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1.
BJOG ; 129(5): 685-695, 2022 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-34559942

RESUMEN

BACKGROUND: Despite the existence of numerous published models predicting the risk of caesarean delivery in women undergoing induction of labour (IOL), validated models are scarce. OBJECTIVES: To systematically review and externally assess the predictive capacity of caesarean delivery risk models in women undergoing IOL. SEARCH STRATEGY: Studies published up to 15 January 2021 were identified through PubMed, CINAHL, Scopus and ClinicalTrials.gov, without temporal or language restrictions. SELECTION CRITERIA: Studies describing the derivation of new models for predicting the risk of caesarean delivery in labour induction. DATA COLLECTION AND ANALYSIS: Three authors independently screened the articles and assessed the risk of bias (ROB) according to the prediction model risk of bias assessment tool (PROBAST). External validation was performed in a prospective cohort of 468 pregnancies undergoing IOL from February 2019 to August 2020. The predictive capacity of the models was assessed by creating areas under the receiver operating characteristic curve (AUCs), calibration plots and decision curve analysis (DCA). MAIN RESULTS: Fifteen studies met the eligibility criteria; 12 predictive models were validated. The quality of most of the included studies was not adequate. The AUC of the models varied from 0.520 to 0.773. The three models with the best discriminative capacity were those of Levine et al. (AUC 0.773, 95% CI 0.720-0.827), Hernández et al. (AUC 0.762, 95% CI 0.715-0.809) and Rossi et al. (AUC 0.752, 95% CI 0.707-0.797). CONCLUSIONS: Predictive capacity and methodological quality were limited; therefore, we cannot currently recommend the use of any of the models for decision making in clinical practice. TWEETABLE ABSTRACT: Predictive models that predict the risk of cesarean section in labor inductions are currently not applicable.


Asunto(s)
Cesárea , Trabajo de Parto Inducido , Área Bajo la Curva , Estudios de Cohortes , Femenino , Humanos , Embarazo , Estudios Prospectivos
2.
Front Cell Dev Biol ; 9: 620730, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33718360

RESUMEN

Syndrome coronavirus 2 (SARS-CoV-2) pandemic is causing a second outbreak significantly delaying the hope for the virus' complete eradication. In the absence of effective vaccines, we need effective treatments with low adverse effects that can treat hospitalized patients with COVID-19 disease. In this study, we determined the existence of SARS-CoV-2-specific T cells within CD45RA- memory T cells in the blood of convalescent donors. Memory T cells can respond quickly to infection and provide long-term immune protection to reduce the severity of COVID-19 symptoms. Also, CD45RA- memory T cells confer protection from other pathogens encountered by the donors throughout their life. It is of vital importance to resolve other secondary infections that usually develop in patients hospitalized with COVID-19. We found SARS-CoV-2-specific memory T cells in all of the CD45RA- subsets (CD3+, CD4+, and CD8+) and in the central memory and effector memory subpopulations. The procedure for obtaining these cells is feasible, easy to implement for small-scale manufacture, quick and cost-effective, involves minimal manipulation, and has no GMP requirements. This biobank of specific SARS-CoV-2 memory T cells would be immediately available "off-the-shelf" to treat moderate/severe cases of COVID-19, thereby increasing the therapeutic options available for these patients.

3.
Nano Lett ; 20(9): 6372-6380, 2020 Sep 09.
Artículo en Inglés | MEDLINE | ID: mdl-32786947

RESUMEN

A damping-like spin-orbit torque (SOT) is a prerequisite for ultralow-power spin logic devices. Here, we report on the damping-like SOT in just one monolayer of the conducting transition-metal dichalcogenide (TMD) TaS2 interfaced with a NiFe (Py) ferromagnetic layer. The charge-spin conversion efficiency is found to be 0.25 ± 0.03 in TaS2(0.88)/Py(7), and the spin Hall conductivity (14.9×105ℏ2eΩ-1m-1) is found to be superior to values reported for other TMDs. We also observed sizable field-like torque in this heterostructure. The origin of this large damping-like SOT can be found in the interfacial properties of the TaS2/Py heterostructure, and the experimental findings are complemented by the results from density functional theory calculations. It is envisioned that the interplay between interfacial spin-orbit coupling and crystal symmetry yielding large damping-like SOT. The dominance of damping-like torque demonstrated in our study provides a promising path for designing the next-generation conducting TMD-based low-powered quantum memory devices.

4.
HLA ; 2018 Apr 25.
Artículo en Inglés | MEDLINE | ID: mdl-29692004

RESUMEN

Five new HLA class I alleles are described, A*30:129, B*08:195, B*51:01:62, C*01:147 and C*12:195:02.

5.
Rev Clin Esp (Barc) ; 218(4): 192-198, 2018 May.
Artículo en Inglés, Español | MEDLINE | ID: mdl-29519537

RESUMEN

This positioning document describes the most important aspects of clinical ultrasonography in the internal medicine setting, from its fundamental indications to the recommended training period. There is no question as to the considerable usefulness of this tool in the standard clinical practice of internists in numerous clinical scenarios and settings (emergencies, hospital ward, general and specific consultations and home care). Ultrasonography has a relevant impact on the practitioner's ability to resolve issues, increasing diagnostic reliability and safety and providing important information on the prognosis and progression. In recent years, ultrasonography has been incorporated as a tool in undergraduate teaching, with excellent results. The use of ultrasonography needs to be widespread. To accomplish this, we must encourage structured training and the acquisition of equipment. This document was developed by the Clinical Ultrasonography Workgroup and endorsed by the Spanish Society of Internal Medicine.

6.
HLA ; 91(4): 313-314, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29388731

RESUMEN

A new DRB1*07 allele, DRB1*07:83, was described in a Caucasian Spanish donor.


Asunto(s)
Alelos , Cadenas HLA-DRB1/genética , Secuencia de Bases , Exones/genética , Prueba de Histocompatibilidad , Humanos , Alineación de Secuencia
9.
Rev Clin Esp (Barc) ; 217(5): 245-251, 2017.
Artículo en Inglés, Español | MEDLINE | ID: mdl-28318521

RESUMEN

OBJECTIVE: To analyse the ability of medical students to incorporate the practical teaching of basic echocardiography planes using a peer mentoring design. METHODOLOGY: Thirty-six medical students previously trained in obtaining echocardiography planes (mentors) taught the other 5th-year students (n=126). The teaching methodology included three stages: theory (online course), basic training (three 15h sessions of practical experience in ultrasound and at least 20 echocardiographic studies per mentor) and objective structured clinical assessment (OSCA), which scored the appropriateness of the basic ultrasound planes and the correct identification of 16 cardiac structures. RESULTS: The students' weighted mean score in the OSCA was 8.66±1.98 points (out of 10). Only 10 students (8.4%) scored less than 5, and 15 (12.6%) scored less than 7. Fifty students (42%) scored 10 points. The most easily identified structure was the left ventricle in the short-axis parasternal plane, with 89.9% of correct answers. The most poorly identified structure was the mitral valve in the subxiphoid plane, with 69.7% of correct answers. CONCLUSIONS: Peer mentoring-based teaching achieves an appropriate level of training in obtaining basic echocardiography planes. The training period is relatively short. The peer mentoring system can facilitate the implementation of teaching on basic aspects of ultrasound to a large number of undergraduate students.

10.
HLA ; 89(4): 230-234, 2017 04.
Artículo en Inglés | MEDLINE | ID: mdl-28205408

RESUMEN

BACKGROUND: The assignment of human leukocyte antigen (HLA) null alleles is clinically relevant in the setting of stem cell transplantation. Cell surface expression profiling and mRNA processing analysis of the HLA-B allele previously designated as B*07:44, have been performed. MATERIALS AND METHODS: Cell surface expression of HLA-B*07:44 was determined using flow cytometry. Genomic full-length and HLA-B*07-specific cDNA sequencing were carried out by Sanger procedure. RESULTS: Flow cytometric analysis confirmed previous serologic results and demonstrated a lack of cell membrane expression of the HLA-B protein. The mRNA processing, studied using direct HLA-B*07-specific cDNA sequencing, revealed the presence of a unique, aberrantly spliced mRNA, with a deletion of the last 43 bp on the 5'-end of exon 4. The substitution from T to G at genomic position 1799 compared to B*07:02:01 introduced a new and stronger splice donor site at exon 4. This alternative splicing produced an mRNA containing a premature stop codon at position 280, explaining the absence of mature HLA-B7 protein on the cell surface. CONCLUSION: These findings led us to consider this HLA-B variant as a HLA null allele. The World Health Organization (WHO) Nomenclature Committee has since renamed this variant B*07:44N .


Asunto(s)
Empalme Alternativo , Secuencia de Bases , Antígeno HLA-B7/genética , ARN Mensajero/genética , Eliminación de Secuencia , Alelos , Trasplante de Médula Ósea , Clonación Molecular , Codón de Terminación , ADN Complementario/genética , ADN Complementario/inmunología , Exones , Citometría de Flujo , Antígeno HLA-B7/inmunología , Humanos , ARN Mensajero/inmunología , Alineación de Secuencia , Análisis de Secuencia de ADN , Terminología como Asunto , Donantes de Tejidos
16.
Tissue Antigens ; 85(2): 141-2, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25626608

RESUMEN

HLA-B*18:105 shows two nucleotide differences regarding B*18:22 (97 AGC>AGG, 99 TAC>TAT) and B*18:52 (94 ACC>ATC, 95 CTC>ATC).


Asunto(s)
Alelos , Antígenos HLA-B/genética , Hispánicos o Latinos/genética , Población Blanca/genética , Secuencia de Bases , Exones/genética , Humanos , Datos de Secuencia Molecular , Alineación de Secuencia
17.
Plant Physiol Biochem ; 83: 308-15, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-25218731

RESUMEN

Scarcity of water is a severe limitation in citrus tree productivity. There are few studies that consider how to manage nitrogen (N) nutrition in crops suffering water deficit. A pot experiment under controlled-environment chambers was conducted to explore if additional N supply via foliar application could improve the drought tolerance of Citrus macrophylla L. seedlings under dry conditions. Two-month-old seedlings were subjected to a completely random design with two water treatments (drought stress and 100% water/field capacity). Plants under drought stress (DS) received three different N supplies via foliar application (DS: 0, DS + NH4NO3: 2% NH4NO3, DS + KNO3: 2% KNO3). KNO3-spraying increased leaf and stem DW as compared with DS + NH4NO3 and DS treatments. Leaf water potential (Ψw) was decreased by drought stress in all the treatments. However, in plants from DS + NH4NO and DS + KNO3, this was due to a decrease in the leaf osmotic potential, whereas the decrease in those from the DS treatment was due to a decrease in the leaf turgor potential. These responses were correlated with the leaf proline and K concentrations. DS + KNO3-treated plants had a higher leaf proline and K concentration than DS-treated plants. In terms of leaf gas exchange parameters, it was observed that net assimilation of CO2 [Formula: see text] was decreased by drought stress, but this reduction was much lower in DS + KNO3-treated plants. Thus, when all results are taken into account, it can be concluded that a 2% foliar-KNO3 application can enhance the tolerance of citrus plants to water stress by increasing the osmotic adjustment process.


Asunto(s)
Citrus/metabolismo , Sustancias Explosivas/farmacología , Nitratos/farmacología , Hojas de la Planta/metabolismo , Compuestos de Potasio/farmacología , Plantones/genética , Estrés Fisiológico/efectos de los fármacos , Sequías
19.
Tissue Antigens ; 80(6): 541-2, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23033953

RESUMEN

A*29:39 differs from A*29:02:01 by three clustered amino acid replacements at α1 domain, T73>I73, A76>V76 and N77>D77. A*29:40 shows one nucleotide difference regarding A*29:02:01 allele, resulting in one amino acid substitution at position 154, E154>G154.


Asunto(s)
Antígenos HLA-A/genética , Alelos , Secuencia de Aminoácidos , Exones , Técnicas de Genotipaje , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Homología de Secuencia de Aminoácido
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