Recruitment of a threatened foundation oyster species varies with large and small spatial scales.

Understanding how habitat attributes (e.g., patch area and sizes, connectivity) control recruitment and how this is modified by processes operating at larger spatial scales is fundamental to understanding population sustainability and developing successful long-term restoration strategies for marine foundation species-including for globally threatened reef-forming oysters. In two experiments, we assessed the recruitment and energy reserves of oyster recruits onto remnant reefs of the oyster Saccostrea glomerata in estuaries spanning 550 km of coastline in southeastern Australia. In the first experiment, we determined whether recruitment of oysters to settlement plates in three estuaries was correlated with reef attributes within patches (distances to patch edges and surface elevation), whole-patch attributes (shape and size of patches), and landscape attributes (connectivity). We also determined whether environmental factors (e.g., sedimentation and water temperature) explained the differences among recruitment plates. We also tested whether differences in energy reserves of recruits could explain the differences between two of the estuaries (one high- and one low-sedimentation estuary). In the second experiment, across six estuaries (three with nominally high and three with nominally low sedimentation rates), we tested the hypothesis that, at the estuary scale, recruitment and survival were negatively correlated to sedimentation. Overall, total oyster recruitment varied mostly at the scale of estuaries rather than with reef attributes and was negatively correlated with sedimentation. Percentage recruit survival was, however, similar among estuaries, although energy reserves and condition of recruits were lower at a high- compared to a low-sediment estuary. Within each estuary, total oyster recruitment increased with patch area and decreased with increasing tidal height. Our results showed that differences among estuaries have the largest influence on oyster recruitment and recruit health and this may be explained by environmental processes operating at the same scale. While survival was high across all estuaries, growth and reproduction of oysters on remnant reefs may be affected by sublethal effects on the health of recruits in high-sediment estuaries. Thus, restoration programs should consider lethal and sublethal effects of whole-estuary environmental processes when selecting sites and include environmental mitigation actions to maximize recruitment success.

Habitat traits and predation interact to drive abundance and body size patterns in associated fauna.

Habitat-forming organisms provide three-dimensional structure that supports abundant and diverse communities. Variation in the morphological traits of habitat formers will therefore likely influence how they facilitate associated communities, either via food and habitat provisioning, or by altering predator-prey interactions. These mechanisms, however, are typically studied in isolation, and thus, we know little of how they interact to affect associated communities. In response to this, we used naturally occurring morphological variability in the alga Sargassum vestitum to create habitat units of distinct morphotypes to test whether variation in the morphological traits (frond size and thallus size) of S. vestitum or the interaction between these traits affects their value as habitat for associated communities in the presence and absence of predation. We found morphological traits did not interact, instead having independent effects on epifauna that were negligible in the absence of predation. However, when predators were present, habitat units with large fronds were found to host significantly lower epifaunal abundances than other morphotypes, suggesting that large frond alga provided low-value refuge from predators. The presence of predators also influenced the size structure of epifaunal communities from habitat units of differing frond size, suggesting that the refuge value of S. vestitum was also related to epifauna body size. This suggests that habitat formers may chiefly structure associated communities by mediating size-selective predation, and not through habitat provisioning. Furthermore, these results also highlight that habitat traits cannot be considered in isolation, for their interaction with biotic processes can have significant implications for associated communities.

Organic enrichment reduces sediment bacterial and archaeal diversity, composition, and functional profile independent of bioturbator activity.

Eutrophication is a worldwide issue that can disrupt ecosystem processes in sediments. Studies have shown that macrofauna influences sediment processes by engineering environments that constrain microbial communities. Here, we explored the effect of different sizes of the Sydney cockle (Anadara trapezia), on bacterial and archaeal communities in natural and experimentally enriched sediments. A mesocosm experiment was conducted with two enrichment conditions (natural or enriched) and 5 cockle treatments (small, medium, large, mixed sizes and a control). This study was unable to detect A. trapezia effects on microbial communities irrespective of body size. However, a substantial decrease of bacterial richness, diversity, and structural and functional shifts, were seen with organic enrichment of sediments. Archaea were similarly changed although the magnitude of effect was less than for bacteria. Overall, we found evidence to suggest that A. trapezia had limited capacity to affect sediment microbial communities and mitigate the effects of organic enrichment.

The influence of oyster reefs and surrounding sediments on nitrogen removal - An in-situ study along the East coast of Australia.

Oyster reefs play a crucial role in the removal of nitrogen (N) from aquatic systems by facilitating nutrient regeneration and denitrification, both in their tissues and shells and surrounding sediments. However, we still have a limited understanding about the contribution of each component of the reefs (e.g. oysters vs sediments) to N processes, and whether rates are dependent on site-specific characteristics. To address these knowledge gaps, we conducted an experiment across six oyster reefs along 1080 km of the Eastern Australian coast with different sediment characteristics. By using in-situ clear and dark incubation chambers, we assessed how benthic metabolism, nutrient and dinitrogen gas (N2) fluxes varied among the following treatments: 'oysters', 'sediments', and 'sediments + oysters' that were used to represent components of the whole reef habitat (i.e. reef matrix vs surrounding sediments vs the interaction among them, respectively), and sites. We found that during dark conditions and at siltier sites, N2 effluxes from oysters can be up to 23 times higher than sediments, while N2 effluxes from chambers with both sediments and oysters were similar to sediment treatments, and lower than oyster treatments. These results can be explained by sediment processes including nutrient assimilation by benthic microalgae and/or lower nutrient diffusion into interstitial space. Additionally, oyster treatments showed an uptake of nitrate (NO3-) that was likely converted into N2, whereas sediment treatments showed an overall release of NO3-. In dark conditions, ammonium (NH4+) fluxes remained consistent across treatments and sites, indicating that any exports from oyster excretion (in those treatments including oysters) were either counterbalanced by or comparable to exports from sediments. This study provides evidence that the crucial contribution of oyster reefs to N removal is dependent on interactions between reef components and environmental factors.

Response of planktonic microbial assemblages to disturbance in an urban sub-tropical estuary.

Microbes are sensitive indicators of estuarine processes because they respond rapidly to dynamic disturbance events. As most of the world's population lives in urban areas and climate change-related disturbance events are becoming more frequent, estuaries bounded by cities are experiencing increasing stressors, at the same time that their ecosystem services are required more than ever. Here, using a multidisciplinary approach, we determined the response of planktonic microbial assemblages in response to seasonality and a rainfall disturbance in an urban estuary bounded by Australia's largest city, Sydney. We used molecular barcoding (16S, 18S V4 rRNA) and microscopy-based identification to compare microbial assemblages at locations with differing characteristics and urbanisation histories. Across 142 samples, we identified 8,496 unique free-living bacterial zOTUs, 8,175 unique particle associated bacterial zOTUs, and 1,920 unique microbial eukaryotic zOTUs. Using microscopy, we identified only the top <10% abundant, larger eukaryotic taxa (>10 µm), however quantification was possible. The site with the greater history of anthropogenic impact showed a more even community of associated bacteria and eukaryotes, and a significant increase in dissolved inorganic nitrogen following rainfall, when compared to the more buffered site. This coincided with a reduced proportional abundance of Actinomarina and Synechococcus spp., a change in SAR 11 clades, and an increase in the eukaryotic microbial groups Dinophyceae, Mediophyceae and Bathyoccocaceae, including a temporary dominance of the harmful algal bloom dinoflagellate Prorocentrum cordatum (syn. P. minimum). Finally, a validated hydrodynamic model of the estuary supported these results, showing that the more highly urbanised and upstream location consistently experienced a higher magnitude of salinity reduction in response to rainfall events during the study period. The best abiotic variables to explain community dissimilarities between locations were TDP, PN, modelled temperature and salinity (r = 0.73) for the free living bacteria, TP for the associated bacteria (r = 0.43), and modelled temperature (r = 0.28) for the microbial eukaryotic communities. Overall, these results show that a minor disturbance such as a brief rainfall event can significantly shift the microbial assemblage of an anthropogenically impacted area within an urban estuary to a greater degree than a seasonal change, but may result in a lesser response to the same disturbance at a buffered, more oceanic influenced location. Fine scale research into the factors driving the response of microbial communities in urban estuaries to climate related disturbances will be necessary to understand and implement changes to maintain future estuarine ecosystem services.

Exotic asphyxiation: interactions between invasive species and hypoxia.

Non-indigenous species (NIS) and hypoxia (<2 mg O2 l-1 ) can disturb and restructure aquatic communities. Both are heavily influenced by human activities and are intensifying with global change. As these disturbances increase, understanding how they interact to affect native species and systems is essential. To expose patterns, outcomes, and generalizations, we thoroughly reviewed the biological invasion literature and compiled 100 studies that examine the interaction of hypoxia and NIS. We found that 64% of studies showed that NIS are tolerant of hypoxia, and 62% showed that NIS perform better than native species under hypoxia. Only one-quarter of studies examined NIS as creators of hypoxia; thus, NIS are more often considered passengers associated with hypoxia, rather than drivers of it. Paradoxically, the NIS that most commonly create hypoxia are primary producers. Taxa like molluscs are typically more hypoxia tolerant than mobile taxa like fish and crustaceans. Most studies examine individual-level or localized responses to hypoxia; however, the most extensive impacts occur when hypoxia associated with NIS affects communities and ecosystems. We discuss how these influences of hypoxia at higher levels of organization better inform net outcomes of the biological invasion process, i.e. establishment, spread, and impact, and are thus most useful to management. Our review identifies wide variation in the way in which the interaction between hypoxia and NIS is studied in the literature, and suggests ways to address the number of variables that affect their interaction and refine insight gleaned from future studies. We also identify a clear need for resource management to consider the interactive effects of these two global stressors which are almost exclusively managed independently.

Heterogeneity within and among co-occurring foundation species increases biodiversity.

Habitat heterogeneity is considered a primary causal driver underpinning patterns of diversity, yet the universal role of heterogeneity in structuring biodiversity is unclear due to a lack of coordinated experiments testing its effects across geographic scales and habitat types. Furthermore, key species interactions that can enhance heterogeneity, such as facilitation cascades of foundation species, have been largely overlooked in general biodiversity models. Here, we performed 22 geographically distributed experiments in different ecosystems and biogeographical regions to assess the extent to which variation in biodiversity is explained by three axes of habitat heterogeneity: the amount of habitat, its morphological complexity, and capacity to provide ecological resources (e.g. food) within and between co-occurring foundation species. We show that positive and additive effects across the three axes of heterogeneity are common, providing a compelling mechanistic insight into the universal importance of habitat heterogeneity in promoting biodiversity via cascades of facilitative interactions. Because many aspects of habitat heterogeneity can be controlled through restoration and management interventions, our findings are directly relevant to biodiversity conservation.

Legacy Metal Contaminants and Excess Nutrients in Low Flow Estuarine Embayments Alter Composition and Function of Benthic Bacterial Communities.

Coastal systems such as estuaries are threatened by multiple anthropogenic stressors worldwide. However, how these stressors and estuarine hydrology shape benthic bacterial communities and their functions remains poorly known. Here, we surveyed sediment bacterial communities in poorly flushed embayments and well flushed channels in Sydney Harbour, Australia, using 16S rRNA gene sequencing. Sediment samples were collected monthly during the Austral summer-autumn 2014 at increasing distance from a large storm drain in each channel and embayment. Bacterial communities differed significantly between sites that varied in proximity to storm drains, with a gradient of change apparent for sites within embayments. We explored this pattern for embayment sites with analysis of RNA-Seq gene expression patterns and found higher expression of multiple genes involved in bacterial stress response far from storm drains, suggesting that bacterial communities close to storm drains may be more tolerant of localised anthropogenic stressors. Several bacterial groups also differed close to and far from storm drains, suggesting their potential utility as bioindicators to monitor contaminants in estuarine sediments. Overall, our study provides useful insights into changes in the composition and functioning of benthic bacterial communities as a result of multiple anthropogenic stressors in differing hydrological conditions.

Sublethal effects of a rapidly spreading native alga on a key herbivore.

Multiple anthropogenic stressors are causing a global decline in foundation species, including macrophytes, often resulting in the expansion of functionally different, more stressor-tolerant macrophytes. Previously subdominant species may experience further positive demographic feedback if they are exposed to weaker plant-herbivore interactions, possibly via decreased palatability or being structurally different from the species they are replacing. However, the consequences of the spread of opportunistic macrophytes for the local distribution and life history of herbivores are unknown.The green alga, Caulerpa filiformis, previously a subdominant macrophyte on low intertidal-shallow subtidal rock shores, is becoming locally more abundant and has spread into warmer waters across the coast of New South Wales, Australia.In this study, we measured (a) the distribution and abundance of a key consumer, the sea urchin Heliocidaris erythrogramma, across a seascape at sites where C. filiformis has become dominant, (b) performed behavioral field experiments to test the role of habitat selection in determining the local distribution of H. erythrogramma, and (c) consumer experiments to test differential palatability between previously dominant higher quality species like Ecklonia radiata and Sargassum sp. and C. filiformis and the physiological consequences of consuming it.At all sites, urchin densities were positively correlated with distance away from C. filiformis beds, and they actively moved away from beds. Feeding experiments showed that, while urchins consumed C. filiformis, sometimes in equal amounts to higher quality algae, there were strong sublethal consequences associated with C. filiformis consumption, mainly on reproductive potential (gonad size). Specifically, the gonad size of urchins that fed on C. filiformis was equivalent to that in starved urchins. There was also a tendency for urchin mortality to be greater when fed C. filiformis.Overall, strong negative effects on herbivore life-history traits and potentially their survivorship may establish further positive feedback on C. filiformis abundance that contributes to its spread and may mediate shifts from top-down to bottom-up control at locations where C. filiformis has become dominant.

Shifts in biomass and structure of habitat-formers across a latitudinal gradient.

Global patterns of plant biomass drive the distribution of much of the marine and terrestrial life on Earth. This is because their biomass and physical structure have important consequences for the communities they support by providing food and habitat. In terrestrial ecosystems, temperature is one of the major determinants of plant biomass and can influence plant and leaf morphology. In temperate marine systems, macroalgae are major habitat-formers and commonly display highly variable morphology in response to local environmental conditions. Variation in their morphology, and thus habitat structure on temperate reefs, however, is poorly understood across large scales. In this study, we used a trait-based approach to quantify morphological variability in subtidal rocky reefs dominated by the algal genus Sargassum along a latitudinal gradient, in southeastern Australia (~900 km). We tested whether large-scale variation in sea surface temperature (SST), site exposure, and nutrient availability can predict algal biomass and individual morphology. We found Sargassum biomass declined with increasing maximum SST. We also found that individual morphology varied with abiotic ocean variables. Frond size and intraindividual variability in frond size decreased with increasing with distance from the equator, as SST decreased and nitrate concentration increased. The shape of fronds displayed no clear relationship with any of the abiotic variables measured. These results suggest climate change will cause significant changes to the structure of Sargassum habitats along the southeastern coast of Australia, resulting in an overall reduction in biomass and increase in the prevalence of thalli with large, highly variable fronds. Using a space-for-time approach means shifts in morphological trait values can be used as early warning signs of impending species declines and regime shifts. Consequently, by studying traits and how they change across large scales we can potentially predict and anticipate the impacts of environmental change on these communities.

Body size affects lethal and sublethal responses to organic enrichment: Evidence of associational susceptibility for an infaunal bivalve.

Eutrophication is an increasing problem worldwide and can disrupt ecosystem processes in which macrobenthic bioturbators play an essential role. This study explores how intraspecific variation in body size affects the survival, mobility and impact on sediment organic matter breakdown in enriched sediments of an infaunal bivalve. A mesocosm experiment was conducted in which monocultures and all size combinations of three body sizes (small, medium and large) of the Sydney cockle, Anadara trapezia, were exposed to natural or organically enriched sediments. Results demonstrate that larger body sizes have higher tolerance to enriched conditions and can reduce survival of smaller cockles when grown together. Also, large A. trapezia influenced sediment organic matter breakdown although a direct link to bioturbation activity was not clear. Overall, this study found that intraspecific variation in body size influences survival and performance of bioturbators in eutrophic scenarios.

On the diversity and distribution of a data deficient habitat in a poorly mapped region: The case of Sabellaria alveolata L. in Ireland.

Data that can be used to monitor biodiversity through time are essential for conservation and management. The reef-forming worm, Sabellaria alveolata (L. 1767) is currently classed as 'Data Deficient' due to an imbalance in the spread of data on its distribution. Little is known about the distribution of this species around Ireland. Using data archaeology, we collated past and present distribution records and discovered that S. alveolata has a discontinuous distribution with large gaps between populations. Many regions lack data and should be targeted for sampling. Biodiversity surveys revealed that S. alveolata supported diverse epibiotic algal communities. Retrograding (declining) reefs supported greater infaunal diversity than prograding (growing) reefs or sand, suggesting that S. alveolata is a dynamic ecosystem engineer that has a lasting legacy effect. Similar research should be carried out for other Data Deficient species, habitats and regions. Such data are invaluable resources for management and conservation.

Fine-scale responses of mobile invertebrates and mesopredatory fish to habitat configuration.

As habitat-forming species continue to decline globally, it is important to understand how associated communities respond to habitat loss and fragmentation. Changes in the density and spatial configuration of habitat have important consequences for associated communities. However, tests of these factors are often confounded by morphological variation of habitat-formers, which can be resolved by using standardised habitat-mimics. Furthermore, few studies have incorporated the role of predators in mediating the observed effects. To test whether predators mediate the abundance of invertebrates among algal habitats of varying configuration (isolated vs patches, and positions within patches), we placed macroalgal mimics into subtidal estuarine habitats for one month to sample epifaunal communities. At the same time, we conducted underwater video surveys of fish communities to quantify fish communities and their feeding behaviour among the artificial habitats. Isolated habitats did not differ from patch habitats, however, patch edges had the highest epifaunal abundance, where fish were least commonly observed. Observed fish feeding was highest in the middle of patches and increased fish observations and feeding in habitats with reduced epifaunal communities suggest that mesopredatory fish are mediating epifauna in patches, with predation pressure altered by the spatial configuration of the habitat. This contrasts to previous studies that focus on predators that congregate outside patches and suggest that fragmentation leads to reduced invertebrate abundance at habitat edges in contrast to centres. However, this study highlights that in habitat patches housing small mesopredators that also benefit from the increased structure, the centre of the patch experiences higher predation and therefore fewer epifauna in contrast to patch edges and individual algal mimics.

Habitat provided by native species facilitates higher abundances of an invader in its introduced compared to native range.

The impacts invasive species have on biodiversity and ecosystem function globally have been linked to the higher abundances they often obtain in their introduced compared to native ranges. Higher abundances of invaders in the introduced range are often explained by a reduction in negative species interactions in that range, although results are equivocal. The role of positive interactions in explaining differences in  the abundance of invaders between native and invasive ranges has not been tested. Using biogeographic surveys, we showed that the rocky shore porcelain crab, Petrolisthes elongatus, was ~4 times more abundant in its introduced (Tasmania, Australia) compared to its native (New Zealand) range. The habitat of these crabs in the invaded range (underside of intertidal boulders) was extensively covered with the habitat-forming tubeworm Galeolaria caespitosa. We tested whether the habitat provided by the tubeworm facilitates a higher abundance of the invasive crab by creating mimics of boulders with and without the tubeworm physical structure and measured crab colonisation into these habitats at three sites in both Tasmania and New Zealand. Adding the tubeworm structure increased crab abundance by an average of 85% across all sites in both ranges. Our intercontinental biogeographic survey and experiment demonstrate that native species can facilitate invader abundance and that positive interactions can be important drivers of invasion success.

Facilitation of an invader by a native habitat-former increases along interacting gradients of environmental stress.

Native habitat-forming species can facilitate invasion by reducing environmental stress or consumer pressure. However, the intensity of one stressor along a local gradient may differ when expanding the scale of observation to encompass major variations in background environmental conditions. In this study, we determined how facilitation of the invasive porcelain crab, Petrolisthes elongatus, by the native tube-forming serpulid, Galeolaria caespitosa, varied with environmental gradients at local (tidal height) and larger (wave exposure) spatial scales. G. caespitosa constructs a complex calcareous matrix on the underside of intertidal boulders and we predicted that its positive effects on P. elongatus density would increase in intensity with shore height and be stronger at wave-sheltered than wave-exposed locations. To test these predictions, we conducted two experiments. First, we determined the effects of serpulid presence (boulders with live or dead serpulid matrix vs. bare boulders) at six shore heights that covered the intertidal distribution of P. elongatus. Second, we determined the effects of serpulid presence (present vs. absent), shore height (high vs. low) and wave exposure (sheltered vs. exposed) on crabs across six locations within the invaded range in northern Tasmania, Australia. In Experiment 1, the presence of serpulids (either dead or alive) enhanced P. elongatus densities at all shore heights, with facilitation intensity (as determined by a relative interaction index; RII) tending to increase with shore height. In Experiment 2, serpulids facilitated P. elongatus across shore heights and wave exposures, although crab densities were lower at high shore levels of wave-sheltered locations. However, the intensity of crab facilitation by serpulids was greater on wave-sheltered than on wave-exposed shores, but only at the high shore level. This study demonstrates that local effects of native habitat-formers on invasive species are dependent on prevailing environmental conditions at larger spatial scales and that, under more stressful conditions, invaders become increasingly reliant on positive interactions with native habitat-formers. Increased strength of local-scale facilitation by native species, dampening broader scale variations in environmental stressors, could enhance the ability of invasive species to establish self-sustaining populations in the invaded range.

Morphological variation of a rapidly spreading native macroalga across a range of spatial scales and its tolerance to sedimentation.

Understanding how species' traits can shape winners and losers of environmental change can help resolve drivers of current community composition patterns and predict future drivers. Sedimentation is one of the main environmental stressors shaping coastal marine communities and tolerance of high sedimentation rates (e.g. via morphological variation) may allow for competitive dominance. In New South Wales, Australia, the abundance and range of the native green macroalga Caulerpa filiformis have increased over recent decades, apparently associated with sediment disturbance. We used field measurements to test hypotheses about morphological variability in C. filiformis in relation to local- and large-scale environmental variation in water depth, sediment cover and latitude. Using a lab experiment, we tested hypotheses about survival and morphological change under different sedimentation regimes. In the field, C. filiformis fronds were more elongated and less branched when a sediment veneer is present and when water depth increased (i.e. reduced light). At larger spatial scales, frond length and width decreased with increased latitude, but latitude was less important in explaining the variation C. filiformis' length than were depth or sedimentation. Our lab experiment showed a high tolerance to sedimentation, aided by increased investment in vertical growth. This study shows that rapid morphological plasticity is a likely key attribute of the spreading native macroalga C. filiformis. We argue that having a broad environmental tolerance is key to define a species success under environmental change.

The application of bioturbators for aquatic bioremediation: Review and meta-analysis.

Human activities introduce significant contamination into aquatic systems that impact biodiversity and ecosystem function. Many contaminants accumulate, and remediation options are now required worldwide. One method for bioremediation involves the application of macrofauna to stimulate microbial ecosystem processes including contaminant removal. However, if we are to confidently apply such a technique, we need clarity on the effect of bioturbators on different contaminants and how these vary under different environmental scenarios. Here we used a systematic review and meta-analysis to analyse current knowledge on the activities of bioturbating macrofauna in contaminated sediments and quantify how bioturbation-bioremediation changes depend on the taxonomic group, the aquatic ecosystem and important environmental variables. Three common contaminant classes were reviewed and analysed: metals, nutrients (i.e. ammonia and phosphorous) and polycyclic aromatic hydrocarbons (PAH). In addition, meta-regressions were calculated to estimate the effect of environmental and experimental design variables on effect sizes. Meta-analytic results revealed that deeper burrowing and more active sediment surface animals (e.g. polychaetes) increased metal release from sediments, nutrients and oxygen uptake by microbial fractions in comparison to bioturbators that inhabit shallower depths in sediments. In addition, there was a different effect of bioturbators on response variables in different aquatic systems. Finally, bioturbator effects on nutrient and metal release appeared modulated by context-specific variables such as temperature, pH, sediment grain size, animal density and experimental duration. Our findings highlight critical knowledge gaps such as field applications, less studied macrobenthic fauna and the incorporation of molecular approaches. Our results provide the first quantitative synthesis of the effects of bioturbators on contaminant fate and the variables that need to be considered for the optimization of this method as a viable approach for sediment remediation and contaminant management in aquatic systems.

Relationships between the spread of Caulerpa filiformis and fish communities on temperate rocky reefs.

The previously sub-dominant native marine macrophyte Caulerpa filiformis is now dominant on many sub-tidal rocky reefs in New South Wales (NSW), Australia and is expanding its distribution. As C. filiformis is highly chemically defended and structurally different to co-occurring habitat-forming macrophytes, two key attributes that govern fish assemblages, we hypothesized that fish assemblages, particularly herbivorous fishes, would be different at sites where C. filiformis occurred from where it was previously absent and within sites, fish community structure would be correlated to the cover of C. filiformis. We investigated these hypotheses by determining reef-associated fish assemblage attributes (assemblage structure, species richness, total abundance, Shannon-Weiner diversity, abundance of herbivorous species) along transects within sites where C. filiformis was present and absent. Surprisingly, despite large patches and very high densities of C. filiformis on the reefs we sampled, at larger spatial scales (i.e., among sites) no fish assemblage metrics differed between sites with large stands of C. filiformis and sites without the alga. Moreover the abundance of one dominant herbivore, the rock cale Aplodactylus lophodon, was greater at sites within large beds of C. filiformis. At smaller spatial scales, however, i.e. within sites where C. filiformis was present, fish assemblages did vary as a function of C. filiformis cover along transects, although this was not consistent across sampling times. Overall, our results suggest that the potential effects of the spread of this alga on faunal communities warrants further investigation.