The impacts of individual and combined exposure to cadmium and lead on intraocular pressure, electroretinography, and residual changes in the rabbit eyes.

The human eye is very vulnerable to various environmental pollutants. Cadmium (Cd) and lead (Pb) are widely spread heavy metals. The goal of the existing study is to explore the impact of single or joint exposure to Cd and Pb on the eye indicators. In this study, male New Zealand white rabbits were treated orally for 30 days with Cd (5 mg Cd Cl2/kg bw) associated or not with Pb (12.5 mg lead acetate/kg bw). Fundus and slit lamp examinations, electroretinography (ERG), intraocular pressure (IOP), Cd and Pb residues, and the histopathological picture of the eye were studied. The results revealed that the oral dosing of Cd or Pb evoked a significant (p < 0.05) decline in a- and b-wave amplitudes, under scotopic conditions, and IOP values. Single Pb or Cd treatment showed a significant (p < 0.001) increase in their residues in the whole eye tissue of the Pb- or Cd-treated group. Eye structures of Cd- or Pb-intoxicated rabbit showed mild degenerated changes of cornea and sclera tissues with the presence of irregular variably sized eosinophilic droplets in the lens. Notably, the simultaneous exposure to Cd and Pb leads to an antagonistic outcome in all of the estimated parameters. These findings concluded that oral exposure to Cd or Pb could significantly disturb the vision but their joint exposure caused an opposing effect on nearly all of these disturbances.

Morphological, biochemical, and histopathological postmortem ocular indices following subchronic exposure to cadmium and/or lead in a rabbit model.

Cadmium (Cd) and lead (Pb) are ubiquitous environmental pollutants. There is a dearth of information on the mutual interaction between the antemortem metal intoxication and the postmortem changes of the eye. Thus, this study aimed to follow the morphological, biochemical, histopathological ocular perturbations and the retinal DNA damage up to 8 h postmortem (PM) in Cd and/or Pb intoxicated rabbits. The animals orally received 5 mg Cd Cl2/kg bw and/or 12.5 mg lead acetate/kg bw for 30 consecutive days. At time of death, eye pupil of different groups had a normal diameter except Pb-intoxicated group had marked myosis. After 8 h of death, different rabbit's eye corneas appeared wrinkled and covered with thin white cloud while the pupils were in the mydriatic stage. Up to 8 h PM, the individual exposure to Cd or Pb resulted in a significant elevation in GGT, urea, K, DNA damage and obvious retinal lesions. However, their co-exposure evoked an antagonistic outcome. The eye of Cd and/or Pb intoxicated rabbit showed mildly degenerated tissue of cornea and sclera and the presence of irregular eosinophilic droplets of variably size in the lens with a gradual degeneration and vacuolization in the different cell layers of retina especially ganglion up to 8 h PM. Also, by increasing post mortem interval (PMI), retinal DNA damage in Cd and/or Pb intoxicated group significantly decreased. It is concluded that Cd and/or Pb intoxication induced ocular alterations which retain the same trend in correlation with PMI as natural deaths except for the retinal DNA damage. Also, the simultaneous exposure to Cd and Pb evoked an antagonistic outcome in the eye. The findings of the current study should be taken into consideration when estimating PMI in areas with high Cd and/or Pb contamination.