The nasal MCSA, nasal cavity volume was of the largest when moderate nasal obstruction flu,or severe dry nose or severe hyposmia. CONCLUSION: The structure was atrophic nasal change, the nasal MCSA, nasal cavity volume were larger, the results of acoustic rhinometry was different among the varying degrees of symptoms after total laryngectomy.

[Objective evaluation of nasal ventilation function in healthy adults in Tianjin area].

OBJECTIVE: To establish reference values of acoustic rhinometry, rhinomanometry and rhinospirometer in healthy adults in Tianjin area, analyze the effects of age,sex and side on the value, investigate the correlation of the measure values, offer the diagnosis date for test nasal ventilation function in Tianjin area. METHOD: Four hundred and sixty-six healthy adults in Tianjin area were tested. A1 acoustic rhinometry was used to measure the minimum cross-sectional area (MCA), distance of the minimal cross-sectional area to the nostril (DCAN) and the nasal volume from 0-5 cm, 2-5 cm (V5, V2-5); At 150 Pa, 75 Pa and broms, NR6 Rhinomanometry was used to measure unilateral nasal inspiratory resistance (IR)and expiratory resistance (ER), bilateral nasal inspiratory and expiratory resistance (TIR and TER), and differences of the bilateral nasal resistance can be calculated; NV1 Rhinospirometer was used to measure unilateral inspiratory capacity (IC) and expiration capacity (EC), and the nasal partitioning ratio (NPR) can be calculated. Practical measure the distance of nostril to ahead of the inferior turbinate and compare with DCAN. Make the correlational analysis on different index of three exam. RESULT: Reference values of acoustic rhinometry: MCA was (0.45 +/- 0. 16) cm2 for male, (0.44 +/- 0.16) cm2 for female; V2-5 was (3.52 +/- 1.38) cm3 for male, (3.36 +/- 1.22) cm3 for female, V5 was (5.10 +/- 1.47) cm3 for male, (4.86 +/- 1.12) cm3 for female; DCAN have two distance, (2.22 +/- 0.398, 0.53 +/- 0.625) cm was for male, (2.10 +/- 0.37, 0.67 +/- 0.15) cm was for female. No significant gender, side and age differences were shown in MCA, V5, V2-5. Significant gender differences were shown in DCAN but no side and age differences. Reference values of rhinomanometry: Significant gender but no side and age differences were shown in IR, ER, TIR, TER. Reference values of rhinospirometer: IC was (2.06 +/- 1.10) L/20 s for male, (1.37 +/- 0.34) L/20 s for female, EC was (2.15 +/- 1.23) L/20 s for male (1.39 +/- 0.58) L/20 s for female. NPRi was 0.11 [0.05, 0.23],NPRe was 0.11 [0.05, 0.19]. Significant gender but no side and age differences were shown in IC and EC. No gender and age differences were shown in NPRi and NPRe. There was significant correlation found between MCA and IR/ER/IC/EC, IR and IC, ER and EC, Rlr and NPRi/ NPRe. CONCLUSION: Acoustic rhinometry,rhinomanometry and rhinospirometer can be useful reference values to evaluate nasal ventilation function, more value will be found if use the three together.

[The analysis of smooth pursuit test in unilateral vestibular peripheral vertigo].

OBJECTIVE: To analyze the result of smooth pursuit test (SPT) in unilateral vestibular peripheral vertigo and investigate its influencing factors. METHOD: Smooth pursuit test (SPT) and spontaneous nystagmus (SN) were examined in one hundred and eighty-five patients with unilateral peripheral vertigo (case group) and 51 normal persons (control group) by Video-Nystagmography (Synapsis, France), and the gain of SPT and SN were selected as the observation parameters in order to analyze the waveform and gain of SPT and the relativity between SN and the gain of SPT. RESULT: Of the 185 patients, 105 (56.8%), 72 (38.9%) and 8 (4.3%) cases produced I , II and III waveforms respectively. Of these patients, 58 (31.4%) demonstrated SN and none had IV waveform. While of 51 normal persons, 38 (74.5%), 13 (25.5%) persons produced I and II waveforms respectively and there were no III, IV waveforms or SN. There was statistical significance between the strong and weak gain of SPT in these two groups. Weak gain was significantly different between two groups. The strong and weak gain of SPT in case group were 0.86 +/- 0.06, 0.80 +/- 0.06; 0.78 +/- 0.09, 0.65 +/- 0.1; 0.68 +/- 0.13, 0.45 +/- 0.12. The relativity between SN and the gain of SPT was positive when they had same direction (r(s) = -0.63, P<0.05) and negative when opposite (r(s) = 0.34, P<0.05). CONCLUSION: I , II, III three waveforms of SPT could appear in unilateral vestibular peripheral vertigo and the corresponding gains are gradually decreasing. SN is the influencing factor of SPT.

[Clinical applications of magnifying laryngoscopy on nasopharyngeal examination.].

OBJECTIVE: To evaluate the usability of magnifying laryngoscopy for the diagnosis of nasopharyngeal diseases. METHODS: An analysis was made on the nasopharyngeal figure and letter data among 6774 cases undergone magnifying laryngoscopy. Among these cases, biopsy would be given to those with suspected lesion in the nasopharynx carried out under magnifying laryngoscopy or endoscope. RESULTS: Compliance of patients to magnifying laryngoscopy at the rate of 98.9% (6700/6774) was superior to indirect nasopharyngoscopy 30.9% (2095/6774). The difference was statistically significant (chi(2) = 6872.75, P < 0.005). Magnifying laryngoscopy could clearly show a variety of diseases in nasopharynx, early detection of suspicious lesions. Among 615 cases, both fiber nasopharyngoscopy (192 cases) and nasal endoscopy (423 cases) with nasopharyngeal uplift were used, magnifying laryngoscopy found 418 cases bilateral symmetry, the surface smooth, no abnormal. The pathological examination confirmed normal tissue. Other 197 cases in the larger laryngoscopy also showed the uplift in nasopharynx by pathologically examination confirmed a variety of benign and maligant lesions. CONCLUSIONS: Magnifying laryngoscopy with strong brightness, high resolution, can early detect the nasopharyngeal lesions, increase the diagnostic accuracy rate and biopsy-positive rate. Therefore, it is valuable for the diagnosis of nasopharyngeal diseases.