Effect of thermocycling on surface topography and fracture toughness of milled and additively manufactured denture base materials: an in-vitro study.

BACKGROUND: Studies investigating thermocycling effect on surface topography and fracture toughness of resins used in digitally manufactured denture bases are few. The study aimed to assess the impact of thermocycling on surface topography and fracture toughness of materials used for digitally manufactured denture bases. METHODS: Water sorption, solubility, hardness, surface roughness, and fracture toughness of both three-dimensional (3D)-printed and computer-aided design, computer-aided manufacturing (CAD-CAM) milled specimens (n = 50) were assessed both prior to and following 2000 thermocycles, simulating 2 years of clinical aging. Surface hardness (n = 10) was measured using a Vickers hardness testing machine, surface roughness (n = 10) was determined by a contact profilometer, and fracture toughness (n = 20) was measured using the 3-point bend test, then studying the fractured surfaces was done via a scanning electron microscope (SEM). Prior to and following thermocycling, water sorption and solubility (n = 10) were assessed. Normally distributed data was tested using two-way repeated ANOVA and two-way ANOVA, while Mann Whitney U test and the Wilcoxon signed ranks test were used to analyze data that was not normally distributed (α < 0.05). RESULTS: Following thermocycling, Vickers hardness and fracture toughness of both groups declined, with a significant reduction in values of the 3D-printed resin (P < .001). The 3D-printed denture base resins had a rougher surface following thermocycling with a significant difference (P < .001). The sorption and solubility of water of both materials were not affected by thermocycling. CONCLUSIONS: Before and after thermocycling, milled specimens had lower surface roughness and a greater degree of hardness and fracture toughness than 3D-printed specimens. Thermocycling lowered hardness and fracture toughness, and increased surface roughness in both groups, but had no effect on water sorption and solubility.

Different wavelengths of laser: are they significant for treatment of denture stomatitis?: an in-vitro study.

BACKGROUND: Denture stomatitis (DS) is an inflammatory disorder that affects the mucosal surface underneath the dentures and frequently causes oral mucosal irritation, discomfort, and altered taste perception, which prevents people from consuming enough nutrients. One of the main causes of DS is an overgrowth of the fungus Candida albicans (C. albicans). A possible alternative treatment for Candida infections is thought to be laser therapy. The aim of this study was to evaluate how different wavelengths of laser would affect growth and pathogenic properties of Candida albicans. METHODS: A concentration of 106 viable cells/ml of Candida albicans were used in the preparation process. Four groups were created from the specimens. Culturing of the control group was completed with no intervention. The other 3 groups received laser radiation for 60 seconds at a power of 1W. The 2nd and 3rd groups were irradiated with diode laser at a wavelength of 940 nm and 980 nm respectively. The 4th group was irradiated with Nd-YAG laser at a wavelength of 1064 nm. Turbidimetric growth was defined as variations in the optical density of fungal growth. These measures were made at three different times: baseline, 48 hours, and 72 hours. RESULTS: In both groups of diode laser, the growth of Candida albicans showed no remarkable differences at baseline, after 48 and 72 hours using a power of 1 W and duration of 60 seconds. The Nd-YAG group showed significant increase in optical density after 48 hrs then significant decrease after 72 hrs. The optical density values in the control group showed no notable difference between the control and diode study groups at different time periods. However, the Nd:YAG group showed a statistically significant difference compared to the control and the 2 diode laser groups. CONCLUSIONS: Different laser parameters have a different effect on growth and pathogenic properties of Candida albicans. Diode laser therapy with wavelengths 940 and 980 nm used in continuous mode with power of 1 W for duration of 60 seconds can result in proliferation of Candida albicans instead of destroying them. Nd:YAG laser, used in pulsed mode, with power of 1 W for a duration of 60 seconds can be used to destroy Candida albicans and therefore, can be used as an effective treatment for denture stomatitis.

Effect of denture cleansers on the physical and mechanical properties of CAD-CAM milled and 3D printed denture base materials: An in vitro study.

STATEMENT OF PROBLEM: Studies on the impact of denture cleansers on the physical and mechanical properties of denture bases designed and constructed by using computer software programs are lacking. PURPOSE: The purpose of this in vitro study was to test the effect of a peroxide denture cleanser on the hardness, fracture toughness, water sorption, and solubility of denture base materials manufactured by 3D printing and computer-aided design and computer-aided manufacturing (CAD-CAM) milling. MATERIAL AND METHODS: The hardness, fracture toughness, water solubility, and sorption of CAD-CAM milled and 3D printed groups (n=40) were evaluated before and after exposure to a denture cleanser. Hardness (n=10) was analyzed with a Vickers hardness testing machine, and fracture toughness (n=20) with the 3-point bend test. After the fracture of specimens, a scanning electron microscope at ×300 was used for fractographic analysis. Water sorption and solubility (n=10) were evaluated before and after immersion in denture cleanser for 6 days to simulate 180 days of immersion. Two-way repeated ANOVA and 2-way ANOVA were used to test normally distributed data, whereas the Mann Whitney U test and Wilcoxon signed ranks test were used for data that were not normally distributed (α<.05). RESULTS: The Vickers hardness and fracture toughness of both materials decreased after immersion in denture cleansers, with a higher decrease in values for the 3D printed group (P<.001). The denture cleanser had no effect on the water sorption and solubility of either group. CONCLUSIONS: Milled specimens had higher hardness values and fracture toughness before and after immersion in the denture cleanser. Denture cleansers resulted in the reduced hardness and fracture toughness of both groups, but the percentage change in the milled group was lower. Denture cleansers had no effect on water sorption or solubility.

Evaluation of physicomechanical properties of milled versus 3D-printed denture base resins: A comparative in vitro study.

STATEMENT OF PROBLEM: Studies comparing the physicomechanical characteristics of denture base resins manufactured by computer-aided design and computer-aided manufacturing (CAD-CAM) milling and 3-dimensional (3D) printing are sparse, resulting in challenges when choosing a fabrication method for complete dentures. PURPOSE: The purpose of this in vitro study was to evaluate and compare the impact strength, flexural strength, and the surface roughness of denture base resins manufactured by CAD-CAM milling and 3D printing before and after thermocycling and polishing. MATERIAL AND METHODS: Evaluation of the physicomechanical properties (n=35) was completed before and after 500 thermocycles. Impact strength (n=14) was measured with a Charpy impact tester and flexural strength (n=14) with the 3-point bend test. Surface roughness (Ra) was evaluated (n=7) with a profilometer before and after thermocycling and polishing and by viewing the surface topography before and after polishing using a scanning electron microscope at ×2000. The Mann-Whitney U test and Wilcoxon sign rank test were used for statistical analysis (α=.05). RESULTS: Milled specimens showed statistically significantly higher impact strength before thermocycling and statistically significantly higher flexural strength before and after thermocycling (P=.004) compared with 3D-printed specimens. The Ra values for the milled group were significantly lower than for the 3D-printed group both before and after thermocycling (P=.006) and after polishing (P=.027). Thermocycling resulted in a statistically significant difference in flexural strength (P=.018) in both groups and in surface roughness in the milled group (P=.048); but no significant effect was found on impact strength (P>.05). Ra values for the 3D-printed group decreased after polishing (P=.048). CONCLUSIONS: Milled specimens had higher flexural and impact strength and lower surface roughness values than 3D-printed specimens. Polishing significantly reduced the surface roughness in 3D-printed specimens but had no significant effect on milled specimens.

A prospective cross-over study to evaluate the effect of two different occlusal concepts on the masseter muscle activity in implant-retained mandibular overdentures.

BACKGROUND: The purpose of this prospective cross-over study is to evaluate the effect of bilateral balanced occlusion and canine guidance occlusion on the masseter muscle activity using implant-retained mandibular overdentures. METHODS: After evaluation of 12 completely edentulous patients using cone beam computed tomography (CBCT), mucoperiosteal flaps were reflected exposing the mandibular interforaminal region. Two implants were placed in the interforaminal region for each of the 12 patients. After a healing period of 3 months, acrylic maxillary complete dentures and mandibular overdentures were fabricated with bilateral balanced occlusion for 6 patients and canine guidance occlusion for the other 6 patients. Electromyographic evaluation of the masseter muscles, during clenching on a silicon index and chewing peanuts and cake, was conducted on the patients after using their dentures for 4 weeks. Each occlusion concept was then converted into the other concept using the same dentures, and the procedure of evaluation was repeated after 4 weeks. The recordings were analyzed statistically using Wilcoxon signed ranks test. p < 0.05 was considered statistically significant. RESULTS: The highest electromyographic activity of the masseter muscles was recorded during clenching on a preformed silicon index followed by chewing peanut then cake for both occlusal concepts. The recordings of the masseter muscle associated with canine guidance occlusion were higher than bilateral balanced occlusion but with no statistically significant difference except between the right masseter muscles during clenching (p = 0.042*). CONCLUSIONS: Both bilateral balanced occlusion and canine guidance occlusion can be used successfully in implant-retained mandibular overdentures without affecting masseter muscle activity.