RESUMEN
Diabetic patients with poorly controlled blood glucose have frequent and persistent bacterial infections particularly those infecting the skin, such as Staphylococcus aureus and S. epidermidis. The function of phagocytes of diabetic patients is believed to be impaired due to hyperglycemia, leading to suboptimal immune response to clear acute infection. The present study investigated interleukin (IL)-1beta expression by diabetic patients' monocytes (n = 22) experimentally infected with S. aureus compared with that from healthy subjects (n = 30). In addition, the in vitro effect of hyperglycemia on IL-1beta expression by monocytes from normal subjects (n = 18) stimulated with S. aureus and S. epidermidis was investigated. Monocytes from diabetic patients, stimulated or not with S. aureus, express significantly lower levels of IL-1beta than those from healthy subjects. In vitro hyperglycemia did not affect IL-1beta expression by unstimulated monocytes. However, at the same levels of glucose normal monocytes stimulated with S. aureus produce significantly higher IL-1beta than those stimulated with S. epidermidis. These findings suggest that diabetic patients have abnormally lower IL-1beta expression and hyperglycemia is related to abnormal expression of IL-1beta by monocytes, which could lead to enhanced susceptibility to infection by the more virulent bacteria.
Asunto(s)
Complicaciones de la Diabetes/inmunología , Hiperglucemia/inmunología , Interleucina-1beta/inmunología , Infecciones Cutáneas Estafilocócicas/inmunología , Adulto , Complicaciones de la Diabetes/microbiología , Humanos , Hiperglucemia/complicaciones , Hiperglucemia/etiología , Técnicas In Vitro , Persona de Mediana Edad , Monocitos/inmunología , Monocitos/fisiología , Fagocitos/inmunología , Fagocitos/fisiología , Infecciones Cutáneas Estafilocócicas/microbiología , Staphylococcus aureus/inmunología , Staphylococcus aureus/aislamiento & purificación , Staphylococcus epidermidis/inmunología , Staphylococcus epidermidis/aislamiento & purificación , TailandiaRESUMEN
Burkholderia pseudomallei is the causative agent of melioidosis. One of the main risk factors for B. pseudomallei infection in endemic areas is diabetes mellitus. The present study investigated IL-17 mRNA and protein expression by peripheral blood mononuclear cells in response to B. pseudomallei infection in 10 diabetic patients in comparison to 10 healthy blood donors. The IL-17 expression in diabetic patients was significantly lower (p < 0.05) than in the controls. However, IL-23 mRNA expression of the 2 groups was comparable. The present findings suggest that melioidosis affects T cell IL-17 production and that patients with diabetes mellitus have a defective IL-17 production in response to this type of infection.