Modified EASIX scores predict severe CRS/ICANS in patients with acute myeloid leukemia following CLL1 CAR-T cell therapy.

Chimeric antigen receptor T (CAR-T) cell therapy targeting CLL1 has been considered a potent weapon for patients with acute myeloid leukemia (AML). This study aims to evaluate the efficacy and toxicity of CLL1 CAR-T cell therapy in a larger cohort, with particular attention to cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). Among the 32 patients assessed for efficacy, complete remission occurred in 71.88% (23/32) of cases and undetectable minimal residual disease in 14 patients. The CRS developed in all patients, with 8 individuals experiencing ICANS. Severe CRS and ICANS were observed in 11 and 2 patients, respectively. Furthermore, the Endothelial Activation and Stress Index (EASIX) and its derivatives measured before and after CLL1 CAR-T cell infusion were employed for predicting the severe complications. Significant differences were observed in EASIX scores on the day before lymphodepletion (Day BL, P = 0.023), -1 (P < 0.001), +1 (P < 0.001), and +3(P = 0.014); sEASIX scores on Day BL (P = 0.007), -1 (P < 0.001), +1 (P < 0.001), and +3 (P < 0.001); and mEASIX score on Day -1 (P = 0.004) between patients with mild and severe CRS/ICANS. Additionally, there was a significant difference in mEASIX scores between responders and non-responders on Day BL (P = 0.004) and Day -1 (P = 0.044). Our findings indicate that pre- and post-infusion assessments of EASIX/mEASIX/sEASIX scores serve as reliable prognostic indicators for severe CRS/ICANS and treatment response following CLL1 CAR-T cell therapy, which can assist physicians in implementing preemptive treatment strategies for potential severe complications and screening patients who are suitable candidates for CLL1 CAR-T cell therapy. EASIX/mEASIX/sEASIX scores serve as reliable prognostic indicators for severe CRS/ICANS following CLL1 CAR-T cell therapy. The preinfusion mEASIX scores of CLL1 CAR-T cells can effectively predict treatment response.

Functional, transcriptomic, and lipidomic studies of the choC gene encoding a phospholipid methyltransferase in Aspergillus fumigatus.

IMPORTANCE: This study explored the phospholipid metabolic pathway in A. fumigatus and its relationship with fungal growth, metabolism, and pathogenicity. ChoC, based on its critical roles in many aspects of the fungus and relatively conserved characteristics in filamentous fungi with low similarity with mammalian ones, can be a novel target of new antifungal drugs.

In-plane ESPI with unlimited angle of view for multi-object rotation angle determination.

The in-plane electronic speckle pattern interferometry (ESPI), implemented in a Michelson stellar interferometer-like configuration, offers high sensitivity and dynamic measurement. However, its limited angle of view (AOV) remains a major challenge for the rotation angle determination of multiple objects. In this Letter, we analyze the main factors that influence the AOV of the in-plane ESPI and propose an "image transmitting" approach to enlarge the AOV. With the aid of a folded dual-telescope imaging system, we develop an AOV-unlimited interferometer that can determine multi-object rotation angles in real time. The practicability of the interferometer is demonstrated by the application in real-time measuring of the rotation angles of the disks within a 2D granular system.

Efficacy and safety of different anti-osteoporotic drugs for the spinal fusion surgery: A network meta-analysis.

BACKGROUND: Administering anti-osteoporotic agents to patients perioperatively is a widely accepted approach for improving bone fusion rates and reducing the risk of complications. The best anti-osteoporotic agents for spinal fusion surgery remain unclear. AIM: To investigate the efficacy and safety of different anti-osteoporotic agents in spinal fusion surgery via network meta-analysis. METHODS: Searches were conducted in four electronic databases (PubMed, EMBASE, Web of Science, the Cochrane Library and China National Knowledge Infrastructure (CNKI) from inception to November 2022. Any studies that compared anti-osteoporotic agents vs placebo for spinal fusion surgery were included in this network meta-analysis. Outcomes included fusion rate, Oswestry disability index (ODI), and adverse events. Network meta-analysis was performed by R software with the gemtc package. RESULTS: In total, 13 randomized controlled trials were included in this network meta-analysis. Only teriparatide (OR 3.2, 95%CI: 1.4 to 7.8) was more effective than placebo in increasing the fusion rate. The surface under the cumulative ranking curve (SUCRA) of teriparatide combined with denosumab was the highest (SUCRA, 90.9%), followed by teriparatide (SUCRA, 74.0%), zoledronic acid (SUCRA, 43.7%), alendronate (SUCRA, 41.1%) and risedronate (SUCRA, 35.0%). Teriparatide (MD -15, 95%CI: -28 to -2.7) and teriparatide combined with denosumab (MD -20, 95%CI: -40 to -0.43) were more effective than placebo in decreasing the ODI. The SUCRA of teriparatide combined with denosumab was highest (SUCRA, 90.8%), followed by teriparatide (SUCRA, 74.5%), alendronate (SURCA, 52.7), risedronate (SURCA, 52.1%), zoledronic acid (SURCA, 24.2%) and placebo (SURCA, 5.6%) for ODI. The adverse events were not different between groups. CONCLUSION: This network meta-analysis suggests that teriparatide combined with denosumab and teriparatide alone significantly increase the fusion rate and decrease the ODI without increasing adverse events. Based on current evidence, teriparatide combined with denosumab or teriparatide alone is recommended to increase the fusion rate and to reduce ODI in spinal fusion patients.

The efficacy of different segments fixation for treatment thoracolumbar fractures: A Bayesian network meta-analysis.

BACKGROUND: We did this network meta-analysis to comprehensively compare the efficacy of different segments [intermediate segment (IS), short segment (SS) and long segment (LS)] in the fixation of thoracolumbar fractures. METHODS: We searched studies from inception until January 20, 2023 through PubMed, Embase, Web of Science, and the Cochrane Library. All studies comparing different segments in the fixation of thoracolumbar fractures were included in this meta-analysis. Outcomes were anterior vertebral height ratio (AVHR), sagittal Cobb angle (SCA), visual analogue scale score, and implant failure rate. This network meta-analysis was performed by R software with gemtc package. RESULTS: Finally, a total of 22 studies were finally included in this network meta-analysis. IS (WMD 2.43, 95% CrI 2.04-2.91) was more effective than SS in terms of the AVHR, and the difference was statistically significant. IS was more effective than LS in reducing SCA (WMD -2.87, 95% CrI -3.79 to -1.96) with statistically significant. Compared with SS, IS significantly reduced the SCA with statistically significant (WMD -2.52, 95% CrI -3.31 to -1.72). IS (WMD -2.87, 95% CrI -3.78 to -1.96) was more effective than LS, and the difference was statistically significant. Moreover, IS (WMD -2.52, 95% CrI -3.31 to -1.72) was more effective than SS, and the difference was statistically significant. CONCLUSION: IS was associated with a significant reduction in SCA, implant failure rate, and visual analogue scale compared to SS and LS, while having the most favorable impact on AVHR among all the treatments assessed.

Measurement of natural frequencies and mode shapes of transparent insect wings using common-path ESPI.

In this study, a common-path electronic speckle pattern interferometry system which upholds the natural property of transparency of insect's wings has been developed to measure the wings' natural frequencies and mode shapes for the first time. A novel base-exciting method was designed to enable the simultaneous application of sinusoidal and static forces to excite wings and introduce an additional phase. The moiré effect induced by the amplitude modulation was employed to accurately recognize the resonance state. Subsequently, the mode shapes were visualized by phase-shifting and real-time frame subtraction. Eight pairs of forewings from cicadas were investigated. The first three order natural frequencies of the wings are approximately 145 Hz, 272 Hz and 394 Hz, respectively, which are dispersed to prevent modal coupling. The cambered mode shapes exhibit a strongly spanwise-chordwise anisotropy flexural stiffness distribution, generally dominated by bending and twisting deformation. The details of the high-order mode shapes show that the tip exhibits distinct deformation, indicating more flexibility to cope with external impact load, and the nodal lines usually comply with the direction of the wing veins in higher modes, substantiating the fact that the veins play an important role as stiffeners of the membrane. The results are in excellent agreement with the dynamic performance of previous studies, which will potentially affect a broader community of optical measurement specialists and entomologists to enhance our understanding of time-averaged interferograms and insect flights.

Internal displacement measurements based on color fluorescent speckle pattern by multispectral stereo-DIC and refractive index correction.

Fluorescent digital image correlation (DIC) is becoming popular for measuring 3D profiles and deformations in external surfaces. However, the simultaneous monitoring of interior layers is highly challenging due to the penetrability and refraction of light using monochromatic fluorescence. We propose a color fluorescent speckle pattern (CFSP) method for measuring the internal displacement of transparent objects based on multispectral stereo-DIC and refractive index correction. During sample fabrication, fluorescent speckle patterns exciting different colors are fabricated on both the surface and interior layers of objects. A virtual color stereo-DIC system is utilized to capture the CFSP on the surface and interior layers simultaneously from two different perspectives. Different color channels are practically equivalent to synchronized monochrome vision systems, having separate CFSP in external and internal measurements. In multispectral stereo-DIC calculation, the external surface is initially reconstructed through one channel of the system even if the surface is non-planar. Based on Snell's law and the CFSP method, the internal layer is then reconstructed and corrected by establishing the geometry of the refractive stereo-DIC through another channel. The relative error of the thickness between two planar layers was proved to decrease from 33.4% to 0.7% after refractive index correction. Further experimental results validate the efficacy of this method for correcting the profile of the non-planar arc profile and determining the internal deformations of disc materials.

Semantic speckle: an auto-located speckle pattern for DIC measurement.

Digital image correlation (DIC) has been widely used in both experimental mechanics and engineering fields. The matching algorithm of the DIC method usually requires surfaces containing a random speckle pattern as a deformation information carrier. The speckle pattern plays an irreplaceable role in DIC, which has led to extensive research on it. However, most previous research had always focused on the fabrication and computational performance of the speckle, ignoring the value of intentionally defining the meaning of speckle in design. In this study, we describe a novel, to the best of our knowledge, speckle pattern named semantic speckle. It is a digital speckle composed of several different speckle patterns with similar characteristics. Based on the deep-learning method and matching algorithm, the central location of the semantic part in the overall speckle image can be obtained automatically. Through the intentional definition of the semantic part, it can be possible to calibrate the camera parameters and correct the external parameters of the DIC systems.

Effect of thrombomodulin gene polymorphisms on venous thromboembolism: An analysis of evidence involving 6,629 patients.

BACKGROUD: The association between thrombomodulin gene (THBD) c.1418 C>T polymorphisms and the risk of venous thromboembolism (VTE) is controversial. The purpose of this meta-analysis was to evaluate THBD c.1418 C>T polymorphisms and the risk of VTE. METHODS: Computer searches were performed on the CNKI, Wanfang database, VIP database, PubMed, Embase, Web of Science, and Cochrane Library databases. The retrieval time limit was from the establishment of the database to June 2022. Case-control studies and cohort studies of THBD c.1418 C>T polymorphisms associated with VTE were included. The literature was screened according to inclusion and exclusion criteria, data extraction and literature quality evaluation. Meta-analysis was performed using STATA 14.0 software. RESULTS: A total of 12 literature were included, including 2980 cases in the case group and 3649 cases in the control group. The meta-analysis results showed no significant association of the THBD c.1418 C> T polymorphisms with the occurrence of VTE (T vs C: OR = 1.17, 95%CI = 0.93-1.48; TT vs CT+CC: OR = 1.00, 95%CI = 0.75-1.33; TT+CT vs CC: OR = 1.22, 95%CI = 0.94-1.59). Subgroup analyses revealed an increased risk of VTE in Asian populations due to THBD c.1418 C>T polymorphisms (T vs C: OR = 1.48, 95%CI = 1.06-2.07; TT vs CT+CC: OR = 1.80, 95%CI = 1.13-2.85; TT+CT vs CC: OR = 1.58, 95%CI = 1.07-2.32). THBD c.1418 C>T polymorphisms increased the risk of DVT (T vs C: OR = 1.51, 95%CI = 1.24-1.85; TT vs CT+CC: OR = 1.85, 95%CI = 1.10-3.12; TT+CT vs CC: OR = 1.64, 95%CI = 1.28-2.11). THBD c.1418 C>T polymorphisms reduced the risk of VTE in non-Asian populations (TT vs CT+CC: OR = 0.66, 95%CI = 0.45-0.98). CONCLUSION: THBD c.1418 C>T polymorphisms is associated with VTE in Asian population, which may be a factor in the occurrence of VTE in Asian population. THBD c.1418 C>T polymorphisms increases the risk of DVT. Given the limitations of this meta-analysis, the conclusions require being further supported by large-scale and high-quality studies.

Point-Wise Phase Estimation Method in Fringe Projection Profilometry under Non-Sinusoidal Distortion.

In fringe projection profilometry, high-order harmonics information of distorted fringe will lead to errors in the phase estimation. In order to solve this problem, a point-wise phase estimation method based on a neural network (PWPE-NN) is proposed in this paper. The complex nonlinear mapping relationship between the gray values and the phase under non-sinusoidal distortion is constructed by using the simple neural network model. It establishes a novel implicit expression for phase solution without complicated measurement operations. Compared with the previous method of combining local image information, it can accurately calculate each phase value by point. The comparison results show that the traditional method is with periodic phase errors, while the proposed method can effectively eliminate phase errors caused by non-sinusoidal phase shifting.

Dual stereo-digital image correlation system for simultaneous measurement of overlapped wings with a polarization RGB camera and fluorescent speckle patterns.

Simultaneous monitoring of overlapped multi-wing structure by stereo-digital image correlation (stereo-DIC) may be used to quantify insect motion and deformation. We propose a dual stereo-DIC system based on multispectral imaging with a polarization RGB camera. Different fluorescent speckle patterns were fabricated on wings, which emit red and blue spectra under ultraviolet light that were imaged and separated using a polarization RGB camera and auxiliary optical splitting components. The resulting dual stereo-DIC system was validated through translation experiments with transparent sheets and reconstructed overlapped insect wings (cicadas). Dynamic measurements of the Ruban artificial flier indicate the efficacy of this approach to determining real insect flight behavior.

Tri-wavelength simultaneous ESPI for 3D micro-deformation field measurement.

Electronic speckle pattern interferometry (ESPI), a well-established technique for micro-deformation measurement, can be used to determine both in-plane and out-of-plane displacement components. Although many works in ESPI have been reported for three-dimensional (3D) displacement measurement, few works have focused on the simultaneous measurement of 3D deformation fields. Here we present an ESPI system that consists of three sub-interferometers for simultaneous measurement of all three displacement components and in-plane strain fields. A 3CCD color camera, a specially designed shifting stage, and three lasers with optimal wavelengths are used in this system. The lasers and 3CCD camera provide independent interferograms with different color signals, while the shifting stage allows the sub-interferometers to achieve simultaneous phase shifting. The results of color separation and experimental measurement demonstrate the utility of the system.

Binocular camera calibration based on timing correction.

Camera calibration is crucial to the application of binocular measurement. However, many of the calibrations are carried out manually, so shaking of the calibration plate cannot be avoided. When the cameras are not synchronized or the exposure time is inconsistent, the image acquisition time of different cameras does not correspond strictly, which will introduce inevitable calibration errors. In this paper, two consecutive frames are collected instead of one frame, and the calibration data are corrected by the image acquisition time value returned by the camera. Experiments show that the proposed method can greatly reduce the re-projection error of calibration, and achieve higher calibration accuracy and less image quantity dependence.

Experimental verification of full-field accuracy in stereo-DIC based on the ESPI method.

This paper proposes a method to merge stereo-digital image correlation (DIC) and electronic speckle pattern interferometry (ESPI) data by camera calibration. The proposed method is employed to verify the accuracy of full-field out-of-plate displacements measured by stereo-DIC in a cantilever beam test. The mean absolute error and the root mean square error (RMSE) of the full-field displacement measured by four-megapixel cameras are 0.849 µm and 1.08 µm at 60 mm field of view, respectively, and the RMSE of the central area is 0.615 µm. The errors are not uniformly distributed because of the imperfect calibration. When the lenses are changed and the field of view reaches 120 mm, the RMSE is 1.48 µm with uniform distribution. These accuracies could be traced back to the laser wavelength to confirm the stereo-DIC data. The proposed method can be used not only to verify the full-field measurement accuracy of DIC but also to determine the rigid-body displacement for ESPI with a high-precision stereo-DIC. Thus, the displacement vector can be obtained. Furthermore, it can unify the coordinate of multiple ESPI systems to achieve a large range of high-precision three-dimensional deformation measurements.

Efficient ESPI method to identify vibration characteristics of transparent films.

Electronic speckle pattern interferometry (ESPI) has been frequently used to study vibration characteristics of engineering structures. However, efficient ESPI methods for identifying the vibration characteristics of transparent membranes are rarely reported. A convenient approach to determine the resonant frequencies and the vibration modes of transparent films is proposed. A quasi-common-path ESPI (QCP-ESPI) is developed to detect out-of-plane deformation and visualize vibrational modes of a vibrating transparent diaphragm. An amplitude-modulation-based method is proposed for quick and accurate determining resonance of oscillating objects during frequency scanning. Experimental performances demonstrate the feasibility and efficiency of the QCP-ESPI and amplitude-modulation-based methods for determining the resonant frequency and vibration modes of transparent objects.

[Effect and Involved Mechanism of RSL3-induced Ferroptosis in Acute Leukemia Cells MOLM13 and Drug-resistant Cell Lines].

OBJECTIVE: To investigate the effect and involved mechanism of RSL3 on ferroptosis action in acute leukemia cells MOLM13 and its drug-resistant cells. METHODS: After MOLM13 treated with RSL3, CCK-8 assay was performed to detect cell viability, flow cytometry was used to detect the reactive oxygen species (ROS) level of the cells, RT-qPCR and Western blot were used to detect the expression of glutathione peroxidase 4 (GPX4). After MOLM13/IDA and MOLM13/Ara-C, the drug-resistant cell lines were constructed, the ferroptosis induced by RSL3 was observed. Bone marrow samples were collected from patients with acute monocytic leukemia. RT-qPCR and Western blot were performed to detect the expression of related genes and proteins involved in ferroptosis pathway. RESULTS: RSL3 significantly inhibited the cell viability of MOLM13 and increased the intracellular ROS level, which were partially reversed by ferrostatin-1. The mRNA and protein expression of GPX4 decreased in MOLM13 treated with RSL3. RSL3 inhibited the viability of MOLM13/IDA and MOLM13/Ara-C cells more strongly than that of non-drug resistant cells, also increased the intracellular ROS level . The cytotoxic effects were partially reversed by ferrostatin-1. The mRNA and protein expressions of GPX4 in MOLM13/IDA and MOLM13/Ara-C cells were higher than those in non-drug resistant cells. The mRNA and protein levels of GPX4 in bone marrow of relapsed/refractory acute mononuclear leukemia patients were higher than those of ordinary acute mononuclear leukemia patients. CONCLUSION: RSL3 can induce non-drug resistant cells MOLM13 ferroptosis by inhibiting GPX4 activity. MOLM13/IDA and MOLM13/Ara-C are more sensitive to RSL3 compared with non-drug resistant cells MOLM13, which may be caused by the differences in GPX4 expression. The expressions of GPX4 mRNA and protein in relapsed/refractory acute mononuclear leukemia are higher than those in ordinary acute mononuclear leukemia.

Optimization of multiscale digital speckle patterns for multiscale deformation measurement using stereo-digital image correlation.

Simultaneous monitoring of multiple fields of view (FOVs) by multiscale stereo-digital image correlation (stereo-DIC) can quantify the deformation of a material when localized phenomena occur within a larger FOV or moving object. In multiscale deformation measurement via stereo-DIC, optimization of the digital speckle patterns (DSPs) is essential to achieve high accuracy and efficiency. This work optimizes and fabricates multispectral DSPs used for multiple scales. First, an optimization of the DSP for two FOVs is achieved using both spatial modulation and specified spectra. A spatially modulated DSP is compared with two spectral DSPs achieved by visible and ultraviolet-excited blue light. Then, a spatially modulated visible DSP fabricated by an ultraviolet printer overlaid with an ultraviolet-excited blue DSP fabricated by a photosensitive seal is designed for multiscale stereo-DIC measurements of three FOVs. Experiments were performed to illustrate the functionality and utility of this multiscale DSP. Such experimental analyses can supply adequate full-field data to validate localized or kinetic mechanical behavior.

Low-dose decitabine plus venetoclax is safe and effective as post-transplant maintenance therapy for high-risk acute myeloid leukemia and myelodysplastic syndrome.

Acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) are usually associated with poor outcomes, especially in high-risk AML/MDS. Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only curative option for patients suffering from high-risk AML/MDS. However, many patients relapse after allo-HSCT. Novel therapy to prevent relapse is urgently needed. Both the BCL-2 inhibitor venetoclax (VEN) and the hypomethylating agent decitabine (DEC) possess significant antitumor activity effects against AML/MDS. Administration of DEC has been shown to ameliorate graft-versus-host disease (GVHD) and boost the graft-versus-leukemia (GVL) effect post-transplantation. We therefore conducted a prospective study (ChiCTR1900025374) to examine the tolerability and efficacy of a maintenance therapy of low-dose decitabine (LDEC) plus VEN to prevent relapse after allo-HSCT for high-risk AML/MDS patients. Twenty patients with high-risk AML (n = 17) or high-risk MDS (n = 3) post-transplantation were recruited. Approximately day 100 post-transplantation, all patients received LDEC (15 mg/m2 for 3 d) followed by VEN (200 mg) on d 1-21. The cycle interval was 2 mo, and there was 10 cycles. The primary end points of this study were rates of overall survival (OS) and event-free survival (EFS). The secondary endpoints included adverse events (AEs), cumulative incidence of relapse (CIR), nonrelapse mortality (NRM), incidences of acute GVHD (aGVHD) and chronic GVHD (cGVHD), and incidences of viral infection after allo-HSCT. Survival outcomes were assessed using Kaplan-Meier analysis. The median follow-up was 598 (149-1072) d. Two patients relapsed, 1 died, and 1 is still alive after the second transplant. The 2-y OS and EFS rates were 85.2% and 84.7%, respectively. The median 2-y EFS time was 525 (149-1072) d, and 17 patients still had EFS and were alive at the time of this writing. The most common AEs were neutropenia, anemia, thrombocytopenia, neutropenic fever, and fatigue. Grade 2 or 3 AEs were observed in 35% (7/20) and 20% (4/20) of the patients, respectively. No grade >3 AEs were observed. aGVHD (any grade) and cGVHD (limited or extensive) occurred in 55% and 20% of patients, respectively. We conclude that LDEC + VEN can be administered safely after allo-HSCT with no evidence of an increased incidence of GVHD, and this combination decreases the relapse rate in high-risk AML/MDS patients. This novel maintenance therapy may be a promising way to prevent relapse in high-risk AML/MDS patients.

Refractive three-dimensional reconstruction for underwater stereo digital image correlation.

Measuring the three-dimensional (3D) deformation of submerged objects through different media with the stereo digital image correlation (stereo-DIC) method involves the refractive optical imaging problem where the non-linear transmission of light is induced by a change of medium density. The problem invalidates the underlying single viewpoint assumption of the perspective model in regular stereo-DIC, thereby resulting in erroneous measurements of 3D shape and deformation. In this work, we propose a refractive stereo-DIC method that overcomes the problem by considering light refraction in 3D reconstruction. We formulate a full refractive reconstruction geometry description based on Snell's law of flat refraction and the regular triangulation. This allows the true shape to be effectively reconstructed by tracing and establishing the refracted ray-paths based on the regular 3D reconstruction, without reformulating the camera model and image formation. The refractive stereo-DIC is finally established by integrating the refractive 3D reconstruction into the regular DIC framework for measuring accurate 3D shape and deformation of submerged objects. We experiment the proposed approach with underwater 3D shape and deformation measurements. Both results prove its feasibility and correctness, further heralding our approach as a flexible solution that could readily extend the stereo-DIC to fluid-immersed 3D deformation characterization.

Multifunctional ultrathin reflective metasurface via polarization-decoupled phase for arbitrary circularly or elliptically polarized waves.

Metasurface offers a promising platform in the design of multifunctional devices owing to its unique ability for EMWs manipulation. However, wave-manipulation capabilities for metasurfaces face challenges in manipulating orthogonal EMWs with arbitrary circularly or elliptically polarized EMWs in the microwave region. Herein, single-layer reflective metasurfaces are proposed for independent manipulation of an arbitrary set of orthogonal circularly or elliptically polarized EMWs via polarization-decoupled phase. Taking advantage of single-layer anisotropic meta-atoms, the proposed metasurface can act as a tandem phase modulator, which introduces polarization-decoupled phase profiles for arbitrary circularly and elliptically polarized EMWs based on the Jones matrix. In this way, the proposed metasurface can distinguish a set of orthogonal EMWs with circular or elliptical polarization states and impose arbitrary phase profiles on them independently and simultaneously. For proof-of-concept, bifunctional metasurfaces operating in the microwave region are presented for independent manipulation of three different sets of orthogonal circularly or elliptically polarized EMWs. They create dual independent channels associated with a pair of orthogonal polarization states, performing functions including polarization beam splitting and orbital angular momentum (OAM) multiplexing. Measured and simulated results show a good agreement, confirming that the proposed single-layer reflective metasurfaces are efficient devices that enable meta-devices to independently control arbitrary circular and elliptical polarized EMWs, achieving arbitrary functionalities.