RESUMEN
PURPOSE: Vitreo-macular pathology may be influenced by vitreo-papillary adhesion (VPA). Optical coherence tomography/scanning laser ophthalmoscopy (OCT/SLO) was used to identify VPA in full-thickness macular hole, lamellar hole, and macular pucker (MP). METHODS: Ultrasonography and OCT/SLO were performed in 55 subjects: 16 with macular hole, 11 with lamellar hole, and 28 with MP. The main outcome measures were the presence of posterior vitreous detachment by ultrasound, and the findings of VPA and intraretinal cysts by OCT/SLO. RESULTS: Posterior vitreous detachment was detected by ultrasound in 26/28 (92.9%) eyes with MP, 6/11 (54.5%) eyes with lamellar hole (P < 0.05), and 4/16 (25%) eyes with macular hole (P < 0.00001). Optical coherence tomography/scanning laser ophthalmoscopy detected VPA in 14/16 (87.5%) macular hole eyes, 4/11 (36.4%) lamellar hole eyes (P < 0.05), and 5/28 (17.9%) MP eyes (P < 0.00005). Intraretinal cysts were present in 4/5 (80%) MP eyes with VPA but only 1/23 (4.3%) MP eyes without VPA (P < 0.005). CONCLUSION: Vitreo-papillary adhesion was significantly more common in full-thickness macular hole than lamellar hole or MP. When present in MP, VPA was frequently associated with intraretinal cysts. Hence, VPA may have an important influence on the vectors of force at the vitreo-retinal interface inducing holes and cysts.
Asunto(s)
Membrana Epirretinal/diagnóstico , Disco Óptico/patología , Enfermedades del Nervio Óptico/diagnóstico , Perforaciones de la Retina/diagnóstico , Cuerpo Vítreo/patología , Desprendimiento del Vítreo/diagnóstico , Adulto , Anciano , Anciano de 80 o más Años , Quistes/diagnóstico , Femenino , Humanos , Masculino , Microscopía Acústica , Persona de Mediana Edad , Oftalmoscopía , Enfermedades de la Retina/diagnóstico , Adherencias Tisulares , Tomografía de Coherencia ÓpticaRESUMEN
We are investigating the redox chemistry of wild-type (WT) and mutant (1-12G) cytochrome P450 BM3. Absorption spectra in solution feature the Fe(III) Soret at 418 nm for WT and a split Soret for 1-12G at 390 and 418 nm. Voltammetry of the proteins within DDAPSS films on the surface of carbon electrodes reveal nearly identical Fe(III/II) potentials (approximately -200 mV vs Ag/AgCl), but significant differences in k degrees , 250 vs 30 s(-)(1), and Fe(III/II)-CO potentials, -140 vs -115 mV, for WT vs 1-12G. Catalytic reduction of dioxygen by the proteins on rotating-disk electrodes was analyzed using Levich and Koutecky-Levich treatments. The data reveal 1-12G n and k(obs) values that are, respectively, 1.7 and 0.07 times those of WT, suggesting that the two proteins differ strikingly in their reactions with dioxygen.