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Little is known about Native American adaptations to blood-sucking arthropods prior to and following European contact. Multiple accounts starting in the 16th century suggest that rancid animal grease was employed by Gulf Coast indigenes as a mosquito repellent. Although many Native American ethnobotanical remedies for biting insects have been recorded, the use of animal products for this purpose is not well documented. Moreover, few traditional Native American mosquito repellents have been examined using controlled laboratory methods for repellency testing. In this study, we tested the repellent efficacy of fats derived from alligator, bear, cod, and shark that were aged to various stages of rancidity. Using yellow fever mosquitoes, (Aedes aegypti), we performed an arm-in-cage assay to measure the complete protection times resulted from these fats, when applied to human skin. We used a Y-tube olfactometer assay to evaluate long-distance repellency and tested tick-repellency in a crawling assay. Our results suggest that rancid animal fats from cod, bear, and alligator are potent albeit short-lived mosquito repellents. We found that both rancid and fresh fats do not repel ticks. Our findings show the validity of traditional ethnozoological knowledge of Native American people and support aspects of the ethnohistorical record.
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Repelentes de Insectos , Animales , Humanos , Aedes/efectos de los fármacos , Grasas , Indígenas Norteamericanos , Repelentes de Insectos/farmacologíaRESUMEN
Natural rubber produced in stems of the guayule plant (Parthenium argentatum) is susceptible to post-harvest degradation from microbial or thermo-oxidative processes, especially once stems are chipped. As a result, the time from harvest to extraction must be minimized to recover high quality rubber, especially in warm summer months. Tocopherols are natural antioxidants produced in plants through the shikimate and methyl-erythtiol-4-phosphate (MEP) pathways. We hypothesized that increased in vivo guayule tocopherol content might protect rubber from post-harvest degradation, and/or allow reduced use of chemical antioxidants during the extraction process. With the objective of enhancing tocopherol content in guayule, we overexpressed four Arabidopsis thaliana tocopherol pathway genes in AZ-2 guayule via Agrobacterium-mediated transformation. Tocopherol content was increased in leaf and stem tissues of most transgenic lines, and some improvement in thermo-oxidative stability was observed. Overexpression of the four tocopherol biosynthesis enzymes, however, altered other isoprenoid pathways resulting in reduced rubber, resin and argentatins content in guayule stems. The latter molecules are mainly synthesized from precursors derived from the mevalonate (MVA) pathway. Our results suggest the existence of crosstalk between the MEP and MVA pathways in guayule and the possibility that carbon metabolism through the MEP pathway impacts rubber biosynthesis.
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Asteraceae , Hojas de la Planta , Tallos de la Planta , Tocoferoles , Tocoferoles/metabolismo , Tocoferoles/química , Hojas de la Planta/metabolismo , Hojas de la Planta/química , Tallos de la Planta/metabolismo , Tallos de la Planta/química , Tallos de la Planta/genética , Asteraceae/metabolismo , Asteraceae/química , Asteraceae/genética , Goma/metabolismo , Goma/química , Arabidopsis/metabolismo , Arabidopsis/genética , Arabidopsis/química , Resinas de Plantas/metabolismo , Resinas de Plantas/químicaRESUMEN
Jujube, commonly known as the Chinese date, is a nutritious fruit with medicinal importance. Fresh jujube fruits have a shelf life of about ten days in ambient conditions that can be extended by drying. However, nutrition preservation varies with the drying method and parameters selected. We studied total phenolic content (TPC), proanthocyanidins (PA), vitamin C, cyclic adenosine monophosphate (cAMP), and antioxidant activities in jujube fruits dried with freeze-drying (FD), convective oven drying (OD) at 50 °C, 60 °C, and 75 °C, and sun drying (SD) with FD as a control. The cultivars used for this study were 'Capri' and 'Xiang' from Las Cruces in 2019, and 'Sugarcane', 'Lang', and 'Sherwood' from Las Cruces and Los Lunas, New Mexico, in 2020. Freeze-drying had the highest of all nutrient components tested, the best estimates of mature jujube fruits' nutrient contents. Compared with FD, the majority of PA (96-99%) and vitamin C (90-93%) was lost during SD or OD processes. The retention rates of antioxidant activities: DPPH and FRAP were higher in OD at 50/60 °C than SD. SD retained a higher cAMP level than OD at 50/60 °C in both years. The increase in oven drying temperature from 60 °C to 75 °C significantly decreased TPC, PA, antioxidant activities, and cAMP.
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Cases of mosquito- and tick-borne diseases are rising worldwide. Repellent products can protect individual users from being infected by such diseases. In a previous study, we identified five essential oils that display long-distance mosquito repellency using a Y-tube olfactometer assay. In the current study, the contact repellent efficacy of 20 active ingredients from the Environmental Protection Agency's (EPA) Minimum Risk Pesticides list were tested using Aedes aegypti and Ixodes scapularis. We utilized an arm-in-cage assay to measure complete protection time from mosquito bites for these active ingredients. To measure tick repellency, we used an EPA-recommended procedure to measure the complete protection time from tick crossings. We found that of the 20 ingredients tested, 10% v/v lotion emulsions with clove oil or cinnamon oil provided the longest protection from both mosquito bites and tick crossings. We conclude that in a 10% v/v emulsion, specific active ingredients from the EPA Minimum Risk Pesticides list can provide complete protection from mosquito bites and tick crossings for longer than one hour.
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Aedes , Mordeduras y Picaduras de Insectos , Repelentes de Insectos , Ixodes , Aceites Volátiles , Animales , Humanos , Aceites Volátiles/farmacología , Repelentes de Insectos/farmacologíaRESUMEN
(-)-Cannabidiol [(-)-CBD] has recently gained prominence as a treatment for neuro-inflammation and other neurodegenerative disorders; interest is also developing in its synthetic enantiomer, (+)-CBD, which has a higher affinity to CB1 / CB2 receptors than the natural stereoisomer. We have developed an inexpensive, stereoselective route to access ent-CBD derivatives using (+)-carvone as a starting material. In addition to (+)-CBD, we report the first syntheses of (+)-cannabidivarin, (+)-cannabidiphorol as well as C-6 / C-8 homologues.
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The first asymmetric total synthesis of C(9)-S-(+)-taumycin A is now reported using an approach that targeted both C(9) diastereomers concurrently. To facilitate this work, we called upon the symmetrical nature of a C(5)-C(13) side-chain intermediate and exploited orthogonal protecting groups as a tactic to access both stereoisomers from a single chiral, nonracemic intermediate. In addition to our successful approach, several minor detours that helped refine our strategy and a detailed analysis of 1H NMR data will be discussed. Select compounds included in this work were screened against the NCI60 cell line panel and displayed modest growth inhibition activity.
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Depsipéptidos , EstereoisomerismoRESUMEN
The fat body is considered the insect analog of vertebrate liver and fat tissue. In mosquitoes, a blood meal triggers a series of processes in the fat body that culminate in vitellogenesis, the process of yolk formation. Lipids are stored in the fat body in specialized organelles called lipid droplets that change in size depending on the nutritional and metabolic status of the insect. We surveyed lipid droplets in female Aedes aegypti fat body during a reproductive cycle using confocal microscopy and analyzed the dynamic changes in the fat body lipidome during this process using LC/MS. We found that lipid droplets underwent dynamic changes in volume after the mosquito took a blood meal. The lipid composition found in the fat body is quite complex with 117 distinct lipids that fall into 19 classes and sublcasses. Our results demonstrate that the lipid composition of the fat body is complex as most lipid classes underwent significant changes over the course of the vitellogenic cycle. This study lays the foundation for identifying unknown biochemical pathways active in the mosquito fat body, that are high-value targets for the development of novel mosquito control strategies.
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Guayule (Parthenium argentatum), a shrub native to the arid region of the U.S. southwest and Mexico belonging to the Asteraceae family, is a source of high quality, hypoallergenic natural rubber with applications in pharmaceutical, tire, and food industries. Production of rubber results in a substantial amount of resin-containing residues which contain a wide variety of secondary metabolites (sesquiterpene esters, triterpene alcohols, fatty acids, etc.). In order to enhance the economic viability of guayule as an industrial crop, value-added use of the residues is needed and has the potential to reduce gross rubber production costs. The main objective of this research is the characterization of guayule resin using rapid and accurate analytical techniques to identify compounds of potential commercial value. Guayule resin is inherently complex and includes many high-molecular-weight and non-volatile compounds that are not easy to observe using traditional chromatographic techniques. The combination of two mass spectroscopy techniques: gas chromatography mass spectroscopy (GC-MS) and high-resolution Fourier transform ion cyclotron resonance mass spectroscopy (FT-ICR MS), were used to characterize the composition of the extracted resin from guayule (Parthenium argentatum). FT-ICR MS was used to characterize hundreds of compounds with over a wide range of molecular weights and degrees of aromaticity at higher levels of mass accuracy than other forms of mass spectrometry. GC-MS was used to identify volatile compounds like mono- and sesquiterpene compounds.
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Carotenoids are lipophilic pigments found in plants and algae, as well as some bacteria, archaea, and fungi that serve two functions-(1) as light harvesting molecules-primary carotenoids, and (2) as antioxidants, acting against reactive oxygen speciesâ»secondary carotenoids. Because of their strong antioxidant properties, they are also valuable for the development of anti-aging and photo-protective cosmetic applications. Of particular interest is the carotenoid phytoene, for its colorless and UV absorption characteristics. In this study, we targeted a reduction of phytoene desaturase (PDS) activity with the pigment-inhibiting herbicide 1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]pyridin-4-one (fluridone), which leads to the over-accumulation of phytoene in the recently characterized microalgal strain Chlorococcum sp. (UTEX B 3056). After post-incubation with fluridone, phytoene levels were measured at ~33 ug/mg cell tissue, as opposed to non-detectable levels in control cultures. Hence, the novel microalga Chlorococcum sp. is a viable candidate for the production of the high-value carotenoid phytoene and subsequent applications in cosmeceuticals, as well as more obvious nutraceutical and pharmaceutical applications.
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Carotenoides/metabolismo , Chlorophyceae/efectos de los fármacos , Chlorophyceae/metabolismo , Piridonas/farmacología , Carotenoides/biosíntesis , Ácidos Grasos/metabolismo , Oxidorreductasas/antagonistas & inhibidores , Oxidorreductasas/metabolismoRESUMEN
Enhanced reactivity of aqueous ozone (O3) with hydroxypropyl-ß-cyclodextrin (HPßCD) and its impact on relative reactivity of O3 with contaminants were evaluated herein. Oxidation kinetics of 1,4-dioxane, trichloroethylene (TCE), and 1,1,1-trichloroethane (TCA) using O3 in single and multiple contaminant systems, with and without HPßCD, were quantified. 1,4-Dioxane decay rate constants for O3 in the presence of HPßCD increased compared to those without HPßCD. Density functional theory molecular modeling confirmed that formation of ternary complexes with HPßCD, O3, and contaminant increased reactivity by increasing reactant proximity and through additional reactivity within the HPßCD cavity. In the presence of chlorinated co-contaminants, the oxidation rate constant of 1,4-dioxane was enhanced. Use of HPßCD enabled O3 reactivity within the HPßCD cavity and enhanced 1,4-dioxane treatment rates without inhibition in the presence of TCE, TCA, and radical scavengers including NaCl and bicarbonate. Micro-environmental chemistry within HPßCD inclusion cavities mediated contaminant oxidation reactions with increased reaction specificity.
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2-Hidroxipropil-beta-Ciclodextrina/farmacología , Dioxanos/farmacología , Ozono , Purificación del Agua/métodos , Agua Subterránea/química , Cinética , Oxidación-Reducción , Ozono/química , Tricloroetanos/farmacología , Tricloroetileno/farmacología , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/químicaRESUMEN
A comprehensive compartment model is presented for PFAS retention that incorporates all potential processes relevant for transport in source zones. Miscible-displacement experiments were conducted to investigate separately the impact of adsorption at the air-water and decane-water interfaces on PFAS retention and transport. Two porous media were used, a quartz sand and a soil, and perfluorooctanesulfonic acid (PFOS) was used as the model PFAS. The breakthrough curves for transport under water-unsaturated conditions were shifted noticeably rightward (delayed arrival) compared to the breakthrough curves for saturated conditions, indicating greater retardation due to adsorption at the air-water or decane-water interface. The retardation factor was 7 for PFOS transport in the sand for the air-water system, compared to 1.8 for saturated conditions. PFOS retardation factors for transport in the soil were 7.3 and 3.6 for unsaturated (air-water) vs saturated conditions. Air-water interfacial adsorption is a significant source of retention for PFOS in these two systems, contributing more than 80% of total retention for the sand and 32% for the soil. For the experiments conducted with decane residual emplaced within the sand, adsorption at the decane-water interface contributed more than 70% to total retention for PFOS transport. Methods to determine or estimate key distribution variables are presented for parameterization of the model. Predicted retardation factors were similar to the measured values, indicating that the conceptual model provided adequate representation of the relevant retention processes and that the parameter estimation methods produced reasonable values. The results of this work indicate that adsorption by fluid-fluid interfaces in variably saturated porous media can be a significant retention process for PFAS that should be considered when characterizing their transport and fate behavior in source zones.
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Fluorocarburos , Adsorción , Porosidad , Dióxido de Silicio , AguaRESUMEN
In humans, dietary polyunsaturated fatty acids (PUFAs) are involved in therapeutic processes such as prevention and treatment of cardiovascular diseases, neuropsychiatric disorders, and dementia. We examined the physiology, PUFA accumulation and glycerol lipid biosynthesis in the marine microalga Nannochloropsis salina in response to constant suboptimal temperature (<20 °C). As expected, N. salina exhibited significantly reduced growth rate and photosynthetic activity compared to optimal cultivation temperature. Total fatty acid contents were not significantly elevated at reduced temperatures. Cultures grown at 5 °C had the highest quantity of eicosapentanoic acid (EPA) (C20:5n3) and the lowest growth rate. Additionally, we monitored broadband lipid composition to model the occurrence of metabolic alteration and remodeling for various lipid pools. We focused on triacylglycerol (TAG) with elevated PUFA content. TAGs with EPA at all three acyl positions were higher at a cultivation temperature of 15 °C. Furthermore, monogalactosyldiacylglycerol and digalactosyldiacylglycerol, which are polar lipids associated with chloroplast membranes, decreased with reduced cultivation temperatures. Moreover, gene expression analysis of key genes involved in Kennedy pathway for de novo TAG biosynthesis revealed bimodal variations in transcript level amongst the temperature treatments. Collectively, these results show that Nannochloropsis salina is a promising source of PUFA containing lipids.
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Aclimatación/fisiología , Vías Biosintéticas/genética , Microalgas/fisiología , Estramenopilos/fisiología , Triglicéridos/biosíntesis , Frío , Ácidos Grasos Insaturados/química , Ácidos Grasos Insaturados/metabolismo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica/fisiología , Metabolismo de los Lípidos/fisiología , Metaboloma/fisiología , Aguas Salinas , Triglicéridos/químicaRESUMEN
The use of trimethylsilyl trifluoromethanesulfonate as a mild means to unite epoxy-carvone silyl ethers with anisole derivatives to yield products that are structurally similar to the CBD scaffold is reported. Importantly, unlike related methods, this process can utilize both epoxy-carvone diastereomers and does not require the use of air/moisture-sensitive organometallic reagents. Several examples of aryl nucleophiles as well as mechanistic insight based on in silico computational analysis are presented.
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Public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. All target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. Vertebrate blood, however, is costly, with a short shelf life. To overcome these constraints, we have developed SkitoSnack, an artificial blood meal replacement for the mosquito Aedes aegypti, the vector of dengue, Zika and chikungunya virus. SkitoSnack contains bovine serum albumin and hemoglobin as protein source as well as egg yolk and a bicarbonate buffer. SkitoSnack-raised females had comparable life history traits as blood-raised females. Mosquitoes reared from SkitoSnack-fed females had similar levels of infection and dissemination when orally challenged with dengue virus type 2 (DENV-2) and significantly lower infection with DENV-4. When SkitoSnack was used as a vehicle for DENV-2 delivery, blood-raised and SkitoSnack-raised females were equally susceptible. The midgut microbiota differed significantly between mosquitoes fed on SkitoSnack and mosquitoes fed on blood. By rearing 20 generations of Aedes exclusively on SkitoSnack, we have proven that this artificial diet can replace blood in mosquito mass rearing.
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Aedes/fisiología , Sustitutos Sanguíneos , Microbioma Gastrointestinal/fisiología , Aedes/metabolismo , Animales , Femenino , Microbioma Gastrointestinal/genética , Hierro/metabolismoRESUMEN
We report the co-liquefaction performance of unicellular, red alga Cyanidioschyzon merolae and Galdieria sulphuraria under sub-critical water conditions within a stainless-steel batch reactor under different temperatures (150-300°C), residence time (15-60min), and Cyanidioschyzon merolae to Galdieria sulphuraria mass loading (0-100%). Individual liquefaction of C. merolae and G. sulphuraria at 300°C achieved maximum biocrude oil yield of 18.9 and 14.0%, respectively. The yield of biocrude oil increased to 25.5%, suggesting a positive synergistic effect during the co-liquefaction of 80-20mass loading of C. merolae to G. sulphuraria. The biocrude oils were analyzed by FT-ICR MS which showed that co-liquefaction did not significantly affect the distribution of product compounds compared to individual oils. The co-liquefied biocrude and biochar have a higher-heating-value of 35.28 and 7.96MJ/kg. Ultimate and proximate analysis were performed on algae biomass, biocrude and biochar.
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Microalgas , Biomasa , Aceites , Temperatura , AguaRESUMEN
BACKGROUND: Anthocyanin-rich blue corn is an emerging specialty crop in the USA. The antioxidant properties of blue corn offer health benefits in the human diet. The objectives of this study were to identify, characterize and quantify the anthocyanins from blue corn. Hypotheses tested were that total anthocyanin content was similar among southwestern US accessions and that it would vary across locations. It was also examined whether different anthocyanin components were unique to certain genotypes. RESULTS: Across all locations and accessions, an average of 0.43 g kg(-1) total anthocyanin content (TAC) was observed. Accessions Santa Clara Blue and Ohio Blue displayed the highest TAC. The TAC of accession Flor del Rio was lower by nearly a factor of six. A total of five anthocyanin components were identified. Cyanidin 3-glucoside was the most abundant, followed by pelargonidin and peonidin 3-glucoside. Succinyl and disuccinyl glycosidic forms of cyanidin were also identified. Cyanidin 3-disuccinylglucoside was newly identified as a novel form of anthocyanin. CONCLUSION: Quantitative and qualitative anthocyanin expression was determined to be relatively stable across multiple southwestern environments. Increased expression of red and purple pigmentation in accession Flor del Rio appeared to be associated more with reduced TAC and cyanidin 3-glucoside than with elevated pelargonidin per se. A previously unreported anthocyanin component in blue corn, cyanidin 3-disuccinylglucoside, is present in southwestern landraces. © 2016 Society of Chemical Industry.
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Antocianinas/análisis , Productos Agrícolas/química , Calidad de los Alimentos , Alimentos Funcionales/análisis , Pigmentos Biológicos/biosíntesis , Semillas/química , Zea mays/química , Altitud , Antocianinas/biosíntesis , Antocianinas/metabolismo , Antioxidantes/análisis , Antioxidantes/metabolismo , Producción de Cultivos , Productos Agrícolas/crecimiento & desarrollo , Productos Agrícolas/metabolismo , Glucósidos/análisis , Glucósidos/biosíntesis , Humanos , Fitomejoramiento , Análisis de Componente Principal , Semillas/crecimiento & desarrollo , Semillas/metabolismo , Sudoeste de Estados Unidos , Especificidad de la Especie , Succinatos/análisis , Succinatos/metabolismo , Zea mays/crecimiento & desarrollo , Zea mays/metabolismoRESUMEN
Growth in unconventional oil and gas has spurred concerns on environmental impact and interest in beneficial uses of produced water (PW), especially in arid regions such as the Permian Basin, the largest U.S. tight-oil producer. To evaluate environmental impact, treatment, and reuse potential, there is a need to characterize the compositional variability of PW. Although hydraulic fracturing has caused a significant increase in shale-oil production, there are no high-resolution organic composition data for the shale-oil PW from the Permian Basin or other shale-oil plays (Eagle Ford, Bakken, etc.). PW was collected from shale-oil wells in the Midland sub-basin of the Permian Basin. Molecular characterization was conducted using high-resolution solid phase micro extraction gas chromatography time-of-flight mass spectrometry. Approximately 1400 compounds were identified, and 327 compounds had a >70% library match. PW contained alkane, cyclohexane, cyclopentane, BTEX (benzene, toluene, ethylbenzene, and xylene), alkyl benzenes, propyl-benzene, and naphthalene. PW also contained heteroatomic compounds containing nitrogen, oxygen, and sulfur. 3D van Krevelen and double bond equivalence versus carbon number analyses were used to evaluate molecular variability. Source composition, as well as solubility, controlled the distribution of volatile compounds found in shale-oil PW. The salinity also increased with depth, ranging from 105 to 162 g/L total dissolved solids. These data fill a gap for shale-oil PW composition, the associated petroleomics plots provide a fingerprinting framework, and the results for the Permian shale-oil PW suggest that partial treatment of suspended solids and organics would support some beneficial uses such as onsite reuse and bio-energy production.
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Monitoreo del Ambiente/métodos , Yacimiento de Petróleo y Gas/química , Compuestos Orgánicos Volátiles/análisis , Aguas Residuales/química , Contaminantes Químicos del Agua/análisis , Cromatografía de Gases y Espectrometría de Masas , Minerales/análisis , TexasRESUMEN
Microalgae-based biofuels are promising sources of alternative energy, but improvements throughout the production process are required to establish them as economically feasible. One of the most influential improvements would be a significant increase in lipid yields, which could be achieved by altering the regulation of lipid biosynthesis and accumulation. Chlamydomonas reinhardtii accumulates oil (triacylglycerols, TAG) in response to nitrogen (N) deprivation. Although a few important regulatory genes have been identified that are involved in controlling this process, a global understanding of the larger regulatory network has not been developed. In order to uncover this network in this species, a combined omics (transcriptomic, proteomic and metabolomic) analysis was applied to cells grown in a time course experiment after a shift from N-replete to N-depleted conditions. Changes in transcript and protein levels of 414 predicted transcription factors (TFs) and transcriptional regulators (TRs) were monitored relative to other genes. The TF and TR genes were thus classified by two separate measures: up-regulated versus down-regulated and early response versus late response relative to two phases of polar lipid synthesis (before and after TAG biosynthesis initiation). Lipidomic and primary metabolite profiling generated compound accumulation levels that were integrated with the transcript dataset and TF profiling to produce a transcriptional regulatory network. Evaluation of this proposed regulatory network led to the identification of several regulatory hubs that control many aspects of cellular metabolism, from N assimilation and metabolism, to central metabolism, photosynthesis and lipid metabolism.
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Chlamydomonas reinhardtii/genética , Regulación de la Expresión Génica , Redes Reguladoras de Genes , Metabolismo de los Lípidos , Nitrógeno/deficiencia , Proteínas Algáceas/genética , Proteínas Algáceas/metabolismo , Chlamydomonas reinhardtii/metabolismo , Genoma , Metaboloma , Proteoma , TranscriptomaRESUMEN
Drastic alteration in macronutrients causes large changes in gene expression in the photosynthetic unicellular alga Chlamydomonas reinhardtii. Preliminary data suggested that cells follow a biphasic response to this change hinging on the initiation of lipid accumulation, and we hypothesized that drastic repatterning of metabolism also followed this biphasic modality. To test this hypothesis, transcriptomic, proteomic, and metabolite changes that occur under nitrogen (N) deprivation were analyzed. Eight sampling times were selected covering the progressive slowing of growth and induction of oil synthesis between 4 and 6 h after N deprivation. Results of the combined, systems-level investigation indicated that C. reinhardtii cells sense and respond on a large scale within 30 min to a switch to N-deprived conditions turning on a largely gluconeogenic metabolic state, which then transitions to a glycolytic stage between 4 and 6 h after N depletion. This nitrogen-sensing system is transduced to carbon- and nitrogen-responsive pathways, leading to down-regulation of carbon assimilation and chlorophyll biosynthesis, and an increase in nitrogen metabolism and lipid biosynthesis. For example, the expression of nearly all the enzymes for assimilating nitrogen from ammonium, nitrate, nitrite, urea, formamide/acetamide, purines, pyrimidines, polyamines, amino acids and proteins increased significantly. Although arginine biosynthesis enzymes were also rapidly up-regulated, arginine pool size changes and isotopic labeling results indicated no increased flux through this pathway.
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Chlamydomonas reinhardtii/metabolismo , Nitrógeno/metabolismo , Triglicéridos/biosíntesis , Adaptación Fisiológica , Arginina/biosíntesis , Chlamydomonas reinhardtii/crecimiento & desarrollo , Chlamydomonas reinhardtii/ultraestructura , Perfilación de la Expresión Génica , Poliaminas/metabolismo , Proteínas/metabolismo , Biología de Sistemas , Regulación hacia ArribaRESUMEN
The accumulation of carbon storage compounds by many unicellular algae after nutrient deprivation occurs despite declines in their photosynthetic apparatus. To understand the regulation and roles of photosynthesis during this potentially bioenergetically valuable process, we analyzed photosynthetic structure and function after nitrogen deprivation in the model alga Chlamydomonas reinhardtii. Transcriptomic, proteomic, metabolite, and lipid profiling and microscopic time course data were combined with multiple measures of photosynthetic function. Levels of transcripts and proteins of photosystems I and II and most antenna genes fell with differing trajectories; thylakoid membrane lipid levels decreased, while their proportions remained similar and thylakoid membrane organization appeared to be preserved. Cellular chlorophyll (Chl) content decreased more than 2-fold within 24 h, and we conclude from transcript protein and (13)C labeling rates that Chl synthesis was down-regulated both pre- and posttranslationally and that Chl levels fell because of a rapid cessation in synthesis and dilution by cellular growth rather than because of degradation. Photosynthetically driven oxygen production and the efficiency of photosystem II as well as P700(+) reduction and electrochromic shift kinetics all decreased over the time course, without evidence of substantial energy overflow. The results also indicate that linear electron flow fell approximately 15% more than cyclic flow over the first 24 h. Comparing Calvin-Benson cycle transcript and enzyme levels with changes in photosynthetic (13)CO2 incorporation rates also pointed to a coordinated multilevel down-regulation of photosynthetic fluxes during starch synthesis before the induction of high triacylglycerol accumulation rates.