Individual and combined impact of microplastics and lead acetate on the freshwater shrimp (Caridina fossarum): Biochemical effects and physiological responses.

Microplastics and heavy metals pollution is recognised as a major problem affecting aquatic ecosystems. For this reason, this study aims to assess the toxicity of different concentrations of polyethylene microplastics (PE-MPs) (0.0, 500, and 1000 µg L-1) with a mean size of 15-25 µm and lead acetate Pb(C2H3O2)2 (0.0, 2.5, and 5 mg L-1), both individually and in combination, through the exposure of the freshwater grass shrimp, Caridinia fossarum for 15 days, focusing on microplastic interaction with co-occurring contaminants. After being exposed to both contaminants, either individually or in combination, significant alterations in numerous biochemical markers were observed. Specifically, exposure to lead acetate alone resulted in significant changes across ALP, AST, ALT, LDH, GGT, and BChE enzyme activity levels indicating hepatotoxicity and neurotoxicity. Also, Pb exposure led to alterations in total antioxidant capacity, MDA, total lipids, and glycogen contents, signalling the onset of oxidative stress. Exposure to PE-MPs alone led to changes in ALP, LDH, GGT, and BChE enzyme levels, and in MDA, total lipids, and glycogen samples' contents. Remarkably, the study observed increased bioaccumulation of lead acetate in samples treated with the combination, emphasizing the synergistic impact of PE-MPs on the toxicity of lead acetate. This synergy was also evident in AST and ALT enzyme activity levels and MDA contents. This underscores the necessity for measures to address both microplastic pollution and heavy metal contamination, taking into account the synergistic behaviour of MPs in the presence of concurrent contaminants.

Dietary Mentha piperita essential oil loaded in chitosan nanoparticles mediated the growth performance and humoral immune responses in Siberian sturgeon (Acipenserbaerii).

Siberian sturgeon (Acipenser baerii) fry often face environmental stressors that can compromise their immune system, rendering them susceptible to opportunistic pathogens in intensive aquaculture systems. In this study, we explored the innovative use of chitosan nanoparticles loaded with Mentha piperita essential oil (MPO/CNPs) as a dietary supplement to improve the growth and immune responses of A. baerii. The results demonstrated that the addition of MPO/CNPs to the diet led to significant improvements in growth, as evidenced by increased red blood cell count, hematocrit, haemoglobin concentration, and reduced triglyceride levels. Furthermore, significant differences were observed in the immune parameters for the treatment groups receiving Mentha piperita essential oil loaded in chitosan nanoparticles (MPO/CNPs), including enhanced lysozyme activity, immunoglobulin M (IgM) levels, respiratory burst activity, and ACH50 activity. Additionally, gene expression analysis revealed upregulation of key immune-related genes in the MPO/CNPs-treated groups. These findings suggest that the use of MPO/CNPs can enhance the growth and bolster the immune defences of Siberian sturgeon fry, contributing to more sustainable production in intensive aquaculture environments.

Single and combined effects of CuSO4 and polyethylene microplastics on biochemical endpoints and physiological impacts on the narrow-clawed crayfish Pontastacusleptodactylus.

This study investigated the toxicity of polyethylene microplastics (MPs; <0.02 mm) and CuSO4, alone and in combination, on the freshwater crayfish Pontastacus leptodactylus. In this study, the crayfish were exposed to PE-MPs (0.0, 0.5, and 1 mg L-1) and CuSO4·5H2O (0.0, 0.5, and 1 mg L-1) for a period of 28 days. Next, multi-biomarkers, including biochemical, immunological, and oxidative stress indicators were analyzed. Results showed that co-exposure to PE-MPs and CuSO4 resulted in increased aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and decreased alkaline phosphatase (ALP), butyrylcholinesterase (BChE), and gamma-glutamyl-transferase (GGT). Triglycerides, cholesterol, glucose, and albumin content also increased. Although no significant change was observed in lysozyme and phenoloxidase activities in crayfish co-exposed to 0.5 mg L-1 MPs and 0.5 mg L-1 CuSO4, their activities were significantly decreased in other experimental groups. Oxidative stress parameters in hepatopancreas indicated increased superoxide dismutase (SOD), glutathione peroxidase (GPx), and in malondialdehyde (MDA) levels, but decreased catalase (CAT), glucose 6-phosphate dehydrogenase (G6PDH), and cellular total antioxidant (TAC). Results showed that the sub-chronic toxicity of CuSO4 was confirmed. The study confirmed the toxicity of CuSO4 and found that higher concentrations led to more severe effects. Co-exposure to PE-MPs and CuSO4 primarily compromised the endpoints, showing increased toxicity when both pollutants were present in higher concentrations. The activities of POX, LYZ, ALP, GGT, LDH, and CAT were suppressed by both CuSO4 and MPs. However, a synergistic increase was observed in other measured biomarkers in crayfish co-exposed to CuSO4 and MPs.

Physiological response of freshwater crayfish, Astacus leptodactylus exposed to polyethylene microplastics at different temperature.

Not long after plastic-made material became an inseparable part of our lives, microplastics (MPs) found their way into ecosystems. Aquatic organisms are one of the groups impacted by man-made materials and plastics; however, the varied effects of MPs on these organisms have yet to be fully understood. Therefore, to clarify this issue, 288 freshwater crayfish (Astacus leptodactylus) were assigned to eight experimental groups (2 × 4 factorial design) and exposed to 0, 25, 50, and 100 mg polyethylene microplastics (PE-MPs) per kg of food at 17 and 22 °C for 30 days. Then samples were taken from hemolymph and hepatopancreas to measure biochemical parameters, hematology, and oxidative stress. The aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and catalase activities increased significantly in crayfish exposed to PE-MPs, while the phenoxy-peroxidase, gamma-glutamyl peptidase, and lysozyme activities decreased. Glucose and malondialdehyde levels in crayfish exposed to PE-MPs were significantly higher than in the control groups. However, triglyceride, cholesterol, and total protein levels decreased significantly. The results showed that the increase in temperature significantly affected the activity of hemolymph enzymes, glucose, triglyceride, and cholesterol contents. The semi-granular cells, hyaline cells, granular cell percentages, and total hemocytes increased significantly with the PE-MPs exposure. Temperature also had a significant effect on the hematological indicators. Overall, the results showed that temperature variations could synergistically affect the changes induced by PE-MPs in biochemical parameters, immunity, oxidative stress, and the number of hemocytes.

The effects of Shirazi thyme (Zataria multiflora) oil nanoemulsion on the quality of shrimp (Litopenaeus vannamei) during refrigerated storage.

The effects of carboxymethyl cellulose (CMC) coating incorporated with Shirazi thyme (Zataria multiflora) oil nano emulsion (SNE), in different concentrations (10, 20, and 30 mg/ml), on the melanosis and the quality of Pacific white shrimp (Litopenaeus vannamei) was investigated during 10 days in refrigerated temperature (4 ± 0.5 °C). The results showed that incorporating SNE into CMC could significantly (P < 0.05) improve the microbial and lipid oxidation quality of the shrimp. During storage, the incremewnt of total volatile basic-nitrogen and trimethylamine in the SNEs-treated groups were lower than that of the other groups (P < 0.05). Also, the application of SNEs improved the textural, melanosis, and sensory acceptability of the coated shrimps. However, treating the shrimp with SNE in 30 mg/ml concentration caused an increase in the a* and b* values of samples and a decrease in the acceptability of this group. Hence, the SNE incorporation at lower concentrations (10, 20 mg/ml) into CMC coating could be useful in extending the shelf life of the shrimp during refrigerated storage and could be a substitute for sodium metabisulphite.

Investigation of Different Nutritional Effects of Dietary Chromium in Fish: A Literature Review.

The supply of food for the world population that is increasing is one of the concerns of governments. The Food and Agriculture Organization of the United Nations assessment shows that the aquaculture industry could help meet food needs for human communities. The aquaculture industry also relies on providing a feed of high quality. Minerals are one essential component of an aquatic diet. Chromium (Cr) is a trace element that finds the form of Cr+3 (trivalent) and Cr+6 (hexavalent) in nature and food items. Studies show that exposure to Cr waterborne have toxicity effects on fish. However, oral exposure to Cr has a different impact on fish. Cr is usually involved in the metabolism of fats, carbohydrates, proteins, growth function, enzyme functions, etc. This element could play a significant role in fish nutrition and physiology. Cr as a dietary supplement can improve growth performance and adjust the metabolism of carbohydrates and lipids. However, high concentrations of Cr can be toxic to fish. Although the physiological effects of Cr on aquatic organisms are well known, there are still ambiguities in determining the appropriate concentration in the diet of some species. Maybe, the physiological response of fish depends on the concentration, origin, and chemical composition of Cr, as well as the biological and individual characteristics of the fish. Therefore, it is necessary to estimate the appropriate concentration of Cr in fish diets. This article aims to summarize the available information about the effect of Cr on various physiological indicators and fish growth. Therefore, this information may help to find the appropriate concentration of Cr in the diet.

Effect of microplastics on Yersinia ruckeri infection in rainbow trout (Oncorhynchus mykiss).

Exposure to microorganisms such as Yersinia ruckeri can significantly affect bacterial infections in fish. Microplastics (MPs) may predispose fish to infection and act as carriers in pathogen transmission. Therefore, this study is designed to evaluate MPs' effect on damage caused by exposure to Y. ruckeri in rainbow trout. In this study, blood biochemical parameters and hepatic oxidative biomarkers as clinical signs were measured in the fish co-exposed to Y. ruckeri (5 and 10% the median lethal dose (LD50)) and MPs (500 and 1000 mg Kg-1) for 30 days. There were no significant changes in the creatinine, triglyceride, cholesterol levels, and glutamic-pyruvic transaminase activity in the blood of fish infected with Y. ruckeri. In contrast, exposure to MPs had a significant effect on most clinical parameters. The total protein, albumin, globulin, total immunoglobulins, high-density lipoprotein, low-density lipoprotein, cholesterol levels, and γ-glutamyltransferase activity decreased, whereas glucose, triglyceride, and creatinine levels, and glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase, alkaline phosphatase, and lactate dehydrogenase activities increased in the plasma of fish after co-exposure to MPs and Y. ruckeri. Dietary MPs combined with a Y. ruckeri challenge decreased catalase and glutathione peroxidase activities, and total antioxidant levels. However, superoxide dismutase activity and malondialdehyde contents increased in the hepatocyte of fish co-exposed to MPs and Y. ruckeri. This study suggests that fish exposure to MPs and simultaneous challenge with Y. ruckeri could synergistically affect clinical parameters.

Heavy Metals in the Blood Serum and Feces of Mugger Crocodile (Crocodylus palustris) in Sistan and Baluchistan Province, Iran.

This study was conducted to measure concentrations of cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg), copper (Cu), chromium (Cr), and iron (Fe) in blood serum and feces of mugger crocodile (Crocodylus palustris) and in water and sediment of their habitat (Negour site, Chabahar County in Sistan and Baluchistan Province, Iran). Heavy metal contents in crocodile serum and feces, water, and sediments were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES spectrometer-Spectro Genesis). The highest levels of heavy metals in the mugger crocodile's serum were Fe > Hg > Pb > Cr > Cu > Cd > As, respectively. The total metal concentrations in the feces exhibited the following decreasing order from Fe > As > Pb = Cr > Hg > Cd > Cu. The highest average levels of heavy metals in water were Hg > Fe > As > Pb > Cu > Cr > Cd respectively and As > Fe > Pb > Cu > Hg > Cd > Cr in the sediment of lagoons. A significant difference was observed in the concentration of Fe and Pb between male and female crocodiles. Moreover, correlation analysis indicated significant negative correlations between sex and bioconcentrations of Cd, Fe, Pb, As, and Hg in the serum. In conclusion, this study showed that the environmental conditions of Negour lagoons are not suitable for crocodiles regarding habitat health and water quality. Furthermore, since these lagoons and fish are the most important sources of drinking water and seafood for the residents of the area, contamination of these pools with heavy metals can also threaten people's health.

A study on the oral and cloacal bacterial flora of Mugger crocodiles (Crocodylus palustris) in the Negour protected area, Iran.

Mugger crocodile is the only crocodile existing in Iran. The present study was aimed to investigate the bacterial flora in oral and cloacal cavities of wild Mugger crocodiles in Negour protected area, Iran. The isolation and molecular characterization of oral and cloacal bacterial flora were performed in 22 Mugger crocodiles captured in Negour protected area, Iran. Ten bacterial species from all oral samples and six bacterial species from all cloacal samples were recovered. The most commonly isolated bacteria in oral samples were Burkholderia contaminans and Lactococcus garvieae, respectively; whereas, in cloacal samples, it was Lactococcus lactis. It is likely that the isolated bacteria would pose a threat to both crocodiles and humans health. It can threaten crocodiles during stressful conditions; while, humans would be susceptible if they are bitten by crocodiles, consume their meat or spend time near their natural environment. This study provides useful information about bacterial diversity which could help to select the most appropriate anti-bacterial when dealing with infections caused by crocodiles.

Effects of microplastic exposure on the blood biochemical parameters in the pond turtle (Emys orbicularis).

The accumulation of microplastics (MPs) is a growing problem in aquatic ecosystems. Despite increased research on MPs in the last decade, their potential threat to freshwater ecosystems remains an open question. In the present study, the negative impacts of MPs were investigated on blood biochemical parameters in the European pond turtle (Emys orbicularis). Pond turtles were distributed into three experimental groups (n = 9 for each group) and were fed diets containing 250, 500, and 1000 mg MPs (PE100 polyethylene) per kg of food for 30 days, and a control group fed with a standard uncontaminated diet. The results indicated that exposure to 500 and 1000 mg kg-1 MPs caused a significant increase in the activities of alanine and aspartate aminotransferases, and in the levels of cholesterol, glucose, creatinine, urea, and calcium (Ca+2) compared with the control group. On the contrary, the activity of gamma-glutamyl transferase and the levels of total protein, albumin, total immunoglobulins, and phosphorus were significantly reduced in E. orbicularis exposed to 500 and 1000 mg kg-1 MPs when compared with the controls. In all the MP-exposed groups, the activity of lactate dehydrogenase and globulin and magnesium (Mg+2) levels were significantly reduced; while creatine phosphokinase and alkaline phosphatase activities were increased with respect to the control turtles. A significant decrease in triglyceride levels was reported in E. orbicularis exposed to 1000 mg kg-1 MPs. MPs intake induced notable alterations in blood biochemical parameters of E. orbicularis. These results suggest that changes in the blood biochemical parameters could be an appropriate bio-indicator to evidence the existence of tissue damage in E. orbicularis.

Combined effects of exposure to sub-lethal concentration of the insecticide chlorpyrifos and the herbicide glyphosate on the biochemical changes in the freshwater crayfish Pontastacus leptodactylus.

Glyphosate is an herbicide that inhibits the growth of weed plants, while chlorpyrifos is an insecticide commonly applied to control the pests' population. This study aimed to investigate the combined effects of chlorpyrifos and glyphosate on biochemical, immunological parameters, and oxidative stress biomarkers in freshwater crayfish Pontastacus leptodactylus for 21 days. The experimental design of this study was factorial (3 × 3), including 0.0, 0.4, and 0.8 mg L-1 glyphosate and 0.0, 2.5, and 5 µg L-1 chlorpyrifos. The exposure to chlorpyrifos, glyphosate alone and a mixture of them significantly decreased acetylcholinesterase, alkaline phosphatase, phenoloxidase activities, and total protein levels. The lactate dehydrogenase, glutamic-pyruvic-transaminase, and catalase activities, the contents of glucose, and malondialdehyde levels were increased in the crayfish. No significant changes were detected in glutamic-oxaloacetic-transaminase (SGOT) activity, triglyceride, and total antioxidant (TAO) levels in the crayfish treated with 0.4 mg L-1 glyphosate and the control group. Co-exposure of crayfish to chlorpyrifos and glyphosate increased SGOT activity and TAO levels. Although chlorpyrifos combined with glyphosate decreased the γ-Glutamyltransferase (GGT) activity, the GGT activity was significantly increased in the P. leptodactylus exposed during 21 days to 5 µg L-1 chlorpyrifos alone and 0.8 mg L-1 glyphosate alone. In comparison with the reference group, no significant changes were evidenced in the cholesterol levels in the P. leptodactylus treated with 2.5 µg L-1 chlorpyrifos, but its levels were significantly increased in the other treatment groups. In conclusion, the mix of glyphosate and chlorpyrifos exhibited synergic effects on the different toxicological biomarkers in the narrow-clawed crayfish. Co-exposure to pesticides may result in disruption of homeostasis in the crayfish by altering the biochemical and immunological parameters.

Molecular, histopathologic and electron microscopic analysis of white spot syndrome virus in wild shrimp (Fenneropenaeus indicus) in the coastal waters of Iran.

So far, there have been no studies on the distribution of viral white spot syndrome in wild Indian white shrimp (Fenneropenaeus indicus) brooders at Iranian capture sites. This study was conducted to investigate the presence of white spot syndrome virus (WSSV) in wild Indian white shrimps in Iran, using PCR, histopathologic, and electron microscopic surveys. The samples were collected within two seasons (autumn and spring) and from two provinces (six capture sites), from the major hatcheries providing spawners. Eight hundred thirty-three samples were collected and analyzed first by PCR, after which the positive samples were examined using histological tests, and if inclusion bodies were observed, electron microscopy was also used. White spot syndrome virus was detected only at the capture sites in Sistan and Baluchistan Province, where the mean infection rate was significantly higher in the spring (8.7%) than in the autumn (2.03%). At the Chabahar, Pasabandar, and Govater capture sites, the mean infection rate was significantly higher (4.9%, 2.1%, and 9.2%, respectively), than in Hormozgan Province. The results showed that there was no significant difference in infection rate between the two different sizes and sexes of shrimps (P < 0.05). Phylogeny analysis revealed a close relationship between the viruses from this study and those in other Asian countries, including China, India, Bangladesh, Thailand, Taiwan, and South Korea. It is possible that the virus has spread across the Indian Ocean to other countries. Therefore, the spawners in this study, particularly those collected during the spring and those from capture sites in Sistan and Baluchistan Province, were found to be more susceptible to WSSV infection, and the virus might have been transmitted vertically from WSSV-infected brooders to post-larvae.

A bioassay on tissue cholinesterase activity of Rutilus kutum (Kamensky, 1901) exposed to some common pesticides in Iran.

The toxicological effects of three commonly used pesticides in Iran on the fries of Caspian kutum (Rutilus kutum, Kamensky, 1901) were investigated through determining 50.00% lethal concentration (LC50) 96hr and cholinesterase (ChE) inhibition. The LC50 96-hr of carbaryl, glyphosate, and malathion were equal to 11.69, 6.64, and 0.97 mg L-1, respectively, that were considered as harmful (10.00 - 100 mg L-1), toxic (1.00 - 10.00 mg L-1) and very toxic (< 1.00 mg L-1) compounds for this species. The exposure of fries to sub-lethal concentrations of the pesticides over 15 days indicated that the average of ChE activity in the head and trunk were 1086.89 ± 124.34 and 627.36 ± 99.60 mU min-1 per mg protein, respectively, with a significant difference relative to each other. There was a significant difference between fry exposed to all three pesticides and the control group in cholinesterase inhibition. The fries exposed to carbaryl (890.12 ± 28.08 mU min-1 per mg protein) and glyphosate (891.77 ± 31.61 mU min-1 per mg protein) showed lower ChE inhibition than those exposed to malathion (790.00 ± 58.14 mU min-1 per mg protein).

Evaluation of single and combined effects of cadmium and micro-plastic particles on biochemical and immunological parameters of common carp (Cyprinus carpio).

The growing accumulation of microplastics (MPs) in aquatic environments is a global concern. MPs are capable to interact with other environmental contaminants, including heavy metals, altering their toxicity. The aim of the study was to investigate the sub-lethal effects of cadmium chloride (Cd) alone and in combination with MPs on common carp (Cyprinus carpio). Multi-biomarkers, including plasma biochemical parameters and intrinsic immunological factors, were measured after 30 days of exposure. Exposure to Cd or NPs reduced the plasma activities of acetylcholinesterase (AChE) and gamma-glutamyl-transferase (GGT) and increased aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP). Exposure to both compounds enhanced the observed effects except for AST activity and ALP at the highest concentrations, whereas evidenced an antagonistic interaction in ALT. Plasma total protein, albumin, and globulin levels were decreased, and the levels of glucose, triglyceride, and cholesterol levels increased mainly in the Cd groups with no additional effects derived from the co-exposure to both stressors. Lysozyme and alternative complement (ACH50) activities and the levels of total immunoglobulins, and complement C3 and C4 in fish exposed to Cd and MPs were lower than those in the control group and this decrease was more significant by the mixture of both compounds. These findings showed that the exposure to Cd or MPs alone is toxic to fish altering the biochemical and immunological parameters. Moreover, these alterations are even greater when the Cd and the MPS are combined suggesting synergistic effects in increasing Cd toxicity and vice versa.

Acute exposure to chlorpyrifos and glyphosate induces changes in hemolymph biochemical parameters in the crayfish, Astacus leptodactylus (Eschscholtz, 1823).

Acute and subacute toxicity tests for chlorpyrifos and glyphosate were performed on the crayfish, Astacus leptodactylus. The crayfish were divided into a control group and four experimental groups with exposure from 50.18 to 301.11 µg L-1 of chlorpyrifos for 96 h under semi-static test conditions. In the same experiment, the crayfish were exposed to different concentrations of glyphosate (from 0.0, 8.14 to 13.05 mg L-1) for 96 h. Mortality was recorded, and the median lethal concentrations (LC50) were calculated using probit analysis. The 96 h LC50 values of chlorpyrifos and glyphosate to A. leptodactylus were 49.55 ±â€¯4.66 µg L-1 and 7.83 ±â€¯0.50 mg L-1, respectively. The results showed that chlorpyrifos was a few hundred times more toxic than glyphosate for the crayfish. Then, the crayfish were exposed to different subacute concentrations of chlorpyrifos (12.5 and 25 µg L-1) and glyphosate (2 mg L-1 and 4 mg L-1) for seven days, and their toxicity was evaluated through several hemolymph biochemical parameters. Chlorpyrifos and glyphosate exposure caused a significant (P < 0.01) increase in the activity of lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT). The results showed a significant (P < 0.01) decrease in acetylcholinesterase (AChE), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and phenoloxidase (PO) activities in the hemolymph of the crayfish exposed to chlorpyrifos and glyphosate. The total protein and cholesterol levels showed a significant decrease (P < 0.01) in the hemolymph of the crayfish after seven days of exposure to chlorpyrifos and glyphosate. There was a significant increase in triglycerides and glucose levels in treated crayfish. These results show that chlorpyrifos is highly toxic, while glyphosate is moderately toxic to A. leptodactylus. According to the results, subacute concentrations of chlorpyrifos and glyphosate could lead to a collapse of cellular homeostasis and changes in the hemolymph biochemical parameters in the crayfish. Physiological changes caused by exposure to chlorpyrifos and glyphosate in A. leptodactylus may have direct effects on the survival of these organisms.

First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran.

The purpose of the present study was to isolate and identify the pathogenic agents in Acipenser stellatus (Pallas, 1771) and Huso huso, (Linnaeus, 1758) reared in the south of Fars province, Iran which have shown infectious disease signs. Samples from spleen and kidney of 32 fishes showing septicemia symptoms such as decreasing of appetite, unbalanced swimming, expanded wounds, and petechia on the body surfaces, pectoral fins rot, visceral hemorrhage, bleeding on the spleen, and heart ascites were collected. Then samples were cultured on brain heart infusion agar growth media, stain and biological and biochemical tests on purified bacteria were performed. On the other hand, 16S rDNA region of the isolated organism was amplified using PCR. The amplified gene fragment was sequenced and evolutionary history was inferred by phylogenetic tree construction using neighbor-joining method. Results indicated that two bacterial species including Chryseobacteriumjoostei which isolated from the kidney of stellate sturgeon (43.00%), and Aeromonasveronii which isolated from the spleen of both sturgeon species (75.00% and 31.00% from beluga and stellate sturgeon, respectively), were recognized. Phylogenetic tree analysis showed that Fars isolated organisms including A. veronii and C. joostei had highest similarity with A. veronii bv veronii and C. joostei isolated from France, respectively.

Modulation of rainbow trout (Oncorhynchus mykiss) cutaneous mucosal immune responses following anesthesia: A comparative study on different anesthetic agents.

The present study investigated the possible effects of different anesthetic agents including MS222 (50 ppm), 2-Phenoxyethanol (2-PE) (0.2 mL L-1) and clove oil (25 ppm), on cutaneous mucosal immune parameters in rainbow trout (Oncorhynchus mykiss). The induction and recovery times for each anesthetic agent were assessed. Also, the immune parameters were measured in skin mucus, 1 and 24 h post anesthesia. No significant difference was observed among treatments at 1 h post-anesthesia except for bactericidal and alkaline phosphatase (ALP) activities which was significantly enhanced in fish exposed to 2-PE compared to other anesthetics. At 24 h post-anesthesia, most of the skin mucosal immune parameters were increased upon exposure to clove oil but decreased following exposure to 2-PE. However, no significant change was noticed after MS222 exposure. These results demonstrated that the anesthetics type should be considered to avoid possible immunosuppression in farmed fish. Furthermore, the present results could be useful for better understanding of alterations in cutaneous mucosal immunity in response to chemical stressors such as anesthetic agents.

Endohelminths of European pond turtle Emys orbicularis in Southwest Iran.

Very little is known about parasitic diseases of European pond turtles (Emys orbicularis) in Iran. The objective of this study is to examine parasitic fauna of European pond turtles collected from Fars province, southwest Iran. Carcasses of turtles (n = 52) which died during dredging procedure are collected from earthen fishery basins in Zarghan region. They have been died earlier during dredging procedure in different farms. Three species of helminths in total were found in gastrointestinal tract, including two nematodes (Serpinema microcephalus and Falcaustra araxiana) and one digenean trematod (Telorchis assula). Large intestines of all examined turtles were infected by F. araxiana (100 %, Mean intensity = 18) and this nematode were also found in gastric nodules. Nine turtles (17.3 %, 3 male, 6 female, Mean intensity = 3) were infected with Serpinema microcephalus. T. assula were found in 25 turtles (48.07 %, 5 male, 20 female, mean intensity = 5). Helminths were not found in any examined organs and no ectoparasite found eighter. F. araxiana is the most prevalent nematode in European pond turtles. Detection of Serpinema.microcephalus is in agreement with the fact which this parasite is common parasite of turtles in all over the world. T. assula might be transmitted between variety of reptiles so presence of the trematod should be considered as a risk factor for other reptiles.