In vitro and in vivo assessment of antimicrobial, enzymatic, and antifouling properties of self-potent lichen symbiotic bacteria.

This study aimed to comprehensively assess the antimicrobial, antifouling, and antibiofilm-forming potential of lichen symbiotic bacteria against marine fouling bacterial strains. A total of 50 lichen-associated bacteria (LAB) isolates were successfully characterized and evaluated for their effectiveness in mitigating biofouling caused by various marine biofoulers. Through a battery of biological assays encompassing enzymatic, antagonistic, antimicrobial, and antifouling assays, 15 LAB isolates were identified based on their antagonist activities. Notably, the strain LAB4 exhibited remarkable performance across all bioassays, demonstrating its proficiency as an antifouling agent. The production of crude LAB extracts was successfully scaled up using a large-scale fermentor and further optimized. Additionally, a phylogenetic analysis of the isolated strain Bacillus proteolyticus D65's 16S ribosomal RNA gene revealed a high query coverage and percentage identity of 92.62% (accession no. MK883171.1). In conclusion, the lichen bacterial symbiotic isolate B. proteolyticus exhibited significant in vitro and in vivo inhibition of foulants. This study highlights the potential of lichens as a valuable source of yet unexplored bacteria. The bacterial consortium associated with Parmotrema sp. holds promise in combatting biofouling, which poses a substantial threat to the maritime industries and their economic stability.

Scientific Investigation of Antifouling Activity from Biological Agents and Distribution of Marine Foulers-Coastal Areas of Tamil Nadu.

Biofouling is the result of a biological process that is the accumulation of micro- and macro-organisms on the surfaces of the ship which causes serious environmental problems. The consequence of biofouling includes modifying the hydrodynamic response, affecting heat exchange, can make structures heavier, accelerate or generating corrosion, biodegradation, increasing the fatigue of certain materials, and blocking mechanical functions. It causes severe problems for the objects in the water such as ships and buoys. Also, its impact on shellfish and other aquaculture was sometimes devastating. The main scope of this study is to review the currently available biocides from biological agents for marine submerged foulants and marine foulers that are present around the coastal areas of Tamil Nadu. Biological anti-fouling methods are preferred than that of the chemical and physical anti-fouling methods as it have some toxic effects on the non targeted marine biodiversity. This study focuses on the marine foulers that are present around the coastal areas of Tamil Nadu which will be helpful for the researchers to discover the suitable anti-foulers from a biological source, which will be very useful to protect the marine ecosystem and marine economy. A total of 182 antifouling compounds from marine biological sources were discovered. The marine microbes, Penicillium sp. and Pseudoalteromonas issachenkonii, were reported to possess EC50. The survey results obtained from this study show that Chennai coastal region has a lot of barnacles, and 8 different species were present in Pondicherry region.

Formulation and validation of probioticated foxtail millet laddu as a source of antioxidant for biological system using response surface methodology.

Probiotics play a critical role in supporting a healthy gut microbiome, which significantly impacts overall health and well-being. While there has been an increase in the availability of probiotic foods in recent years, there may still be limited options and accessibility in certain regions. This study focused on formulating a traditional Indian sweet called laddu enriched with millet and Lactobacillus acidophilus. The formulation of laddu ingredients was optimized using Design Expert software to create an optimal product for testing. The probiotic Lactobacillus acidophilus culture was incorporated into the laddu in three forms: lyophilized, microencapsulated powder, and natural curd. The probiotic foxtail laddu was selected based on specific criteria such as color, odor, and texture. The nutritional analysis revealed that the laddu contained approximately 64.46 g of carbohydrates, 15.13 g of protein, and 5.06 g of fat per 100 g of laddu. A microbial count analysis was performed over a two-month storage period to assess the viability of the incorporated Lactobacillus acidophilus. The results showed that the lyophilized and microencapsulated culture demonstrated good viability, with counts of 6.10 ± 0.09 log CFU/g and 7.43 ± 0.02 log CFU/g, respectively, when stored at 4 °C. In comparison, storage at room temperature resulted in counts of 5.41 ± 0.08 log CFU/g and 6.97 ± 0.02 log CFU/g at the end of the storage period. Based on the findings, the probiotic millet laddu developed in this study has the potential to be a value-added food product that can enhance the overall health of consumers. Incorporating probiotics into traditional food items like laddu offers a convenient and enjoyable way to promote gut health and improve the product's nutritional value.

Modeling and optimization of antibacterial effect of lichen-associated bacteria, Bacillus subtilis KSRLAB3 strain against marine fouling bacteria, Vibrio alginolyticus.

One of the most commonly occurring bacteria, Bacillus subtilis, can produce a wide variety of secondary metabolites. In this study, the antimicrobial effect of B. subtilis KSRLAB3 against Vibrio alginolyticus was optimized using the Plackett-Burman design (PBD) method, response surface methodology (RSM), and genetic algorithm (GA). Initially, the effects of carbon source, nitrogen source, NaCl concentration, pH, temperature, and incubation time on antimicrobial effects were studied. Among the carbon and nitrogen sources investigated, mannose and peptone elicited maximum antimicrobial effect. Using PBD, the most significant variables that influence the antimicrobial effect were identified, including incubation time, peptone concentration, and temperature. The optimum conditions required for attaining maximum antimicrobial effect was identified using the RSM-GA hybrid method, and the optimum condition includes 49.999 h of incubation time, 4.39 g/L of peptone concentration, and 27.629°C of incubation temperature. The confirmatory experiments performed around the optimum condition showed a zone of inhibition of 35 ± 0.52 mm. Methanolic extract also proved the presence of antibacterial lipopeptide surfactin. Therefore, the RSM-GA hybrid method was successfully used in this study to model the antimicrobial effect of B. subtilis KSRLAB3 against V. alginolyticus. The effective inhibition of V. alginolyticus can be investigated further for the development of antifouling coatings.

Synthesis and Characterization of Ferrous and Copper Nanoparticles from E-Waste Using Biological Reduction by Lichen-Associated Bacteria and Their Application in Antifouling Activity.

The largest and fastest growing industry in the world is electronic industries and the generation of waste are emerging problem. Electronic wastes are the source of precious metals that contributes 40 to 50 times more than the ore extracted from mines. The recycling of the waste is very important as it can protect the earth's natural resources. There are various methods for recycling e-waste such as chemical, fire, physical, and mechanical method. Currently, chemical treatment is in practice for recycling but, due to the usage of inorganic chemicals, it gives more environmental issues. Therefore, this paper used the biological method to prepare the nanoparticle from e-waste as it is an eco-friendly method. The copper and ferrous nanoparticle was extracted from the e-waste and biologically reduced using lichen-associated bacterial such as Parmotrema tintorum and P. recticulatum. The characteristics of these nanoparticles such as size, shape, and functional group were analyzed using UV, PSA, SEM, and FTIR respectively. The size of the synthesized particle was in the range of 10-100 nm using PSA. At the 2.5% concentration, the synthesis of ferrous nanoparticles was confirmed by the peak value obtained at 430 nm and 540 nm for copper nanoparticles. The antifouling properties of synthesized nanoparticles were analyzed by colliding them with the paint and applying to the iron surface. In recent research, the nanomaterials were able to use to reduce the fouling activity, also prevent harmful effects to the other marine species and the resistance of some microorganisms to antifoulants. This study helps to prevent environmental contaminants by using the copper and ferrous nanoparticle substances synthesized from the e-waste materials with the help of bacterial reduction.