Visible photodegradation of ibuprofen and 2,4-D in simulated waste water using sustainable metal free-hybrids based on carbon nitride and biochar.

Rational designing of metal-free carbon nitride based photocatalysts can lead to an excellent optical response and a higher photocatalytic activity driven by visible and solar light. This combines green photocatalytic technology with greener materials prepared by facile approaches for environmental remediation. Herein we report utilization of star photocatalyst g-C3N4 (CN) to form highly efficient hetero-assemblies along with acidified g-C3N4 (ACN), polyaniline (PANI), reduced graphene oxide (RGO) and biochar. By use of these organic semiconductors we synthesize g-C3N4/ACN/RGO@Biochar (GARB), g-C3N4/PANI/RGO@Biochar (GPRB) and ACN/PANI/RGO@Biochar (APRB) nano-assemblies with different optical response and band edge positions for a better charge flow and reduced recombination of carriers. These synthesized catalysts were used for visible light powered degradation of 2,4-Dichlorophenoxy acetic acid (2,4-D) and ibuprofen (IBN). APRB performs the best and degrades 99.7% and 98.4% of 2,4-D and IBN (20 mg L-1) under Xe lamp exposure in 50 min and retention of high activity in natural sunlight. Optical analysis, photoelectrochemical response and radical quenching studies show both hydroxyl and superoxide radical anions as major reactive species and a Z-scheme photocatalytic mechanism. RGO acts as an electron mediator and protects higher positioned bands of PANI and ACN in APRB for a remarkable photocatalytic activity for a metal free material. The degradation pathway was analyzed by LC-MS analysis and 42% and 40% total organic carbon was removed in 2 h for 2,4-D and IBN degradation respectively. The toxicity of degraded products was analyzed by analyzing viability of human peripheral blood cells with retaining of 99.1% cells.

Pectin-guar gum-zinc oxide nanocomposite enhances human lymphocytes cytotoxicity towards lung and breast carcinomas.

Pectin-guar gum-zinc oxide (PEC-GG-ZnO) nanocomposite was prepared by precipitation technique. The composite was characterized by using FT-IR, XRD, HRTEM, SAED, EDS, and SEM. TEM images showed the hexagonal shape of nanocomposite with the size range of 50-70 nm. Further, PEC-GG-ZnO was used as an immunomodulator for the first time to improve the cancer cells killing capabilities of human peripheral-blood lymphocytes (PBL). The lymphocyte proliferation assay proved the immunostimulatory property of the PEC-GG-ZnO which increased with the increase in concentration (25 µg/ml to 200 µg/ml). ELISA detection confirmed a significant increase in the release of IFN-γ, IL-2 and TNF-α cytokines and flow cytometry analysis revealed enhanced expression of CD3, CD8, and CD56 after treating PBL with PEC-GG-ZnO as compared to PEC and GG treatment. Moreover, we also found that nanocomposite pretreated human PBL displayed enhanced cytotoxicity towards lung (A549) and breast carcinoma (MCF-7) cells as compared to untreated PBL. The microcytotoxicity assay also demonstrated that with increase in effector: target ratios from 2.5:1 to 20:1, there was an increase in the cancer cell death. Taken together, the current data corroborates the immunostimulatory activities of PEC-GG-ZnO, a novel nanocomposite, hence it can serve as a promising cancer therapeutic agent.