RESUMEN
A strictly anaerobic, Gram-stain-negative rod-shaped bacterium, designated A1-XYC3T, was isolated from the faeces of an alpaca (Lama pacos). On the basis of the results of a comparative 16S rRNA gene sequence analysis, the isolate was assigned to the genus Clostridium with the highest sequence similarities to Clostridium magnum DSM 2767T (96.8â%), Clostridium carboxidivorans P7T (96.3â%) and Clostridium aciditolerans JW/YJL-B3T (96.1â%). The average nucleotide identity between A1-XYC3T, C. magnum, C. carboxidivorans and C. aciditolerans was 77.4, 76.1 and 76.6ââ%, respectively. The predominant components of the cellular fatty acids of A1-XYC3T were C14â:â0, C16â:â0 and summed feature 10, containing C18:0/C17:0 cyclo. The DNA G+C content was 32.4 mol%. On the basis of biochemical, phylogenetic, genotypic and chemotaxonomic criteria, this isolate represents a novel species within Clostridium sensu stricto for which the name Clostridium tanneri sp. nov. is proposed. The type strain of this species is strain A1-XYC3T (=CCM 9376T=NRRL B-65691T).
Asunto(s)
Técnicas de Tipificación Bacteriana , Composición de Base , Camélidos del Nuevo Mundo , Clostridium , ADN Bacteriano , Ácidos Grasos , Heces , Filogenia , ARN Ribosómico 16S , Análisis de Secuencia de ADN , Camélidos del Nuevo Mundo/microbiología , Heces/microbiología , ARN Ribosómico 16S/genética , Animales , Clostridium/genética , Clostridium/clasificación , Clostridium/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/análisis , Datos de Secuencia MolecularRESUMEN
Two strictly anaerobic, Gram-stain-negative rod-shaped bacterial isolates, A2-P53T and A1-P5, were isolated from an enrichment of fecal material from two alpacas (Vicugna pacos). Based on a comparative 16S rRNA gene sequence analysis, the isolates were assigned to the genus Bacteroides with the highest sequence similarities to Bacteroides koreensis YS-aM39T (A2- P53T 97.7% and A1-P5 97.9%). Additionally, the average nucleotide identity and digital DNA-DNA hybridization values between these isolates and their closest relatives within Bacteroides were less than 92.1% and 49.1%, respectively. The average nucleotide identity between isolates A2-P53T and A1-P5 was 99.9%. The predominant cellular fatty acid for isolates A2-P53T and A1-P5 was C15:0 antesio. The G+C % content of the isolates was 41.7%. Based on biochemical, phylogenetic, genotypic, and chemotaxonomic criteria, these isolates A2-P53T and A1-P5 represent two individual strains of a novel species within the genus Bacteroides for which the name Bacteroides vicugnae sp. nov. is proposed. The type strain of this species is strain A2-P53T (CCUG 77273T=CCM 9377T=NRRL B-65693T).