RESUMEN
Twelve previously undescribed ophiobolin-type sesterterpenoids, undobolins A-L (1-12), were isolated from Aspergillus undulatus, and their structures were elucidated by spectroscopic analysis, ECD calculations, and single-crystal X-ray diffraction experiments. Compound 1 was the second example of 20-nor-ophiobolin reported, while compounds 2-6 were notable for oxygenation of C-2, and compound 6 showed significant inhibitory activity against ConA-induced T lymphocyte proliferation with an IC50 value of 2.3 µM, which suggests a promising new direction in the quest for immunosuppressive agents.
Asunto(s)
Aspergillus , Sesterterpenos , Sesterterpenos/farmacología , Sesterterpenos/química , Sesterterpenos/aislamiento & purificación , Aspergillus/química , Estructura Molecular , Animales , Linfocitos T/efectos de los fármacos , Cristalografía por Rayos X , Inmunosupresores/farmacología , Inmunosupresores/química , Inmunosupresores/aislamiento & purificación , Ratones , Proliferación Celular/efectos de los fármacosRESUMEN
Germination of soybeans under ultraviolet-B (UV-B) treatment is a simple and effective way to enrich soybean isoflavones, but its mechanism of action is not yet clear. G-Aminobutyric acid (GABA) is a signaling molecule that is involved in the accumulation of secondary metabolites as well as the regulation of plant development and metabolism. In this study, the effects of exogenous GABA and its inhibitors on the physiological and biochemical, antioxidant systems, total flavonoid content, activity and gene expression of isoflavone metabolism related enzyme in germinating soybeans under UV-B treatment were investigated. Compared to UV-B treatment alone, soybean treated with GABA (5 mM) in combination with UV-B significantly increased sprout length, fresh weight, Ca2+ inward flow and peroxidase and catalase activities, and decreased malondialdehyde and H2O2 and O2Ë- fluorescence intensity, while soybean treated with GABA inhibitor showed the opposite trend. Meanwhile, total flavonoid content increased by 11.2% and 6.7%, respectively, in 2- and 4 day-old soybeans under UV-B treatment, compared to UV-B treatment alone. Moreover, the application of GABA under UV treatment significantly increased the activity of phenylalanine ammonia-lyase and cinnamic acid-4-hydroxylase, with values increasing by 43.6% and 18.5%, respectively, in four-day-old soybean compared to UV treatment alone, which also increased the relative expression of key genes involved in isoflavone metabolism. The GABA inhibitor 3-mercaptopropionic acid blocked these occurrences. According to this research, GABA could operate as a signaling molecule to mediate isoflavone accumulation in soybean sprouts under UV radiation and stimulate soybean sprout growth.