RESUMEN
The present study evaluated the repercussions of magnetopriming on the root system architecture of soybean plants subjected to arsenic toxicity using synchrotron radiation source based micro-computed tomography (SR-µCT). This will be used evey where as abbreviation for the technique for three-dimensional imaging. Seeds of soybean were exposed to the static magnetic field (SMF) of strength (200 mT) for 1h prior to sowing. Magnetoprimed and non-primed seeds were grown for 1 month in a soil-sand mixture containing four different levels of sodium arsenate (0, 5, 10, and 50 mg As kg-1 soil). The results showed that arsenic adversely affects the root growth in non-primed plants by reducing their root length, root biomass, root hair, size and number of root nodules, where the damaging effect of As was observed maximum at higher concentrations (10 and 50 mg As kg-1 soil). However, a significant improvement in root morphology was detected in magnetoprimed plants where SMF pretreatment enhanced the root length, root biomass, pore diameter of cortical cells, root hair formation, lateral roots branching, and size of root nodules and girth of primary roots. Qualitative analysis of x-ray micro-CT images showed that arsenic toxicity damaged the epidermal and cortical layers of the root as well as reduced the pore diameter of the cortical cells. However, the diameter of cortical cells pores in magnetoprimed plants was observed higher as compared to plants emerged from non-primed seeds at all level of As toxicity. Thus, the study suggested that magnetopriming has the potential to attenuate the toxic effect of As and could be employed as a pre-sowing treatment to reduce the phytotoxic effects of metal ions in plants by improving root architecture and root tolerance index. This study is the very first exploration of the potential benefits of magnetopriming in mitigating the toxicity of metals (As) in plant roots utilizing the micro-CT technique.
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In this study, the role of the signalling molecule nitric oxide (NO) in magnetopriming-mediated induction of salinity tolerance in soybean seeds is established. The cross-talk of NO with germination-related hormones gibberellic acid (GA), abscisic acid (ABA) and auxin (IAA) for their ability to reduce the Na+/K+ ratio in the seeds germinating under salinity is highlighted. Salt tolerance index was significantly high for seedlings emerging from magnetoprimed seeds and sodium nitroprusside (SNP, NO-donor) treatment. The NO and superoxide (O2â¢-) levels were also increased in both of these treatments under non-saline and saline conditions. NO generation through nitrate reductase (NR) and nitric oxide synthase-like (NOS-like) pathways indicated the major contribution of NO from the NR-catalysed reaction. The relative expression of genes involved in the NO biosynthetic pathways reiterated the indulgence of NR in NO in magnetoprimed seeds, as a 3.86-fold increase in expression was observed over unprimed seeds under salinity. A 23.26-fold increase in relative expression of NR genes by the NO donor (SNP) was observed under salinity, while the NR inhibitor (sodium tungstate, ST) caused maximum reduction in expression of NR genes as compared to other inhibitors [L-NAME (N(G)-nitro-L-arginine methyl ester; inhibitor of nitric oxide synthase-like enzyme) and DPI (diphenylene iodonium; NADPH oxidase inhibitor)]. The ratio of ABA/GA and IAA/GA decreased in magnetoprimed and NO donor-treated seeds, suggesting homeostasis amongst hormones during germination under salinity. The magnetoprimed seeds showed low Na+/K+ ratio in all treatments irrespective of NO inhibitors. Altogether, our results indicate that a balance of ABA, GA and IAA is maintained by the signalling molecule NO in magnetoprimed seeds which lowers the Na+/K+ ratio to offset the adverse effects of salinity in soybean seeds.
Asunto(s)
Glycine max , Reguladores del Crecimiento de las Plantas , Ácido Abscísico/metabolismo , Ácido Abscísico/farmacología , Hormonas/metabolismo , Óxido Nítrico/metabolismo , Donantes de Óxido Nítrico/farmacología , Óxido Nítrico Sintasa/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Reguladores del Crecimiento de las Plantas/farmacología , Estrés Salino , Semillas/metabolismoRESUMEN
The combined response of exclusion of solar ultraviolet radiation (UV-A+B and UV-B) and static magnetic field (SMF) pre-treatment of 200 mT for 1 h were studied on soybean (Glycine max) leaves using synchrotron imaging. The seeds of soybean with and without SMF pre-treatment were sown in nursery bags kept in iron meshes where UV-A+B (280-400 nm) and UV-B (280-315 nm) from solar radiation were filtered through a polyester filters. Two controls were planned, one with polythene filter controls (FC)- which allows all the UV (280-400 nm); the other control had no filter used (open control-OC). Midrib regions of the intact third trifoliate leaves were imaged using the phase-contrast imaging technique at BL-4, Indus-2 synchrotron radiation source. The solar UV exclusion results suggest that ambient UV caused a reduction in leaf growth which ultimately reduced the photosynthesis in soybean seedlings, while SMF treatment caused enhancement of leaf growth along with photosynthesis even under the presence of ambient UV-B stress. The width of midrib and second-order veins, length of the second-order veins, leaf vein density, and the density of third-order veins obtained from the quantitative image analysis showed an enhancement in the leaves of plants that emerged from SMF pre-treated seeds as compared to untreated ones grown in open control and filter control conditions (in the presence of ambient UV stress). SMF pre-treated seeds along with UV-A+B and UV-B exclusion also showed significant enhancements in leaf parameters as compared to the UV excluded untreated leaves. Our results suggested that SMF-pretreatment of seeds diminishes the ambient UV-induced adverse effects on soybean.
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Glycine max/efectos de la radiación , Campos Magnéticos , Hojas de la Planta/efectos de la radiación , Sincrotrones , Rayos Ultravioleta , Hojas de la Planta/anatomía & histología , Estomas de Plantas/anatomía & histología , Estomas de Plantas/fisiología , Estomas de Plantas/efectos de la radiación , Haz Vascular de Plantas/anatomía & histología , Haz Vascular de Plantas/efectos de la radiación , Espectrofotometría UltravioletaRESUMEN
Whiteflies are a group of universally occurring insects that are considered to be a serious pest in their own way for causing both direct and indirect damages to crops. A few of them serve as vectors of plant viruses that are detrimental to the crop in question and cause an actual loss in productivity. A lot of attention is focused on pest control measures under the umbrella of IPM. In this review, we attempt to summarize the existing literature on how and why whiteflies are a serious concern for agriculture and society. We reviewed why there could be a need for fresh insight into the ways and means with which the pest can be combated. Here, we have emphasized next-generation strategies based on macromolecules, i.e., RNA interference and genetic engineering (for the expression of anti-whitefly proteins), as these strategies possess the greatest scope for research and improvement in the future. Recent scientific efforts based on nanotechnology and genome editing, which seem to offer great potential for whitefly/crop pest control, have been discussed. Comprehensive apprehensions related to obstacles in the path of taking lab-ready technologies into the farmers' field have also been highlighted. Although the use of RNAi, GM crops, nanotechnologies, for the control of whiteflies needs to be evaluated in the field, there is an emerging range of possible applications with promising prospects for the control of these tiny flies that are mighty pests.
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The study was performed to analyze the impact of seed pretreatment by static magnetic field (SMF) of 200 mT for 1 h on photosynthetic performance of soybean (Glycine max) seedlings under ambient (aUV-B) and supplemental ultraviolet-B (a+sUV-B) stress. Ambient and supplemental UV-B were found to decrease the plant growth, chlorophyll concentration, PSII efficiency, selected JIP-test parameters such as Fv/Fm, φEo, ΔV(I-P), PIABS, PItotal, and rate of photosynthesis in the leaves of soybean seedlings emerged from untreated (UT) seeds. aUV-B and a+sUV-B were observed to increase the synthesis of UV-B-absorbing substances (UAS), reactive oxygen species (ROS) like superoxide radical (O2·-) and hydrogen peroxide (H2O2), antioxidants like ascorbic acid and α-tocopherol and decrease the nitrate reductase (NR) activity; subsequently, it results in a decreased rate of photosynthesis, biomass accumulation, and yield. However, our results provided evidence that SMF pretreatment increased the tolerance of soybean seedlings to UV-B radiation by increased NO content and NR activity; higher efficiency of PSII, higher values of φEo, ΔV(I-P), PIABS, and PItotal, decreased intercellular CO2 concentration, lower amount of UAS, ROS, and antioxidants that consequently improve the yield of soybean plants under aUV-B as well as a+sUV-B stress. Thus, our results suggested that SMF pretreatment mitigates the adverse effects of UV-B stress by the enhancement in photosynthetic performance along with higher NO content which may be able to protect the plants from the deleterious effects of oxidative stress caused by UV-B irradiation.
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Glycine max , Peróxido de Hidrógeno , Clorofila , Campos Magnéticos , Fotosíntesis , Hojas de la Planta , Plantones , Rayos UltravioletaRESUMEN
We have evaluated the contribution of nitric oxide (NO) in static magnetic field (SMF-200 mT for 1h) induced tolerance towards UV-B stress in soybean seedlings using various NO modulators like sodium nitroprusside (SNP), inhibitor of nitrate reductase (NR) sodium tungstate (ST), NO synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME) and diphenylene iodonium (DPI) a NADPH oxidase inhibitor. The UV-B exposure significantly reduced germination, seedling growth together with activities of total amylase, NOS and NR in seedlings from un-primed seeds whereas SMF-primed seedlings showed significant enhancement in all these parameters along with higher level of NO/ROS. The supply of NO donor, SNP further improved all the seedlings parameters in un-primed and SMF-primed seeds after UV-B exposure. While ST, L-NAME and DPI significantly reduced the SMF-induced seedling performance after UV-B exposure. The gene expression study also showed significant up-regulation of α-amylase (GmAMY1, GmAMY2), nitric oxide synthase (GmNOS2) and nitrate reductase (GmNR2) encoding genes in UV-B exposed SMF-primed seedlings over un-primed seedlings. In particular, SNP+UV-B treatment enhanced the GmNOS2 expression in both unprimed (31.9-fold) and SMF-primed (93.2-fold) seedlings in comparison to their respective controls of CK+UV-B. In contrast, L-NAME+UV-B treatment reduced the SMF-induced GmNOS2 expression (4.8-fold) and NOS activity (76%). It confirmed that NO may be the key signaling molecule in SMF stimulated tolerance towards UV-B stress during early seedling growth and NOS may possibly be accountable for SMF-triggered NO production in soybean seedlings exposed to UV-B irradiations.
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Germinación/efectos de la radiación , Glycine max/fisiología , Magnetismo , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico/metabolismo , Plantones/efectos de la radiación , Rayos Ultravioleta , Especies Reactivas de Oxígeno/metabolismo , Plantones/crecimiento & desarrollo , Glycine max/crecimiento & desarrollo , Glycine max/efectos de la radiación , alfa-Amilasas/metabolismoRESUMEN
This study investigates the effect of static magnetic field (SMF) pre-treatment in ameliorating arsenic (As) toxicity in soybean plants in relation to growth, photosynthesis and water transport through leaf venation. Soybean (Glycine max variety JS-9560) seeds pre-treated with SMF (200 mT for 1 h) were grown in four levels of arsenate-polluted soil (As(V); 0, 5, 10 and 50 mg kg-1 ) in order to find out the impact of magnetopriming on plant tolerance against As toxicity. Quantitative image analysis of soybean leaf venation showed a narrowing in the width of midrib with increasing As(V) contamination in non-primed seeds. The morphological variations are also supported by the physiological parameters such as reduction in efficiency of photosystem II, plant performance index, stomatal conductance and photosynthetic rate in the presence of As(V) for non-primed seeds. However, remarkable increase was observed in all the measured parameters by SMF pre-treatment at all the concentrations of As(V) used. Even for the highest concentration of As(V) (50 mg kg-1 soil), SMF pre-treatment caused significant enhancement in plant height (40%), area of third trifoliate leaves (40%), along with increase in width of the midrib (17%) and minor vein (13%), contributing to increase in the water uptake, that resulted in higher primary photochemistry of PSII (12%), performance index (50%), stomatal conductance (57%) and photosynthetic rate (33%) as compared to non-primed ones. Consequently, magnetopriming of dry seeds can be effectively used as pretreatment for reduction of As toxicity in soybean plants.
Asunto(s)
Arsénico , Glycine max , Arsénico/toxicidad , Clorofila , Fotosíntesis , Hojas de la Planta , SincrotronesRESUMEN
Crop yield can be raised by establishment of adequate plant stand using seeds with high germination ratio and vigor. Various pre-sowing treatments are adopted to achieve this objective. One of these approaches is the exposure of seeds to a low-to-medium level magnetic field (MF), in pulsed and continuous modes, as they have shown positive results in a number of crop seeds. On the basis of the sensitivity of plants to MF, different types of MF have been used for magnetopriming studies, such as weak static homogeneous magnetic fields (0-100 µT, including GMF), strong homogeneous magnetic fields (milliTesla to Tesla), and extremely low frequency (ELF) magnetic fields of low-to-moderate (several hundred µT) magnetic flux densities. The agronomic application of MFs in plants has shown potential in altering conventional plant production systems; increasing mean germination rates, and root and shoot growth; having high productivity; increasing photosynthetic pigment content; and intensifying cell division, as well as water and nutrient uptake. Furthermore, different studies suggest that MFs prevent the large injuries produced/inflicted by diseases and pests on agricultural crops and other economically important plants and assist in reducing the oxidative damage in plants caused by stress situations. An improved understanding of the interactions between the MF and the plant responses could revolutionize crop production through increased resistance to disease and stress conditions, as well as the superiority of nutrient and water utilization, resulting in the improvement of crop yield. In this review, we summarize the potential applications of MF and the key processes involved in agronomic applications. Furthermore, in order to ensure both the safe usage and acceptance of this new opportunity, the adverse effects are also discussed.
RESUMEN
The experiments were conducted for the estimation of the mitigating effect of the static magnetic field (SMF of 200 mT for 1 h) treatment on soybean under ambient UV-B stress. The SMF treated (MT) and untreated (UT) seeds were grown inside iron cages covered with polyester filters for the purpose to filter UV-A + B (< 400 nm) and UV-B (< 300 nm) radiations, polythene filter control (FC) transparent for UV (280-400 nm), and open controls (OC) were without any filters. Our results indicated that specific leaf weight, efficiency of PS II, activity of carbonic anhydrase (CA) and nitrogenase (NRA), nucleic acid and protein content, nitric oxide (NO) and yield were significantly decreased in plants of untreated seeds under UV-B stress. SMF treatment to the soybean seeds was observed to mitigate the adverse effect of ambient UV-B with a significant enhancement in above-measured parameters in plants when compared with plants of untreated seeds grown under OC/FC conditions. Chlorophyll a fluorescence transition curve (OJIP-curve) from SMF treated and UV excluded plants has shown a higher fluorescence yield especially for I-P phase as compared to the plants grown in ambient UV-B stress. Reduction in the level of superoxide anion radicle ( O 2 · - ), hydrogen peroxide (H2O2), malondialdehyde (MDA) and proline content with a remarkable increase in DNA, RNA, protein and NO content, increased photosynthetic efficiency and nitrogen fixation in the leaves of soybean suggested the ameliorating effect of SMF pre-treatment against ambient UV-B induced damage. Consequently, SMF-pretreatment increased the tolerance of soybean seedlings to ambient UV-B stress as compared to the untreated seeds. The increase in carbon and nitrogen fixation ability due to SMF pre-treatment and the omission of solar UV radiation impact can be a direction for the purpose to improve the crop yield. Evaluation of the consequences of SMF treated seeds under ambient UV-B stress, and the plants from untreated seeds under solar UV exclusion indicated parallelism among the two effects.
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In the present study, experiments were performed to investigate the role of nitric oxide (NO) in magnetopriming-induced seed germination and early growth characteristics of soybean (Glycine max) seedlings under salt stress. The NO donor (sodium nitroprusside, SNP), NO scavenger (2-[4-carboxyphenyl]-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, CPTIO), inhibitors of nitrate reductase (sodium tungstate, ST) or NO synthase (N-nitro-L-Arg-methyl ester, LNAME) and NADPH oxidase inhibitor (diphenylene iodonium, DPI) have been used to measure the role of NO in the alleviation of salinity stress by static magnetic field (SMF of 200 mT, 1 h). Salt stress (50 mM NaCl) significantly reduced germination and early growth of seedlings emerged from non-primed seeds. Pre-treatment of seeds with SMF positively stimulated the germination and consequently promoted the seedling growth. ST, LNAME, CPTIO and DPI significantly decreased the growth of seedling, activities of α-amylase, protease and nitrate reductase (NR), hydrogen peroxide (H2 O2 ), superoxide (O2 â¢- ) and NO content in roots of seedlings emerged from non-primed and SMF-primed seeds. However, the extent of reduction was higher with ST in seedlings of SMF-primed seeds under both conditions, whereas SNP promoted all the studied parameters. Moreover, the generation of NO was also confirmed microscopically using a membrane permanent fluorochrome (4-5-diaminofluorescein diacetate [DAF-2 DA]). Further, analysis showed that SMF enhanced the NR activity and triggered the NO production and NR was maximally decreased by ST as compared to LNAME, CPTIO and DPI. Thus, in addition to ROS, NO might be one of the important signaling molecules in magnetopriming-induced salt tolerance in soybean and NR may be responsible for SMF-triggered NO generation in roots of soybean.
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Glycine max/enzimología , Campos Magnéticos , Nitrato-Reductasa/fisiología , Óxido Nítrico/biosíntesis , Tolerancia a la Sal , Germinación , Plantones/enzimología , Plantones/fisiología , Transducción de Señal , Glycine max/fisiologíaRESUMEN
BACKGROUND: Understanding the attitude toward rural health care among future medical graduates, the health workforce of the near future, is an important exercise. OBJECTIVE: The objective of this study is to understand the attitude of third year MBBS students in a Government Medical College of Chhattisgarh toward rural health services. METHODOLOGY: A cross-sectional study was conducted in 2014 using a semi-open-ended questionnaire. The analysis was primarily descriptive, and nonparametric test of significance was used. RESULTS: Of a total of 293 students, 263 (89.7%) rated the current rural health services to be unsatisfactory. Nearly 44% students were willing to serve in the rural area. There was no statistical difference among willing and nonwilling 3rd year Part I students regarding willingness to join rural services but mostly not willing among 3rd year Part II. Majority (66.2%) were only willing to work in rural areas for <1 year. The oft-mentioned reason was reservation or added marks in postgraduate entrance examination by more than two-third respondents, "health services for the poor" by nearly two-third respondents and followed by "gain of knowledge about rural people and their diseases." Nearly 10% would-be medical graduates perceived no apparent benefit. The greatest perceived disadvantage was "lack of infrastructural facilities" by more than 80% of the respondents, while "lack of education opportunities for children and basic amenities for family members" was a concern for nearly three-fourth of respondents. Less than half of the respondents thought that there were no career growth opportunities in rural practice. CONCLUSION: If the identified perceived factors of nonwillingness are taken care off, it would lead to a drastic increase in the number of doctors joining rural service. Not only that but also this would lead to more doctors staying in their position for a longer duration than currently mandated. This would require a relook at the preexisting health policies and adapting them accordingly to retain the services of rural health workers.
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Apoptosis is a general phenomenon of all multicellular organisms and caspases form a group of important proteins central to suicide of cells. Pathologies like cancer, Myocardial infarction, Stroke, Sepsis, Alzheimer's, Psoriasis, Parkinson and Huntington diseases are often associated with change in caspase 3 mediated apoptosis and therefore, caspases may serve as potential inhibitory targets for drug development. In the present study, two series of synthetic acetylated tetrapeptides containing aldehyde and fluromethyl keto groups respectively at the C terminus were proposed. All these compounds were evaluated for binding affinity against caspase 3 structure. In series 1 compound Ac-DEHD-CHO demonstrated appreciable and high binding affinity (Rerank Score: -138.899) against caspase 3. While in series 2 it was Ac-WEVD-FMK which showed higher binding affinity (Rerank Score: -139.317). Further these two compounds met ADMET properties and demonstrated to be non- toxic.
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Apoptosis , Caspasa 3 , Inhibidores de Caspasas , Simulación del Acoplamiento Molecular , Aldehídos , Animales , Simulación por Computador , Descubrimiento de Drogas , Humanos , MamíferosRESUMEN
Mercury (Hg), a potent metallic toxicant, is known for having inhibitory effect on chlorophyll biosynthesis. In vivo supply of HgCl(2) inhibited 5-aminolevulinic acid dehydratase (ALAD, EC 4.2.1.24) activity in excised greening maize (Zea mays) leaf segments. The inhibition caused by Hg was alleviated by addition of KNO(3). Amongst the nutrients and metabolites tested, NH(4)Cl and sucrose increased the inhibitory effect of Hg on enzyme activity, while glutamine and glutathione decreased it. The inhibitors, levulinic acid and 5,5' dithio bis 2-nitrobenzoic acid, also reduced the % inhibition of enzyme activity caused by Hg supply. In vitro inclusion of Hg during assay of the enzyme preparations obtained from the tissue treated without Hg (-Hg enzyme) and with Hg (+Hg enzyme) caused the inhibition of -Hg enzyme but activation of +Hg enzyme. Almost similar trend was observed for the in vitro inclusion of Hg in the presence of levulinic acid. It is suggested that two forms of enzyme exist in Hg-treated tissue, i.e. the usual Mg dependent form and an unusual Hg modified form. Kinetic studies for the two enzymes, -Hg enzyme and +Hg enzyme, involving the effect of varying concentrations of δ-aminolevulinic acid yielded distinct apparent K(m) and apparent V(max) values being 532 µM and 118 units g(-1) fr. wt., respectively, for -Hg enzyme and 347 µM and 52 units g(-1) fr. wt., respectively, for +Hg enzyme indicating that +Hg enzyme has higher affinity for δ-aminolevulinic acid but lower activity as compared to the -Hg enzyme.
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Mercurio/toxicidad , Hojas de la Planta/enzimología , Porfobilinógeno Sintasa/antagonistas & inhibidores , Zea mays/enzimología , Activación Enzimática/efectos de los fármacos , Activación Enzimática/genética , Glutamina/genética , Glutamina/metabolismo , Glutatión/genética , Glutatión/metabolismo , Hojas de la Planta/genética , Porfobilinógeno Sintasa/genética , Porfobilinógeno Sintasa/metabolismo , Zea mays/genéticaAsunto(s)
Presión Sanguínea , Frecuencia Cardíaca , Terapia por Relajación , Yoga , Adulto , Femenino , Humanos , India , Masculino , Persona de Mediana Edad , Factores de TiempoRESUMEN
Supply of cadmium chloride (0.5 mM) inhibited chlorophyll formation in greening maize leaf segments, while lower concentration of Cd (0.01 mM) slightly enhanced it. Inclusion of 2-oxoglutarate (2-OG, 0.1-10 mM) in the incubation mixture increased chlorophyll content in the absence as well as presence of Cd. Substantial inhibition of chlorophyll formation by Cd was observed at longer treatment both in the absence and presence of 2-OG. When the tissue was pre-incubated with 2-OG or Cd, the inhibition (%) of chlorophyll formation by Cd was lowered in the presence of 2-OG. Treatment with Cd inhibited ALAD activity and ALA formation and the inhibition (%) of ALA formation by Cd was strongly reduced in the presence of 2-OG. Glutamate dehydrogenase (GDH) activity was increased by the supply of Cd both in the absence as well as presence of 2-OG. In the presence of 2-OG, Cd supply significantly increased glutamate synthase (GOGAT) activity and reduced inhibition (%) of glutamine synthetase (GS) activity. The results suggested the involvement of the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway of ammonia assimilation to provide the precursor, glutamate, for ALA synthesis under Cd toxicity and 2-OG supplementation.
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Cloruro de Cadmio/farmacología , Clorofila/antagonistas & inhibidores , Ácidos Cetoglutáricos/farmacología , Porfobilinógeno Sintasa/antagonistas & inhibidores , Zea mays/efectos de los fármacos , Ácido Aminolevulínico/antagonistas & inhibidores , Ácido Aminolevulínico/metabolismo , Clorofila/biosíntesis , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/enzimología , Compuestos de Amonio Cuaternario/metabolismo , Zea mays/enzimologíaRESUMEN
In vivo as well as in vitro supply of sodium arsenate inhibited the 5-Amino levulinic acid dehydratase (5-aminolevulinate-hydrolyase EC 4.2.1.24, ALAD) activity in excised etiolated maize leaf segments during greening. The percent inhibition of enzyme activity by arsenate (As) was reduced by the supply of KNO3, but it was increased by the glutamine and GSH. Various inhibitors, such as, chloramphenicol, cycloheximide and LA, decreased the % inhibition of enzyme activity by As. The % inhibition of enzyme activity was also reduced by in vivo supply of DTNB. The enzyme activity was reduced substantially by in vitro inclusion of LA, both in the absence and presence of As. In vitro inclusion of DTNB and GSH inhibited the enzyme activity extracted from leaf segments treated without arsenate (-As enzyme) and caused respectively no effect and stimulatory effect on arsenate treated enzyme (+As enzyme). Increasing concentration of ALA during assay increased the activity of -As enzyme and +As enzyme to different extent, but double reciprocal plots for both the enzymes were biphasic and yielded distinct S0.5 values for the two enzymes (-As enzyme, 40 micromol/L and +As enzyme, 145 micromol/L) at lower concentration range of ALA only. It is suggested that As inhibits ALAD activity in greening maize leaf segments by affecting its thiol groups and/or binding of ALA to the enzyme.
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Arsénico/toxicidad , Porfobilinógeno Sintasa/antagonistas & inhibidores , Zea mays/efectos de los fármacos , Zea mays/enzimología , Ácido Aminolevulínico/farmacología , Arseniatos/toxicidad , Ácido Ditionitrobenzoico/farmacología , Inhibidores Enzimáticos/toxicidad , Glutatión/farmacología , Técnicas In Vitro , Cinética , Ácidos Levulínicos/farmacología , Nitratos/farmacología , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/enzimología , Compuestos de Potasio/farmacología , Zea mays/metabolismoRESUMEN
Mercury (0.01-1.0 mM) inhibited chlorophyll formation in greening maize leaf segments. However, supplementing incubation medium with 2-oxoglutarate, maintained substantially higher level of chlorophyll in absence of metal after an initial period of 8 hr. On preincubation of leaf segments with HgCl2, per cent inhibition of chlorophyll synthesis by metal was same in the presence and absence of 2-oxoglutarate. Supply of 2-oxoglutarate (0.1-10.0 mM) exerted concentration dependent effect on chlorophyll formation in absence or presence of metal. Increase in delta-amino levulinic acid dehydratase as well as NADH-glutamate synthase activity and decrease in NADH-glutamate dehydrogenase activity by 2-oxoglutarate in the presence of Hg suggested that glutamate for delta-amino levulinic acid synthesis could be made available from NH4+ assimilation via., glutamine synthetase/glutamate synthase pathway during mercury toxicity.