RESUMEN
Late blight disease caused by the plant pathogenic oomycete pathogen Phytophthora infestans is one of the most limiting factors in potato production. P. infestans is able to overcome introgressed late blight resistance by adaptation of effector genes. AVR1 is an RXLR effector that triggers immune responses when recognized by the potato resistance protein R1. P. infestans isolates avirulent on R1 plants were found to have AVR1 variants that are recognized by R1. Virulent isolates though, lack AVR1 but do contain a close homologue of AVR1, named A-L, of which all variants escape recognition by R1. Co-expression of AVR1 and R1 in Nicotiana benthamiana results in a hypersensitive response (HR). In contrast, HR is not activated when A-L is co-expressed with R1. AVR1 and A-L are highly similar in structure. They share two W motifs and one Y motif in the C-terminal part but differ in the T-region, a 38 amino acid extension at the carboxyl-terminal tail of AVR1 lacking in A-L. To pinpoint what determines R1-mediated recognition of AVR1 we tested elicitor activity of AVR1 and A-L chimeric and deletion constructs by co-expression with R1. The T-region is important as it enables R1-mediated recognition of A-L, not only when fused to A-L but also via trans-complementation. Yet, AVR1 lacking the T-region is still active as an elicitor of HR, but this activity is lost when certain motifs are swapped with A-L. These data show that A-L circumvents R1 recognition not only because it lacks the T-region, but also because of differences in the conserved C-terminal effector motifs.
RESUMEN
G-protein-coupled receptors (GPCRs) are key cellular components that mediate extracellular signals into intracellular responses. Genome mining revealed that Phytophthora spp. have over 60 GPCR genes among which a prominent class of 12 encoding novel proteins with an N-terminal GPCR domain fused to a C-terminal phosphatidylinositol phosphate kinase (PIPK) domain. This study focuses on two GPCR-PIPKs (GKs) in Phytophthora sojae. PsGK4 and PsGK5 are differentially expressed during the life cycle with the highest expression in cysts and during cyst germination, and at late infection stages. In P. sojae transformants that constitutively express RFP-tagged PsGK4 and PsGK5, the fusion proteins in hyphae reside in small, rapidly moving vesicular-like structures. Functional analysis using gene silencing showed that PsGK4-silenced transformants displayed higher levels of encystment and a reduced cyst germination rate when compared with the recipient strain. Moreover, GK4 deficiency (or reduction) resulted in severe defects in zoospore chemotaxis towards isoflavones and soybean roots. In contrast, PsGK5-silenced transformants exhibited no obvious defects in asexual development but oospore production was severely impaired. Both, PsGK4- and PsGK5-silenced transformants showed reduced pathogenicity. These results point to involvement of GKs in zoospore behaviour, chemotaxis and oospore development, and suggest that PsGK4 and PsGK5 each head independent signalling pathways.
Asunto(s)
Quimiotaxis , Regulación del Desarrollo de la Expresión Génica , Fosfatos de Fosfatidilinositol/metabolismo , Fosfotransferasas/metabolismo , Phytophthora/crecimiento & desarrollo , Phytophthora/fisiología , Receptores Acoplados a Proteínas G/química , Esporas/fisiología , Proteínas Algáceas/genética , Proteínas Algáceas/metabolismo , Isoflavonas , Fosfotransferasas/química , Fosfotransferasas/genética , Phytophthora/genética , Phytophthora/metabolismo , Enfermedades de las Plantas/microbiología , Raíces de Plantas/microbiología , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Transducción de Señal , Glycine max/microbiologíaRESUMEN
For a comprehensive survey of the structure and dynamics of the Dutch Phytophthora infestans population, 652 P. infestans isolates were collected from commercial potato fields in the Netherlands during the 10-year period 2000-2009. Genotyping was performed using 12 highly informative microsatellite markers and mitochondrial haplotypes. In addition, for each isolate, the mating type was determined. STRUCTURE analysis grouped the 322 identified genotypes in three clusters. Cluster 1 consists of a single clonal lineage NL-001, known as "Blue_13"; all isolates in this cluster have the A2 mating type and the Ia mitochondrial haplotype. Clusters 2 and 3 display a more elaborate substructure containing many unique genotypes. In Cluster 3, several distinct clonal lineages were also identified. This survey witnesses that the Dutch population underwent dramatic changes in the 10 years under study. The most notable change was the emergence and spread of A2 mating type strain NL-001 (or "Blue_13"). The results emphasize the importance of the sexual cycle in generating genetic diversity and the importance of the asexual cycle as the propagation and dispersal mechanism for successful genotypes. Isolates were also screened for absence of the Avrblb1/ipiO class I gene, which is indicative for virulence on Rpi-blb1. This is also the first report of Rpi-blb1 breakers in the Netherlands. Superimposing the virulence screening on the SSR genetic backbone indicates that lack the Avrblb1/ipiO class I gene only occurred in sexual progeny. So far, the asexual spread of the virulent isolates identified has been limited.
Asunto(s)
Ligamiento Genético , Phytophthora infestans/genética , Análisis por Conglomerados , ADN Mitocondrial/genética , Genotipo , Haplotipos , Repeticiones de Microsatélite , Países Bajos , Phytophthora infestans/patogenicidad , Polimorfismo Genético , Dinámica Poblacional , Solanum tuberosum/parasitología , Virulencia/genéticaRESUMEN
This article documents the addition of 396 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Anthocidaris crassispina, Aphis glycines, Argyrosomus regius, Astrocaryum sciophilum, Dasypus novemcinctus, Delomys sublineatus, Dermatemys mawii, Fundulus heteroclitus, Homalaspis plana, Jumellea rossii, Khaya senegalensis, Mugil cephalus, Neoceratitis cyanescens, Phalacrocorax aristotelis, Phytophthora infestans, Piper cordulatum, Pterocarpus indicus, Rana dalmatina, Rosa pulverulenta, Saxifraga oppositifolia, Scomber colias, Semecarpus kathalekanensis, Stichopus monotuberculatus, Striga hermonthica, Tarentola boettgeri and Thermophis baileyi. These loci were cross-tested on the following species: Aphis gossypii, Sooretamys angouya, Euryoryzomys russatus, Fundulus notatus, Fundulus olivaceus, Fundulus catenatus, Fundulus majalis, Jumellea fragrans, Jumellea triquetra Jumellea recta, Jumellea stenophylla, Liza richardsonii, Piper marginatum, Piper aequale, Piper darienensis, Piper dilatatum, Rana temporaria, Rana iberica, Rana pyrenaica, Semecarpus anacardium, Semecarpus auriculata, Semecarpus travancorica, Spondias acuminata, Holigarna grahamii, Holigarna beddomii, Mangifera indica, Anacardium occidentale, Tarentola delalandii, Tarentola caboverdianus and Thermophis zhaoermii.
RESUMEN
Phenotypic and genotypic characteristics of 48 Phytophthora infestans isolates, collected in five provinces in Northern China between 1997 and 2003, were determined and compared with reference isolates. Characterisation included mating type, virulence, mitochondrial DNA (mtDNA) haplotype and DNA fingerprinting patterns based on simple sequence repeats (SSR) and amplified fragment length polymorphisms (AFLP). All isolates had the A1 mating type, mtDNA haplotype IIa and an identical SSR genotype (designated as SG-01-01) that differed from SSR genotypes found in the reference isolates, including those representing the 'old' US-1 lineage that dominated the P. infestans population worldwide prior to 1980. In contrast, the virulence spectra were highly variable and virulence to all resistance genes present in the standard differential set (R1 to R11) was found. AFLP analysis revealed some diversity; eight different AFLP genotypes were found that could be grouped into two major clusters. This study shows that there is very little genotypic diversity in the P. infestans population in Northern China. The occurrence of many different races within this rather uniform population is discussed in the framework of recent insights into the molecular determinants of avirulence in potato-P. infestans'gene-for-gene' interactions.
Asunto(s)
Variación Genética , Phytophthora infestans/genética , Phytophthora infestans/patogenicidad , Enfermedades de las Plantas/microbiología , Solanum tuberosum/microbiología , Análisis del Polimorfismo de Longitud de Fragmentos Amplificados/métodos , China , ADN Mitocondrial/genética , Genotipo , Haplotipos , Repeticiones de Minisatélite , Fenotipo , Dinámica Poblacional , Virulencia/genéticaRESUMEN
The RGD tripeptide sequence, a cell adhesion motif present in several extracellular matrix proteins of mammalians, is involved in numerous plant processes. In plant-pathogen interactions, the RGD motif is believed to reduce plant defence responses by disrupting adhesions between the cell wall and plasma membrane. Photoaffinity cross-linking of [125I]-azido-RGD heptapeptide in the presence of purified plasma membrane vesicles of Arabidopsis thaliana led to label incorporation into a single protein with an apparent molecular mass of 80 kDa. Incorporation could be prevented by excess RGD peptides, but also by the IPI-O protein, an RGD-containing protein secreted by the oomycete plant pathogen Phytophthora infestans. Hydrophobic cluster analysis revealed that the RGD motif of IPI-O (positions 53-56) is readily accessible for interactions. Single amino acid mutations in the RGD motif in IPI-O (of Asp56 into Glu or Ala) resulted in the loss of protection of the 80-kDa protein from labelling. Thus, the interaction between the two proteins is mediated through RGD recognition and the 80-kDa RGD-binding protein has the characteristics of a receptor for IPI-O. The IPI-O protein also disrupted cell wall-plasma membrane adhesions in plasmolysed A. thaliana cells, whereas IPI-O proteins mutated in the RGD motif (D56A and D56E) did not.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Proteínas Fúngicas/metabolismo , Phytophthora/metabolismo , Secuencias de Aminoácidos , Membrana Celular/metabolismo , Pared Celular/metabolismo , Proteínas de la Membrana/metabolismo , Oligopéptidos/metabolismoRESUMEN
The oomycete plant pathogen Phytophthora infestans is the causal agent of late blight, one of the most devastating diseases of potato worldwide. As part of efforts to clone avirulence (Avr) genes and pathogenicity factors from P. infestans, we have constructed a bacterial artificial chromosome (BAC) library from an isolate containing six Avr genes. The BAC library comprises clones with an average insert size of 98 kb and represents an estimated 10 genome equivalents. A three-dimensional pooling strategy was developed to screen the BAC library for amplified fragment length polymorphism (AFLP) markers, as this type of marker has been extensively used in construction of a P. infestans genetic map. Multiple positive clones were identified for each AFLP marker tested. The pools were used to construct a contig of 11 BAC clones in a region of the P. infestans genome containing a cluster of three avirulence genes. The BAC contig is predicted to encompass the Avr11 locus but mapping of the BAC ends will be required to determine if the Avr3 and Avr10 loci are also present in the BAC contig. These results are an important step towards the positional cloning of avirulence genes from P. infestans, and the BAC library represents a valuable resource for largescale studies of oomycete genome organisation and gene content.
Asunto(s)
ADN/genética , Phytophthora/genética , Cromosomas Artificiales Bacterianos/genética , Clonación Molecular , Mapeo Contig , Biblioteca Genómica , Hibridación de Ácido Nucleico , Mapeo Físico de Cromosoma , Phytophthora/patogenicidad , Virulencia/genéticaRESUMEN
The oomycete genus Phytophthora contains some of the world's most devastating plant pathogens. We report here the existence in P. cinnamomi of four genes encoding the pyrophosphate-utilizing glycolytic/gluconeogenic enzyme pyruvate, phosphate dikinase (PPDK). The coding regions of the four genes are >99% identical. At least three of the genes comprise a small gene cluster, which may have arisen through recent gene duplication and inversion events. Levels of Pdk mRNA are low in vegetative hyphae, but increase rapidly and transiently upon transfer of cultures to nutrient-free media, conditions that trigger asexual sporulation. PPDK protein and enzyme activity levels do not show a similar increase during sporulation. Assays of PPDK activity in P. cinnamomi hyphal extracts suggest that the majority of glycolytic flux in sporulating hyphae probably occurs via PPDK, rather than pyruvate kinase. This finding, combined with the existence of Phytophthora-expressed sequence tags encoding two other pyrophosphate-utilizing enzymes, indicates that pyrophosphate-based metabolism may be important in Phytophthora. The possibility that PPDK and other enzymes of pyrophosphate-based metabolism may provide targets for the development of novel control measures for Phytophthora and other oomycete pathogens is discussed.
Asunto(s)
Familia de Multigenes , Phytophthora/enzimología , Phytophthora/genética , Piruvato Ortofosfato Diquinasa/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , ADN Complementario/genética , Expresión Génica , Datos de Secuencia Molecular , Phytophthora/crecimiento & desarrollo , Phytophthora/patogenicidad , Plantas/microbiología , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Esporas/enzimologíaRESUMEN
Recent phylogenetic analyses of the nucleotide binding sites (NBS)-leucine-rich repeats (LRR) class of plant disease resistance (R) genes suggest that these genes are ancient and coexist next to susceptibility alleles at resistance loci. Another class of R genes encodes serine-threonine protein kinases related to Pto that were originally identified from wild relatives of tomato. In this study, we exploit the highly diverse genus Solanum to identify Pto-like sequences and test various evolutionary scenarios for Pto-like genes. Polymerase chain reaction amplifications with the use of primers that were developed on the basis of conserved and variable regions of Pto revealed an extensive Pto gene family and yielded 32 intact Pto-like sequences from six Solanum species. Furthermore, Pto-like transcripts were detected in the leaf tissue of all tested plants. The kinase consensus and autophosphorylation sites were highly conserved, in contrast to the kinase activation domain, which is involved in ligand recognition in Pto. Phylogenetic analyses distinguished nine classes of Pto-like genes and revealed that orthologs were more similar than paralogs, suggesting that the Pto gene family evolved through a series of ancient gene duplication events prior to speciation in Solanum. Thus, like the NBS-LRR class, the kinase class of R genes is highly diverse and ancient.
Asunto(s)
Evolución Biológica , Genes de Plantas , Familia de Multigenes , Proteínas de Plantas , Proteínas Serina-Treonina Quinasas/genética , Solanaceae/genética , Secuencia de Aminoácidos , Dominio Catalítico/genética , Secuencia Conservada , Cartilla de ADN , Modelos Genéticos , Datos de Secuencia Molecular , Filogenia , Enfermedades de las Plantas/genética , Hojas de la Planta/genética , Solanaceae/clasificación , Solanaceae/enzimología , Especificidad de la EspecieRESUMEN
In this study we investigated the genetic control of avirulence in the diploid oomycete pathogen Phytophthora infestans, the causal agent of late blight on potato. The dominant avirulence (Avr) genes matched six race-specific resistance genes introgressed in potato from a wild Solanum species. AFLP markers linked to Avr genes were selected by bulked segregant analysis and used to construct two high-density linkage maps, one containing Avr4 (located on linkage group A2-a) and the other containing a cluster of three tightly linked genes, Avr3, Avr10, and Avr11 (located on linkage group VIII). Bulked segregant analysis also resulted in a marker linked to Avr1 and this allowed positioning of Avr1 on linkage group IV. No bulked segregant analysis was performed for Avr2, but linkage to a set of random markers placed Avr2 on linkage group VI. Of the six Avr genes, five were located on the most distal part of the linkage group, possibly close to the telomere. The high-density mapping was initiated to facilitate future positional cloning of P. infestans Avr genes.
Asunto(s)
Phytophthora/genética , Polimorfismo Genético/genética , Virulencia/genética , Mapeo Cromosómico , Clonación Molecular , Dermatoglifia del ADN , Genes Dominantes , Ligamiento Genético , Marcadores Genéticos , Genotipo , Escala de Lod , Modelos Genéticos , FenotipoRESUMEN
In Phytophthora infestans, a cluster of three dominant avirulence genes is located on the distal part of linkage group VIII. In a mapping population from a cross between two Dutch field isolates, probe M5.1, derived from an amplified fragment length polymorphism (AFLP) marker linked to the Avr3-Avr10-Avr11 cluster, hybridized only to DNA from the parent and F1 progeny that is avirulent on potato lines carrying the R3, R10, and R11 resistance gene. In the virulent parent and the virulent progeny, no M5.1 homologue was detected, demonstrating a deletion on that part of linkage group VIII. P. infestans is diploid, so the avirulent strains must be hemizygous for the region concerned. A similar situation was found in another mapping population from two Mexican strains. The deletion was also found to occur in many field isolates. In a large set of unique isolates collected in The Netherlands from 1980 to 1991, 37% had no M5.1 homologue and the deletion correlated strongly with gain of virulence on potato lines carrying R3, R10, and R11. Also, in some old isolates that belong to a single clonal lineage (US-1) and are thus highly homogenous, deletions at the M5.1 locus were detected, indicating that this region is unstable.
Asunto(s)
Deleción Cromosómica , Phytophthora/genética , Phytophthora/patogenicidad , Solanum tuberosum/genética , Solanum tuberosum/microbiología , Southern Blotting , Cruzamientos Genéticos , Ligamiento Genético , Marcadores Genéticos , Genotipo , Familia de Multigenes , Polimorfismo Genético , Solanum tuberosum/clasificación , Virulencia/genéticaRESUMEN
We characterized pharmacologically the hypersensitive cell death of tobacco BY-2 cells that followed treatments with Escherichia coli preparations of INF1, the major secreted elicitin of the late blight pathogen Phytophthora infestans. INF1 elicitin treatments resulted in fragmentation and 180 bp laddering of tobacco DNA as early as 3 h post-treatment. INF1 elicitin also induced rapid accumulation of H2O2 typical of oxidative burst, and the expression of defense genes such as phenylalanine ammonia-lyase (PAL) gene at 1 h and 3 h after elicitin treatment, respectively. To investigate the involvement of the oxidative burst and/or the expression of defense genes in the signal transduction pathways leading to hypersensitive cell death, we analyzed the effect of several chemical inhibitors of signal transduction pathways on the various responses. The results indicated that (a) the cell death required serine proteases, Ca2+ and protein kinases, (b) the oxidative burst was involved in Ca2+ and protein kinase mediated pathways, but elicitin-induced AOS was neither necessary nor sufficient for cell death and PAL gene expression, and (c) the signaling pathway of PAL gene expression required protein kinases. These results suggest that the three signal transduction pathways leading to cell death, oxidative burst and expression of defense genes branch in the early stages that follow elicitin recognition by tobacco cells.
Asunto(s)
Apoptosis/fisiología , Proteínas Fúngicas/farmacología , Regulación de la Expresión Génica de las Plantas , Nicotiana/citología , Nicotiana/fisiología , Plantas Tóxicas , Estallido Respiratorio/fisiología , Proteínas Algáceas , Apoptosis/efectos de los fármacos , Señalización del Calcio/fisiología , Células Cultivadas , Fragmentación del ADN/efectos de los fármacos , Escherichia coli , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Cinética , Fenilanina Amoníaco-Liasa/genética , Phytophthora , Proteínas Quinasas/metabolismo , Proteínas , Especies Reactivas de Oxígeno/fisiología , Proteínas Recombinantes/farmacología , Estallido Respiratorio/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Nicotiana/genéticaRESUMEN
From a set of Phytophthora infestans cDNA clones randomly selected from a potato-P. infestans interaction cDNA library, three out of 22 appeared to correspond to a gene encoding translation elongation factor 1alpha. The gene, called tef1, is a single copy gene in P. infestans. During the life cycle of P. infestans, tef1 is expressed in all developmental stages. Alignment and phylogeny analysis based on EF-1alpha proteins from several taxonomic groups, including fungi, slime molds, algae, higher plants and archeabacteria, support the view that oomycetes evolved completely independently from the true fungi. In the phylogenetic tree, P. infestans EF-1alpha forms one branch with EF-1alpha from the unicellular alga Cyanophora paradoxa, an organism belonging to a taxonomic group that occupies a key position in the evolution of plastids.
Asunto(s)
Factor 1 de Elongación Peptídica/genética , Phytophthora/genética , Proteínas de Saccharomyces cerevisiae , Secuencia de Aminoácidos , Secuencia de Bases , Southern Blotting , ADN Complementario/química , ADN Complementario/genética , ADN de Hongos/genética , Regulación Fúngica de la Expresión Génica , Datos de Secuencia Molecular , Filogenia , ARN de Hongos/genética , ARN de Hongos/metabolismo , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
The interaction between Phytophthora infestans (Mont.) de Bary and Solanum was examined cytologically using a diverse set of wild Solanum species and potato (S. tuberosum L.) cultivars with various levels of resistance to late blight. In wild Solanum species, in potato cultivars carrying known resistance (R) genes and in nonhosts the major defense reaction appeared to be the hypersensitive response (HR). In fully resistant Solanum species and nonhosts, the HR was fast and occurred within 22 h. This resulted in the death of one to three cells. In partially resistant clones, the HR was induced between 16 and 46 h, and resulted in HR lesions consisting of five or more dead cells, from which hyphae were occasionally able to escape to establish a biotrophic interaction. These results demonstrate the quantitative nature of the resistance to P. infestans. The effectiveness of the HR in restricting growth of the pathogen differed considerably between clones and correlated with resistance levels. Other responses associated with the defense reaction were deposition of callose and extracellular globules containing phenolic compounds. These globules were deposited near cells showing the HR, and may function in cell wall strengthening.
Asunto(s)
Phytophthora/patogenicidad , Enfermedades de las Plantas/microbiología , Solanaceae/microbiología , Fenoles/metabolismo , Enfermedades de las Plantas/genética , Solanaceae/citología , Solanaceae/genéticaRESUMEN
From a set of Phytophthora infestans cDNA clones that were randomly selected from a potato- P. infestans interaction cDNA library, a relatively high proportion (5 out of 22) appeared to be derived from the same gene. The gene was designated ric1. P. infestans contains two copies of ric1 which share 98% homology at the nucleotide-sequence level and 100% at the amino-acid level. The nucleotide sequence predicts an open reading frame of 171 bp encoding a 57 amino-acid hydrophobic-peptide with two potential membrane-spanning domains. The predicted peptide shows high homology to a peptide encoded by plant genes whose expression is specifically induced during stress conditions. Southern-blot analysis of genomic DNA of several Phytophthora species indicated that most species contain ric1 homologues. During the life cycle of P. infestans, ric1 was expressed in all developmental stages but the level of expression varied. Sporangia and germinating cysts appeared to contain only very little ric1 mRNA whereas in the mycelium and during in planta growth higher levels were detected. Subjecting the mycelium to osmotic stress or to a high pH resulted in increased ric1 expression.
Asunto(s)
Genes de Plantas , Phytophthora/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , ADN Complementario/genética , ADN de Plantas/genética , Expresión Génica , Datos de Secuencia Molecular , Proteínas de Unión al GTP Monoméricas/genética , Phytophthora/patogenicidad , Proteínas de Plantas/genética , Solanum tuberosum/microbiologíaRESUMEN
A total of 1000 expressed sequence tags (ESTs) corresponding to 760 unique sequence sets were identified using random sequencing of clones from a cDNA library constructed from mycelial RNA of Phytophthora infestans. A number of software programs, represented by a relational database and an analysis pipeline, were developed for the automated analysis and storage of the EST sequence data. A set of 419 nonredundant sequences, which correspond to a total of 632 ESTs (63.2%), were identified as showing significant matches to sequences deposited in public databases. A putative cellular identity and role was assigned to all 419 sequences. All major functional categories were represented by at least several ESTs. Four novel cDNAs containing sequences related to elicitins, a family of structurally related proteins that induce the hypersensitive response and condition avirulence of P. infestans on Nicotiana plants, were among the most notable genes identified. Two of these elicitin-like cDNAs were among the most abundant cDNAs examined. The set also contained several ESTs with high sequence similarity to unique plant genes.
Asunto(s)
Etiquetas de Secuencia Expresada , Proteínas Fúngicas/metabolismo , Variación Genética , Phytophthora/genética , Actinas/genética , Actinas/metabolismo , Proteínas Algáceas/genética , Algoritmos , ADN Complementario/genética , Proteínas Fúngicas/genética , Biblioteca de Genes , Filogenia , Phytophthora/clasificación , Phytophthora/crecimiento & desarrollo , Plantas Tóxicas , Proteínas , Análisis de Secuencia de ADN , Programas Informáticos , Nicotiana/microbiologíaRESUMEN
Transformation of the diploid oomycete plant pathogen Phytophthora infestans with antisense, sense, and promoter-less constructs of the coding sequence of the elicitin gene inf1 resulted in transcriptional silencing of both the transgenes and the endogenous gene. Since heterokaryons obtained by somatic fusion of an inf1-silenced transgenic strain and a wild-type strain displayed stable gene silencing, inf1 silencing is dominant and acts in trans. Inf1 remained silenced in nontransgenic homokaryotic progeny from the silenced heterokaryons, thereby demonstrating that the presence of transgenes is not essential for maintaining the silenced status of the endogenous inf1 gene. These findings support a model reminiscent of paramutation and involving a trans-acting factor that is capable of transferring a silencing signal between nuclei.
Asunto(s)
Proteínas Algáceas , Núcleo Celular/genética , Regulación de la Expresión Génica , Phytophthora/genética , Antibacterianos/farmacología , Elementos sin Sentido (Genética)/genética , Southern Blotting , Fusión Celular/genética , Núcleo Celular/metabolismo , Medios de Cultivo Condicionados , Metilación de ADN , Análisis Mutacional de ADN , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Dosificación de Gen , Phytophthora/efectos de los fármacos , Phytophthora/crecimiento & desarrollo , Phytophthora/metabolismo , Plantas/microbiología , Regiones Promotoras Genéticas/genética , Proteínas , ARN Mensajero/biosíntesis , ARN Mensajero/metabolismo , Eliminación de Secuencia/genética , Transactivadores/metabolismo , Transcripción Genética/genética , Transformación Genética , Transgenes/genéticaRESUMEN
Phytophthora infestans, the agent of potato and tomato late blight disease, produces a 10-kD extracellular protein, INF1 elicitin. INF1 induces a hypersensitive response in a restricted number of plants, particularly those of the genus Nicotiana. In virulence assays with different P. infestans isolates, five Nicotiana species displayed resistance responses. In all of the interactions, after inoculation with P. infestans zoospores, penetration of an epidermal cell was observed, followed by localized necrosis typical of a hypersensitive response. To determine whether INF1 functions as an avirulence factor in these interactions, we adopted a gene-silencing strategy to inhibit INF1 production. Several transformants deficient in inf1 mRNA and INF1 protein were obtained. These strains remained pathogenic on host plants. However, in contrast to the wild-type and control transformant strains, INF1-deficient strains induced disease lesions when inoculated on N. benthamiana. These results demonstrate that the elicitin INF1 functions as an avirulence factor in the interaction between N. benthamiana and P. infestans.