Carrot Discard as a Promising Feedstock to Produce 2,3-Butanediol by Fermentation with P. polymyxa DSM 365.

The valorization of fruit and vegetable residues (such as carrot discard) and their microbial conversion into 2,3-butanediol (BDO) can be considered as a very interesting way to reduce food waste and sustainably originate high value-added products. This work analyzes the valorization of carrot discard as feedstock for 2,3-butanediol (BDO) production by Paenibacillus polymyxa DSM 365. The influences of stirring and the presence of tryptone (nitrogen source) are studied. Furthermore, in order to evaluate the influence of the pre-culture medium (nitrogen source, nutrients, and pH) and the substrate, fermentation assays in simple and mixture semi-defined media (glucose, fructose, and/or galactose) were also carried out. As a result, 18.8 g/L BDO, with a BDO yield of 0.43 g/g (86% of its theoretical value), could be obtained from carrot discard enzymatic hydrolysate at 100 rpm, no tryptone, and pre-culture Häßler medium. No hydrothermal pre-treatment was necessary for BDO production from carrot discard, which increases the profitability of the process. Therefore, 18.8 g BDO, as well as 2.5 g ethanol and 2.1 g acetoin by-products, could be obtained from 100 g of carrot discard (dry matter).

Efficient biobutanol production by acetone-butanol-ethanol fermentation from spent coffee grounds with microwave assisted dilute sulfuric acid pretreatment.

The integral valorization of potential sugars (cellulosic and hemicellulosic) from spent coffee grounds (SCG), a lignocellulosic residue, is proposed in this work. With this aim, the microwave assisted dilute sulfuric acid pretreatment has been optimized, leading to a hemicellulosic sugar recovery in the pretreatment liquid (HSRL) and an enzymatic hydrolysis yield of 79 and 98%, respectively, at 160.47 °C and 1.5% H2SO4. Moreover, the complete digestibility of cellulose (enzymatic hydrolysis yield = 100%) was also discovered for non-pretreated SCG, which is very interesting. Secondly, the production of biobutanol, an advanced biofuel, is also proposed from pretreated SCG enzymatic hydrolysate and pretreatment liquid achieved under optimal conditions. These were fermented by Clostridium beijerinckii, yielding 95 kg butanol/t SCG (dry matter) and 151 kg acetone-butanol-ethanol/t SCG (dry matter).

Efficient acetone-butanol-ethanol production by Clostridium beijerinckii from sugar beet pulp.

Sugar beet pulp (SBP) has been investigated as a promising feedstock for ABE fermentation by Clostridium beijerinckii. Although lignin content in SBP is low, a pretreatment is needed to enhance enzymatic hydrolysis and fermentation yields. Autohydrolysis at pH 4 has been selected as the best pretreatment for SBP in terms of sugars release and acetone and butanol production. The best overall sugars release yields from raw SBP ranged from 66.2% to 70.6% for this pretreatment. The highest ABE yield achieved was 0.4g/g (5.1g/L of acetone and 6.6g/L butanol) and 143.2g ABE/kg SBP (62.3g acetone and 80.9g butanol) were obtained when pretreated SBP was enzymatically hydrolyzed at 7.5% (w/w) solid loading. Higher solid loadings (10%) offered higher acetone and butanol titers (5.8g/L of acetone and 7.8g/L butanol). All the experiments were carried out under not-controlling pH conditions reaching about 5.3 in the final samples.

Acetone-butanol-ethanol (ABE) production by Clostridium beijerinckii from wheat straw hydrolysates: efficient use of penta and hexa carbohydrates.

ABE fermentation by Clostridium beijerinckii of steam-exploded and ozonated wheat straw hydrolysates was investigated. In steam-exploded hydrolysates, highest yields of 0.40 g/g ABE yield and 127.71 g ABE/kg wheat straw were achieved when the whole slurry from the pretreatment was used. In ozonated hydrolysates, 0.32 g/g ABE yield and 79.65 g ABE/kg wheat straw were obtained from washed ozonated wheat straw. Diverse effects were observed in steam explosion and ozonolysis of wheat straw which resulted in hemicellulose removal and acid insoluble lignin solubilization, respectively. SEM analysis showed structural differences in untreated and pretreated biomass. Depending on the operational strategy, after pretreatment and enzymatic hydrolysis, the glucose recovery ranged between 65.73-66.49% and 63.22-65.23% and the xylose recovery ranged between 45.19-61.00% and 34.54-40.91% in steam-exploded and ozonated hydrolysates, respectively. The effect of the main inhibitory compounds found in hydrolysates (oxalic acid, acetic acid, 5-hydroxymethylfurfural and furfural) was studied through ABE fermentation in model media.

Influence of aeration on bioethanol production from ozonized wheat straw hydrolysates using Pichia stipitis.

The influence of aeration on ethanol production by Pichia stipitis was studied in wheat straw hydrolysates subjected to ozone pretreatment for the first time. In a first stage, different aeration rates ranging from 0.03 to 0.50 L air/min, which corresponds to a volumetric oxygen transfer coefficient from 1.1 to 9.6 h(-1), were applied to model glucose/xylose substrates. The most promising value was found to be 3.3 h(-1) (0.1 L air/min) leading to better xylose utilization, an ethanol yield of 0.40 g ethanol/g sugars and complete depletion of sugars at 72 h. In a second stage, the effect of aeration was analyzed in ozonized wheat straw hydrolysates. Sugars were completely depleted at 96 h and ethanol yield reached a value of 0.41 g ethanol/g sugars. The addition of controlled oxygen (K(L)a=3.8 h(-1)) enhances the efficiency of the process causing an increase of 29.1% in ethanol production and a considerable reduction of 42.9% in fermentation time as compared to non-aerated hydrolysates.

Effect of inhibitors formed during wheat straw pretreatment on ethanol fermentation by Pichia stipitis.

The inhibitory effect of the main inhibitors (acetic acid, furfural and 5-hydroxymethylfurfural) formed during steam explosion of wheat straw was studied through ethanol fermentations of model substrates and hydrolysates from wheat straw by Pichia stipitis. Experimental results showed that an increase in acetic acid concentration led to a reduction in ethanol productivity and complete inhibition was observed at 3.5 g/L. Furfural produced a delay on sugar consumption rates with increasing concentration and HMF did not exert a significant effect. Fermentations of the whole slurry from steam exploded wheat straw were completely inhibited by a synergistic effect due to the presence of 1.5 g/L acetic acid, 0.15 g/L furfural and 0.05 g/L HMF together with solid fraction. When using only the solid fraction from steam explosion, hydrolysates presented 0.5 g/L of acetic acid, whose fermentations have submitted promising results, providing an ethanol yield of 0.45 g ethanol/g sugars and the final ethanol concentration reached was 12.2 g/L (10.9 g ethanol/100 g DM).