RESUMEN
Thymic nurse cells (TNCs) are specialized epithelial cells that reside in the thymic cortex. The initial report of their discovery in 1980 showed TNCs to contain up to 200 thymocytes within specialized vacuoles in their cytoplasm. Much has been reported since that time to determine the function of this heterotypic internalization event that exists between TNCs and developing thymocytes. In this review, we discuss the literature reported that describes the internalization event and the role TNCs play during T cell development in the thymus as well as why these multicellular complexes may be important in inhibiting the development of autoimmune diseases.
Asunto(s)
Formación de la Célula en Célula/fisiología , Células Epiteliales/fisiología , Timocitos/citología , Timocitos/fisiología , Timo/citología , Timo/fisiología , Animales , Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/metabolismo , Autoinmunidad , Comunicación Celular , Diferenciación Celular , Células Epiteliales/ultraestructura , Antígenos de Histocompatibilidad/inmunología , Humanos , Fenotipo , Timocitos/inmunología , Timocitos/ultraestructuraRESUMEN
Both thymic nurse cells (TNCs) and macrophages have been reported to function as antigen-presenting cells during the process of MHC restriction. Negative selection, which results in the apoptosis of potentially autoreactive thymocytes, is believed to be associated with both macrophages and TNCs in the cortex. Both cell types have also been reported to ingest thymocytes undergoing positive and negative selection. However, macrophages ingest apoptotic thymocytes, while TNCs have been shown to internalize viable cells. A subset of the TNC-engulfed population is allowed to mature and is released, while the remaining fraction becomes apoptotic and is absorbed within the TNC cytoplasm through lysosomal activity. A recent report described a subset of rat TNCs that contain macrophages as well as thymocytes within their cytoplasm. We examined freshly isolated TNCs from C57BL/6 mice and found that, of the TNC population recovered, 1.7% contained macrophages within its cytoplasm. There also were macrophages tightly bound but not internalized into the multicellular structure at a rate of 2.9%. The total association of macrophages with TNCs was approximately 4.6%. This unique association of macrophages with TNCs was also observed in vitro when freshly isolated thymocytes (containing macrophages) were added to cultures of cells from the TNC cell line tsTNC-1. The macrophage-TNC interaction was found to be dynamic, with macrophages moving rapidly into and out of TNCs containing cytoplasmic thymocytes. Macrophages within TNCs showed a close association with cytoplasmic thymocytes. We then labeled peritoneal macrophages with CFDA SE, a cell tracking dye, and returned them to the mouse peritoneum. Within 1 h, labeled macrophages were detectable in the thymus. This is the first investigation to show a direct interaction between peripheral macrophages and TNCs. These results suggest that TNCs and macrophages work together as antigen-presenting cells.
Asunto(s)
Presentación de Antígeno , Comunicación Celular/inmunología , Macrófagos Peritoneales/inmunología , Timo/inmunología , Animales , Técnicas de Cocultivo , Ratones , Ratones Endogámicos C57BL , Ratas , Subgrupos de Linfocitos T/inmunología , Timo/citologíaRESUMEN
Since their discovery in 1980, thymic nurse cells (TNCs) have been controversial. Questions pertaining to the existence of the TNC as a "unit" cell with thymocytes completely enclosed within its cytoplasm were the focus of initial debates. Early skeptics proposed the multicellular complex to be an artifact of the procedures used to isolate TNCs from the thymus. Since that time, TNCs have been found in fish, frogs, tadpoles, chickens, sheep, pigs, rats, mice, and humans. Their evolutionary conservation throughout the animal kingdom relieved most speculations about the existence of TNCs and at the same time demonstrated their apparent importance to the thymus and T-cell development. In this review we will discuss and debate reports that describe (i) the organization or structure of TNCs, (ii) the thymocyte subset(s) found within the cytoplasm of TNCs and their uptake and release, and (iii) the function of this fascinating multicellular interaction that occurs during the process of T-cell development. Discussions about the future of the field and experimental approaches that will lead to answers to remaining questions are also presented.
Asunto(s)
Subgrupos de Linfocitos T/inmunología , Timo/inmunología , Animales , Apoptosis , Diferenciación Celular , Citoplasma/inmunología , Citoplasma/ultraestructura , Humanos , Subgrupos de Linfocitos T/citología , Timo/citologíaRESUMEN
Apoptosis of thymocytes associated with thymic nurse cells (TNCs) has been well-documented. TNCs selectively bind and internalize immature alphabeta TCRlo CD4+ CD8+ thymocytes in vitro. A subset of the internalized population matures to the alphabeta TCRhi CD69hi stage of development while the fraction that remains within the cytoplasm dies through the process of apoptosis. Negative selection by thymic cortical epithelial cells has been reported, but little is known about the apoptotic pathway(s) employed to facilitate the death signal. Using the TNC line tsTNC-1 that was reported earlier to maintain the ability to internalize alphabeta TCRlo CD4+ CD8+ cells in vitro, we investigated the role of Fas and TNFalpha in TNC-induced apoptosis. Our initial studies revealed that tsTNC-1 cells express both FasL and TNFalpha apoptosis of triple positive cells was shown to be reduced approximately 50% in co-cultures of tsTNC-1 cells and thymocytes in the presence of either anti-TNFalpha or Fas-Fc. When maximum effective concentrations of both TNFalpha, and Fas-Fc were added to these co-cultures, apoptotic death was further reduced to approximately 68%. These results suggest that both TNFalpha and Fas apoptotic pathways are active during thymocyte selection by TNCs.
Asunto(s)
Apoptosis , Timo/citología , Timo/inmunología , Factor de Necrosis Tumoral alfa/inmunología , Receptor fas/inmunología , Células Epiteliales/inmunología , HumanosRESUMEN
The results of the study presented in this report show that clones of env derived from genetically divergent HIV-1 field isolates fall into two major subsets based on the predicted secondary structure of the V3 region in gp120. One subset exemplified by the clones A-UG06c, B-RT3.12 and C-UG045 is predicted to assume a beta-turn conformation in the V3 loop and comprises the GPGX residues. The other subset exemplified by the clones D-UG23c and D-UG042 (GXGX) are deficient in the expression of the beta-turn in the loop. Since secondary conformations are highly likely to confer antigenic properties in a protein backbone at least for B cells, we have used nucleic acid immunization to test the effect of the beta-turn deficiency on the immunogenic potential of rgp120 encoded in these field isolates. As hypothesized, inoculation of BALB/c mice with the env plasmid encoding the beta-turn expressing rgp120 molecules resulted in the development of a vigorous antibody response to the homologous V3 loop peptides. In contrast, immunization with an rgp120 clone deficient in the beta-turn in the V3 loop showed no evidence of antibody development to the V3 loop. Instead, the latter clones triggered T cell proliferative responses and markedly increased the level of IL-2 and IFN-gamma production by T cells. Significantly, reconstitution of the beta-turn conformation by site-directed mutagenesis of a single V3 loop residue yielded rgp120 molecules which restored antibody production while diminishing the cell-mediated immune (CMI) responses to the V3 residue. These observations demonstrate the marked impact of a single amino acid substitution on the immunogenic properties of V3 region in gp120 encoded by divergent HIV-1 field isolates.
Asunto(s)
Proteína gp120 de Envoltorio del VIH/genética , Proteína gp120 de Envoltorio del VIH/inmunología , VIH-1/genética , Fragmentos de Péptidos/genética , Mutación Puntual , Secuencia de Aminoácidos , Animales , ADN Viral/inmunología , Anticuerpos Anti-VIH/sangre , Proteína gp120 de Envoltorio del VIH/química , VIH-1/química , VIH-1/inmunología , Humanos , Inmunización , Activación de Linfocitos , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Fragmentos de Péptidos/química , Fragmentos de Péptidos/inmunología , Plásmidos/genética , Conformación Proteica , Estructura Secundaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/inmunología , Células TH1/inmunología , Células Th2/inmunologíaRESUMEN
The art of renewal keeps managers and supervisors enthused and excited about what they do. Renewal preserves the sense of purpose that managers and supervisors bring to their jobs and careers. Renewal can be learned and is based on the basic theories of motivation and coping. This article serves as a review for veteran managers and supervisors and as a primer for new managers and supervisors.
Asunto(s)
Agotamiento Profesional/prevención & control , Movilidad Laboral , Personal de Salud/psicología , Adaptación Psicológica , Humanos , MotivaciónRESUMEN
A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alphabetaTCR(lo)CD4(+)CD8(+) thymocytes in vitro was used in long-term coincubation experiments to determine the ultimate fate of thymocytes that remained within intracytoplasmic vacuoles of thymic nurse cells (TNCs). In an earlier report, a subset of the population released from the TNC interaction was shown to mature to the alphabetaTCR(hi)CD69(hi) stage of development, while thymocytes that bided within the TNC cytoplasm died through the process of apoptosis. Here, we show the presence of both apoptotic and nonapoptotic thymocytes within the cytoplasm of freshly isolated TNCs as well as in tsTNC-1 cells in culture. A microscopic analysis revealed total degradation of the cytoplasmic apoptotic thymocyte population that remained in tsTNC-1 cells after an 8- to 10-h incubation period. A quantitative analysis showed an increase of cytoplasmic thymocyte degradation over time to almost 80% after 9 h of incubation. However, in the presence of bafilomycin A1, which is used to inhibit acidification of lysosomal vesicles, degradation of apoptotic thymocytes never reached 10%. These data suggest that lysosomes within TNCs play a role in the degradation of apoptotic thymocytes. We examined tsTNC-1 cells before the addition of thymocytes to cultures and found lysosomes to be clustered around the nucleus in the cytoplasm of TNCs. Shortly after the internalization event, apoptotic thymocytes move to the area of the cytoplasm containing lysosomes. Using the confocal microscope, we obtained evidence that shows the degradation event to be facilitated through the fusion of lysosomes with the specialized vacuoles within TNCs containing apoptotic cells.
Asunto(s)
Apoptosis , Lisosomas/metabolismo , Linfocitos T/citología , Timo/citología , Animales , Degranulación de la Célula , Línea Celular , Células Cultivadas , Técnicas de Cocultivo , Endocitosis , Células Epiteliales/citología , Células Epiteliales/inmunología , Leucocitosis , Lisosomas/inmunología , Ratones , Ratones Endogámicos C57BL , Linfocitos T/inmunología , Timo/inmunologíaRESUMEN
A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ph91 showed specificity to cells of the subcapsular region of the thymic cortex. Upon mechanical dispersion of the thymus in vitro, ph91 recognized cells displaying the multicellular morphology unique to TNCs. Ph91 staining was not detected on fresh thymocytes, stromal cells of the inner thymic cortex, thymic medullary cells, B cells or fibroblasts. Ph91 recognized a 43-kDa protein on the surface of TNCs. Exposure of tsTNC-1 cells to ph91 in tissue culture significantly reduced the percentage of binding of the alpha beta TCRloCD4+CD8+ thymocyte subset previously shown to target TNCs. In organ culture, ph91 reduced the viability of developing thymocytes by 70%. The largest reduction was found in the alpha beta TCR+CD4+CD8+ thymocyte subset. These results represent the first report of a TNC-specific monoclonal antibody. Further, the antigen to which ph91 binds may play a role in the process of thymocyte binding and their subsequent internalization which is unique to TNCs and important to the T cell developmental process.
Asunto(s)
Anticuerpos Monoclonales/química , Especificidad de Anticuerpos , Timo/citología , Timo/inmunología , Animales , Anticuerpos Monoclonales/aislamiento & purificación , Anticuerpos Monoclonales/farmacología , Adhesión Celular/inmunología , Diferenciación Celular/inmunología , Línea Celular Transformada , Células Epiteliales , Epítopos/inmunología , Técnica del Anticuerpo Fluorescente Indirecta , Inhibidores de Crecimiento/farmacología , Ratones , Ratones Endogámicos C57BL , Peso Molecular , Técnicas de Cultivo de Órganos , Especificidad de Órganos/inmunología , Coloración y Etiquetado , Linfocitos T/inmunología , Timo/metabolismoRESUMEN
Burnout is a common phenomenon of today's workplace. Burnout is attributed to stress in the work environment. Today's health care environment with its rapidly changing requirements is an especially obvious example. Prevention of burnout, then, becomes particularly crucial. Certain personality types are resistant to burnout; unfortunately, other personality types are susceptible to burnout. However, managers and supervisors can use knowledge of personality types and their corresponding strengths and vulnerabilities to protect themselves and their subordinates from burnout. There are also general and type-specific coping efforts that people can use.
Asunto(s)
Agotamiento Profesional/prevención & control , Personal de Salud/psicología , Determinación de la Personalidad , Adaptación Psicológica , Femenino , Investigación sobre Servicios de Salud , Humanos , Administración de Personal , Estados Unidos , Lugar de TrabajoRESUMEN
Previous studies have shown that the African strains of HIV-1 mostly cluster with the subtypes A, C or D based on phylogenetic analysis of the ENV nucleotide sequences. In the present investigation we have examined the immunogenic potential of full length gp120 derived from the Ugandan HIV-1 subtype A isolate, AUG06c, using computer-based prediction methods and a plasmid-mediated immunization technique. Computer-assisted analysis of the amino acid residues identified 15 potential B-cell epitopes in gp120 of AUG06c. Despite marked variation in the primary sequences, these epitopes were shown to correspond well to analogous sites in gp120 derived from the subtype B reference clones, MN and IIIBBH10. The relative positions of the epitopes indicated that E9[V3], E14[C3] and E15[V5] correspond to the previously defined principal neutralizing determinant (PND) located in the V3 loop, the CD4 binding site and gp120 "immunodominant" region, respectively. Intramuscular inoculation of BALB/c mice with the ENV clones from AUG06c or from the subtype C clone, CUG045 elicited antibodies which react with the homologous but not with the heterologous PND peptide in ELISA. However, cocktail inoculation with the ENV plasmids from AUG06c and CUG045 elicited antibodies which reacted with both peptides. Antibody response to the other predicted epitopes of AUG06c was not as strong as the response to the PND peptide. The response of the mice to DNA-mediated immunization was further tested in a proliferation assay. Spleen cells derived from the immunized mice exhibited a strong proliferative response to homologous and heterologous PND peptides in [3H]thymidine incorporation assay. DNA-mediated immunization with rgp120 of AUG06c appears to elicit cellular immune response of relatively broad specificity.
Asunto(s)
Proteína gp120 de Envoltorio del VIH/inmunología , VIH-1/inmunología , Secuencia de Aminoácidos , Animales , Clonación Molecular , Proteína gp120 de Envoltorio del VIH/genética , VIH-1/genética , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Plásmidos , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Vacunas ViralesRESUMEN
A temperature-sensitive line of thymic nurse cells (tsTNC-1) that maintains the ability to selectively internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in long-term coincubation experiments to determine nurse cell function during the process of MHC restriction. The thymocyte subset released from its association with TNCs contained both viable and apoptotic cells. The cells that remained within intracytoplasmic vacuoles died through the process of programmed cell death. Surviving or rescued thymocytes in the released population displayed an increase in Bcl-2 protein expression. The rescue activity of TNCs was drastically reduced with the addition of antibodies against either class I or class II MHC antigens to cocultures. A subset of the TNC-rescued population matured from the alpha beta TCRloCD69- phenotype to alpha beta TCRhiCD(69+)-expressing cells only when IL-1 beta was added to cocultures. These results suggest that TNC rescue of early double-positive thymocytes from apoptosis is associated with an interaction between the TCR and the MHC and the onset of Bcl-2 expression. Maturation of thymocytes within the TNC-rescued population requires the costimulatory effects of IL-1 beta.
Asunto(s)
Interleucina-1/farmacología , Activación de Linfocitos/efectos de los fármacos , Proteínas Proto-Oncogénicas/biosíntesis , Timo/citología , Timo/inmunología , Animales , Antígenos de Diferenciación de Linfocitos T/análisis , Apoptosis/inmunología , Inmunofenotipificación , Activación de Linfocitos/inmunología , Ratones , Ratones Endogámicos C57BL , Proteínas Proto-Oncogénicas c-bcl-2 , Linfocitos T/clasificación , Linfocitos T/metabolismo , Timo/metabolismoRESUMEN
We have now developed temperature sensitive lines of thymic nurse cells (TNCs), using the SV40 viral mutant tsA58, that maintain the ability to selectively internalize a subpopulation of alpha beta TCR+CD4+CD8+ thymocytes in vitro. One line, tsTNC-1, was shown to be able to rescue a subset of CD4+CD8+ thymocytes from programmed cell death at 32 degrees C, the temperature at which binding and internalization were detected. Rescue was significantly diminished at 38 degrees C, the temperature at which thymocyte binding was not observed. The rescued population of thymocytes showed a reduced level of apoptosis as measured by the DNA fragmentation assay. TNC rescue resulted in a shift of CD4+CD8+ thymocytes from immature TCRlow PNArhigh cells to the more mature TCRint PNArlow phenotype but no changes in cell surface levels of HSA nor CD69 were detected. The rescue activity of tsTNC-1 cells at 32 degrees C was significantly reduced with the addition of antibodies to either class I or class II MHC antigens. These results suggest that we have established TNC lines, using the SV40 viral mutant tsA58, that have the ability to rescue a subset of the TNC interactive thymocyte population from programmed cell death. The thymocyte population rescued by TNCs matures to a phenotype within the double positive stage of development that is indicative of positive selection.
Asunto(s)
Apoptosis/inmunología , Subgrupos de Linfocitos T/citología , Subgrupos de Linfocitos T/inmunología , Timo/citología , Timo/inmunología , Animales , Antígenos CD4/metabolismo , Antígenos CD8/metabolismo , Diferenciación Celular/inmunología , Línea Celular , Supervivencia Celular , ADN/metabolismo , Ratones , Microscopía Electrónica , Microscopía Electrónica de Rastreo , Mutación , Fenotipo , Receptores de Antígenos de Linfocitos T alfa-beta/metabolismo , Virus 40 de los Simios/genética , Subgrupos de Linfocitos T/metabolismo , Temperatura , Timo/metabolismo , Vacuolas/ultraestructuraRESUMEN
Synthetic peptides comprising the predicted principal neutralizing determinant (PND) in new African and North American HIV-1 clones were tested in ELISA for reactivity with ninety six serum samples from asymptomatic donors in six selected localities in Uganda. Irrespective of the geographical origin of the samples, the majority of the test sera cross-reacted at high intensities with the peptides derived from the North American clone, BRT3.6 (Group B), the Ugandan clone, CUG045, (Group C), and the Romanian clone, FRMA (Group F). The frequency of reactivity of the peptides from BRT3.6, CUG045, and FRMA were within the ranges of 57-100%, 50-100%, and 57-100%, respectively, for the sera collected from these disparate localities. In contrast to these findings, the V3 peptides derived from the other Ugandan isolates showed a more restricted pattern of reactivity with the same serum samples: AUG06c (1-63%), DUG23c (2%), and DUG044 (38-87%). The results from ELISA inhibition assay indicated that the V3 peptide from BRT 3.6, CUG045, and FRMA express closely related antigenic specificities quite distinct from those in AUG06c and DUG044. The residues comprising the PND in BRT 3.6, CUG045, and FRMA appear to be well conserved in the HIV-1 subtypes prevalent in the selected Ugandan locales.
PIP: In Uganda, health workers collected serum samples from 96 asymptomatic HIV-1 infected blood donors in Ishaka and Mbarara (southwest), Kisenyi and Kampala (central), and Lugazi and Jinja (east) so researchers working in a biochemistry laboratory at The City University of New York could describe the relative reactivity of the V3 loop from HIV-1 subtypes A, B, C, D, and F, as well as study the antigenic relationships within the PND encoded in the divergent HIV-1 subtypes. Regardless of geographic origin, the V3 peptides from most of the sera (at least 50%) collected in Uganda cross-reacted at high frequencies with the peptides derived from the novel North American clone (BRT3.6), the Ugandan clone (CUG045), and the Romanian clone (FRMA). The frequency of reactivity of these peptides with the test sera ranged from 57% to 100% for BRT3.6, from 50% to 100% for CUG045, and from 57% to 100% for FRMA. The V3 peptides from other Ugandan isolates (AUG06c, DUG044, and DUG23c) were less reactive with the same serum samples than BRT3.6, CUG045, and FRMA: 1-63%, 38-87%, and 2%, respectively. This finding suggests that the antigenic determinants expressed in AUG06c, DUG044, and DUG23c may not represent the PND encoded in most HIV-1 strains afflicting the Ugandan communities. The V3 peptides from BRT3.6, CUG045, and FRMA express closely related antigenic specificities altogether different from those in AUG06c and DUG044. The HIV-1 subtypes present in the selected Ugandan sites appear to effectively conserve the residues making up the PND in BRT3.6, CUG045, and FRMA.
Asunto(s)
Anticuerpos Anti-VIH/inmunología , Proteína gp120 de Envoltorio del VIH/inmunología , Infecciones por VIH/virología , VIH-1/inmunología , Fragmentos de Péptidos/inmunología , Secuencia de Aminoácidos , Reacciones Cruzadas , Ensayo de Inmunoadsorción Enzimática , Epítopos , Infecciones por VIH/inmunología , VIH-1/clasificación , Humanos , Datos de Secuencia Molecular , New York , Filogenia , Rumanía , UgandaRESUMEN
We have initiated studies to characterize the predominant subtypes of HIV-1 which account for infections in a defined cohort of intravenous (IV) drug addicts. A region of ENV encoding the C2 to the V5 regions was amplified from the leukocytes of two subjects currently enrolled in a methadone maintenance program at the Addiction Research and Treatment Corporation (ARTC), in Brooklyn, New York. This region of the viral genome encodes the principal neutralizing determinant (PND) located in the V3 loop, the immunogenic CD4-binding site, and six other linear antigenic epitopes in the envelope glycoprotein, gp120. Phylogenetic tree analysis of the nucleotide sequences showed that the sibling clones RT1.4, RT1.15, RT1.17, RT1.21 and RT3.6, RT3.10, RT3.11, RT3.12 and RT3.15 derived from the isolates, RT1 and RT3, respectively, cluster with "group B" viruses at 99% confidence level. Marked intra-patient and inter-patient sequence variation was apparent in the V3 loop. The divergence included the presence of a previously unreported hexapeptide GPWGTF at the cap of the loop in the clones from RT1. The North American consensus hexapeptide, GPGRAF, was identified in the cap of the loop from the clones of RT3. Four of the five sibling clones from RT3 were closely related whereas the other clone, RT3.15, displayed five amino acid mutations downstream of the V3 cap. To assess the effect of sequence variation on the distribution of linear antigenic epitopes, complementary computer software programs, were used to analyze the gp120 residues. Eight analogous antigenic epitopes were identified in the clones from both isolates despite the marked divergence in the primary sequences.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Variación Antigénica/genética , Epítopos/inmunología , Proteína gp120 de Envoltorio del VIH/inmunología , Infecciones por VIH/virología , VIH-1/inmunología , Secuencia de Aminoácidos , Estudios de Cohortes , Comorbilidad , Ensayo de Inmunoadsorción Enzimática , Anticuerpos Anti-VIH/sangre , Anticuerpos Anti-VIH/inmunología , Proteína gp120 de Envoltorio del VIH/química , Proteína gp120 de Envoltorio del VIH/genética , Infecciones por VIH/epidemiología , Infecciones por VIH/transmisión , VIH-1/clasificación , VIH-1/genética , VIH-1/aislamiento & purificación , Humanos , Datos de Secuencia Molecular , Ciudad de Nueva York/epidemiología , Fragmentos de Péptidos/genética , Fragmentos de Péptidos/inmunología , Filogenia , Reacción en Cadena de la Polimerasa , Estructura Terciaria de Proteína , Factores de Riesgo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Centros de Tratamiento de Abuso de Sustancias/estadística & datos numéricos , Abuso de Sustancias por Vía Intravenosa/epidemiologíaRESUMEN
Two major epitopes expressed in HIV-1 have been recently shown to play a central role in virus neutralization. One of these important specificities is a type-specific or group-specific, principal neutralizing determinant (PND) located in the V3 loop of gp120. The other is a more broadly neutralizing determinant associated with the CD4 binding site. Structural and serological studies of the variation in these epitopes have become important in vaccine research. This report describes the analysis of the DNA clones encoding a region of gp120 that overlaps the V3 loop and the putative CD4 recognition site in two new African isolates, UG06c and UG23c. Phylogenetic analyses of the DNA sequences showed that the new African isolates clustered with two very distinct subtypes of HIV-1. UG06c was grouped with U455, D687, and Z321, previously classified as "HIV-1 subtype A" in the AIDS and human retroviruses database; and UG23c was grouped with MAL, JY1, NDK, ELI, and Z2Z6 classified as "HIV-1 subtype D." Considerable variation was apparent in the V3 loop. The divergence included the presence of the hexapeptides GP-GRSF and GLGQAL at the cap of the loop in UG06c and UG23c, respectively. The GPGR tetrapeptide in UG06c formed a beta-turn configuration similar to that of MN or IIIB. The beta-turn was not found to be a likely conformation for GLGQ. The amino acids previously implicated in CD4 binding and the associated neutralizing activity were relatively conserved. To assess a possible impact of the sequence and conformational variations on serological reactivity, UG06c and UG23c were subjected to neutralization assay.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Antígenos CD4/química , Proteína gp120 de Envoltorio del VIH/química , Infecciones por VIH/microbiología , VIH-1/genética , Fragmentos de Péptidos/química , Estructura Secundaria de Proteína , Secuencia de Aminoácidos , Secuencia de Bases , Sitios de Unión , Antígenos CD4/genética , Antígenos CD4/inmunología , Clonación Molecular , Estudios de Cohortes , Reacciones Cruzadas , ADN Viral/química , Epítopos/química , Epítopos/genética , Epítopos/inmunología , Variación Genética , Proteína gp120 de Envoltorio del VIH/genética , Proteína gp120 de Envoltorio del VIH/inmunología , VIH-1/clasificación , VIH-1/inmunología , Humanos , Sueros Inmunes/inmunología , Masculino , Datos de Secuencia Molecular , Pruebas de Neutralización , Ciudad de Nueva York , Fragmentos de Péptidos/genética , Fragmentos de Péptidos/inmunología , Filogenia , Programas Informáticos , UgandaRESUMEN
Recent studies in our laboratory have described the development of the SV40-transformed thymic nurse cell (TNC) line SVT-II2, that maintains the ability to internalize thymocytes in vitro. SVT-II2 cells were shown to bind and internalize a subset of the alpha beta TCR+, CD4+CD8+ thymocyte population exclusively. Also, SVT-II2 cells express cell surface class I and class II MHC antigens. These data are consistent with reports that suggest that TNCs may have a role in thymic education. In this report, we used scanning electron microscopy, transmission electron microscopy, and long-term video microscopy to study binding, internalization, and release of thymocytes by TNCs. The results of these experiments showed the internalization event to be selective and dynamic. The process appears to involve programmed cooperation between the two cell types that terminates with the release of selected thymocytes. Although no changes in thymocyte cell surface phenotype were detected as a result of their interaction with TNCs in vitro, over 90% remained viable after a 48-hr incubation period.
Asunto(s)
Subgrupos de Linfocitos T/citología , Timo/citología , Animales , Línea Celular , Citoplasma/ultraestructura , Endocitosis , Técnicas In Vitro , Ratones , Ratones Endogámicos C57BL , Microscopía Electrónica , Microscopía Electrónica de Rastreo , Grabación en VideoRESUMEN
Thymic nurse cells (TNC) contain 20-200 thymocytes within specialized vacuoles in their cytoplasm. The purpose of the uptake of thymocytes by TNCs is unknown. TNCs also have the capacity to present self-antigens, which implies that they may serve a function in the process of thymic education. We have recently reported the development of thymic nurse cell lines that have the ability to bind and internalize T cells. Here, we use one of these TNC lines to identify the thymocyte subpopulation(s) involved in this internalization process. TNCs exposed to freshly isolated thymocytes bind and internalize CD4 and CD8 expressing thymocytes (CD4+CD8+ or double positives) exclusively. More specifically, a subset of the double-positive thymocyte population displayed binding capacity. These double-positive cells express cell surface alpha beta type T cell antigen receptor (TCR), as well as CD3 epsilon. Binding was not inhibited in the presence of antibodies against CD3, CD4, CD8, Class I antigens, or Class II antigens. These results describe two significant events in T cell development. First, TNCs exclusively bind and internalize a subset of alpha beta TCR expressing double-positive T cells. Also, binding is facilitated through a mechanism other than TCR recognition of major histocompatibility complex antigens. This suggests that thymocyte internalization may be independent of the process used by TNCs to present self-antigen.
Asunto(s)
Antígenos CD/inmunología , Antígenos de Histocompatibilidad/inmunología , Receptores de Antígenos de Linfocitos T/inmunología , Linfocitos T/inmunología , Timo/inmunología , Animales , Antígenos CD4/inmunología , Antígenos CD8/inmunología , Línea Celular Transformada , Femenino , Técnica del Anticuerpo Fluorescente , Masculino , Ratones , Ratones Endogámicos C57BLRESUMEN
Thymic nurse cells (TNCs) are stromal elements that contain between 20 and 200 T cells within their cytoplasm. Because of this unique feature they are believed to play a role in thymocyte development. Unfortunately, it has been difficult to obtain pure TNCs in quantities sufficient for extensive evaluation of their thymic function. As a result, only a limited amount of information is available that characterizes TNCs or the T cell population(s) found within their cytoplasm. We have now used SV40 to infect and immortalize TNCs from C57BL/6 mice. SV40-transformed TNCs were found to specifically bind and internalize cells from an immature thymocyte line isolated in our laboratory. These results describe a method of obtaining pure populations of TNCs for future studies of their thymic function, and suggest that binding to specific subpopulations of lymphoblasts may be necessary for internalization.
Asunto(s)
Transformación Celular Viral , Virus 40 de los Simios/genética , Linfocitos T/fisiología , Timo/citología , Animales , Separación Celular/métodos , Ratones , Ratones Endogámicos C57BL , Fenotipo , Linfocitos T/inmunologíaRESUMEN
The gene that produces the proteolytic enzyme, tissue plasminogen activator (tPA), was used as a selectable marker for transfection. Cells that were successfully transfected with plasmids containing a gene for tPA (tpa) produce plaques in the caseinolysis assay. Two tpa-containing plasmids were constructed and used to transfect a variety of cell types. One plasmid contained the promoter region of simian virus 40 and the other contained the Moloney murine leukemia virus promoter. No significant difference in transfection frequencies was found for the two plasmids. However, 80% of the cell types tested produced plaques. This system allows for the identification and isolation of transfected cells under conditions that are not lethal to nontransfected cells.
Asunto(s)
Activador de Tejido Plasminógeno/genética , Transfección , Animales , Caseínas/metabolismo , Línea Celular , Expresión Génica , Humanos , Virus de la Leucemia Murina de Moloney/genética , Plásmidos , Regiones Promotoras Genéticas , Protoplastos/análisis , Ratas , Virus 40 de los Simios/genética , Activador de Tejido Plasminógeno/biosíntesisRESUMEN
Antibody-directed targeting of vesicles to cells dramatically enhances polyethylene glycol-mediated fusion and microinjection. Sealed erythrocyte ghosts or liposomes, containing fluorescent bovine serum albumin, were targeted to murine spleen and thymus cells, and to lymphocyte and monocyte cell lines. In all cases, targeted cell populations showed substantial levels of microinjection, whereas populations treated with the fusogen in the absence of targeting were not significantly microinjected. Attachment of vesicles to selected cells was achieved by first labelling the cells with biotin-modified antibody and then treating them with avidin-coupled sealed ghosts or liposomes. Another approach to the promotion of selective fusion aims to alter the cell recognition properties of Sendai virus so that its fusogenic activity may be redirected to specific cellular targets. The agglutination and fusion of red cells by UV-inactivated Sendai virus were completely blocked by low concentrations of a Fab preparation of a monoclonal antibody against the viral haemagglutinin (HN) sites. Agglutination and fusion activity were restored in the presence of Fab-anti-HN by providing an alternative recognition system, namely, when the virus had been coupled with biotin and the red cells with avidin. Methods for facilitating microinjection by specifically directing vesicles to target cells may be particularly useful in overcoming barriers to the transfer of genes into lymphocytes by standard transfection techniques.