RESUMEN
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RESUMEN
The mechanism of superconductivity in cuprates remains one of the big challenges of condensed matter physics. High-T c cuprates crystallize into a layered perovskite structure featuring copper oxygen octahedral coordination. Due to the Jahn Teller effect in combination with the strong static Coulomb interaction, the octahedra in high-T c cuprates are elongated along the c axis, leading to a 3dx 2-y 2 orbital at the top of the band structure wherein the doped holes reside. This scenario gives rise to 2D characteristics in high-T c cuprates that favor d-wave pairing symmetry. Here, we report superconductivity in a cuprate Ba2CuO4-y , wherein the local octahedron is in a very exceptional compressed version. The Ba2CuO4-y compound was synthesized at high pressure at high temperatures and shows bulk superconductivity with critical temperature (T c ) above 70 K at ambient conditions. This superconducting transition temperature is more than 30 K higher than the T c for the isostructural counterparts based on classical La2CuO4 X-ray absorption measurements indicate the heavily doped nature of the Ba2CuO4-y superconductor. In compressed octahedron, the 3d3z 2-r 2 orbital will be lifted above the 3dx 2-y 2 orbital, leading to significant 3D nature in addition to the conventional 3dx 2-y 2 orbital. This work sheds important light on advancing our comprehensive understanding of the superconducting mechanism of high T c in cuprate materials.
RESUMEN
We present a detailed study of the photoinduced magnetization precession and magnetic relaxation in epitaxial Pd/Fe films on MgO(0 0 1) substrates via all-optical pump-probe techniques. We explicitly formulate the correlation between the Gilbert damping and the effective damping in the optical approach. Furthermore, a non-local Gilbert damping induced by spin pumping is demonstrated self-consistently by the Gilbert damping dependence on Pd thickness and the [Formula: see text] relationship of damping with Fe thickness [Formula: see text]. The non-local Gilbert damping enables the determination of spin diffusion length [Formula: see text] in Pd and interfacial spin mixing conductance [Formula: see text]. Our work paves the way toward the optical determination of spin transport parameters.
RESUMEN
Recently a new diluted magnetic semiconductor, (Ba,K)(Zn,Mn)2As2 (BZA), with high Curie temperature was discovered, showing an independent spin and charge-doping mechanism. This makes BZA a promising material for spintronics devices. We report the successful growth of a BZA single crystal for the first time in this study. An Andreev reflection junction, which can be used to evaluate spin polarization, was fabricated based on the BZA single crystal. A 66% spin polarization of the BZA single crystal was obtained by Andreev reflection spectroscopy analysis.
RESUMEN
In cells of the mould Trichoderma viride, the existence of an antifungal protein (AFP)-like gene consisting of 285 bp was confirmed by Southern analysis that genomic DNA of T. viride could hybridize with the cDNA of mature AFP of Aspergillus giganteus MDH 18894. Except for the absence of two introns, the nucleotide sequence of the AFP-like gene was identical to that of the AFP gene of A. giganteus in positions 336-479, 568-649, and 706-765. The AFP-like gene could not be transcribed into its mRNA in T. viride cells as examined by RT-PCR using total RNAs of T. viride as template. Furthermore, AFP could not be detected either directly from the culture medium of T. viride or by Western analysis. However, the AFP-like gene could be actively expressed like the cDNA of AFP in Escherichia coli cell. Recombinant AFP exhibited similar antifungal activity as native AFP.
Asunto(s)
Genes Fúngicos , Trichoderma/genética , Secuencia de Aminoácidos , Aspergillus/genética , Secuencia de Bases , ADN Complementario/biosíntesis , ADN Complementario/aislamiento & purificación , Electroforesis en Gel de Poliacrilamida , Escherichia coli/genética , Proteínas Fúngicas/análisis , Proteínas Fúngicas/genética , Silenciador del Gen , Vectores Genéticos , Intrones , Datos de Secuencia Molecular , Proteínas Recombinantes/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Transcripción GenéticaRESUMEN
The coordinate expression of anthocyanin biosynthetic genes in leaves and stems of a red forma of Perilla frutescens is presumably controlled by regulatory gene(s). A Myc-like gene (Myc-rp) was isolated from a cDNA library prepared from the leaves of red P. frutescens, and its deduced amino acid sequence shows 64% identity with that of delila from snapdragon. The Myc-rp gene was expressed in leaves and roots of both red and green P. frutescens equally. Comparison of deduced amino acid sequence of Myc-rp with that of Myc-gp, the second allele isolated from a green forma of P. frutescens, indicates that the 132nd amino acid, alanine, existing in MYC-RP was changed to serine in MYC-GP. The heterologous expression of these two alleles of Myc-like gene in tobacco and tomato resulted in an increase of the anthocyanin contents in flowers of tobacco and vegetative tissues and flowers of tomato. However, the flowers of transgenic tobacco expressing the fragment with a partial deletion (encoding 1-115 amino acids deleted) of Myc(-gp gave no change in anthocyanin accumulation, but some morphological changes of the flower were observed. In yeast, the MYC-RP/GP and Delila protein exhibited transactivation activity on the GAL-1 promoter from yeast and the promoter of dihydroflavonol 4-reductase (DFR) gene from P. frutescens. A transactivation domain of MYC-RP/GP and Delila could be located in the region between the 193rd and the 420th amino acid of MYC-RP/GP proteins. Our data indicate that this Myc-like gene presumably functions in the regulation of anthocyanin biosynthesis similarly in different tissues of dicot plants.
Asunto(s)
Antocianinas/biosíntesis , Genes de Plantas/genética , Lamiaceae/genética , Proteínas de Plantas/genética , Oxidorreductasas de Alcohol/genética , Oxidorreductasas de Alcohol/metabolismo , Alelos , Secuencia de Aminoácidos , Secuencia de Bases , Northern Blotting , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , Regulación de la Expresión Génica de las Plantas/efectos de la radiación , Genes myc/genética , Humanos , Recién Nacido , Lamiaceae/química , Lamiaceae/metabolismo , Solanum lycopersicum/genética , Datos de Secuencia Molecular , Fenotipo , Pigmentación/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente/genética , Plantas Tóxicas , Regiones Promotoras Genéticas , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN Mensajero/efectos de la radiación , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Saccharomyces cerevisiae/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Distribución Tisular , Nicotiana/genética , Activación Transcripcional , beta-Galactosidasa/genética , beta-Galactosidasa/metabolismoRESUMEN
The Myb-p1 gene was isolated by screening for differentially expressed Myb-related genes in red (anthocyanin-producing) and green (anthocyanin nonproducing) forms of Perilla frutescens. Expression of Myb-p1 is increased 10-fold in the red relative to the green form of P. futescens, and the gene is induced by light. MYB-P1 has only one DNA-binding region, which corresponds to repeat III in the general structure of MYB proteins. In the yeast two-hybrid system, it was shown that MYB-P1 interacted with MYC-RP, a MYC-related transcriptional regulatory protein involved in the control of anthocyanin biosynthesis in P. frutescens. In yeast, MYB-P1 was able to bind to a dihydroflavonol reductase (DFR) gene promoter isolated from red P. frutescens. These data suggest that Myb-p1 may be involved in the regulation of anthocyanin biosynthesis and could therefore be responsible for determining anthocyanin formation in red P. frutescens.
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Antocianinas/biosíntesis , Proteínas de Unión al ADN/biosíntesis , Regulación de la Expresión Génica de las Plantas , Lamiaceae/efectos de la radiación , Oncogenes , Proteínas de Plantas/biosíntesis , Plantas Medicinales/efectos de la radiación , Proteínas Proto-Oncogénicas c-myb , Oxidorreductasas de Alcohol/genética , Secuencia de Aminoácidos , Secuencia de Bases , Color , Genes de Plantas , Genes myc , Luz , Datos de Secuencia Molecular , Regiones Promotoras Genéticas , Unión Proteica , Homología de Secuencia de Aminoácido , Factores de Transcripción/metabolismoRESUMEN
A simple method for preparation of alpha-sarcin and an antifungal protein (AFP) from the mold Aspergillus giganteus MDH 18894 has been developed. alpha-Sarcin and AFP were purified simultaneously by carboxymethylcellulose 52 cation-exchange chromatography and Blue Sepharose CL-6B affinity chromatography. By this method, 4.2 mg of alpha-sarcin and 6.8 mg of AFP were obtained from 2 liters of medium. Compared with other purification methods such as gel-filtration chromatography, this procedure was simple and specific. The purified alpha-sarcin and AFP were homogeneous characterized on SDS-polyacrylamide gel. The enzymatic activity of several ribosome-inactivating proteins such as alpha-sarcin, trichosanthin, and cinnamomin was significantly inhibited by the dye Cibacron blue F3GA. In 50 microliter of reaction mixture, 10 microM of the dye could inhibit 50% activity of cinnamomin (7 x 10(-9) M), whereas 50% inhibition of the enzymatic activity of trichosanthin (7 x 10(-9) M) and alpha-sarcin (1 x 10(-7) M) required 100 and 50 microM of the dye, respectively.
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Antifúngicos/química , Aspergillus/química , Endorribonucleasas/química , Proteínas Algáceas , División Celular/efectos de los fármacos , Cromatografía de Afinidad/métodos , Cromatografía por Intercambio Iónico/métodos , Inhibidores Enzimáticos/farmacología , Proteínas Fúngicas/aislamiento & purificación , Proteínas/metabolismo , Ribonucleasas/química , Proteínas Inactivadoras de Ribosomas Tipo 2 , Ribosomas/metabolismo , Sefarosa/análogos & derivados , Sefarosa/metabolismo , Triazinas/farmacología , Tricosantina/metabolismo , Verticillium/efectos de los fármacosRESUMEN
Molecular analysis of the t(10;14) chromosomal translocation found in pediatric patients with T-cell acute lymphoblastic leukemia has led to the identification of the HOX-11 (TCL-3) protooncogene. The HOX-11 cDNA contains an open reading frame encoding a homeoprotein with features of DNA-binding. The majority of the t(10;14) chromosomal translocation breakpoints have been mapped to the 5' end of the HOX-11 gene, supporting the notion that deregulation of the HOX-11 gene by the t(10;14) chromosomal translocation contributed importantly to leukemia formation. To further define the role of the HOX-11 homeoprotein, we have prepared rabbit antiserum against a trpE-HOX-11 fusion protein. The purified anti-HOX-11 IgG immuno-precipitated a protein with apparent relative molecular mass of 40 kD. Biochemical fractionation demonstrated that the protein is localized in the nucleus. Furthermore, the HOX-11 RNA and protein appeared to be modulated during the cell cycle, with the highest level of expression at G1/S phase boundary. Taken together, these data suggest that the HOX-11 gene product may function as a transcription factor for G1 progression in the cell cycle.
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Ciclo Celular/fisiología , Regulación de la Expresión Génica/genética , Genes Homeobox/genética , Proteínas de Homeodominio , Proteínas Nucleares/genética , Proto-Oncogenes/genética , Secuencia de Aminoácidos , Secuencia de Bases , Western Blotting , Núcleo Celular/química , ADN de Neoplasias/análisis , ADN de Neoplasias/genética , Proteínas de Unión al ADN/análisis , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/fisiología , Electroforesis en Gel de Poliacrilamida , Fase G1 , Regulación de la Expresión Génica/fisiología , Humanos , Sueros Inmunes , Leucemia-Linfoma de Células T del Adulto/genética , Leucemia-Linfoma de Células T del Adulto/metabolismo , Leucemia-Linfoma de Células T del Adulto/patología , Datos de Secuencia Molecular , Proteínas Nucleares/metabolismo , Proteínas Oncogénicas/análisis , Proteínas Oncogénicas/genética , Proteínas Oncogénicas/fisiología , Pruebas de Precipitina , Proto-Oncogenes Mas , Proteínas Proto-Oncogénicas , ARN Neoplásico/análisis , ARN Neoplásico/genética , Fase S , Factores de Transcripción/fisiología , Translocación Genética/genéticaRESUMEN
Arrowhead double-headed proteinase inhibitors A and B consist of 150 amino acid residues with three disulfide bonds. Based on their primary structures, three cDNA fragments of the inhibitors were amplified in vitro by the PCR method using a constructed arrowhead cDNA library as a template. With the overlapping sequences, the full-length cDNA sequences of the inhibitors were then ascertained. The open reading frame encodes a pre-inhibitor, including a 24 residue signal peptide. The deduced amino acid sequences are consistent in principle with those determined by primary structure analysis, except that there are seven extra residues in the C-terminal part of the inhibitors, which might be cleaved off by proteinase post-processing immediately after protein synthesis. It is worth pointing out that cDNAs of both inhibitors A and B contain an 87 bp intron in the AAG codon of residue Lys-97. According to the elucidated cDNA sequences, the structural genes of inhibitors A and B were amplified using the total cDNA or genomic DNA of arrowhead as a PCR template. It was indicated that both the cDNA and genomic structures of inhibitors A and B have the same sequences.
Asunto(s)
Proteínas de Plantas/genética , Inhibidores de Proteasas , Secuencia de Aminoácidos , Secuencia de Bases , Sitios de Unión , ADN , Biblioteca de Genes , Genes de Plantas , Datos de Secuencia Molecular , Proteínas de Plantas/metabolismo , Plantas/genética , Plantas/metabolismo , Reacción en Cadena de la PolimerasaRESUMEN
The glutathione S-transferases (EC 2.5.1.18) have been purified to electrophoretic homogeneity from 105,000g supernatant of sheep liver homogenate by employing a combination of gel filtration on Sephadex G-150 and affinity chromatography on S-hexylglutathione-linked Sepharose-6B columns. Approximately 70% of the original glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene and glutathione peroxidase activity toward cumene hydroperoxide could be recovered by this purification method. Of particular importance in developing this procedure was the fact that the enzyme preparation obtained after affinity column chromatography represented all the isozymes of sheep liver glutathione S-transferases. Further purification by CM-cellulose and DEAE-cellulose column chromatography resolved the glutathione S-transferases into seven distinct cationic isozymes designated C-1, C-2, C-3, C-4, C-5, C-6, and C-7 and five overlapping anionic transferases designated A-1, A-2, A-3, A-4, and A-5, respectively, in the order of their elution from the ion-exchange columns. The sodium dodecyl sulfate SDS-gel electrophoretic data on subunit composition revealed that cationic enzymes are composed of two subunits with an identical Mr of 24,000 whereas a predominant subunit with Mr of 26,000 was observed in all anionic isozyme peaks except A-1. Cationic isozymes accounted for approximately 98% of the total peroxidase activity associated with the glutathione S-transferase whereas only A-1 of the anionic isozymes displayed some peroxidase activity. Isozyme C-4 was found to be the most abundant glutathione S-transferase in the sheep liver. Characterization of the individual transferases by their specificity toward a number of selected substrates, subunit composition, and isoelectric points showed some similarities to those patterns for human liver glutathione S-transferases.