Determination of the Effects of Ankaferd Wound Dressing on the Wound Healing Process in Rats.

Objectives: The effects of a composite nanofiber wound dressing material consisting of a polyvinylidene alcohol and polyvinylidene pyrrolidone polymer mixture with a hemostatic agent doped with Ankaferd Blood Stopper (ABS) on the healing of experimentally induced dermal wounds in rats were examined. Materials and Methods: Rats were divided into 4 groups (n= 6). Histological material was examined on tissues taken from the wound site, whereas total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) analyses were performed on blood samples taken from the cardia. The material that was produced had hydrophilic properties, and both the ABS-doped and-undoped forms of the material positively affected wound healing. Results: In the histopathological examinations, macroscopic evaluations revealed a statistically significant difference between the groups in terms of wound diameter, reepithelialization, and inflammation formation (p= 0.019). In parallel with wound healing and histological outcomes, TAS values increased in the ABS-doped groups, and TOS and OSI values decreased in the wound dressing groups (p < 0.05). Conclusion: It was concluded that the ABS-dopped dressing did not have a negative effect on wound healing, it accelerated healing, and it could be used effectively and safely to treat skin injuries. However, further studies are needed to evaluate the clinical and histopathological benefits and potential adverse effects of wound dressings produced using ABS-doped polymers on wound healing.

Determination of the effect of sunset yellow on the morphological parameters of male mice during the development period.

Sunset Yellow (SY), an azo synthetic food dye, is widely used in the food industry. Although there are different opinions on its effect on people, its use is regulated in the European Union. If the Acceptable Daily Intake of 2.5 mg/kg/bw is exceeded, it may have pathological and biochemical effects on organs. There are not enough studies on the effects of SY on growth and development in mammals. This study was conducted to determine the effect of SY on the morphological parameters of mice at different ages (four, eight, and ten weeks old). The treatment and control groups were created with Swiss Albino mice (n: 6). SY was administered orally for 28 days (30 mg/kg/bw/week). On the last day of the study, the mice were weighed, and tail, temporal region, femur, and crown-rubmp-length values were measured using a digital caliper. A statistical difference in average body weight was observed in the SY groups (p < 0.05). SY administration during childhood caused retardation in growth and development parameters. Therefore, SY may cause weight gain and affect morphological parameters. Additional studies are required to investigate the effects of SY at different doses and durations.

Is Turkish coffee protects Drosophila melanogaster on cadmium acetate toxicity by promoting antioxidant enzymes?

With their increasing use in today's industry, heavy metals cause biochemical and biophysical changes by affecting the control and regulatory systems of living things. Cadmium (Cd), a heavy metal, spreads to the environment through both natural sources and industrial activities. It is taken into the organism through water, food, skin contact or smoke. Systems and organs of living things are directly or indirectly affected by Cd toxicity. Besides their recreational usage, herbal products such as coffee are preferred in alternative medicine because of their antioxidant, anti-inflammatory, anticancer and antidiabetic effects. Turkish coffee (TK) is a drink rich in flavorings, phenolic compounds and antioxidant compounds. The study evaluated the possible antioxidant role of TK against oxidative stress induced by Cadmium acetate (CdA) in the fat tissues of old-young female individuals of Drosophila melanogaster. The female flies were fed with either a standard diet, or CdA (10-30 mg), or TK (2%), or both (CdA + TK) for 3 and 10 days. Following the completion of the feeding period, the amounts of fatbody and oxidative stress markers (oxidative stress index, malondialdehyde), activities of antioxidant enzymes (Glutathione-S-transferase, Catalase, and Superoxide dismutase) and their levels were measured. Fat body lipid droplets were high in the individuals exposed to high concentrations of CdA. It was determined that lipid droplets decreased but did not significantly alter oxidative stress in the individuals treated with TK (p = 0.05). This article may be of help in terms of the use of TK compounds as antioxidants to evaluate their effects in preventing heavy metal accumulation and stress in the aging process.

Investigation of the protective effect of acetazolamide and SLC-0111 on carbon tetrachloride-induced toxicity in fruit fly.

Sulfonamide-based compounds in the development of drugs used in cancer treatment have been started to be investigated recently. In the current work, it was determined the protective effect of Acetazolamide (AAZ) and SLC-0111 on carbon tetrachloride-induced toxicity in the fruit fly (Drosophila melanogaster). AAZ and SLC-0111 were used as a nonselective and selective inhibitor of carbonic anhydrase isozymes, respectively, to compare the selectivity effect of drugs on toxicity. The experimental toxicity was created by carbon tetrachloride (CCl4) that causes tissue damage to the first stage larvae of fruit fly and used as a model organism. The effect of AAZ and SLC-0111 on toxicity of insect survival, sex ratio, longevity and some biochemical parameters such as Malondialdehyde-MDA content, Superoxide dismutase-SOD and Glutathione-S-transferase-GST activity were tested. According to the data obtained, feeding of insects with AAZ and SLC-0111 (2.5 and 10 mM, respectively) affected their survival and development positively against the toxicity induced by CCl4. Compared to the control group, GST and SOD activity was higher in pups and adults (SLC-0111 < AAZ). Because of this study, SLC-0111 is thought to be useful in protecting against the harmful effects of reactive oxygen species.

Evaluation of the hybrid system combining electrocoagulation, nanofiltration and reverse osmosis for biologically treated textile effluent: Treatment efficiency and membrane fouling.

The efficiency of the hybrid electrocoagulation-nanofiltration-reverse osmosis (EC-NF-RO) system for the treatment of biologically treated textile effluent was investigated. The treatment performances and membrane fouling behaviours of nanofiltration (NF) and hybrid EC-NF systems were compared. EC process was evaluated concerning mitigate the membrane fouling and increasing the removal efficiencies. Besides, the treated wastewater with the hybrid EC-NF process was finally processed using RO process for reuse purpose in the textile industry. The EC treatment was applied using Fe and Al electrodes at various conditions; pH:4-10, current density:0.5-17 mA/cm2 and operating time:30-180 min. Fe electrode showed better performance in terms of higher removal efficiencies (76% COD, 96% DFZ436), lower energy (21.1 kWh/m3) and electrode consumptions (3.7 kg/m3) for the optimum conditions. Scanning Electron Microscopy-Energy Dispersive Index (ESEM-EDX) and Fourier-Transform Infrared Spectroscopy (FTIR) analyses were carried out for EC sludge samples obtained with Fe and Al electrodes. Desal 5 DL and NF 270 membranes were tested in terms of removal efficiency and membrane fouling for NF and hybrid EC-NF process of textile wastewater. Membrane fouling was evaluated with flux values, resistance-in-series model results as well as Atomic Force Microscopy (AFM), FTIR and contact angle measurements. NF 270 membrane achieved better chloride (28%) and conductivity (41%) removal efficiencies for NF treatment. EC pretreatment did not result in any noticeable improvement in rejections except for chloride (48%) and conductivity (59%) for the hybrid EC-NF process with NF 270. The ratios of Rc decreased to 40% for NF 270 and 42% for Desal 5DL after EC pretreatment. NF270 membrane indicated high permeate flux and low membrane fouling considering cake resistance distribution, surface roughness, hydrophilicity and chemical structure variation. >93% COD, 99% conductivity, 97% chloride, and 91% TDS removal efficiencies were obtained with the hybrid EC-NF-RO process. Finally, the obtained high quality water by RO after the EC + NF 270 hybrid process could be used for all textile finishing process.

Antioxidant effects of ankaferd blood stopper doped polyvinyl pyrolidon in an experimental model created in insect.

This research evaluated Ankaferd Blood Stopper (ABS)-doped Polyvinylpyrrolidone (PVP) nanofiber layers which were produced with the electrospinning method for their potential for co-use in response to oxidative stress. As a result of the use of such a preparation (ABS doped PVP) in long-term treatments, the response to oxidative stress was compared to biochemical parameters, and its effect on sex was also aimed to be determined. For this purpose, Drosophila melanogaster foods were coated with 10% PVP, ABS (2 ml) and PVP-ABS. In total, 300 flies were randomized into 6 groups, each consisting of 25 female and 25 male insects, and the insects were fed with the determined coated mediums. The effects of foods on adult flies were tested for biochemical changes (Malondialdehyde-MDA and Total oxidation status-TOS, Glutathione-S-Transferase-GST, Catalase-CAT and Superoxide dismutase-SOD activities, Total antioxidant capacity-TAS) at the end of ten days. It was determined that the separate use of the two substances increased the amount of MDA in both sexes. It was found that the combined use of PVP-ABS had a positive effect similar to the control by increasing the antioxidant enzymes (SOD, CAT, GST). Feeding with ABS-doped PVP in the male insects reduced TOS (2.00 ± 0.01 µmol H2O2Eq/L), but the female insects were found to have higher OSI (40.00 ± 0.01 µmol H2O2Eq/L). As a result, PVP-ABS may be used together as an antioxidant, but more detailed studies are needed for their safe use on both sexes.

[Investigation of the Relationship between Empathy and Burnout Levels of Nursing Students]. / Hemsirelik Ögrencilerinin Empati ve Tükenmislik Düzeyleri Arasindaki Iliskinin Incelenmesi.

AIM: This research was designed to determine the relationship between empathy and burnout levels of nursing students. METHOD: The population of this descriptive and cross-sectional research consisted of 1294 students studying at a Nursing Faculty in the 2016-2017 academic year and the sampling consisted of 290 nursing students who were selected by the stratified random sampling method. Data were collected using the "Structured Question Form", the "Empathic Skill Scale B-Form" and the "Maslach Burnout Inventory-Student Form". Written permission and ethical approval were obtained from the faculty where the research would be conducted. Data analysis was performed using the descriptive and significance analysis in the SPSS 21.00 program. RESULTS: 84.1% of the students were female, and the average age was 20.28±1.47. It was determined that the mean score of the Empathic Skill Scale B-Form was 143.86±23.93, and that the mean scores of the sub-dimensions of the Maslach Burnout Inventory-Student Form were 15.11±4,69 in burnout, 9.34±3.62 in depersonalization, and 12.78±2.67 in competency. There is a statistically significant relationship between the Empathic Skill Scale score of nursing students and the Depersonalization sub-dimension score of the Maslach Burnout Inventory (p<0.05). CONCLUSION: It was determined that the burnout and empathy skills of nursing students were at a moderate level and that some socio-demographic characteristics affected their empathy and burnout levels.