RESUMEN
Intracellular pH (pHi) and several ion transport mechanisms in cultured murine salivary gland cells (SCA-9) were studied using a videomicrofluorometric method and the H+-specific probe C-SNARF-1. The aim of this study was to test SCA-9 cells' pHi regulation mechanisms and evaluate if this cell line is representative of submandibular gland cells. Resting pHi in unstimulated cells was estimated to be 7.17+/-0.07. To investigate the presence of Na+/H+ and Cl-/HCO3- antiports as well as Na+/K+/2Cl- symports in SCA-9 cells, we used different specific blockers, dimethyl-amiloride, disulfonic stilbene, bumetanide and furosemide. In order to study SCA-9 cell capacity to regulate their pHi in response to alkaline and acid loads, we applied the NH4Cl prepulse method to all these blockers. The results showed that SCA-9 cells possess both antiports and symports involved in pHi regulation, and that this cell line can be used as a convenient model to study pHi regulatory mechanisms in salivary cells.
Asunto(s)
Hidrógeno/metabolismo , Transporte Iónico , Glándula Submandibular/citología , Glándula Submandibular/metabolismo , Equilibrio Ácido-Base , Cloruro de Amonio , Animales , Antiportadores/antagonistas & inhibidores , Antiportadores/metabolismo , Transporte Biológico Activo , Proteínas Portadoras/antagonistas & inhibidores , Proteínas Portadoras/metabolismo , Antiportadores de Cloruro-Bicarbonato , Fluorometría , Concentración de Iones de Hidrógeno , Membranas Intracelulares/metabolismo , Ratones , Microscopía por Video , Modelos Biológicos , Intercambiadores de Sodio-Hidrógeno/antagonistas & inhibidores , Intercambiadores de Sodio-Hidrógeno/metabolismo , Simportadores de Cloruro de Sodio-Potasio , Células Tumorales CultivadasRESUMEN
pH and salivary lactobacilli were analysed in 22 healthy individuals and 34 patients with cancer tumors of the oral or oropharyngeal regions before and during irradiation therapy. The findings revealed in cancer patients before radiotherapy, that pH and lactobacilli were significantly lower than those in non cancer control subjects. During irradiation therapy, pH and lactobacilli were not significantly altered. No significant difference was found between patients cured by chemotherapy before radiotherapy and patients treated only by irradiations. However, pH is modified by teeth: toothless patients ph and toothed patients pH change in opposite direction.