Airborne antibiotic and metal resistance genes - A neglected potential risk at e-waste recycling facilities.

Heavy metal-rich environments can promote the selection of metal-resistance genes (MRGs) in bacteria, often leading to the simultaneous selection of antibiotic-resistance genes (ARGs) through a process known as co-selection. To comprehensively evaluate the biological pollutants at electronic-waste (e-waste) recycling facilities, air, soil, and river samples were collected at four distinct Swiss e-waste recycling facilities and analyzed for ARGs, MRGs, mobile genetic elements (MGEs), endotoxins, and bacterial species, with correlations drawn to heavy metal occurrence. To our knowledge, the present work marks the first attempt to quantify these bio-pollutants in the air of e-waste recycling facilities, that might pose a significant health risk to workers. Although ARG and MRG's profiles varied among the different sample types, intl1 consistently exhibited high relative abundance rates, identifying it as the predominant MGE across all sample types and facilities. These findings underscore its pivol role in driving diverse bacterial adaptations to extreme heavy metal exposure by selection and dissemination of ARGs and MRGs. All air samples exhibited consistent profiles of ARGs and MRGs, with blaTEM emerging as the predominant ARG, alongside pbrT and nccA as the most prevalent MRGs. However, one facility, engaged in batteries recycling and characterized by exceptionally high concentrations of heavy metals, showcased a more diverse resistance gene profile, suggesting that bacteria in this environment required more complex resistance mechanisms to cope with extreme metal exposure. Furthermore, this study unveiled a strong association between gram-negative bacteria and ARGs and less with MRGs. Overall, this research emphasizes the critical importance of studying biological pollutants in the air of e-waste recycling facilities to inform robust safety measures and mitigate the risk of resistance gene dissemination among workers. These findings establish a solid foundation for further investigations into the complex interplay among heavy metal exposure, bacterial adaptation, and resistance patterns in such distinctive ecosystems.

[Aspects of animal health, animal welfare and biosecurity during 101 transports of piglets in Switzerland]. / Untersuchung von Tiergesundheit, Tierwohl und Biosicherheit während 101 Transporten von Mastferkeln in der Schweiz.

INTRODUCTION: The transfer of piglets is associated with stress for the transported animals. In addition, animal transports are a risk factor for the spread of pathogenic and antibiotic-resistant bacteria and for the introduction of diseases into the herd. In the present study, 101 randomly selected transports of piglets were accompanied from the breeding facility to the pig farm. Parameters related to animal health, animal welfare and biosecurity were assessed. Transported piglets showed signs of abscesses, hernias or cannibalism in 30% and diarrhea or cough in 15% of the cases. The proportion of animals with injuries from conflicts were higher after (15%) than before transport (8%). Suboptimal conditions in regard to density, temperature and lighting were found in 19%, 55% and 36% of the transports. Vehicles were cleaned and disinfected only in 55% of transports before arriving at the breeding facility. Thirty percent of the vehicles were soiled and 20% had already loaded piglets when arriving at the sending facility. Vehicles were neither cleaned nor disinfected in 83% between two piglet transports. Overall, there was a great potential for improvement in animal loading and risk of disease transmission in the investigated piglet transports.

INTRODUCTION: Les transports sont associés à du stress pour les animaux. Ils sont en outre un facteur de risque pour la dissémination de germes pathogènes et antibio-résistants ainsi que pour l'introduction de maladies dans les effectifs d'animaux. Pour la présente étude, on a accompagné 101 transports de porcelets choisis au hasard depuis l'exploitation de production jusqu'à celle d'engraissement. A cette occasion, des paramètres relatifs à la santé des animaux, à leur bien-être et à la biosécurité ont été jugés. Dans environ 30% des cas, des porcelets présentant des abcès, des hernies ou des signes de cannibalisme ont été transportés et dans 15% des cas des animaux souffrant de diarrhée ou de toux. La proportion d'animaux présentant des traces de bagarres était plus élevée de 15% après le transport qu'avant (8%). En ce qui concerne le taux d'occupation, la température et l'éclairage, des carences ont été constatées dans respectivement 19%, 55% et 36% des transports. Les véhicules n'avaient été nettoyés et désinfectés avant leur arrivée dans l'exploitation de production que dans 55% des cas. Trente pour cent des véhicules étaient souillés par des excréments et 20% transportaient déjà des porcelets à leur arrivée sur l'exploitation. Lors de 83% des transports, les véhicules n'ont été ni nettoyés ni désinfectés entre deux transports de porcelets. Il existe un grand potentiel d'amélioration dans les transports de porcelets que nous avons accompagnés, que ce soit du point de vue de l'exposition des animaux au stress ou de la dissémination potentielle de germes.

"Water-in-salt" electrolytes enable the use of cost-effective aluminum current collectors for aqueous high-voltage batteries.

The extended electrochemical stability window offered by highly concentrated electrolytes allows the operation of aqueous batteries at voltages significantly above the thermodynamic stability limit of water, at which the stability of the current collector potentially limits the cell voltage. Here we report the observation of suppressed anodic dissolution of aluminum in "water-in-salt" electrolytes enabling roll-to-roll electrode fabrication for high-voltage aqueous lithium-ion batteries on cost-effective light-weight aluminum current collectors using established lithium-ion battery technology.

Quantitative analysis of heavy metals in automotive brake linings: a comparison between wet-chemistry based analysis and in-situ screening with a handheld X-ray fluorescence spectrometer.

Two extraction procedures for ecologically relevant elements present in automotive brake linings (Sb, Bi, Pb, Cd, Cr (total), Co, Cu, Mo, Ni, Sr, V, Zn, Sn) were developed and validated, applying a high pressure asher (HPA-S) and microwave extraction, respectively. Both of these methods allowed for the quantitative analysis of the extracted elements by inductively coupled plasma optical emission spectrometry (ICP-OES). The results were compared to measurements using a handheld energy-dispersive X-ray fluorescence spectrometer (ED-XRF), being in discussion by regulating agencies as in-situ screening tool for brake pads. The comparison indicates that the handheld ED-XRF analysis is basically an efficient screening tool for a reliable assessment of trace metal contents in automotive brake pads with respect to legal standards. While a quantitative determination of elements like Cd, Co, Cr, Mn, Mo, Ni, Pb and Sb was achievable, other elements (V, Cu, Bi, Zn, Sn and Sr) could only be determined qualitatively due to the special matrix characteristics of brake pads.