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Fungal Biol ; 122(2-3): 182-189, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29458721

RESUMEN

Oomycetes are eukaryotic pathogens infecting animals and plants. Amongst them Saprolegnia parasitica is a fish pathogenic oomycete causing devastating losses in the aquaculture industry. To secure fish supply, new drugs are in high demand and since fish experiments are time consuming, expensive and involve animal welfare issues the search for adequate model systems is essential. Galleria mellonella serves as a heterologous host model for bacterial and fungal infections. This study extends the use of G. mellonella for studying infections with oomycetes. Saprolegniales are highly pathogenic to the insects while in contrast, the plant pathogen Phytophthora infestans showed no pathogenicity. Melanisation of hyphae below the cuticle allowed direct macroscopic monitoring of disease progression. However, the melanin response is not systemic as for other pathogens but instead is very local. The mortality of the larvae is dose-dependent and can be induced by cysts or regenerating protoplasts as an alternative source of inoculation.


Asunto(s)
Modelos Animales de Enfermedad , Peces/parasitología , Mariposas Nocturnas/parasitología , Saprolegnia/patogenicidad , Animales , Larva/parasitología , Mariposas Nocturnas/inmunología , Fenotipo , Protoplastos , Virulencia
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